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The map label for this gene is pilS [H]

Identifier: 91787009

GI number: 91787009

Start: 1145546

End: 1147261

Strand: Reverse

Name: pilS [H]

Synonym: Bpro_1111

Alternate gene names: 91787009

Gene position: 1147261-1145546 (Counterclockwise)

Preceding gene: 91787010

Following gene: 91787008

Centisome position: 22.06

GC content: 64.63

Gene sequence:

>1716_bases
ATGACGACACCCTCCCAATGGTTTGAGACGGCGGAAAAGGCCTCCGGAGCCTGGGAGTTCGAACCTGAATTCAGCTCCTT
CGGCCGCCTCTGGCGCGTTTTCATGACGGCGCGTGTCGCCACGGCCGGCGTGCTGGTGGTCCTGCAGGCTGTCATCCACG
CCTTGGGCAACAGCAGCAACCGCTGGCCCGTCGCCGTGTGCATCGCCTACCTGTGTGCGACGCTGGCCGTGCGGCTTTGG
GCCCAGCCCCGGCCACCGGGCCGCACGTTTGACGCACACTGGCTGGCCACCATCGGGGTGGACGTGATCACCTTTTCCCT
GCTGGAATTCCTGCAATCGGGCGGCATCAATTACACCCCGCTGTTCGCACTGCCGGTGCTTCTGTCGTCGATTCTCGGGC
CGATTCTGCTGGCCTTCGGCACGGCTGCCAGCGTCACGCTGTTGCTGCTCGTCGATGCCTGGTGGACTTCGCTGCAAACC
CCGGGGGACTTCAATGCGCGCTTTCTTCAGGCCGGCCTGAGCGGCTCGGGGTTCTTCCTGGTGGCGCTGCTGGCCAACCA
GCTGGCGCTGCGCCTGGCGCGGGAAGAGCAACGCGCCAAAGCCAGCCAGTCAGCGGCCCGCATGCAGACCCAGGTCAACG
AACTGGTCATTGAAGCATTGACCGACGGCGTGCTGGTGGTTGACGTCAATGGCGTCGTGCGCTCGGCGAACCCCGCCTCC
AGGCGGCTGCTGTTCATGCGCAAGGCCATTCGCAACGCGCCGTTCATGCTGGCCACGGAGAGCGCCTGGCAACCCCTTGC
GGACCTGATGCACCAGACATTTGCCACGCAATCGCCCCAGGAAGCGGACATCCGGCTCGAATTTCCAGCCCGCAGCGCAA
GGCGGCTGCATGTGCGCACCCGGCTGGCGGCCTCGCAAAACGGCACGCACGAAAGCCTGTGCGTGATGTTTCTCGAGGAC
CTGCGTGAAATGGAAGCACGTGTGCGTACCGAAAAAATGGCAGCCATGGGGCGCATGTCCGCTGCGGTGGCCCACGAAAT
CAGGAATCCGCTGGCGGCCATTTCGCAGGCCAACGCCCTGCTGGAAGAAGATCTGCAGGATGCCGGCCACCGCCAGCTCA
CCGCGCTGGTCAGCCAAAACGCGCAGCGCCTGGCCAAAATTGTCGACGAGGTCCTGAACATCTCGCGGGCCCAGGAACAG
GCACCCGCGCCGCAAACGACGATGCTGATGCTGGACGACACGGTTGCTCAAATCGCCACCGACTGGGCCAGCCAGACCGG
GGCAGCGCAGCGCTTGGGCGTAACCCTGAACGCGGCCCAGACATCGGTCTGGTTCGAGGCCGAGCACCTGCGGCGCCTGA
TGGTCAACCTGCTGGATAACGCCCTGCGTTACGCCAGCGCGTCCGCGCACTCGATGGAAGTAGCCACCCGGGTGATGACG
GGAGGCGAGGTCCGGCTGGCGGTCTGGAGTGACGGCCAACCGCTGGAAGCAACGGTCGAGACCCATTTGTTCGAACCTTT
TTTCTCCTCCGAGAGCCGCTCCAGCGGGCTGGGCCTTTACATCTGCCGGGAATTGTGCGAGCGTTACGGCGCCCTGATTG
GCTACCAGCGGGTGGCACGCCACGCCACCGAGGGCAACGAGTTTTTTGTCATCTTCAAGCCGGCGCCACAGCTTCTCGGC
ACCCCCCGGGGTTCTTTTGACACAATGCCTGCCTGA

