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The map label for this gene is dctA2 [H]

Identifier: 91787315

GI number: 91787315

Start: 1464315

End: 1465646

Strand: Reverse

Name: dctA2 [H]

Synonym: Bpro_1420

Alternate gene names: 91787315

Gene position: 1465646-1464315 (Counterclockwise)

Preceding gene: 91787316

Following gene: 91787314

Centisome position: 28.18

GC content: 64.79

Gene sequence:

>1332_bases
ATGCCCAAGTTTTTCCGCTCCCTGTTCGGTCAGGTGCTGCTGGCGCTGATCGTCGGCGTCGTCCTCGGTTTTGTCTGGCC
TGACACGGCCGTGCAGCTCAAGCCGCTCGGCGACGGCTTCATCAAGCTCATCAAGATGCTGATCCCGCTGATCGTGTTCT
GCGTGGTGGTGCACGGCATCGCCGGCACCGGCGACCTCAAGCGGGTCGGCCGGGTCGGCGTCAAGTCGCTGATCTACTTT
GAGGTCGTCACCACCGTCGCGCTGGCGCTGGGCCTGCTGCTGGCCTTCGTGTTCCAGCCCGGCGTGGGCATGAATGTCGA
CCCCAAGACGCTCGACGCCAGCGCCATGAGCGCCTACGCCACCAACGCGAGCAAGCTGACCGGCGGCGGCACCGTCGACT
TCCTGATGAAGCTGATCCCGACCACGGCGGTGAGCGCCTTTGCCAATGGCGATGTGCTGCAGGTGCTGCTGTTCGCTGTG
CTGTTCGGCTGCGCGCTGGCCATGCTGGGCGAGCGCGGCAAGCATGTCAGCAGTTTCATCGAAGACCTGTCCCATGTGCT
GTTCAAGACCATGGGCATCATCATCAAGCTGGCGCCCCTGGGCGTGCTGGGAGCCATCGCGTTCACCGTGGGCAAGTACG
GCATCGGCTCGCTCAAGCAGCTGGGCATGCTGGTGGTGCTGTTCTATGGCGCGGTGATCATCTTCGTGGTGGTCGTGCTC
GGCGCCATCATGCGCCTGTCGGGCTTCAACCTGTTCAAGCTGCTGCGCTACCTGCGCGAGGAACTGATGGTGGTGTTTGC
CACCACCTCGTCCGACAGCGTGCTGCCGCAGATCATGGCCAAGCTCAAGCACATGGGCATACGCGACTCCACGGTCGGCC
TGGTCATTCCTACCGGCTATTCGTTCAACCTCGACGCATTCTCGATCTACATCACGCTGGCGGCCGTCTTCATTGCCCAG
GCCACGAACACACCAATCTCGATGGCCGACCTGCTGACCATCCTGGCGATCTCGCTGGTCACCTCCAAGGGCGCCCATGG
TGTGCCGGGCTCGGCGATCGTGGTGCTGGCGGCCACCCTGCAGGCGATTCCGGCCATACCCGCCATCGGCCTGGTGCTGG
TGCTGTCGGTGGACTGGTTCATGGGCATCGCCCGCGCCCTGGGCAACCTGATCGGCAACTGCGTGGCCACGGTCGCGGTG
GCCGCCTGGGAGGGCGACATCGACCGCGAGCGGGCGCATGCCGTTCTCAATGGTGAACCCCATGCGGTGTTCGACATCGA
GGACGAGGAGCCCGCGCCGGCGTCCGTGCCGTTGGCCGCGCCAGTCCGCTAA

Upstream 100 bases:

>100_bases
AACCCTGAAATGTTATTTCTCCAGTTTGCCTACATTACTTGTATGCAAGACTTGTGAGCAATCGGCCTTGCTGTTCAGAA
ACCCTCTTTCGGAGACTGCC

Downstream 100 bases:

