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The map label for this gene is amiE [H]

Identifier: 91786477

GI number: 91786477

Start: 573009

End: 574046

Strand: Direct

Name: amiE [H]

Synonym: Bpro_0571

Alternate gene names: 91786477

Gene position: 573009-574046 (Clockwise)

Preceding gene: 91786476

Following gene: 91786492

Centisome position: 11.02

GC content: 59.06

Gene sequence:

>1038_bases
ATGATTCACGGCGATATTTCAAGCAGCAAGGACACTGTCGGCGTCGCGGTGGTCAACTACAAAATGCCGCGCCTGCACAG
CCAGGCCGACGTCATGGAGAATGCGCGCAACATTGCCCGCATGGTCGAGGGCATGAAGCAGGGGCTGCCCGGGCTGGACC
TGGTGATCTTTCCGGAGTACAGCACCCACGGCATCATGTACGACAGCAAAGAGATGCATGAGAACGCGGCCACAGTGCCT
GGCTTCGAAACCGATATCTTTGCAGCCGCCTGCCGAAAGGCCAAGGTTTGGGGCGTGTTCTCGCTCACCGGTGAACGGCA
TGAAGACCATCCGCGCAAGGCGCCGTACAACACCCTGATCCTGATGAACGACCGGGGCGAGATTGTCCAGAAATACCGCA
AGATCATGCCCTGGACTCCTGTCGAAGGCTGGTACCCGGGTGACCGCACCTACGTCAGTGAGGGACCCAAGGGCCTGAAG
GTCAGCCTGATCATTTGCGACGACGGCAACTACCCGGAGATCTGGCGTGATTGTGCAATGCGGGGGGCCGAACTGATTGT
GCGCTGCCAGGGCTATATGTACCCGGCCAAGGAGCAACAGATCCTGATCTCCAAGGCCATGGCGTTTGCCAACAACACCT
ATGTGGCCGTGGCCAATGCGGCGGGCTTTGATGGCGTGTACTCCTATTTTGGACACTCTGCGCTGATCGGCTTTGACGGC
AGAACGCTGGGCGAGTGTGGCGAGGAGGAAATGGGCATTCAGTATGCGGCGCTGTCCAAGAGCCTGATTCGCGACTTTCG
CCGCAATGGCCAGTCAGAGAACCATCTCTTCAAGCTGGTACACCGCGGCTATACGGGGGTGATCAATTCGGGTGATGGGG
ACAAGGGTGTGGCCGCCTGCCCCTACGACTTTTATGCCAACTGGCTGCGTGATCCCGAGGGCACGCGCAAGATGGTGGAG
TCGTTTACCCGCACAACGATAGGCACGGATGAAGCGCCTATTGACGGGATTCCCCATCAGGGCACCGAAGTCGCGTAG

Upstream 100 bases:

>100_bases
GCCACGGTCACGGCGCCCAACGGCAACAAGGCTTTCCCGAGCAAGCGCCCCTGGATGATCAGTCACTGATCCCAACCCTG
TCAAAACCCATGAGGCCAAC

Downstream 100 bases:

>100_bases
GGCCGGCACCCGGCCAGGGTGATGGTCGGTGCACAGCACACGGTTTGCCCGGAGGTTTGAATTTCCTGAACTGATGAAAG
TGGCTCAGGAATGGCTGCCA

Product: acylamide amidohydrolase

Products: NA

Alternate protein names: Acylamide amidohydrolase [H]

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MIHGDISSSKDTVGVAVVNYKMPRLHSQADVMENARNIARMVEGMKQGLPGLDLVIFPEYSTHGIMYDSKEMHENAATVP
GFETDIFAAACRKAKVWGVFSLTGERHEDHPRKAPYNTLILMNDRGEIVQKYRKIMPWTPVEGWYPGDRTYVSEGPKGLK
VSLIICDDGNYPEIWRDCAMRGAELIVRCQGYMYPAKEQQILISKAMAFANNTYVAVANAAGFDGVYSYFGHSALIGFDG
RTLGECGEEEMGIQYAALSKSLIRDFRRNGQSENHLFKLVHRGYTGVINSGDGDKGVAACPYDFYANWLRDPEGTRKMVE
SFTRTTIGTDEAPIDGIPHQGTEVA