Upstream 100 bases:

>100_bases
CGAGGTTTGCCATGTGCACCTGCCCCGGTCCGAGGCCCTGATCGGGTCCAGGGGCGGCATCTACTGCAGTGATGCGCACC
GCCGTGAGGCCGGAGGCTAG

Downstream 100 bases:

>100_bases
GACCCTACGCATACCCTAAGCATGCAAATACCCCCCACCTCACCCGCCCATATTCTTGTCGTCGATGACGAGCCGGACCT
GCGCACCCTGTACGAGCTGA

Product: periplasmic sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 571; Mature: 570

Protein sequence:

>571_residues
MTTPSQWFETAEKASGAWEFEPEFSSFGRLWRVFMTARVATAGVLVVLQAVIHALGNSSNRWPVAVCIAYLCATLAVRLW
AQPRPPGRTFDAHWLATIGVDVITFSLLEFLQSGGINYTPLFALPVLLSSILGPILLAFGTAASVTLLLLVDAWWTSLQT
PGDFNARFLQAGLSGSGFFLVALLANQLALRLAREEQRAKASQSAARMQTQVNELVIEALTDGVLVVDVNGVVRSANPAS
RRLLFMRKAIRNAPFMLATESAWQPLADLMHQTFATQSPQEADIRLEFPARSARRLHVRTRLAASQNGTHESLCVMFLED
LREMEARVRTEKMAAMGRMSAAVAHEIRNPLAAISQANALLEEDLQDAGHRQLTALVSQNAQRLAKIVDEVLNISRAQEQ
APAPQTTMLMLDDTVAQIATDWASQTGAAQRLGVTLNAAQTSVWFEAEHLRRLMVNLLDNALRYASASAHSMEVATRVMT
GGEVRLAVWSDGQPLEATVETHLFEPFFSSESRSSGLGLYICRELCERYGALIGYQRVARHATEGNEFFVIFKPAPQLLG
TPRGSFDTMPA

Sequences:

>Translated_571_residues
MTTPSQWFETAEKASGAWEFEPEFSSFGRLWRVFMTARVATAGVLVVLQAVIHALGNSSNRWPVAVCIAYLCATLAVRLW
AQPRPPGRTFDAHWLATIGVDVITFSLLEFLQSGGINYTPLFALPVLLSSILGPILLAFGTAASVTLLLLVDAWWTSLQT
PGDFNARFLQAGLSGSGFFLVALLANQLALRLAREEQRAKASQSAARMQTQVNELVIEALTDGVLVVDVNGVVRSANPAS
RRLLFMRKAIRNAPFMLATESAWQPLADLMHQTFATQSPQEADIRLEFPARSARRLHVRTRLAASQNGTHESLCVMFLED
LREMEARVRTEKMAAMGRMSAAVAHEIRNPLAAISQANALLEEDLQDAGHRQLTALVSQNAQRLAKIVDEVLNISRAQEQ
APAPQTTMLMLDDTVAQIATDWASQTGAAQRLGVTLNAAQTSVWFEAEHLRRLMVNLLDNALRYASASAHSMEVATRVMT
GGEVRLAVWSDGQPLEATVETHLFEPFFSSESRSSGLGLYICRELCERYGALIGYQRVARHATEGNEFFVIFKPAPQLLG
TPRGSFDTMPA
>Mature_570_residues
TTPSQWFETAEKASGAWEFEPEFSSFGRLWRVFMTARVATAGVLVVLQAVIHALGNSSNRWPVAVCIAYLCATLAVRLWA
QPRPPGRTFDAHWLATIGVDVITFSLLEFLQSGGINYTPLFALPVLLSSILGPILLAFGTAASVTLLLLVDAWWTSLQTP
GDFNARFLQAGLSGSGFFLVALLANQLALRLAREEQRAKASQSAARMQTQVNELVIEALTDGVLVVDVNGVVRSANPASR
RLLFMRKAIRNAPFMLATESAWQPLADLMHQTFATQSPQEADIRLEFPARSARRLHVRTRLAASQNGTHESLCVMFLEDL
REMEARVRTEKMAAMGRMSAAVAHEIRNPLAAISQANALLEEDLQDAGHRQLTALVSQNAQRLAKIVDEVLNISRAQEQA
PAPQTTMLMLDDTVAQIATDWASQTGAAQRLGVTLNAAQTSVWFEAEHLRRLMVNLLDNALRYASASAHSMEVATRVMTG
GEVRLAVWSDGQPLEATVETHLFEPFFSSESRSSGLGLYICRELCERYGALIGYQRVARHATEGNEFFVIFKPAPQLLGT
PRGSFDTMPA

