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The map label for this gene is pilR [H]

Identifier: 91787008

GI number: 91787008

Start: 1144082

End: 1145524

Strand: Reverse

Name: pilR [H]

Synonym: Bpro_1110

Alternate gene names: 91787008

Gene position: 1145524-1144082 (Counterclockwise)

Preceding gene: 91787009

Following gene: 91787007

Centisome position: 22.03

GC content: 63.89

Gene sequence:

>1443_bases
ATGCAAATACCCCCCACCTCACCCGCCCATATTCTTGTCGTCGATGACGAGCCGGACCTGCGCACCCTGTACGAGCTGAC
CCTGCTTCGCGAAGGTTATCGGGTGGAAGCGGCCGGCGATTTGTTGCAGGCGCGCGAGCAGCTTGAGAGCAAGCGCTTCG
ATGCAGTCATTACCGACATGCGCCTGCCCGACGGGTTGGGACTGGAGCTGCTGCGTGAGCTGGTGGCCCAGCAACGCGCG
GAACGCTGCGTGATCATCACGGCGCACGGCTCTGCCGAAAATGCGGTGGAAGCCCTCAAGGCTGGCGCGTTCGACTACCT
GACCAAACCCGTTGACCTGAAACAGTTCCGCAGCGTCGTGGCGTCGGCCATCCTGGGCACGCAGAACCTGTCGCGACCCG
CCGCGGGCGATAGCGGTCTTGGCGAGCTGGGCCCGGGACCGACCGCGGCCACCTCAACCAAGTCGGAAAAATCTGACAAG
TCGGCGTCAGCCATCCTGCAAAAACTGGTGGGCGAATCGGCGGTGATGCGGGCCGTCAAGTCGCGCATCGTCAAGGTAGC
TGGCAGCATGGCACCGGTGCTGATACAGGGAGAATCCGGCACGGGCAAGGAACTGGTCGCACGCGCGGTGCACGCCTGCA
GCCACCGTGCGGCCGGTCCCTTTGTGGCCGTCAACTGCGGCGCCATTCCTGAAAACCTGCTTGAAGCCGAGTTTTTTGGC
GCCCGCAAAGGTGCCTACACCGGATCGACGCAGGACCGGCAAGGCTACTTTCAGGCCGCCAAGGGGGGAACCCTGTTTCT
CGATGAGATCGGTGACCTGCCGCTGGCCATGCAATCAAAGCTGCTGCGGGCGATCCAGGAACGCTCGGTGCGGCCGCTGG
GCTCCCCCCAGGAAGACACGGTAGATGTCCGCATCGTCAGCGCCACACACAAAGAGCTGGGCGCGCAGGTTCAGGCAGGC
GCCTTCCGGCAGGATCTGTATTACCGGCTCAACGTGATCGAAATCGGTGTGCCGCCGCTGCGCGAGCGGCGCGAAGACCT
GCCGGCACTTTGCCAGGCCCTGCTGTCAAGAATCTGCAAGGAGTCGGGGCTCGCTGTGCCTGAGCTCACCGGCGCGGTGA
TGGAGCAGCTGCGCTCGCATCCCTTTGACGGCAATGTCAGGGAGCTGGAAAACATCCTGCACCGCGCGGTGGCGCTCGGC
GAAGGCAATGACCTGCATTTCGAGGCCCCCCCCCAGGATTTCGTGGCGGTTCCGGAAAATCTGCAGGCGTTTCTCGACCA
GCAGGAGCGAAGCATCCTCTCCAAGGTGCTGCAGGAGACCGGGTTTAACCGCACCGCTGCCGCCTCCAAACTGGGGCTGA
GCCTGCGCCAGATCCGCTACCGCATTGCCCGTCTGAACATCGAGACACCCGGCGGCGATGACGCATCCGATGAGTCAGCC
TGA

Upstream 100 bases:

>100_bases
CGAGTTTTTTGTCATCTTCAAGCCGGCGCCACAGCTTCTCGGCACCCCCCGGGGTTCTTTTGACACAATGCCTGCCTGAG
ACCCTACGCATACCCTAAGC

Downstream 100 bases:

>100_bases
GCCGGACGGCACGCCGGCTGCCAGCCCGGCCTTGTGGCGGGACGGCTGGTACCGCTTTGCCCGCACGCTGGCGTCACCCA
ATTACGGCCCGCGACCCGGC

Product: Fis family two component sigma-54 specific transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MQIPPTSPAHILVVDDEPDLRTLYELTLLREGYRVEAAGDLLQAREQLESKRFDAVITDMRLPDGLGLELLRELVAQQRA
ERCVIITAHGSAENAVEALKAGAFDYLTKPVDLKQFRSVVASAILGTQNLSRPAAGDSGLGELGPGPTAATSTKSEKSDK
SASAILQKLVGESAVMRAVKSRIVKVAGSMAPVLIQGESGTGKELVARAVHACSHRAAGPFVAVNCGAIPENLLEAEFFG
ARKGAYTGSTQDRQGYFQAAKGGTLFLDEIGDLPLAMQSKLLRAIQERSVRPLGSPQEDTVDVRIVSATHKELGAQVQAG
AFRQDLYYRLNVIEIGVPPLRERREDLPALCQALLSRICKESGLAVPELTGAVMEQLRSHPFDGNVRELENILHRAVALG
EGNDLHFEAPPQDFVAVPENLQAFLDQQERSILSKVLQETGFNRTAAASKLGLSLRQIRYRIARLNIETPGGDDASDESA

Sequences:

>Translated_480_residues
MQIPPTSPAHILVVDDEPDLRTLYELTLLREGYRVEAAGDLLQAREQLESKRFDAVITDMRLPDGLGLELLRELVAQQRA
ERCVIITAHGSAENAVEALKAGAFDYLTKPVDLKQFRSVVASAILGTQNLSRPAAGDSGLGELGPGPTAATSTKSEKSDK
SASAILQKLVGESAVMRAVKSRIVKVAGSMAPVLIQGESGTGKELVARAVHACSHRAAGPFVAVNCGAIPENLLEAEFFG
ARKGAYTGSTQDRQGYFQAAKGGTLFLDEIGDLPLAMQSKLLRAIQERSVRPLGSPQEDTVDVRIVSATHKELGAQVQAG
AFRQDLYYRLNVIEIGVPPLRERREDLPALCQALLSRICKESGLAVPELTGAVMEQLRSHPFDGNVRELENILHRAVALG
EGNDLHFEAPPQDFVAVPENLQAFLDQQERSILSKVLQETGFNRTAAASKLGLSLRQIRYRIARLNIETPGGDDASDESA
>Mature_480_residues
MQIPPTSPAHILVVDDEPDLRTLYELTLLREGYRVEAAGDLLQAREQLESKRFDAVITDMRLPDGLGLELLRELVAQQRA
ERCVIITAHGSAENAVEALKAGAFDYLTKPVDLKQFRSVVASAILGTQNLSRPAAGDSGLGELGPGPTAATSTKSEKSDK
SASAILQKLVGESAVMRAVKSRIVKVAGSMAPVLIQGESGTGKELVARAVHACSHRAAGPFVAVNCGAIPENLLEAEFFG
ARKGAYTGSTQDRQGYFQAAKGGTLFLDEIGDLPLAMQSKLLRAIQERSVRPLGSPQEDTVDVRIVSATHKELGAQVQAG
AFRQDLYYRLNVIEIGVPPLRERREDLPALCQALLSRICKESGLAVPELTGAVMEQLRSHPFDGNVRELENILHRAVALG
EGNDLHFEAPPQDFVAVPENLQAFLDQQERSILSKVLQETGFNRTAAASKLGLSLRQIRYRIARLNIETPGGDDASDESA

