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The map label for this gene is rsuA [H]

Identifier: 91787681

GI number: 91787681

Start: 1862712

End: 1863431

Strand: Direct

Name: rsuA [H]

Synonym: Bpro_1800

Alternate gene names: 91787681

Gene position: 1862712-1863431 (Clockwise)

Preceding gene: 91787680

Following gene: 91787683

Centisome position: 35.82

GC content: 60.69

Gene sequence:

>720_bases
ATGCAATTACAGGACATTCTTTTCTCCCAGGGCTTTGGCACGCGGCGCGTTTGTGCGGGCCTGATTCAACAGGGTTTTGT
GGCCGTAGCTGGCATCCCCTGTACGGAGCCAGCGATGGAATTTGTAGCGGAAGACTTGCGCTTCACGGTGCAGGGGGTGG
CGTGGGAGTATCACGAAAAAGCCTACCTGATGCTGCATAAGCCGGCCGGTTATGAGTGTTCGCAGAAGCCCAGCACCTAC
CCCAGTATTTACACTTTGCTGCCGTCACCGATTCGCCAGCGTGGCGGTGGCGCTGCGGCGGGTGTACAGGCCGTGGGGCG
GCTGGACCAGGACACGACCGGCTTGCTGCTGCTGTCGGACGACGGCAAGTTCATTCACCGCATGAGCTCCCCCAGGCACC
ATGTACCCAAGGTTTATGAAGTGACCGCAAAGCATGCGGTGGACGACAGGCAGGTTCAGAAACTGCTGGCGGGTGTGGTG
CTGGATGACGATCCAAAGCCGGTGCGGGCCGCCGCCTGTGAAAAGACCGGCGAGCTGCAGTTAAGCCTGACGCTGACCGA
GGGCAAGTATCACCAGGTCAAACGCATGATTGCGGCCGTCGGCAACCGGGTGGAAGACCTGCACCGCTCGCAGCTGGGGG
GGCTGATGTTGCCTGAAGATCTCAAGCCCGGTCAGTGGCGCTGGCTCACGGCGCAGGATCTGTCCAGCATTTCGACCTGA

Upstream 100 bases:

>100_bases
CGTTCTCCATGGGTCGGGTACACGAGTCTCGGTTTCCGGTCCGTACCGCACCAGGCCGTATCTGGGGTTGCAGCCCGGCG
ACAATAGCGTCATGGCGACC

Downstream 100 bases:

>100_bases
TGCTTGCTTGCGCGGTTACGCTTCAGCTTCAGCTTCAGCTTCATGCTACCTTGCGCCCGTCAGCGTGCCTCAACTCACTC
GGCATACTGTTGGACTGGAT

Product: ribosomal small subunit pseudouridine synthase A

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MQLQDILFSQGFGTRRVCAGLIQQGFVAVAGIPCTEPAMEFVAEDLRFTVQGVAWEYHEKAYLMLHKPAGYECSQKPSTY
PSIYTLLPSPIRQRGGGAAAGVQAVGRLDQDTTGLLLLSDDGKFIHRMSSPRHHVPKVYEVTAKHAVDDRQVQKLLAGVV
LDDDPKPVRAAACEKTGELQLSLTLTEGKYHQVKRMIAAVGNRVEDLHRSQLGGLMLPEDLKPGQWRWLTAQDLSSIST

Sequences:

>Translated_239_residues
MQLQDILFSQGFGTRRVCAGLIQQGFVAVAGIPCTEPAMEFVAEDLRFTVQGVAWEYHEKAYLMLHKPAGYECSQKPSTY
PSIYTLLPSPIRQRGGGAAAGVQAVGRLDQDTTGLLLLSDDGKFIHRMSSPRHHVPKVYEVTAKHAVDDRQVQKLLAGVV
LDDDPKPVRAAACEKTGELQLSLTLTEGKYHQVKRMIAAVGNRVEDLHRSQLGGLMLPEDLKPGQWRWLTAQDLSSIST
>Mature_239_residues
MQLQDILFSQGFGTRRVCAGLIQQGFVAVAGIPCTEPAMEFVAEDLRFTVQGVAWEYHEKAYLMLHKPAGYECSQKPSTY
PSIYTLLPSPIRQRGGGAAAGVQAVGRLDQDTTGLLLLSDDGKFIHRMSSPRHHVPKVYEVTAKHAVDDRQVQKLLAGVV
LDDDPKPVRAAACEKTGELQLSLTLTEGKYHQVKRMIAAVGNRVEDLHRSQLGGLMLPEDLKPGQWRWLTAQDLSSIST

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=235, Percent_Identity=39.1489361702128, Blast_Score=161, Evalue=4e-41,
Organism=Escherichia coli, GI1787524, Length=234, Percent_Identity=32.9059829059829, Blast_Score=100, Evalue=6e-23,
Organism=Escherichia coli, GI87081838, Length=149, Percent_Identity=32.2147651006711, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70

Molecular weight: Translated: 26321; Mature: 26321

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLQDILFSQGFGTRRVCAGLIQQGFVAVAGIPCTEPAMEFVAEDLRFTVQGVAWEYHEK
CCHHHHHHHCCCCHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHEEEECCC
AYLMLHKPAGYECSQKPSTYPSIYTLLPSPIRQRGGGAAAGVQAVGRLDQDTTGLLLLSD
EEEEEECCCCCCCCCCCCCCCHHEECCCHHHHHCCCCHHHHHHHHHCCCCCCCEEEEECC
DGKFIHRMSSPRHHVPKVYEVTAKHAVDDRQVQKLLAGVVLDDDPKPVRAAACEKTGELQ
CCHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCEECCCCCCHHHHHHCCCCCEE
LSLTLTEGKYHQVKRMIAAVGNRVEDLHRSQLGGLMLPEDLKPGQWRWLTAQDLSSIST
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEHHHHHHCCC
>Mature Secondary Structure
MQLQDILFSQGFGTRRVCAGLIQQGFVAVAGIPCTEPAMEFVAEDLRFTVQGVAWEYHEK
CCHHHHHHHCCCCHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHEEEECCC
AYLMLHKPAGYECSQKPSTYPSIYTLLPSPIRQRGGGAAAGVQAVGRLDQDTTGLLLLSD
EEEEEECCCCCCCCCCCCCCCHHEECCCHHHHHCCCCHHHHHHHHHCCCCCCCEEEEECC
DGKFIHRMSSPRHHVPKVYEVTAKHAVDDRQVQKLLAGVVLDDDPKPVRAAACEKTGELQ
CCHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCEECCCCCCHHHHHHCCCCCEE
LSLTLTEGKYHQVKRMIAAVGNRVEDLHRSQLGGLMLPEDLKPGQWRWLTAQDLSSIST
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]