Specific function: Member of the two-component regulatory system pilS/pilR involved in the regulation of the expression of type 4 fimbriae. PilS may function as a membrane-associated protein kinase that phosphorylates pilR in response to environmental signals [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=382, Percent_Identity=24.0837696335079, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1790436, Length=221, Percent_Identity=26.2443438914027, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1786912, Length=246, Percent_Identity=26.4227642276423, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1790300, Length=334, Percent_Identity=22.7544910179641, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 62692; Mature: 62560

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPSQWFETAEKASGAWEFEPEFSSFGRLWRVFMTARVATAGVLVVLQAVIHALGNSSN
CCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RWPVAVCIAYLCATLAVRLWAQPRPPGRTFDAHWLATIGVDVITFSLLEFLQSGGINYTP
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCH
LFALPVLLSSILGPILLAFGTAASVTLLLLVDAWWTSLQTPGDFNARFLQAGLSGSGFFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCHHHH
VALLANQLALRLAREEQRAKASQSAARMQTQVNELVIEALTDGVLVVDVNGVVRSANPAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEECCCHHH
RRLLFMRKAIRNAPFMLATESAWQPLADLMHQTFATQSPQEADIRLEFPARSARRLHVRT
HHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHH
RLAASQNGTHESLCVMFLEDLREMEARVRTEKMAAMGRMSAAVAHEIRNPLAAISQANAL
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEEDLQDAGHRQLTALVSQNAQRLAKIVDEVLNISRAQEQAPAPQTTMLMLDDTVAQIAT
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHH
DWASQTGAAQRLGVTLNAAQTSVWFEAEHLRRLMVNLLDNALRYASASAHSMEVATRVMT
HHHHHCCCHHHHCEEEECCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHC
GGEVRLAVWSDGQPLEATVETHLFEPFFSSESRSSGLGLYICRELCERYGALIGYQRVAR
CCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
HATEGNEFFVIFKPAPQLLGTPRGSFDTMPA
HCCCCCEEEEEECCCHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TTPSQWFETAEKASGAWEFEPEFSSFGRLWRVFMTARVATAGVLVVLQAVIHALGNSSN
CCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RWPVAVCIAYLCATLAVRLWAQPRPPGRTFDAHWLATIGVDVITFSLLEFLQSGGINYTP
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCH
LFALPVLLSSILGPILLAFGTAASVTLLLLVDAWWTSLQTPGDFNARFLQAGLSGSGFFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCHHHH
VALLANQLALRLAREEQRAKASQSAARMQTQVNELVIEALTDGVLVVDVNGVVRSANPAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEECCCHHH
RRLLFMRKAIRNAPFMLATESAWQPLADLMHQTFATQSPQEADIRLEFPARSARRLHVRT
HHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHH
RLAASQNGTHESLCVMFLEDLREMEARVRTEKMAAMGRMSAAVAHEIRNPLAAISQANAL
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEEDLQDAGHRQLTALVSQNAQRLAKIVDEVLNISRAQEQAPAPQTTMLMLDDTVAQIAT
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHH
DWASQTGAAQRLGVTLNAAQTSVWFEAEHLRRLMVNLLDNALRYASASAHSMEVATRVMT
HHHHHCCCHHHHCEEEECCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHC
GGEVRLAVWSDGQPLEATVETHLFEPFFSSESRSSGLGLYICRELCERYGALIGYQRVAR
CCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
HATEGNEFFVIFKPAPQLLGTPRGSFDTMPA
HCCCCCEEEEEECCCHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8097014; 10984043 [H]