>100_bases
AGTCCATGCCATGAGCAGCCAGAACCAGATTTCCCAGCAGGTGGTCGAATCCATCCTCGCGCAGAAGCTCGCCCCCGGCG
AGCGCCTGGGCGAGCAGGAG

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MPKFFRSLFGQVLLALIVGVVLGFVWPDTAVQLKPLGDGFIKLIKMLIPLIVFCVVVHGIAGTGDLKRVGRVGVKSLIYF
EVVTTVALALGLLLAFVFQPGVGMNVDPKTLDASAMSAYATNASKLTGGGTVDFLMKLIPTTAVSAFANGDVLQVLLFAV
LFGCALAMLGERGKHVSSFIEDLSHVLFKTMGIIIKLAPLGVLGAIAFTVGKYGIGSLKQLGMLVVLFYGAVIIFVVVVL
GAIMRLSGFNLFKLLRYLREELMVVFATTSSDSVLPQIMAKLKHMGIRDSTVGLVIPTGYSFNLDAFSIYITLAAVFIAQ
ATNTPISMADLLTILAISLVTSKGAHGVPGSAIVVLAATLQAIPAIPAIGLVLVLSVDWFMGIARALGNLIGNCVATVAV
AAWEGDIDRERAHAVLNGEPHAVFDIEDEEPAPASVPLAAPVR

Sequences:

>Translated_443_residues
MPKFFRSLFGQVLLALIVGVVLGFVWPDTAVQLKPLGDGFIKLIKMLIPLIVFCVVVHGIAGTGDLKRVGRVGVKSLIYF
EVVTTVALALGLLLAFVFQPGVGMNVDPKTLDASAMSAYATNASKLTGGGTVDFLMKLIPTTAVSAFANGDVLQVLLFAV
LFGCALAMLGERGKHVSSFIEDLSHVLFKTMGIIIKLAPLGVLGAIAFTVGKYGIGSLKQLGMLVVLFYGAVIIFVVVVL
GAIMRLSGFNLFKLLRYLREELMVVFATTSSDSVLPQIMAKLKHMGIRDSTVGLVIPTGYSFNLDAFSIYITLAAVFIAQ
ATNTPISMADLLTILAISLVTSKGAHGVPGSAIVVLAATLQAIPAIPAIGLVLVLSVDWFMGIARALGNLIGNCVATVAV
AAWEGDIDRERAHAVLNGEPHAVFDIEDEEPAPASVPLAAPVR
>Mature_442_residues
PKFFRSLFGQVLLALIVGVVLGFVWPDTAVQLKPLGDGFIKLIKMLIPLIVFCVVVHGIAGTGDLKRVGRVGVKSLIYFE
VVTTVALALGLLLAFVFQPGVGMNVDPKTLDASAMSAYATNASKLTGGGTVDFLMKLIPTTAVSAFANGDVLQVLLFAVL
FGCALAMLGERGKHVSSFIEDLSHVLFKTMGIIIKLAPLGVLGAIAFTVGKYGIGSLKQLGMLVVLFYGAVIIFVVVVLG
AIMRLSGFNLFKLLRYLREELMVVFATTSSDSVLPQIMAKLKHMGIRDSTVGLVIPTGYSFNLDAFSIYITLAAVFIAQA
TNTPISMADLLTILAISLVTSKGAHGVPGSAIVVLAATLQAIPAIPAIGLVLVLSVDWFMGIARALGNLIGNCVATVAVA
AWEGDIDRERAHAVLNGEPHAVFDIEDEEPAPASVPLAAPVR

