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The map label for this gene is hpcH [H]

Identifier: 91787369

GI number: 91787369

Start: 1527397

End: 1528170

Strand: Reverse

Name: hpcH [H]

Synonym: Bpro_1475

Alternate gene names: 91787369

Gene position: 1528170-1527397 (Counterclockwise)

Preceding gene: 91787370

Following gene: 91787366

Centisome position: 29.39

GC content: 66.28

Gene sequence:

>774_bases
ATGGAATTGCCAGTCAACCAATTCAAGAAAATGCTGCTGGGCGGCCATCAGCAGATAGGCCTGTGGTGCACGCTCTCCAG
CCCCTACGGCGTCGAGCTGCTAGCCGGTTCCGGCTTTGAATGGCTGCTGCTGGACACCGAGCATTCCCCCAGTGACGTCA
CCAACGTGCTGGCCCAGCTCCAGACCGTCGCGCCCTACCCGGTTTCCGCCGTGGTCAGGCCCGCCTGGAACGACCCGGTG
CTGATCAAGCGCTACCTCGATATCGGGGCGCAGACCCTGTTGATCCCCTATGTGCAGAACGCCGAGGAAGCCAGGCGGGC
CGTGCAGAGCATTACCTACCCCGGCGGTGGCATGCGCGGCGTGTCGGCGCTGACGCGGGCCTCGCGCTTCGGCCGTGTTC
CGAACTACGCCCAAACCTGCCGCGACGAGCTGTGCCTGCTGGTGCAGATTGAAACCCTGGAAGCGCTCGACAACATCGAA
GCGATTGCGGCCGTCGAGGGCGTGGACGGCATCTTCATCGGCCCGGGCGACCTGGCCGCCAGCATGGGTCACCTCGGCGA
GCCGGGCAACGCCGCCGTTGTCGCGAGGATCGAAGAAGCCATCCGGCGCATCGTGAAATGTGGCAAGCCCGCCGGCATCC
TGACGGGCGACGAGGCCTTTGCCCAGCGCTGCATCTCGCTGGGAACCCGCTTTACCGCGGTGGGCGTGGACGTGGGGCTG
TTGGCCCGGTCGACCTCGCAGCTGCGGCAGCGTTTCGGCGGCGCCAAAGCCTGA

Upstream 100 bases:

>100_bases
TTCAGCTAAGAGCCGCTCTAAACTCGCCGTCGGGTGCGCGAAATCCCTTCGCCGCCCGGTCGCAACAACGCGCGCAATCA
TCAAGCAACAAGGCGGAACC

Downstream 100 bases:

>100_bases
ATCAAGGCCTGACATCAGGCCTCGCGGTACAGCCCCGAATCGCCGCGCACGCCCAGCTTTTTCCGGAAGGCCTGCAGGTC
AAAGCCCTCGCGGGCGAGCT

Product: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Products: NA

Alternate protein names: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase; HHED aldolase; 4-hydroxy-2-ketoheptane-1,7-dioate aldolase; HKHD aldolase [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MELPVNQFKKMLLGGHQQIGLWCTLSSPYGVELLAGSGFEWLLLDTEHSPSDVTNVLAQLQTVAPYPVSAVVRPAWNDPV
LIKRYLDIGAQTLLIPYVQNAEEARRAVQSITYPGGGMRGVSALTRASRFGRVPNYAQTCRDELCLLVQIETLEALDNIE
AIAAVEGVDGIFIGPGDLAASMGHLGEPGNAAVVARIEEAIRRIVKCGKPAGILTGDEAFAQRCISLGTRFTAVGVDVGL
LARSTSQLRQRFGGAKA

Sequences:

>Translated_257_residues
MELPVNQFKKMLLGGHQQIGLWCTLSSPYGVELLAGSGFEWLLLDTEHSPSDVTNVLAQLQTVAPYPVSAVVRPAWNDPV
LIKRYLDIGAQTLLIPYVQNAEEARRAVQSITYPGGGMRGVSALTRASRFGRVPNYAQTCRDELCLLVQIETLEALDNIE
AIAAVEGVDGIFIGPGDLAASMGHLGEPGNAAVVARIEEAIRRIVKCGKPAGILTGDEAFAQRCISLGTRFTAVGVDVGL
LARSTSQLRQRFGGAKA
>Mature_257_residues
MELPVNQFKKMLLGGHQQIGLWCTLSSPYGVELLAGSGFEWLLLDTEHSPSDVTNVLAQLQTVAPYPVSAVVRPAWNDPV
LIKRYLDIGAQTLLIPYVQNAEEARRAVQSITYPGGGMRGVSALTRASRFGRVPNYAQTCRDELCLLVQIETLEALDNIE
AIAAVEGVDGIFIGPGDLAASMGHLGEPGNAAVVARIEEAIRRIVKCGKPAGILTGDEAFAQRCISLGTRFTAVGVDVGL
LARSTSQLRQRFGGAKA

Specific function: Catalyzes the reversible retro-aldol cleavage of 4- hydroxy-2-ketoheptane-1,7-dioate (HKHD) to pyruvate and succinic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788578, Length=252, Percent_Identity=47.6190476190476, Blast_Score=229, Evalue=1e-61,
Organism=Escherichia coli, GI1789514, Length=247, Percent_Identity=45.3441295546559, Blast_Score=220, Evalue=7e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR012689
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.2.-

Molecular weight: Translated: 27485; Mature: 27485

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELPVNQFKKMLLGGHQQIGLWCTLSSPYGVELLAGSGFEWLLLDTEHSPSDVTNVLAQL
CCCCHHHHHHHHHCCCCEEEEEEEECCCCCEEEEECCCCEEEEEECCCCHHHHHHHHHHH
QTVAPYPVSAVVRPAWNDPVLIKRYLDIGAQTLLIPYVQNAEEARRAVQSITYPGGGMRG
HHCCCCCHHHHCCCCCCCHHHHHHHHHCCCCEEEHHHHCCHHHHHHHHHHCCCCCCCHHH
VSALTRASRFGRVPNYAQTCRDELCLLVQIETLEALDNIEAIAAVEGVDGIFIGPGDLAA
HHHHHHHHHHCCCCCHHHHHHHHHEEEEEEHHHHHHHHHHHHHHHCCCCEEEECCHHHHH
SMGHLGEPGNAAVVARIEEAIRRIVKCGKPAGILTGDEAFAQRCISLGTRFTAVGVDVGL
HHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHCCCEEEECCHHHH
LARSTSQLRQRFGGAKA
HHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MELPVNQFKKMLLGGHQQIGLWCTLSSPYGVELLAGSGFEWLLLDTEHSPSDVTNVLAQL
CCCCHHHHHHHHHCCCCEEEEEEEECCCCCEEEEECCCCEEEEEECCCCHHHHHHHHHHH
QTVAPYPVSAVVRPAWNDPVLIKRYLDIGAQTLLIPYVQNAEEARRAVQSITYPGGGMRG
HHCCCCCHHHHCCCCCCCHHHHHHHHHCCCCEEEHHHHCCHHHHHHHHHHCCCCCCCHHH
VSALTRASRFGRVPNYAQTCRDELCLLVQIETLEALDNIEAIAAVEGVDGIFIGPGDLAA
HHHHHHHHHHCCCCCHHHHHHHHHEEEEEEHHHHHHHHHHHHHHHCCCCEEEECCHHHHH
SMGHLGEPGNAAVVARIEEAIRRIVKCGKPAGILTGDEAFAQRCISLGTRFTAVGVDVGL
HHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHCCCEEEECCHHHH
LARSTSQLRQRFGGAKA
HHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA