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The map label for this gene is bclA [H]

Identifier: 91786495

GI number: 91786495

Start: 592430

End: 594073

Strand: Reverse

Name: bclA [H]

Synonym: Bpro_0589

Alternate gene names: 91786495

Gene position: 594073-592430 (Counterclockwise)

Preceding gene: 91786496

Following gene: 91786494

Centisome position: 11.42

GC content: 66.42

Gene sequence:

>1644_bases
ATGAATACGCCCACACACGCACCAGCCTCGAAGGCGCCCTCTGCGCAGACCGACTGCTTCGTGCACGACCGCCTGCCAAC
GCCCGACCAGTGGCCGAAACTGGTCTACCCGCCCGAACTGCGCATTCCCGACCAGGCCAATCTGGTGGACGTGCTGTTCT
CCCGGGCCAGGGCGCAAGGACATGCCGACAAGCCCTTCCTGCGTTCGGACCGGATCACCTTGAGCTATGCCGACGCACTG
GAGCGCGTCAACCGCATCGCCCAGGTGCTGACGGAAGACTTTCACCTCGTGCCGGGCAACCGCGTGCTGCTGCGCGGCGG
CAACTCCATCGGCATGGCGCTGGCCTGGCTGGGTGTGGTGCAGGCCGGGCTGGTGGCGGTGGCGACCATGCCGCTGCTGC
GCGCCAAGGAGCTCGGCGAGATCATTGACAAGGCCCGGCCGTCGCTGGCACTGTGCGATGCCAGCCTGCTGCAGGAATTG
CAGGCCGCCAGGGATCAGGGCGGCCTGCTCGGCACCATCGTGCCGTTCAACCTGATGAACGAGCCCGGTTCGCTGGCGGT
CCTTTCCGCACAGAAAGACGGCCATTTCACGCCCTGCCCGACGGCCGCCGACGACATTGCGATGATGGCCTTCACGTCCG
GCACCACCGGCAAGCCCAAGGCCGCCGTGCACACGCACCGCGATGTGCTCGCCGCCTGCGAAGCCTGGCCGCGCCATGTG
CTCAAGGCCAGGCCTGACGACATCGTGATGGGTTCGCCGCCACTCGCCTTCACCTTCGGGCTGGGCGGCATGCTGATCTT
CCCGATGTGGGCTGGTGCCTCGGTGTACTACCCCAGCGTGGCCTACACGCCCGAGGTCATGGTCAAGCTGATGAACCAGG
TCGGCGCCACCCTGTGCTACACGGCGCCCACCTTTTACCGCCAGATGGCGCCGTTTGCCAAACAGCTCGGCGTGCCCAGC
CTGCGCATCTGCGTGAGTGCGGGCGAGGGCCTGCCGGACGCCACACGCCAGCTCTGGAAGGACGCCACCGGCATTGAAAT
GATCGACGGGATAGGCGCGACCGAGATGTTCCATATCTTCATCTCGTCCGCCGGTGCCGAGGTACGGCGCGGCGCCATCG
GCAAGGTGGTGCCCGGCTACACCGCCCGGGTGGTGGACGACCAGGGTGCGGAAGTGCCGCATGGCACCGTCGGCAAGCTG
GCGGTCATGGGGCCGACTGGCTGCAAATACCTGGACGATGCGCGCCAGGCGAACTATGTCAAGGGCGGCTGGAACTACCC
CGGCGATGCGTTTGTGCAGGACGATGACGGCTACTTCTTCTACCAAGCCCGCGCCGACGACATGATCATCACGTCGGGCT
ACAACGTGGGCGGCCCCGAGGTGGAAGACGCGCTGCTCAAGCACCCGGCCGTGGCCGAGTGCGGCGTGATCGGCCTGCCC
GACGAGGAACGCGGCATGATCGTCAAGGCCTTTGTGGTGCTGCGGCCCGGCCACGAGGCCGGCGAGGCCCTGGTCAAGAG
CCTGCAGGACCACGTCAAGGCCACGCTCGCGCCCTTCAAATACCCGCGCCAGATCGAATTCGTGGGCAGCCTGCCGCGGA
CCGAAACCGGCAAACTGCAACGTTTCAAATTGCGTCAATCATGA

Upstream 100 bases:

>100_bases
GCAAGGTGGAAAGCCTGTACCGTGACATCCGCTCGCTGCGGATTTACGAAGGCGCCACCGAGGTCCAGCAACTCATCATC
GGCAAGGCGGTGTTGCAATC

Downstream 100 bases:

>100_bases
AATTCACGACACAAAAGCAGGTCCGGTTTCACCACTGCGACCCGGCGGGGATTGTTTTTTACCCGCAGTTTTTCTACCTG
TTGCATGAGGTGCAGGAGGA

Product: benzoate-CoA ligase family

Products: NA

Alternate protein names: Benzoyl-CoA synthetase [H]

Number of amino acids: Translated: 547; Mature: 547

Protein sequence:

>547_residues
MNTPTHAPASKAPSAQTDCFVHDRLPTPDQWPKLVYPPELRIPDQANLVDVLFSRARAQGHADKPFLRSDRITLSYADAL
ERVNRIAQVLTEDFHLVPGNRVLLRGGNSIGMALAWLGVVQAGLVAVATMPLLRAKELGEIIDKARPSLALCDASLLQEL
QAARDQGGLLGTIVPFNLMNEPGSLAVLSAQKDGHFTPCPTAADDIAMMAFTSGTTGKPKAAVHTHRDVLAACEAWPRHV
LKARPDDIVMGSPPLAFTFGLGGMLIFPMWAGASVYYPSVAYTPEVMVKLMNQVGATLCYTAPTFYRQMAPFAKQLGVPS
LRICVSAGEGLPDATRQLWKDATGIEMIDGIGATEMFHIFISSAGAEVRRGAIGKVVPGYTARVVDDQGAEVPHGTVGKL
AVMGPTGCKYLDDARQANYVKGGWNYPGDAFVQDDDGYFFYQARADDMIITSGYNVGGPEVEDALLKHPAVAECGVIGLP
DEERGMIVKAFVVLRPGHEAGEALVKSLQDHVKATLAPFKYPRQIEFVGSLPRTETGKLQRFKLRQS

Sequences:

>Translated_547_residues
MNTPTHAPASKAPSAQTDCFVHDRLPTPDQWPKLVYPPELRIPDQANLVDVLFSRARAQGHADKPFLRSDRITLSYADAL
ERVNRIAQVLTEDFHLVPGNRVLLRGGNSIGMALAWLGVVQAGLVAVATMPLLRAKELGEIIDKARPSLALCDASLLQEL
QAARDQGGLLGTIVPFNLMNEPGSLAVLSAQKDGHFTPCPTAADDIAMMAFTSGTTGKPKAAVHTHRDVLAACEAWPRHV
LKARPDDIVMGSPPLAFTFGLGGMLIFPMWAGASVYYPSVAYTPEVMVKLMNQVGATLCYTAPTFYRQMAPFAKQLGVPS
LRICVSAGEGLPDATRQLWKDATGIEMIDGIGATEMFHIFISSAGAEVRRGAIGKVVPGYTARVVDDQGAEVPHGTVGKL
AVMGPTGCKYLDDARQANYVKGGWNYPGDAFVQDDDGYFFYQARADDMIITSGYNVGGPEVEDALLKHPAVAECGVIGLP
DEERGMIVKAFVVLRPGHEAGEALVKSLQDHVKATLAPFKYPRQIEFVGSLPRTETGKLQRFKLRQS
>Mature_547_residues
MNTPTHAPASKAPSAQTDCFVHDRLPTPDQWPKLVYPPELRIPDQANLVDVLFSRARAQGHADKPFLRSDRITLSYADAL
ERVNRIAQVLTEDFHLVPGNRVLLRGGNSIGMALAWLGVVQAGLVAVATMPLLRAKELGEIIDKARPSLALCDASLLQEL
QAARDQGGLLGTIVPFNLMNEPGSLAVLSAQKDGHFTPCPTAADDIAMMAFTSGTTGKPKAAVHTHRDVLAACEAWPRHV
LKARPDDIVMGSPPLAFTFGLGGMLIFPMWAGASVYYPSVAYTPEVMVKLMNQVGATLCYTAPTFYRQMAPFAKQLGVPS
LRICVSAGEGLPDATRQLWKDATGIEMIDGIGATEMFHIFISSAGAEVRRGAIGKVVPGYTARVVDDQGAEVPHGTVGKL
AVMGPTGCKYLDDARQANYVKGGWNYPGDAFVQDDDGYFFYQARADDMIITSGYNVGGPEVEDALLKHPAVAECGVIGLP
DEERGMIVKAFVVLRPGHEAGEALVKSLQDHVKATLAPFKYPRQIEFVGSLPRTETGKLQRFKLRQS

Specific function: Catalyzes the ligation of benzoate and CoA to form benzoyl-CoA at the expense of ATP. The enzyme also ligates 2- aminobenzoate and CoA. The enzyme shows activity toward a number of benzoate derivates [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. Benzoate-CoA ligase subfamily [H]