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI4827012, Length=497, Percent_Identity=24.1448692152917, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI21314632, Length=399, Percent_Identity=27.8195488721804, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI169790839, Length=451, Percent_Identity=24.1685144124169, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI66773030, Length=375, Percent_Identity=24.5333333333333, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI194239697, Length=455, Percent_Identity=23.0769230769231, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI40254478, Length=458, Percent_Identity=24.0174672489083, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI5032093, Length=424, Percent_Identity=25.9433962264151, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI223468564, Length=297, Percent_Identity=25.2525252525253, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI223468566, Length=297, Percent_Identity=25.2525252525253, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI262359914, Length=389, Percent_Identity=23.9074550128535, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI301601644, Length=165, Percent_Identity=29.6969696969697, Blast_Score=76, Evalue=6e-14,
Organism=Escherichia coli, GI1789947, Length=415, Percent_Identity=53.0120481927711, Blast_Score=445, Evalue=1e-126,
Organism=Escherichia coli, GI1790514, Length=419, Percent_Identity=40.3341288782816, Blast_Score=282, Evalue=4e-77,
Organism=Escherichia coli, GI1789473, Length=329, Percent_Identity=22.7963525835866, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788024, Length=418, Percent_Identity=21.7703349282297, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17537407, Length=460, Percent_Identity=25.4347826086956, Blast_Score=115, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI71996953, Length=482, Percent_Identity=23.4439834024896, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI193206505, Length=434, Percent_Identity=23.0414746543779, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI193206654, Length=448, Percent_Identity=20.9821428571429, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI71983106, Length=480, Percent_Identity=22.7083333333333, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71983099, Length=477, Percent_Identity=22.0125786163522, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17541374, Length=401, Percent_Identity=22.4438902743142, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24583025, Length=427, Percent_Identity=23.8875878220141, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI17137668, Length=427, Percent_Identity=23.8875878220141, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24583023, Length=427, Percent_Identity=23.8875878220141, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI281360483, Length=464, Percent_Identity=25.6465517241379, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI17137666, Length=464, Percent_Identity=25.6465517241379, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI281360481, Length=464, Percent_Identity=25.6465517241379, Blast_Score=89, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 46659; Mature: 46528

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKFFRSLFGQVLLALIVGVVLGFVWPDTAVQLKPLGDGFIKLIKMLIPLIVFCVVVHGI
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCC
AGTGDLKRVGRVGVKSLIYFEVVTTVALALGLLLAFVFQPGVGMNVDPKTLDASAMSAYA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
TNASKLTGGGTVDFLMKLIPTTAVSAFANGDVLQVLLFAVLFGCALAMLGERGKHVSSFI
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
EDLSHVLFKTMGIIIKLAPLGVLGAIAFTVGKYGIGSLKQLGMLVVLFYGAVIIFVVVVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GAIMRLSGFNLFKLLRYLREELMVVFATTSSDSVLPQIMAKLKHMGIRDSTVGLVIPTGY
HHHHHHCCCCHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEECCC
SFNLDAFSIYITLAAVFIAQATNTPISMADLLTILAISLVTSKGAHGVPGSAIVVLAATL
CCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
QAIPAIPAIGLVLVLSVDWFMGIARALGNLIGNCVATVAVAAWEGDIDRERAHAVLNGEP
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCC
HAVFDIEDEEPAPASVPLAAPVR
CEEEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PKFFRSLFGQVLLALIVGVVLGFVWPDTAVQLKPLGDGFIKLIKMLIPLIVFCVVVHGI
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCC
AGTGDLKRVGRVGVKSLIYFEVVTTVALALGLLLAFVFQPGVGMNVDPKTLDASAMSAYA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
TNASKLTGGGTVDFLMKLIPTTAVSAFANGDVLQVLLFAVLFGCALAMLGERGKHVSSFI
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
EDLSHVLFKTMGIIIKLAPLGVLGAIAFTVGKYGIGSLKQLGMLVVLFYGAVIIFVVVVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GAIMRLSGFNLFKLLRYLREELMVVFATTSSDSVLPQIMAKLKHMGIRDSTVGLVIPTGY
HHHHHHCCCCHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEECCC
SFNLDAFSIYITLAAVFIAQATNTPISMADLLTILAISLVTSKGAHGVPGSAIVVLAATL
CCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
QAIPAIPAIGLVLVLSVDWFMGIARALGNLIGNCVATVAVAAWEGDIDRERAHAVLNGEP
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCC
HAVFDIEDEEPAPASVPLAAPVR
CEEEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]