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The map label for this gene is yfhA [H]

Identifier: 91787015

GI number: 91787015

Start: 1153679

End: 1155079

Strand: Reverse

Name: yfhA [H]

Synonym: Bpro_1117

Alternate gene names: 91787015

Gene position: 1155079-1153679 (Counterclockwise)

Preceding gene: 91787016

Following gene: 91787014

Centisome position: 22.21

GC content: 66.52

Gene sequence:

>1401_bases
ATGAGTGACTCCTCGACGAGTTCCGCCGCGACGGCGACCCCGTCGGCCCATCTGCTGGTCGTTGACGACGACCCGGACAT
GCTGCGCCTGCTGACGATACGGCTCACCGCGGCGGGTTACCGCGTGACGGCGGTCACCTCTGCAGAGGCTGCCATGACCC
AGCTCCATATCGAGCGGCCGCAGCTGGTGCTGAGCGACGTGCAGCTTCCCGGCCGGGATGGCCTGGCCCTGTTTGACGAC
ATCCGCGCCCGGCATCCGTCGCTGCCGGTGATCCTGCTGACGGCGCATGGCACCATTCCCGATGCGGTGGAGGCGACCGA
GCGAGGTGTCTTCACCTACCTGACCAAACCTTTTGATGGCAAGGGCCTGCTCGACAAGATCGCCCAGGCGCTGGCGCTGA
GCGCGCCTTCGCATCCGGCCAAGCGGGGCAGCCCCGAGGCATGGCAACACCAGATCATCAGCCGCTCCAGCCGCATGGCC
GAAGCGCTGGCGGAAGCGCGCATGATCTCGCAATCGGATGCCAGTGTGCTGCTGCGCGGTGAAAGCGGCACCGGCAAGGA
ACTGCTGGCCCAGGCCATCCACCAGGCCAGCCCGCGCGCCGGCAAACCCTTTATTGCCGTCAACTGCGGCGCCATCCCCG
AGGCCCTGCTGGAGTCGGAACTGTTCGGCCACGTCAAGGGCGCCTTCACCGACGCGGTGGCCAGTCACAAGGGCTTGTTC
CAGGCGGCCGATGGCGGCACCTTGTTGCTTGACGAGATTGGCGACATGCCGCCGGCCCTCCAGGTCAAGCTGCTGCGCGT
GCTGCAGGAGCGGGTGGTGCGGCCCCTGGGCTCAAGCCAGTCCATCCCGGTGGACGTGCGCATCATCTCGGCCACGCACC
GCGACCTGGACGCCGCCATGGCCGAGGGCCAGTTCCGCGCCGATCTGTATTACCGCCTCAATGTGGTGACGCTGACGCTG
CCGCCGCTGTCGGAGCGCCGCGAAGACATTCCCTTGCTGGCCAACCATTTCCTCATCAAGCTGGCCAACAAGTACGACAA
GCGCCTGAGCGGCTTCGCCCCGGAAGCCATCAAGGCCCTGACCACGGCCGCCTGGCCAGGCAATGTGCGACAGCTCTACA
ACGTGGTGGAGCAGGTCTGTGCGCTGTCGACGACGCCGCTCGTGCCACTGGCCCTGGTGCAGCGCGCCCTGCGCTCGCCC
AGTGTGCAGGTACTTACTTTTGCAGAGGCCAGGCAACGTTTTGAGCGCGACTACCTGGTCGGCCTGCTCAAACTGACCGA
TGGCAATGTGGCCGACGCCGCGCGCCTGGCCGACCGTAACCGCACCGAGTTTTACCGCCTGATGCAAAAACACGGCCTGA
CCCCCGGGCATTTCAAGGCCGACAACGCCACCGCTGCCTGA

Upstream 100 bases:

>100_bases
TGAACGCAGCCTGGCAAGCCGCCCCCCGGCTGCGCCCGCATCGTCCCCGGCACCGGCCACCAACCGCGGCCGGTCTGCGG
CGCCGTGAAAGAGGGACGCC

Downstream 100 bases:

>100_bases
CGGCTGGCGCAGGCCGCGCCGCCGGGCAGTCGCTGGCTGGCGACAGCCCTGGAGGCCTGATTTCAAAGGGATTTTTCTTC
CAGACCCAGCGGGCTGTCGC

Product: Fis family two component sigma-54 specific transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MSDSSTSSAATATPSAHLLVVDDDPDMLRLLTIRLTAAGYRVTAVTSAEAAMTQLHIERPQLVLSDVQLPGRDGLALFDD
IRARHPSLPVILLTAHGTIPDAVEATERGVFTYLTKPFDGKGLLDKIAQALALSAPSHPAKRGSPEAWQHQIISRSSRMA
EALAEARMISQSDASVLLRGESGTGKELLAQAIHQASPRAGKPFIAVNCGAIPEALLESELFGHVKGAFTDAVASHKGLF
QAADGGTLLLDEIGDMPPALQVKLLRVLQERVVRPLGSSQSIPVDVRIISATHRDLDAAMAEGQFRADLYYRLNVVTLTL
PPLSERREDIPLLANHFLIKLANKYDKRLSGFAPEAIKALTTAAWPGNVRQLYNVVEQVCALSTTPLVPLALVQRALRSP
SVQVLTFAEARQRFERDYLVGLLKLTDGNVADAARLADRNRTEFYRLMQKHGLTPGHFKADNATAA

Sequences:

>Translated_466_residues
MSDSSTSSAATATPSAHLLVVDDDPDMLRLLTIRLTAAGYRVTAVTSAEAAMTQLHIERPQLVLSDVQLPGRDGLALFDD
IRARHPSLPVILLTAHGTIPDAVEATERGVFTYLTKPFDGKGLLDKIAQALALSAPSHPAKRGSPEAWQHQIISRSSRMA
EALAEARMISQSDASVLLRGESGTGKELLAQAIHQASPRAGKPFIAVNCGAIPEALLESELFGHVKGAFTDAVASHKGLF
QAADGGTLLLDEIGDMPPALQVKLLRVLQERVVRPLGSSQSIPVDVRIISATHRDLDAAMAEGQFRADLYYRLNVVTLTL
PPLSERREDIPLLANHFLIKLANKYDKRLSGFAPEAIKALTTAAWPGNVRQLYNVVEQVCALSTTPLVPLALVQRALRSP
SVQVLTFAEARQRFERDYLVGLLKLTDGNVADAARLADRNRTEFYRLMQKHGLTPGHFKADNATAA
>Mature_465_residues
SDSSTSSAATATPSAHLLVVDDDPDMLRLLTIRLTAAGYRVTAVTSAEAAMTQLHIERPQLVLSDVQLPGRDGLALFDDI
RARHPSLPVILLTAHGTIPDAVEATERGVFTYLTKPFDGKGLLDKIAQALALSAPSHPAKRGSPEAWQHQIISRSSRMAE
ALAEARMISQSDASVLLRGESGTGKELLAQAIHQASPRAGKPFIAVNCGAIPEALLESELFGHVKGAFTDAVASHKGLFQ
AADGGTLLLDEIGDMPPALQVKLLRVLQERVVRPLGSSQSIPVDVRIISATHRDLDAAMAEGQFRADLYYRLNVVTLTLP
PLSERREDIPLLANHFLIKLANKYDKRLSGFAPEAIKALTTAAWPGNVRQLYNVVEQVCALSTTPLVPLALVQRALRSPS
VQVLTFAEARQRFERDYLVGLLKLTDGNVADAARLADRNRTEFYRLMQKHGLTPGHFKADNATAA

