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The map label for this gene is ampD [H]

Identifier: 91787007

GI number: 91787007

Start: 1143490

End: 1144095

Strand: Reverse

Name: ampD [H]

Synonym: Bpro_1109

Alternate gene names: 91787007

Gene position: 1144095-1143490 (Counterclockwise)

Preceding gene: 91787008

Following gene: 91787005

Centisome position: 22.0

GC content: 63.86

Gene sequence:

>606_bases
ATGAGTCAGCCTGAGCCGGACGGCACGCCGGCTGCCAGCCCGGCCTTGTGGCGGGACGGCTGGTACCGCTTTGCCCGCAC
GCTGGCGTCACCCAATTACGGCCCGCGACCCGGCGGCGCACACATTGACCTGATCGTCATTCATTCCATCAGCCTGCCAC
CCGGGGAATTCGGCAACGGCAACGTGCAGCGCCTCTTCACCAACCAGCTCGACTGGGAAGCGCACCCTTACTTCCAGGGC
ATTCGCGGCCTGCAGGTTTCGTCTCACTTTTTCATCACCCGCGCAGGCGCGCTATGGCAATTTGTGGCTTGCCAAGACCG
TGCGTGGCATGCCGGCGAGTCCCGCTACCGGGGACGCAGCAACTGCAACGACGACTCCATCGGCATTGAACTGGAAGGAC
TGGAGGGCAGCACCTTTGAAGCACGCCAGTACGAGACCCTGGCGGGATTGTGCGCGGCGCTCCTTCAGGCCTATCCCATC
GCCCACATTGCGGGGCACGAACACATTGCGGCAGGGCGCAAGCAGGACCCCGGCCCGGGTTTTGACTGGCCCTTGCTGCA
ACGCTCACTGGCCGCACCCGATAGGTATTTTCCCGCAATCGACTGA

Upstream 100 bases:

>100_bases
CGCACCGCTGCCGCCTCCAAACTGGGGCTGAGCCTGCGCCAGATCCGCTACCGCATTGCCCGTCTGAACATCGAGACACC
CGGCGGCGATGACGCATCCG

Downstream 100 bases:

>100_bases
GCGGATTTGGGGGCGGCAGCCCCACGATGAAAATGCATCGGGTGGCTGAAAAATTCGCGGTATTTTTACCCATCGAGGAT
TCGCCGGCCTTGACAGAAGG

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 201; Mature: 200

Protein sequence:

>201_residues
MSQPEPDGTPAASPALWRDGWYRFARTLASPNYGPRPGGAHIDLIVIHSISLPPGEFGNGNVQRLFTNQLDWEAHPYFQG
IRGLQVSSHFFITRAGALWQFVACQDRAWHAGESRYRGRSNCNDDSIGIELEGLEGSTFEARQYETLAGLCAALLQAYPI
AHIAGHEHIAAGRKQDPGPGFDWPLLQRSLAAPDRYFPAID

Sequences:

>Translated_201_residues
MSQPEPDGTPAASPALWRDGWYRFARTLASPNYGPRPGGAHIDLIVIHSISLPPGEFGNGNVQRLFTNQLDWEAHPYFQG
IRGLQVSSHFFITRAGALWQFVACQDRAWHAGESRYRGRSNCNDDSIGIELEGLEGSTFEARQYETLAGLCAALLQAYPI
AHIAGHEHIAAGRKQDPGPGFDWPLLQRSLAAPDRYFPAID
>Mature_200_residues
SQPEPDGTPAASPALWRDGWYRFARTLASPNYGPRPGGAHIDLIVIHSISLPPGEFGNGNVQRLFTNQLDWEAHPYFQGI
RGLQVSSHFFITRAGALWQFVACQDRAWHAGESRYRGRSNCNDDSIGIELEGLEGSTFEARQYETLAGLCAALLQAYPIA
HIAGHEHIAAGRKQDPGPGFDWPLLQRSLAAPDRYFPAID

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786300, Length=170, Percent_Identity=48.2352941176471, Blast_Score=158, Evalue=3e-40,
Organism=Escherichia coli, GI1787092, Length=120, Percent_Identity=39.1666666666667, Blast_Score=69, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 22197; Mature: 22066

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQPEPDGTPAASPALWRDGWYRFARTLASPNYGPRPGGAHIDLIVIHSISLPPGEFGNG
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCC
NVQRLFTNQLDWEAHPYFQGIRGLQVSSHFFITRAGALWQFVACQDRAWHAGESRYRGRS
CEEEEECCCCCCCCCHHHHHHCCCEECCEEEEEECCHHHHHHHHCCCCCCCCCHHCCCCC
NCNDDSIGIELEGLEGSTFEARQYETLAGLCAALLQAYPIAHIAGHEHIAAGRKQDPGPG
CCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHCCCCCCCCCC
FDWPLLQRSLAAPDRYFPAID
CCHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SQPEPDGTPAASPALWRDGWYRFARTLASPNYGPRPGGAHIDLIVIHSISLPPGEFGNG
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCC
NVQRLFTNQLDWEAHPYFQGIRGLQVSSHFFITRAGALWQFVACQDRAWHAGESRYRGRS
CEEEEECCCCCCCCCHHHHHHCCCEECCEEEEEECCHHHHHHHHCCCCCCCCCHHCCCCC
NCNDDSIGIELEGLEGSTFEARQYETLAGLCAALLQAYPIAHIAGHEHIAAGRKQDPGPG
CCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHCCCCCCCCCC
FDWPLLQRSLAAPDRYFPAID
CCHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383940 [H]