Homologues:

Organism=Homo sapiens, GI42544132, Length=531, Percent_Identity=30.8851224105461, Blast_Score=233, Evalue=4e-61,
Organism=Homo sapiens, GI38505220, Length=531, Percent_Identity=31.638418079096, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI122937307, Length=496, Percent_Identity=32.0564516129032, Blast_Score=211, Evalue=2e-54,
Organism=Homo sapiens, GI115511026, Length=493, Percent_Identity=29.0060851926978, Blast_Score=202, Evalue=5e-52,
Organism=Homo sapiens, GI58082049, Length=502, Percent_Identity=29.6812749003984, Blast_Score=197, Evalue=3e-50,
Organism=Homo sapiens, GI157311624, Length=502, Percent_Identity=29.2828685258964, Blast_Score=195, Evalue=1e-49,
Organism=Homo sapiens, GI157311622, Length=502, Percent_Identity=29.2828685258964, Blast_Score=195, Evalue=1e-49,
Organism=Homo sapiens, GI187761345, Length=543, Percent_Identity=26.3351749539595, Blast_Score=143, Evalue=5e-34,
Organism=Homo sapiens, GI187761343, Length=543, Percent_Identity=26.3351749539595, Blast_Score=143, Evalue=5e-34,
Organism=Homo sapiens, GI156151445, Length=526, Percent_Identity=23.9543726235741, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI28416953, Length=384, Percent_Identity=28.3854166666667, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI13375727, Length=527, Percent_Identity=23.1499051233397, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI253970489, Length=365, Percent_Identity=27.9452054794521, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI8923896, Length=365, Percent_Identity=27.9452054794521, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI42544134, Length=361, Percent_Identity=24.6537396121884, Blast_Score=93, Evalue=6e-19,
Organism=Homo sapiens, GI195947378, Length=450, Percent_Identity=22.4444444444444, Blast_Score=86, Evalue=1e-16,
Organism=Escherichia coli, GI1788107, Length=547, Percent_Identity=27.7879341864717, Blast_Score=168, Evalue=8e-43,
Organism=Escherichia coli, GI145693145, Length=465, Percent_Identity=27.3118279569892, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1790505, Length=363, Percent_Identity=29.4765840220386, Blast_Score=139, Evalue=6e-34,
Organism=Escherichia coli, GI221142682, Length=493, Percent_Identity=25.1521298174442, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI1789201, Length=350, Percent_Identity=28.2857142857143, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1786810, Length=497, Percent_Identity=23.7424547283702, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1786529, Length=372, Percent_Identity=26.3440860215054, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1786801, Length=441, Percent_Identity=23.5827664399093, Blast_Score=70, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17558820, Length=513, Percent_Identity=25.3411306042885, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI32563687, Length=515, Percent_Identity=25.4368932038835, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI71994703, Length=534, Percent_Identity=25.0936329588015, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI71994694, Length=534, Percent_Identity=25.0936329588015, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71994690, Length=534, Percent_Identity=25.0936329588015, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17557194, Length=369, Percent_Identity=28.7262872628726, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17531443, Length=496, Percent_Identity=24.3951612903226, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17538037, Length=372, Percent_Identity=27.1505376344086, Blast_Score=114, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI17560308, Length=355, Percent_Identity=28.7323943661972, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71996755, Length=501, Percent_Identity=24.750499001996, Blast_Score=108, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71982997, Length=366, Percent_Identity=27.8688524590164, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71983001, Length=366, Percent_Identity=27.8688524590164, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17559526, Length=363, Percent_Identity=26.4462809917355, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71985884, Length=349, Percent_Identity=25.7879656160458, Blast_Score=83, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6323182, Length=541, Percent_Identity=25.3234750462107, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6319264, Length=528, Percent_Identity=24.4318181818182, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6319699, Length=346, Percent_Identity=27.1676300578035, Blast_Score=111, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24581924, Length=350, Percent_Identity=31.1428571428571, Blast_Score=147, Evalue=3e-35,
Organism=Drosophila melanogaster, GI18859661, Length=460, Percent_Identity=24.5652173913043, Blast_Score=116, Evalue=5e-26,
Organism=Drosophila melanogaster, GI62472339, Length=552, Percent_Identity=23.3695652173913, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24667955, Length=527, Percent_Identity=23.5294117647059, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24667959, Length=365, Percent_Identity=26.3013698630137, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI21355181, Length=486, Percent_Identity=23.0452674897119, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI19922652, Length=501, Percent_Identity=22.5548902195609, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI21355225, Length=378, Percent_Identity=23.5449735449735, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24648260, Length=510, Percent_Identity=22.9411764705882, Blast_Score=86, Evalue=9e-17,
Organism=Drosophila melanogaster, GI21356947, Length=514, Percent_Identity=22.568093385214, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI21356441, Length=415, Percent_Identity=22.8915662650602, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24648257, Length=505, Percent_Identity=20.990099009901, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24653035, Length=487, Percent_Identity=22.3819301848049, Blast_Score=67, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011957 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.25 [H]

