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The map label for this gene is glnG [H]

Identifier: 91787691

GI number: 91787691

Start: 1873612

End: 1875096

Strand: Direct

Name: glnG [H]

Synonym: Bpro_1810

Alternate gene names: 91787691

Gene position: 1873612-1875096 (Clockwise)

Preceding gene: 91787690

Following gene: 91787695

Centisome position: 36.03

GC content: 63.37

Gene sequence:

>1485_bases
ATGAAACCGATTTGGATCGTTGACGATGACCAGTCCATCCGGTTTGTTCTGGAGAAGGCCCTGCTTCGTGAGGATCTTCC
TACGCGCAGTTTCACCAACCCCCGTGAGGTCCTCCTGGCGCTGGAAAACGCGACAGAGGAAGAGGGCCCGCAAGTCCTGG
TGAGCGACATTCGCATGCCCGGCGGGTCGGGGCTGGATCTGCTGGAGAAGGTCAAGCAAAAGCTTCCCGGCCTGCCGGTC
ATCATCATGACGGCGTTCTCCGACCTTGACAGCGCTGTTTCTGCTTTTCAGGGCGGCGCTTTCGAATACCTGCCCAAGCC
CTTCGACCTCCCAAAAGCGGTGGAACTCATACGCCGTGCGGTGGAAGAAAGCCAGCGCGAGGAGGTGGCCGAGGAGCGCA
TGGCGGCCGCGCCGGAAATGCTCGGCCAGGCGCCGGCGATGCAGGATGTGTTCCGTGCCATTGGCCGCTTGAGCCAGAGC
ATGGTGACGGTGATGATCACCGGCGAATCGGGGTCGGGCAAGGAACTGGTGGCGCGCGCGCTGCACAAGCACTCACCGCG
CGCCAACGGGCCTTTTGTGGCCATCAATACCGCGGCCATTCCCAAAGACCTGCTCGAGAGCGAACTCTTCGGTCATGAGC
GCGGCGCCTTCACCGGCGCGCAGACCATGCGGCGTGGCCGCTTTGAGCAGGCAGAGGGCGGAACGCTGTTCCTCGATGAA
ATCGGTGACATGCCCTTTGACCTGCAAACCCGCCTGCTCCGGGTGCTGAGCGATGGTCACTTTTACCGCGTGGGCGGCCA
CAACGCCGTCAAGGCCAATGTGCGCGTGATTGCCGCCACGCACCAGGACCTGGAGCAGCGCGTCAAGGAGGGTGGTTTTC
GCGAAGACCTCTTCCACCGGCTCAACGTGATTCGGCTGCGCCTGCCCGCGCTGCGCGAACGGCGCGAGGATGTGTTCATG
CTGACCCGCCATTTCCTGCAGCAAAGCGCCAAGCAGCTGGGTGTGGAGCCCAAGCGCATTTCCGATGCGGCGCTGCAGCA
ATTGAGCAACTTCAGTTTTCCCGGCAATGTGCGCCAGCTGGAAAACATCTGTCACTGGCTCACCGTGATGGCGCCCGCGC
AGGTGATTGAAGCCAAGGACCTGCCACCTGAAGTGCTGGGCGTGCCGCATGAACCGGCGCCGGGGGCTTCCGACAAAGCG
CCTCTGGCGCCCGTCACGCCGACGATGCATATGCCCGAGCCTGCCGTCTTGGCGGGGCCGGCGGCTGGCGAGCAGAATTG
GGAAGCCGGCCTTGAGGCGGAAGCCCTCGCTTTGCTGGCGGCCGGTCGCAGCGATGTGTGGGATGTGCTGACGCGCCGTT
TTGAGTCCCGGCTGATCCAGACGGCGCTGCTCAATACCCGCGGGCGTCGCATCGAGGCCGCCCAGAAGTTGGGTATTGGC
CGCAATACCATCACGCGCAAGATCCAGGAGCTGAATCTGGAGTAA

Upstream 100 bases:

>100_bases
TGCTGATACCGTTGCCTTGAGGTGTGTCCTTCCTACATGGCTTGACAAATAAAGCGCGATGACGCGAATGGCACCGAATG
TACAACAAGGACTGCACAAC

Downstream 100 bases:

>100_bases
AACCGGCTGCAATCCCTGAGGTTCCCCGGGGTGCAGTTCTATTTGCTATGGCAATCAGGGATGCGGAGCACACCCGATGG
TCCAGCCGTGCTGCACGGAC

Product: nitrogen metabolism transcriptional regulator NtrC

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MKPIWIVDDDQSIRFVLEKALLREDLPTRSFTNPREVLLALENATEEEGPQVLVSDIRMPGGSGLDLLEKVKQKLPGLPV
IIMTAFSDLDSAVSAFQGGAFEYLPKPFDLPKAVELIRRAVEESQREEVAEERMAAAPEMLGQAPAMQDVFRAIGRLSQS
MVTVMITGESGSGKELVARALHKHSPRANGPFVAINTAAIPKDLLESELFGHERGAFTGAQTMRRGRFEQAEGGTLFLDE
IGDMPFDLQTRLLRVLSDGHFYRVGGHNAVKANVRVIAATHQDLEQRVKEGGFREDLFHRLNVIRLRLPALRERREDVFM
LTRHFLQQSAKQLGVEPKRISDAALQQLSNFSFPGNVRQLENICHWLTVMAPAQVIEAKDLPPEVLGVPHEPAPGASDKA
PLAPVTPTMHMPEPAVLAGPAAGEQNWEAGLEAEALALLAAGRSDVWDVLTRRFESRLIQTALLNTRGRRIEAAQKLGIG
RNTITRKIQELNLE

Sequences:

>Translated_494_residues
MKPIWIVDDDQSIRFVLEKALLREDLPTRSFTNPREVLLALENATEEEGPQVLVSDIRMPGGSGLDLLEKVKQKLPGLPV
IIMTAFSDLDSAVSAFQGGAFEYLPKPFDLPKAVELIRRAVEESQREEVAEERMAAAPEMLGQAPAMQDVFRAIGRLSQS
MVTVMITGESGSGKELVARALHKHSPRANGPFVAINTAAIPKDLLESELFGHERGAFTGAQTMRRGRFEQAEGGTLFLDE
IGDMPFDLQTRLLRVLSDGHFYRVGGHNAVKANVRVIAATHQDLEQRVKEGGFREDLFHRLNVIRLRLPALRERREDVFM
LTRHFLQQSAKQLGVEPKRISDAALQQLSNFSFPGNVRQLENICHWLTVMAPAQVIEAKDLPPEVLGVPHEPAPGASDKA
PLAPVTPTMHMPEPAVLAGPAAGEQNWEAGLEAEALALLAAGRSDVWDVLTRRFESRLIQTALLNTRGRRIEAAQKLGIG
RNTITRKIQELNLE
>Mature_494_residues
MKPIWIVDDDQSIRFVLEKALLREDLPTRSFTNPREVLLALENATEEEGPQVLVSDIRMPGGSGLDLLEKVKQKLPGLPV
IIMTAFSDLDSAVSAFQGGAFEYLPKPFDLPKAVELIRRAVEESQREEVAEERMAAAPEMLGQAPAMQDVFRAIGRLSQS
MVTVMITGESGSGKELVARALHKHSPRANGPFVAINTAAIPKDLLESELFGHERGAFTGAQTMRRGRFEQAEGGTLFLDE
IGDMPFDLQTRLLRVLSDGHFYRVGGHNAVKANVRVIAATHQDLEQRVKEGGFREDLFHRLNVIRLRLPALRERREDVFM
LTRHFLQQSAKQLGVEPKRISDAALQQLSNFSFPGNVRQLENICHWLTVMAPAQVIEAKDLPPEVLGVPHEPAPGASDKA
PLAPVTPTMHMPEPAVLAGPAAGEQNWEAGLEAEALALLAAGRSDVWDVLTRRFESRLIQTALLNTRGRRIEAAQKLGIG
RNTITRKIQELNLE

