Click here to switch to the map view.

The map label for this gene is rpoH [H]

Identifier: 91787153

GI number: 91787153

Start: 1300095

End: 1301063

Strand: Reverse

Name: rpoH [H]

Synonym: Bpro_1256

Alternate gene names: 91787153

Gene position: 1301063-1300095 (Counterclockwise)

Preceding gene: 91787160

Following gene: 91787147

Centisome position: 25.02

GC content: 60.89

Gene sequence:

>969_bases
GTGTCATACGCTCTTTCCCCCCAGGCCCTGACGGGCGCCCTTGCTGTACCCGTGCCCCGCGCCGGTGCTGTGGCCACCGT
CAACCCCTGGTCGCTGGTACCTCCGCTGGGCAACCTGGATGCCTACATTTCGGCGGTCAACCGTCTGCCCATGCTCACTT
TGGAGCAGGAGCAGGAATTTGCCAAAAAGCTCAAAGAAAACAACGATCTCGACTCGGCCGGTAAGTTAGTGCTCTCTCAC
TTACGCCTGGTGGTGTCGATTTCCCGCAAATACCTGGGTTACGGGCTGCCGCATGGCGACCTGATCCAGGAGGGCAATAT
CGGCCTGATGAAGGCGGTCAAGCGCTTTGACCCGGAACAGAACGTGCGGCTGGTGAGCTATGCCATGCACTGGATCAAGG
CCGAGATTCACGAGTACATCCTGAAAAACTGGCGCATGGTCAAGGTGGCCACCACCAAAGCCCAGCGCAAGCTGTTCTTC
AACCTGCGCTCGATGAAACAGGGCTTCAAGGACGACGCCGATGTGGCGACGCACCGCGACACGCTGACCGAAGGCCAGAT
TGACTCGATGGCCAAAACACTGAACGTCAAGCGCGAGGAAGTCATCGAGATGGAGCAGCGCATGTCGGGCGGCGACGTGC
TGCTGGACCCGAGCCCGAGCGACGACGGCGAAGACGCCTTTGGGCCGATTGCCTACCTGGCCGATGCCACGCAAGAGCCG
ACCGCCCTGATGGAATCCCGCCAGCGCGACAGCCTGGCTTCAGACGGCATCAGCGCCGCGCTGCAAGAGCTGGATCCACG
TTCGCGCCGCATTGTGGAAGAGCGCTGGCTCAAGGTCAACGACGATGGCTCGGGTGGCATGACGCTGCACGACCTGGCGG
ACGAGTACCAGGTCAGCGCCGAACGGATTCGCCAGATTGAAGTCTCGGCAATGAAGAAAATGAAAAAGGTGCTGGCGGCC
TACGCCTGA

Upstream 100 bases:

>100_bases
GCACGTATTAGCCTTGCGCTATCCGCGGCATTGAACCTATTGCTTAGCACTCTCTCCAATGGAGTGCTAATATTTAAAAC
TAGAGAAAAGGAGTTCTGCT

Downstream 100 bases:

>100_bases
GCCGGCGATCTGCTGAGCCACCCTGGCACGCTGACGTGCAACCAGGCCCGCTCCCTTTAACAAGGTGCGGGCCTTTTTTA
TGGCCGCCACGTGGCGCTCT

Product: sigma 32 (RpoH)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MSYALSPQALTGALAVPVPRAGAVATVNPWSLVPPLGNLDAYISAVNRLPMLTLEQEQEFAKKLKENNDLDSAGKLVLSH
LRLVVSISRKYLGYGLPHGDLIQEGNIGLMKAVKRFDPEQNVRLVSYAMHWIKAEIHEYILKNWRMVKVATTKAQRKLFF
NLRSMKQGFKDDADVATHRDTLTEGQIDSMAKTLNVKREEVIEMEQRMSGGDVLLDPSPSDDGEDAFGPIAYLADATQEP
TALMESRQRDSLASDGISAALQELDPRSRRIVEERWLKVNDDGSGGMTLHDLADEYQVSAERIRQIEVSAMKKMKKVLAA
YA