Sequences:

>Translated_345_residues
MIHGDISSSKDTVGVAVVNYKMPRLHSQADVMENARNIARMVEGMKQGLPGLDLVIFPEYSTHGIMYDSKEMHENAATVP
GFETDIFAAACRKAKVWGVFSLTGERHEDHPRKAPYNTLILMNDRGEIVQKYRKIMPWTPVEGWYPGDRTYVSEGPKGLK
VSLIICDDGNYPEIWRDCAMRGAELIVRCQGYMYPAKEQQILISKAMAFANNTYVAVANAAGFDGVYSYFGHSALIGFDG
RTLGECGEEEMGIQYAALSKSLIRDFRRNGQSENHLFKLVHRGYTGVINSGDGDKGVAACPYDFYANWLRDPEGTRKMVE
SFTRTTIGTDEAPIDGIPHQGTEVA
>Mature_345_residues
MIHGDISSSKDTVGVAVVNYKMPRLHSQADVMENARNIARMVEGMKQGLPGLDLVIFPEYSTHGIMYDSKEMHENAATVP
GFETDIFAAACRKAKVWGVFSLTGERHEDHPRKAPYNTLILMNDRGEIVQKYRKIMPWTPVEGWYPGDRTYVSEGPKGLK
VSLIICDDGNYPEIWRDCAMRGAELIVRCQGYMYPAKEQQILISKAMAFANNTYVAVANAAGFDGVYSYFGHSALIGFDG
RTLGECGEEEMGIQYAALSKSLIRDFRRNGQSENHLFKLVHRGYTGVINSGDGDKGVAACPYDFYANWLRDPEGTRKMVE
SFTRTTIGTDEAPIDGIPHQGTEVA

Specific function: Also exhibits in vitro acyl transferase activity, transferring the acyl moiety of short-chain amides to hydroxylamine to form hydroxamates [H]

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6323383, Length=265, Percent_Identity=25.2830188679245, Blast_Score=67, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.4 [H]

Molecular weight: Translated: 38374; Mature: 38374

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHGDISSSKDTVGVAVVNYKMPRLHSQADVMENARNIARMVEGMKQGLPGLDLVIFPEY
CCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCC
STHGIMYDSKEMHENAATVPGFETDIFAAACRKAKVWGVFSLTGERHEDHPRKAPYNTLI
CCCCEEECCHHHHCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCEEE
LMNDRGEIVQKYRKIMPWTPVEGWYPGDRTYVSEGPKGLKVSLIICDDGNYPEIWRDCAM
EECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHH
RGAELIVRCQGYMYPAKEQQILISKAMAFANNTYVAVANAAGFDGVYSYFGHSALIGFDG
CCHHEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCEEEECCC
RTLGECGEEEMGIQYAALSKSLIRDFRRNGQSENHLFKLVHRGYTGVINSGDGDKGVAAC
CCHHHCCCHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEECCCCCCCCEEC
PYDFYANWLRDPEGTRKMVESFTRTTIGTDEAPIDGIPHQGTEVA
CHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIHGDISSSKDTVGVAVVNYKMPRLHSQADVMENARNIARMVEGMKQGLPGLDLVIFPEY
CCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCC
STHGIMYDSKEMHENAATVPGFETDIFAAACRKAKVWGVFSLTGERHEDHPRKAPYNTLI
CCCCEEECCHHHHCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCEEE
LMNDRGEIVQKYRKIMPWTPVEGWYPGDRTYVSEGPKGLKVSLIICDDGNYPEIWRDCAM
EECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHH
RGAELIVRCQGYMYPAKEQQILISKAMAFANNTYVAVANAAGFDGVYSYFGHSALIGFDG
CCHHEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCEEEECCC
RTLGECGEEEMGIQYAALSKSLIRDFRRNGQSENHLFKLVHRGYTGVINSGDGDKGVAAC
CCHHHCCCHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEECCCCCCCCEEC
PYDFYANWLRDPEGTRKMVESFTRTTIGTDEAPIDGIPHQGTEVA
CHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA