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The map label for this gene is hpcG [H]

Identifier: 91786873

GI number: 91786873

Start: 990357

End: 991139

Strand: Reverse

Name: hpcG [H]

Synonym: Bpro_0971

Alternate gene names: 91786873

Gene position: 991139-990357 (Counterclockwise)

Preceding gene: 91786874

Following gene: 91786872

Centisome position: 19.06

GC content: 58.11

Gene sequence:

>783_bases
ATGATCAATGCCAAACAACGTCAGGACGCCGCAAATGCGTTGCTGCAAGCCGAAAAAGACCGTCAGCCCATCCAGCAGGT
CAGCAAGACCTGGCCGGAGATGGAGATTGAAGATTCATATGCCATTCAGCAACTTTGGACAGCGGCCCGTGTGAAAGCGG
GCGCGCGCGTTGTCGGTCACAAAATTGGACTTACGTCCAAGGCCATGCAGAGGGCCTCCAAGATGACGGAGCCCGACTAC
GGTGTGTTGCTCGATGACATGCTTTATCCGGACGGCGCAAAGATTCCCGCTTCACGATTCCATATGCCGAGGCTGGAGGT
CGAGTTGGCCTTCGTTTTGGGCAAACCGCTCGGCGGCAAGAACCTCACCGTTTATGACGTGCTCGATGCAACGGCATATG
TCACGCCGGCACTGGAAATCATTGATTACCGCACCGAAGTTCCACGCGAAATTTGCGACACCATCGCAGATAACGCCGCG
GCAGCTGCCATGGTGACGGGTGGCCGGGTCGTCCGGCCCATGGATGTGGACCTTCGCTGGATAGGCGCCACGCTGTCCAA
GAATGGTGTCATTGAGGAGTCCGGCGTTTCGGCGGCAGTCATGGGTCACCCCGCAATGGGGATTGTGTGGCTTGCCAACA
AACTCGCAGTACACGGCATCACGCTGGAGGCGGGGCACATCCTTTTGGCCGGATCATTTACCCGGCCAACCGCGGTTGAG
GCTGGTGACACCATCCATGCAGACTTCGGCCCCCTGGGCTCCATCGGCCTCTCATTTTCGTGA

Upstream 100 bases:

>100_bases
CGCGTGTGGTTTCAATTCTCCTTTGCAACGGAAGTTTGAAATTCTCTTTTTTTGCTCCGGGGCGTCGTGACGCCTCGTCG
CACACATATAAAGCCAGACA

Downstream 100 bases:

>100_bases
GCGGTCGCGCGCCATGCAACTGCCAATAAATCACTTCAAACAAGCCCTGCTTCAGGGTACACAGCAGCTCGGGCTTTGGT
GCACATTGTCCAGTCCCTAC

Product: 2-oxo-hepta-3-ene-1,7-dioic acid hydratase

Products: NA

Alternate protein names: OHED hydratase [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MINAKQRQDAANALLQAEKDRQPIQQVSKTWPEMEIEDSYAIQQLWTAARVKAGARVVGHKIGLTSKAMQRASKMTEPDY
GVLLDDMLYPDGAKIPASRFHMPRLEVELAFVLGKPLGGKNLTVYDVLDATAYVTPALEIIDYRTEVPREICDTIADNAA
AAAMVTGGRVVRPMDVDLRWIGATLSKNGVIEESGVSAAVMGHPAMGIVWLANKLAVHGITLEAGHILLAGSFTRPTAVE
AGDTIHADFGPLGSIGLSFS

Sequences:

>Translated_260_residues
MINAKQRQDAANALLQAEKDRQPIQQVSKTWPEMEIEDSYAIQQLWTAARVKAGARVVGHKIGLTSKAMQRASKMTEPDY
GVLLDDMLYPDGAKIPASRFHMPRLEVELAFVLGKPLGGKNLTVYDVLDATAYVTPALEIIDYRTEVPREICDTIADNAA
AAAMVTGGRVVRPMDVDLRWIGATLSKNGVIEESGVSAAVMGHPAMGIVWLANKLAVHGITLEAGHILLAGSFTRPTAVE
AGDTIHADFGPLGSIGLSFS
>Mature_260_residues
MINAKQRQDAANALLQAEKDRQPIQQVSKTWPEMEIEDSYAIQQLWTAARVKAGARVVGHKIGLTSKAMQRASKMTEPDY
GVLLDDMLYPDGAKIPASRFHMPRLEVELAFVLGKPLGGKNLTVYDVLDATAYVTPALEIIDYRTEVPREICDTIADNAA
AAAMVTGGRVVRPMDVDLRWIGATLSKNGVIEESGVSAAVMGHPAMGIVWLANKLAVHGITLEAGHILLAGSFTRPTAVE
AGDTIHADFGPLGSIGLSFS

Specific function: Transforms 2-oxo-hept-3-ene-1,7-dioate (OHED) into 2,4- dihydoxy-hept-2-ene-1,7-dioic acid (HHED) [H]

COG id: COG3971

COG function: function code Q; 2-keto-4-pentenoate hydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hydratase/decarboxylase family [H]

Homologues:

Organism=Escherichia coli, GI87081722, Length=225, Percent_Identity=37.7777777777778, Blast_Score=151, Evalue=5e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234
- InterPro:   IPR012690 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: 4.2.1.-

Molecular weight: Translated: 27947; Mature: 27947

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINAKQRQDAANALLQAEKDRQPIQQVSKTWPEMEIEDSYAIQQLWTAARVKAGARVVGH
CCCCHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHH
KIGLTSKAMQRASKMTEPDYGVLLDDMLYPDGAKIPASRFHMPRLEVELAFVLGKPLGGK
HCCCCHHHHHHHHHCCCCCCCEEEHHHCCCCCCCCCHHHCCCCCHHEEEEEEECCCCCCC
NLTVYDVLDATAYVTPALEIIDYRTEVPREICDTIADNAAAAAMVTGGRVVRPMDVDLRW
CEEEEEECCHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHEEEECCCEEEEECCCCEEE
IGATLSKNGVIEESGVSAAVMGHPAMGIVWLANKLAVHGITLEAGHILLAGSFTRPTAVE
EEEECCCCCCEECCCCEEEEECCCCHHHEEHHHHHEEEEEEEECCCEEEEECCCCCCCCC
AGDTIHADFGPLGSIGLSFS
CCCEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MINAKQRQDAANALLQAEKDRQPIQQVSKTWPEMEIEDSYAIQQLWTAARVKAGARVVGH
CCCCHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHH
KIGLTSKAMQRASKMTEPDYGVLLDDMLYPDGAKIPASRFHMPRLEVELAFVLGKPLGGK
HCCCCHHHHHHHHHCCCCCCCEEEHHHCCCCCCCCCHHHCCCCCHHEEEEEEECCCCCCC
NLTVYDVLDATAYVTPALEIIDYRTEVPREICDTIADNAAAAAMVTGGRVVRPMDVDLRW
CEEEEEECCHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHEEEECCCEEEEECCCCEEE
IGATLSKNGVIEESGVSAAVMGHPAMGIVWLANKLAVHGITLEAGHILLAGSFTRPTAVE
EEEECCCCCCEECCCCEEEEECCCCHHHEEHHHHHEEEEEEEECCCEEEEECCCCCCCCC
AGDTIHADFGPLGSIGLSFS
CCCEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7737515 [H]