Sequences:

>Translated_322_residues
MSYALSPQALTGALAVPVPRAGAVATVNPWSLVPPLGNLDAYISAVNRLPMLTLEQEQEFAKKLKENNDLDSAGKLVLSH
LRLVVSISRKYLGYGLPHGDLIQEGNIGLMKAVKRFDPEQNVRLVSYAMHWIKAEIHEYILKNWRMVKVATTKAQRKLFF
NLRSMKQGFKDDADVATHRDTLTEGQIDSMAKTLNVKREEVIEMEQRMSGGDVLLDPSPSDDGEDAFGPIAYLADATQEP
TALMESRQRDSLASDGISAALQELDPRSRRIVEERWLKVNDDGSGGMTLHDLADEYQVSAERIRQIEVSAMKKMKKVLAA
YA
>Mature_321_residues
SYALSPQALTGALAVPVPRAGAVATVNPWSLVPPLGNLDAYISAVNRLPMLTLEQEQEFAKKLKENNDLDSAGKLVLSHL
RLVVSISRKYLGYGLPHGDLIQEGNIGLMKAVKRFDPEQNVRLVSYAMHWIKAEIHEYILKNWRMVKVATTKAQRKLFFN
LRSMKQGFKDDADVATHRDTLTEGQIDSMAKTLNVKREEVIEMEQRMSGGDVLLDPSPSDDGEDAFGPIAYLADATQEPT
ALMESRQRDSLASDGISAALQELDPRSRRIVEERWLKVNDDGSGGMTLHDLADEYQVSAERIRQIEVSAMKKMKKVLAAY
A

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=289, Percent_Identity=48.7889273356401, Blast_Score=266, Evalue=1e-72,
Organism=Escherichia coli, GI1789098, Length=277, Percent_Identity=29.2418772563177, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI1789448, Length=312, Percent_Identity=28.8461538461538, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 35851; Mature: 35720

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYALSPQALTGALAVPVPRAGAVATVNPWSLVPPLGNLDAYISAVNRLPMLTLEQEQEF
CCCCCCCHHHHCHHCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHCCCEEEHHHHHH
AKKLKENNDLDSAGKLVLSHLRLVVSISRKYLGYGLPHGDLIQEGNIGLMKAVKRFDPEQ
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCHHHHHHHHHCCCCC
NVRLVSYAMHWIKAEIHEYILKNWRMVKVATTKAQRKLFFNLRSMKQGFKDDADVATHRD
CHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
TLTEGQIDSMAKTLNVKREEVIEMEQRMSGGDVLLDPSPSDDGEDAFGPIAYLADATQEP
HHCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHCCCCCH
TALMESRQRDSLASDGISAALQELDPRSRRIVEERWLKVNDDGSGGMTLHDLADEYQVSA
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCEEECCCCCCCCCHHHHHHHHHHHH
ERIRQIEVSAMKKMKKVLAAYA
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SYALSPQALTGALAVPVPRAGAVATVNPWSLVPPLGNLDAYISAVNRLPMLTLEQEQEF
CCCCCCHHHHCHHCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHCCCEEEHHHHHH
AKKLKENNDLDSAGKLVLSHLRLVVSISRKYLGYGLPHGDLIQEGNIGLMKAVKRFDPEQ
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCHHHHHHHHHCCCCC
NVRLVSYAMHWIKAEIHEYILKNWRMVKVATTKAQRKLFFNLRSMKQGFKDDADVATHRD
CHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
TLTEGQIDSMAKTLNVKREEVIEMEQRMSGGDVLLDPSPSDDGEDAFGPIAYLADATQEP
HHCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHCCCCCH
TALMESRQRDSLASDGISAALQELDPRSRRIVEERWLKVNDDGSGGMTLHDLADEYQVSA
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCEEECCCCCCCCCHHHHHHHHHHHH
ERIRQIEVSAMKKMKKVLAAYA
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]