Specific function: Member of the two-component regulatory system pilS/pilR that regulates the expression of type 4 fimbriae. When activated by pilS it acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=462, Percent_Identity=41.991341991342, Blast_Score=327, Evalue=9e-91,
Organism=Escherichia coli, GI1788550, Length=476, Percent_Identity=36.9747899159664, Blast_Score=308, Evalue=3e-85,
Organism=Escherichia coli, GI1788905, Length=449, Percent_Identity=40.0890868596882, Blast_Score=291, Evalue=6e-80,
Organism=Escherichia coli, GI1790299, Length=488, Percent_Identity=34.6311475409836, Blast_Score=264, Evalue=9e-72,
Organism=Escherichia coli, GI1789233, Length=233, Percent_Identity=50.2145922746781, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI87082117, Length=323, Percent_Identity=39.938080495356, Blast_Score=229, Evalue=4e-61,
Organism=Escherichia coli, GI1789087, Length=311, Percent_Identity=40.5144694533762, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI87082152, Length=318, Percent_Identity=39.3081761006289, Blast_Score=209, Evalue=4e-55,
Organism=Escherichia coli, GI87081872, Length=311, Percent_Identity=38.2636655948553, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1786524, Length=316, Percent_Identity=39.5569620253165, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1787583, Length=319, Percent_Identity=36.9905956112853, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1789828, Length=170, Percent_Identity=40, Blast_Score=132, Evalue=7e-32,
Organism=Escherichia coli, GI87081858, Length=219, Percent_Identity=35.1598173515982, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1786838, Length=108, Percent_Identity=37.037037037037, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 51795; Mature: 51795

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIPPTSPAHILVVDDEPDLRTLYELTLLREGYRVEAAGDLLQAREQLESKRFDAVITDM
CCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHHHHC
RLPDGLGLELLRELVAQQRAERCVIITAHGSAENAVEALKAGAFDYLTKPVDLKQFRSVV
CCCCCCCHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHCCHHHHCCCCCHHHHHHHH
ASAILGTQNLSRPAAGDSGLGELGPGPTAATSTKSEKSDKSASAILQKLVGESAVMRAVK
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHH
SRIVKVAGSMAPVLIQGESGTGKELVARAVHACSHRAAGPFVAVNCGAIPENLLEAEFFG
HHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHC
ARKGAYTGSTQDRQGYFQAAKGGTLFLDEIGDLPLAMQSKLLRAIQERSVRPLGSPQEDT
CCCCCCCCCCCCCHHHHHHCCCCEEEHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
VDVRIVSATHKELGAQVQAGAFRQDLYYRLNVIEIGVPPLRERREDLPALCQALLSRICK
EEEEEEEHHHHHHCCHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
ESGLAVPELTGAVMEQLRSHPFDGNVRELENILHRAVALGEGNDLHFEAPPQDFVAVPEN
HCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCEECCCCHHHHCCCHH
LQAFLDQQERSILSKVLQETGFNRTAAASKLGLSLRQIRYRIARLNIETPGGDDASDESA
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCC
>Mature Secondary Structure
MQIPPTSPAHILVVDDEPDLRTLYELTLLREGYRVEAAGDLLQAREQLESKRFDAVITDM
CCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHHHHC
RLPDGLGLELLRELVAQQRAERCVIITAHGSAENAVEALKAGAFDYLTKPVDLKQFRSVV
CCCCCCCHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHCCHHHHCCCCCHHHHHHHH
ASAILGTQNLSRPAAGDSGLGELGPGPTAATSTKSEKSDKSASAILQKLVGESAVMRAVK
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHH
SRIVKVAGSMAPVLIQGESGTGKELVARAVHACSHRAAGPFVAVNCGAIPENLLEAEFFG
HHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHC
ARKGAYTGSTQDRQGYFQAAKGGTLFLDEIGDLPLAMQSKLLRAIQERSVRPLGSPQEDT
CCCCCCCCCCCCCHHHHHHCCCCEEEHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
VDVRIVSATHKELGAQVQAGAFRQDLYYRLNVIEIGVPPLRERREDLPALCQALLSRICK
EEEEEEEHHHHHHCCHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
ESGLAVPELTGAVMEQLRSHPFDGNVRELENILHRAVALGEGNDLHFEAPPQDFVAVPEN
HCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCEECCCCHHHHCCCHH
LQAFLDQQERSILSKVLQETGFNRTAAASKLGLSLRQIRYRIARLNIETPGGDDASDESA
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1317379; 8097014; 7911557; 10984043 [H]