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The map label for this gene is potB [H]

Identifier: 91786909

GI number: 91786909

Start: 1024135

End: 1024971

Strand: Reverse

Name: potB [H]

Synonym: Bpro_1009

Alternate gene names: 91786909

Gene position: 1024971-1024135 (Counterclockwise)

Preceding gene: 91786910

Following gene: 91786908

Centisome position: 19.71

GC content: 57.59

Gene sequence:

>837_bases
ATGCCGCTGACGACGGTCTTTGTGCTCTTTTTCCTCATTCCGCTGGCGCTGATCCTGATGGTCAGCTTCTGGGATTTCAA
CGAATACGAGCTGCTGCCCGGCTTCACCCTGAAGAATTATTTTTCCATCTTCGAAGGCTGCGGCAACACCTCCGACCTGT
GCGTCACCTTCAAGACCTACCTGTCGACCTTCAAGTTCAGCTTTCTGGTCTGGCTGATCACGCTGGTGACCGGCTTTGCC
GTGTCGTATTTCCTGGCTTTTCACGTGCGCTCGCAAGGCGTGCAGACGCTGCTGTTCGTGCTGTGCACCATTCCTTTCTG
GACCTCCAACGTGATCCGCATGATCTCGTGGGTGCCGCTGCTGGGCCGCAACGGCCTGGTCAACCAGAGCCTGGCCGGCC
TGGGCGTGGTGAACCAGCCCATTGAGTGGCTGCTGTTTTCTGACTTTTCGGTAGTGCTGGCGTTTGTGCACCTCTACACC
ATGTTCATGATCGTTCCGATTTTCAATTCGATGATGCGCATAGACCGCAACCTGATCGAAGCAGCCAGCGACAGCGGTGC
CTCGGCCTGGCAAACACTGTGGAACGTCATCGTGCCGCTGTCGCGCACGGGCATCATCATCGGCTCGATTTTTGTCATCA
CCATCGTGATGGGCGACTTCGTCACCATCGGTGTCATGGGCGGCCAGCAGATCGCCTCCATCGGCAAGATCATCCAGGTG
CAGACGTCCTACCTGCAGTTCCCGCTGGCCGCGGCCAATGCGGTCATCCTGCTGGCGTTGGTGCTCATGATCATCTGGGC
ACTGACCCGCATGGTCGACATCCGCAAGGAACTCTGA

Upstream 100 bases:

>100_bases
GGGAACAAGACCCCAAAACATGAGCACACCTACCGCCTCCTTGCCTTCACCCGCGCCCGCGCCCGCGCCGGGCCGCAGCC
TTGCCGCCTGGTGGCAGGCC

Downstream 100 bases:

>100_bases
ATGAACAGCGACAAACGCACCGCCGGCTTCTGGTTCCTGGCCGCTTTTTTCGCCCTGTTCGTGCTGTTCCTCTACGGGCC
CATGTTCGTCATCGTGGTGC

Product: binding-protein-dependent transport systems inner membrane component

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MPLTTVFVLFFLIPLALILMVSFWDFNEYELLPGFTLKNYFSIFEGCGNTSDLCVTFKTYLSTFKFSFLVWLITLVTGFA
VSYFLAFHVRSQGVQTLLFVLCTIPFWTSNVIRMISWVPLLGRNGLVNQSLAGLGVVNQPIEWLLFSDFSVVLAFVHLYT
MFMIVPIFNSMMRIDRNLIEAASDSGASAWQTLWNVIVPLSRTGIIIGSIFVITIVMGDFVTIGVMGGQQIASIGKIIQV
QTSYLQFPLAAANAVILLALVLMIIWALTRMVDIRKEL

Sequences:

>Translated_278_residues
MPLTTVFVLFFLIPLALILMVSFWDFNEYELLPGFTLKNYFSIFEGCGNTSDLCVTFKTYLSTFKFSFLVWLITLVTGFA
VSYFLAFHVRSQGVQTLLFVLCTIPFWTSNVIRMISWVPLLGRNGLVNQSLAGLGVVNQPIEWLLFSDFSVVLAFVHLYT
MFMIVPIFNSMMRIDRNLIEAASDSGASAWQTLWNVIVPLSRTGIIIGSIFVITIVMGDFVTIGVMGGQQIASIGKIIQV
QTSYLQFPLAAANAVILLALVLMIIWALTRMVDIRKEL
>Mature_277_residues
PLTTVFVLFFLIPLALILMVSFWDFNEYELLPGFTLKNYFSIFEGCGNTSDLCVTFKTYLSTFKFSFLVWLITLVTGFAV
SYFLAFHVRSQGVQTLLFVLCTIPFWTSNVIRMISWVPLLGRNGLVNQSLAGLGVVNQPIEWLLFSDFSVVLAFVHLYTM
FMIVPIFNSMMRIDRNLIEAASDSGASAWQTLWNVIVPLSRTGIIIGSIFVITIVMGDFVTIGVMGGQQIASIGKIIQVQ
TSYLQFPLAAANAVILLALVLMIIWALTRMVDIRKEL

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=276, Percent_Identity=31.5217391304348, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI1787080, Length=218, Percent_Identity=34.4036697247706, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1787713, Length=262, Percent_Identity=24.4274809160305, Blast_Score=81, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31188; Mature: 31057

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PLTTVFVLFFLIPLALILMVSFWDFNEYELLPGFTLKNYFSIFEGCGNTSDLCVTFKTY
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHH
LSTFKFSFLVWLITLVTGFAVSYFLAFHVRSQGVQTLLFVLCTIPFWTSNVIRMISWVPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LGRNGLVNQSLAGLGVVNQPIEWLLFSDFSVVLAFVHLYTMFMIVPIFNSMMRIDRNLIE
HCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AASDSGASAWQTLWNVIVPLSRTGIIIGSIFVITIVMGDFVTIGVMGGQQIASIGKIIQV
HHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHH
QTSYLQFPLAAANAVILLALVLMIIWALTRMVDIRKEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]