Molecular weight: Translated: 59030; Mature: 59030

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTPTHAPASKAPSAQTDCFVHDRLPTPDQWPKLVYPPELRIPDQANLVDVLFSRARAQG
CCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
HADKPFLRSDRITLSYADALERVNRIAQVLTEDFHLVPGNRVLLRGGNSIGMALAWLGVV
CCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCEECCCCEEEEECCCCHHHHHHHHHHH
QAGLVAVATMPLLRAKELGEIIDKARPSLALCDASLLQELQAARDQGGLLGTIVPFNLMN
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCEECC
EPGSLAVLSAQKDGHFTPCPTAADDIAMMAFTSGTTGKPKAAVHTHRDVLAACEAWPRHV
CCCCEEEEEECCCCCCCCCCCCHHHEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHH
LKARPDDIVMGSPPLAFTFGLGGMLIFPMWAGASVYYPSVAYTPEVMVKLMNQVGATLCY
HHCCCCCEEECCCCCEEEECCCCEEEEHHHCCCEEECCCCCCCHHHHHHHHHHHCCEEEE
TAPTFYRQMAPFAKQLGVPSLRICVSAGEGLPDATRQLWKDATGIEMIDGIGATEMFHIF
CCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHCCCCCCHHHCCCCHHHHHHHH
ISSAGAEVRRGAIGKVVPGYTARVVDDQGAEVPHGTVGKLAVMGPTGCKYLDDARQANYV
HHCCCHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCHHHHHHHCCCC
KGGWNYPGDAFVQDDDGYFFYQARADDMIITSGYNVGGPEVEDALLKHPAVAECGVIGLP
CCCCCCCCCCEEECCCCEEEEEECCCCEEEECCCCCCCCHHHHHHHCCCCHHHCCEECCC
DEERGMIVKAFVVLRPGHEAGEALVKSLQDHVKATLAPFKYPRQIEFVGSLPRTETGKLQ
CCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCHH
RFKLRQS
HEECCCC
>Mature Secondary Structure
MNTPTHAPASKAPSAQTDCFVHDRLPTPDQWPKLVYPPELRIPDQANLVDVLFSRARAQG
CCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
HADKPFLRSDRITLSYADALERVNRIAQVLTEDFHLVPGNRVLLRGGNSIGMALAWLGVV
CCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCEECCCCEEEEECCCCHHHHHHHHHHH
QAGLVAVATMPLLRAKELGEIIDKARPSLALCDASLLQELQAARDQGGLLGTIVPFNLMN
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCEECC
EPGSLAVLSAQKDGHFTPCPTAADDIAMMAFTSGTTGKPKAAVHTHRDVLAACEAWPRHV
CCCCEEEEEECCCCCCCCCCCCHHHEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHH
LKARPDDIVMGSPPLAFTFGLGGMLIFPMWAGASVYYPSVAYTPEVMVKLMNQVGATLCY
HHCCCCCEEECCCCCEEEECCCCEEEEHHHCCCEEECCCCCCCHHHHHHHHHHHCCEEEE
TAPTFYRQMAPFAKQLGVPSLRICVSAGEGLPDATRQLWKDATGIEMIDGIGATEMFHIF
CCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHCCCCCCHHHCCCCHHHHHHHH
ISSAGAEVRRGAIGKVVPGYTARVVDDQGAEVPHGTVGKLAVMGPTGCKYLDDARQANYV
HHCCCHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCHHHHHHHCCCC
KGGWNYPGDAFVQDDDGYFFYQARADDMIITSGYNVGGPEVEDALLKHPAVAECGVIGLP
CCCCCCCCCCEEECCCCEEEEEECCCCEEEECCCCCCCCHHHHHHHCCCCHHHCCEECCC
DEERGMIVKAFVVLRPGHEAGEALVKSLQDHVKATLAPFKYPRQIEFVGSLPRTETGKLQ
CCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCHH
RFKLRQS
HEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12897012 [H]