Specific function: Probable member of a two-component regulatory system yfhA/yfhK [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788905, Length=444, Percent_Identity=57.2072072072072, Blast_Score=503, Evalue=1e-144,
Organism=Escherichia coli, GI1790437, Length=426, Percent_Identity=44.131455399061, Blast_Score=339, Evalue=2e-94,
Organism=Escherichia coli, GI1788550, Length=457, Percent_Identity=40.4814004376368, Blast_Score=338, Evalue=6e-94,
Organism=Escherichia coli, GI1790299, Length=470, Percent_Identity=42.5531914893617, Blast_Score=322, Evalue=3e-89,
Organism=Escherichia coli, GI87082117, Length=320, Percent_Identity=47.8125, Blast_Score=278, Evalue=4e-76,
Organism=Escherichia coli, GI1789087, Length=309, Percent_Identity=44.336569579288, Blast_Score=251, Evalue=9e-68,
Organism=Escherichia coli, GI1789233, Length=215, Percent_Identity=56.7441860465116, Blast_Score=241, Evalue=7e-65,
Organism=Escherichia coli, GI87082152, Length=319, Percent_Identity=39.4984326018809, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI1786524, Length=323, Percent_Identity=40.5572755417957, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1787583, Length=314, Percent_Identity=39.171974522293, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI87081872, Length=244, Percent_Identity=43.4426229508197, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI87081858, Length=300, Percent_Identity=33.6666666666667, Blast_Score=165, Evalue=5e-42,
Organism=Escherichia coli, GI1789828, Length=248, Percent_Identity=37.9032258064516, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1789809, Length=177, Percent_Identity=30.5084745762712, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1790552, Length=101, Percent_Identity=36.6336633663366, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI145693157, Length=140, Percent_Identity=34.2857142857143, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788394, Length=133, Percent_Identity=27.8195488721804, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50403; Mature: 50272

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSSTSSAATATPSAHLLVVDDDPDMLRLLTIRLTAAGYRVTAVTSAEAAMTQLHIERP
CCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHEEEECCEEEEEECCHHHHHHHHHCCCC
QLVLSDVQLPGRDGLALFDDIRARHPSLPVILLTAHGTIPDAVEATERGVFTYLTKPFDG
HHEEECCCCCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHCCEEEEECCCCCC
KGLLDKIAQALALSAPSHPAKRGSPEAWQHQIISRSSRMAEALAEARMISQSDASVLLRG
CCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
ESGTGKELLAQAIHQASPRAGKPFIAVNCGAIPEALLESELFGHVKGAFTDAVASHKGLF
CCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCE
QAADGGTLLLDEIGDMPPALQVKLLRVLQERVVRPLGSSQSIPVDVRIISATHRDLDAAM
EECCCCEEEHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECHHHHHHHHH
AEGQFRADLYYRLNVVTLTLPPLSERREDIPLLANHFLIKLANKYDKRLSGFAPEAIKAL
HCCCEEEEEEEEEEEEEEECCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
TTAAWPGNVRQLYNVVEQVCALSTTPLVPLALVQRALRSPSVQVLTFAEARQRFERDYLV
HHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHE
GLLKLTDGNVADAARLADRNRTEFYRLMQKHGLTPGHFKADNATAA
EEEEECCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCEECCCCCCC
>Mature Secondary Structure 
SDSSTSSAATATPSAHLLVVDDDPDMLRLLTIRLTAAGYRVTAVTSAEAAMTQLHIERP
CCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHEEEECCEEEEEECCHHHHHHHHHCCCC
QLVLSDVQLPGRDGLALFDDIRARHPSLPVILLTAHGTIPDAVEATERGVFTYLTKPFDG
HHEEECCCCCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHCCEEEEECCCCCC
KGLLDKIAQALALSAPSHPAKRGSPEAWQHQIISRSSRMAEALAEARMISQSDASVLLRG
CCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
ESGTGKELLAQAIHQASPRAGKPFIAVNCGAIPEALLESELFGHVKGAFTDAVASHKGLF
CCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCE
QAADGGTLLLDEIGDMPPALQVKLLRVLQERVVRPLGSSQSIPVDVRIISATHRDLDAAM
EECCCCEEEHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECHHHHHHHHH
AEGQFRADLYYRLNVVTLTLPPLSERREDIPLLANHFLIKLANKYDKRLSGFAPEAIKAL
HCCCEEEEEEEEEEEEEEECCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
TTAAWPGNVRQLYNVVEQVCALSTTPLVPLALVQRALRSPSVQVLTFAEARQRFERDYLV
HHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHE
GLLKLTDGNVADAARLADRNRTEFYRLMQKHGLTPGHFKADNATAA
EEEEECCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]