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The map label for this gene is rhtB [H]

Identifier: 91787173

GI number: 91787173

Start: 1318942

End: 1319580

Strand: Reverse

Name: rhtB [H]

Synonym: Bpro_1277

Alternate gene names: 91787173

Gene position: 1319580-1318942 (Counterclockwise)

Preceding gene: 91787174

Following gene: 91787172

Centisome position: 25.38

GC content: 67.61

Gene sequence:

>639_bases
ATGCTTCCCGACACCCCGTTGCTCCTGGCTTTCGTGGGTGCCAGCCTGGTGCTGGCTCTCACGCCCGGCCCCGCCGTCGT
CTATATCGTGGCCCGCACTCTGGCACAGGGTCGCTCCTGCGGTCTCGCTTCCGTGCTGGGCGTGGCGCTGGGCAACCTCT
CCAACGCCGTGGGCGCCGCGCTCGGGCTGGCCGCTTTGTTCGCTGTTTCATCGGTCGCATTCACCGTGGTGAAATGGGCC
GGGGCCGCCTACCTCGTATACCTCGGCATCCGGATGTGGCGGGCGGTGCCGGCCATCGCAGCTGCGGCGTTACAAGCACC
GGTGCAGCCCCTGCGGCGTGTCTTTCGCGACGGCTTCATGGTGGCGCTATTGAACCCGAAAACCACCTTGTTCTTCGCCG
CCTTTCTGCCGCAATTCATGGACGCGCACGCCAGCCCGCTGATGCAGACCCTGGCGCTCGGCGGGGTCTTCATCGGTATC
GCAGGCTGCACTGACCTCATCTACGTGCTGACCGCAAGCCTCGTCGCACCGCGCCTGAGCCGGGCGGCCCGGCACGCCGT
CTGGGGCAACCGGCTCGCGGGTACGTCTTTCATCGCCTTGGGTGCGCTCACGGCCATGGGCACGCGTCCCACGCGCTAA

Upstream 100 bases:

>100_bases
ACTCTTGCCGTGCATGTCCAACCTTGTGGCCCGGCCAGATGACTCTTTACAAGCAGCGATGGCGCAGCGACACTTGTTGC
TCCAAGAATCCGGAGTTCAT

Downstream 100 bases:

>100_bases
CCATTGCCGCGTCGCCGATGCGGTCTCGCTAGAAATCATCAGCCAGGCGGGCGGCGACCCGGCGGCGGTGAGCTTTGACG
CCGTGACCGCCGGCAAGGCA

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MLPDTPLLLAFVGASLVLALTPGPAVVYIVARTLAQGRSCGLASVLGVALGNLSNAVGAALGLAALFAVSSVAFTVVKWA
GAAYLVYLGIRMWRAVPAIAAAALQAPVQPLRRVFRDGFMVALLNPKTTLFFAAFLPQFMDAHASPLMQTLALGGVFIGI
AGCTDLIYVLTASLVAPRLSRAARHAVWGNRLAGTSFIALGALTAMGTRPTR

Sequences:

>Translated_212_residues
MLPDTPLLLAFVGASLVLALTPGPAVVYIVARTLAQGRSCGLASVLGVALGNLSNAVGAALGLAALFAVSSVAFTVVKWA
GAAYLVYLGIRMWRAVPAIAAAALQAPVQPLRRVFRDGFMVALLNPKTTLFFAAFLPQFMDAHASPLMQTLALGGVFIGI
AGCTDLIYVLTASLVAPRLSRAARHAVWGNRLAGTSFIALGALTAMGTRPTR
>Mature_212_residues
MLPDTPLLLAFVGASLVLALTPGPAVVYIVARTLAQGRSCGLASVLGVALGNLSNAVGAALGLAALFAVSSVAFTVVKWA
GAAYLVYLGIRMWRAVPAIAAAALQAPVQPLRRVFRDGFMVALLNPKTTLFFAAFLPQFMDAHASPLMQTLALGGVFIGI
AGCTDLIYVLTASLVAPRLSRAARHAVWGNRLAGTSFIALGALTAMGTRPTR

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=200, Percent_Identity=35, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1788099, Length=192, Percent_Identity=25, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786522, Length=146, Percent_Identity=29.4520547945205, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 21917; Mature: 21917

Theoretical pI: Translated: 11.82; Mature: 11.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPDTPLLLAFVGASLVLALTPGPAVVYIVARTLAQGRSCGLASVLGVALGNLSNAVGAA
CCCCHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
LGLAALFAVSSVAFTVVKWAGAAYLVYLGIRMWRAVPAIAAAALQAPVQPLRRVFRDGFM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
VALLNPKTTLFFAAFLPQFMDAHASPLMQTLALGGVFIGIAGCTDLIYVLTASLVAPRLS
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAARHAVWGNRLAGTSFIALGALTAMGTRPTR
HHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLPDTPLLLAFVGASLVLALTPGPAVVYIVARTLAQGRSCGLASVLGVALGNLSNAVGAA
CCCCHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
LGLAALFAVSSVAFTVVKWAGAAYLVYLGIRMWRAVPAIAAAALQAPVQPLRRVFRDGFM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
VALLNPKTTLFFAAFLPQFMDAHASPLMQTLALGGVFIGIAGCTDLIYVLTASLVAPRLS
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAARHAVWGNRLAGTSFIALGALTAMGTRPTR
HHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]