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=493, Percent_Identity=55.9837728194726, Blast_Score=524, Evalue=1e-150,
Organism=Escherichia coli, GI1788550, Length=497, Percent_Identity=39.2354124748491, Blast_Score=349, Evalue=2e-97,
Organism=Escherichia coli, GI1790437, Length=488, Percent_Identity=37.7049180327869, Blast_Score=290, Evalue=2e-79,
Organism=Escherichia coli, GI1788905, Length=329, Percent_Identity=44.3768996960486, Blast_Score=275, Evalue=6e-75,
Organism=Escherichia coli, GI87082117, Length=225, Percent_Identity=52.8888888888889, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1789087, Length=259, Percent_Identity=47.1042471042471, Blast_Score=232, Evalue=5e-62,
Organism=Escherichia coli, GI1789233, Length=357, Percent_Identity=38.0952380952381, Blast_Score=228, Evalue=6e-61,
Organism=Escherichia coli, GI87082152, Length=265, Percent_Identity=46.4150943396226, Blast_Score=225, Evalue=4e-60,
Organism=Escherichia coli, GI1786524, Length=265, Percent_Identity=45.6603773584906, Blast_Score=208, Evalue=8e-55,
Organism=Escherichia coli, GI1787583, Length=385, Percent_Identity=33.7662337662338, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI87081872, Length=225, Percent_Identity=44, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1789828, Length=211, Percent_Identity=36.9668246445498, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI87081858, Length=290, Percent_Identity=30.3448275862069, Blast_Score=121, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 54634; Mature: 54634

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPIWIVDDDQSIRFVLEKALLREDLPTRSFTNPREVLLALENATEEEGPQVLVSDIRMP
CCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHCCC
GGSGLDLLEKVKQKLPGLPVIIMTAFSDLDSAVSAFQGGAFEYLPKPFDLPKAVELIRRA
CCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHH
VEESQREEVAEERMAAAPEMLGQAPAMQDVFRAIGRLSQSMVTVMITGESGSGKELVARA
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCEEEEEEECCCCCCHHHHHHH
LHKHSPRANGPFVAINTAAIPKDLLESELFGHERGAFTGAQTMRRGRFEQAEGGTLFLDE
HHHCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCEEEHHH
IGDMPFDLQTRLLRVLSDGHFYRVGGHNAVKANVRVIAATHQDLEQRVKEGGFREDLFHR
HCCCCHHHHHHHHHHHCCCCEEEECCCCEEEECEEEEEECHHHHHHHHHHCCCHHHHHHH
LNVIRLRLPALRERREDVFMLTRHFLQQSAKQLGVEPKRISDAALQQLSNFSFPGNVRQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHH
ENICHWLTVMAPAQVIEAKDLPPEVLGVPHEPAPGASDKAPLAPVTPTMHMPEPAVLAGP
HHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECC
AAGEQNWEAGLEAEALALLAAGRSDVWDVLTRRFESRLIQTALLNTRGRRIEAAQKLGIG
CCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
RNTITRKIQELNLE
HHHHHHHHHHHCCC
>Mature Secondary Structure
MKPIWIVDDDQSIRFVLEKALLREDLPTRSFTNPREVLLALENATEEEGPQVLVSDIRMP
CCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHCCC
GGSGLDLLEKVKQKLPGLPVIIMTAFSDLDSAVSAFQGGAFEYLPKPFDLPKAVELIRRA
CCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHH
VEESQREEVAEERMAAAPEMLGQAPAMQDVFRAIGRLSQSMVTVMITGESGSGKELVARA
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCEEEEEEECCCCCCHHHHHHH
LHKHSPRANGPFVAINTAAIPKDLLESELFGHERGAFTGAQTMRRGRFEQAEGGTLFLDE
HHHCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCEEEHHH
IGDMPFDLQTRLLRVLSDGHFYRVGGHNAVKANVRVIAATHQDLEQRVKEGGFREDLFHR
HCCCCHHHHHHHHHHHCCCCEEEECCCCEEEECEEEEEECHHHHHHHHHHCCCHHHHHHH
LNVIRLRLPALRERREDVFMLTRHFLQQSAKQLGVEPKRISDAALQQLSNFSFPGNVRQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHH
ENICHWLTVMAPAQVIEAKDLPPEVLGVPHEPAPGASDKAPLAPVTPTMHMPEPAVLAGP
HHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECC
AAGEQNWEAGLEAEALALLAAGRSDVWDVLTRRFESRLIQTALLNTRGRRIEAAQKLGIG
CCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
RNTITRKIQELNLE
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]