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The map label for this gene is pleD [H]

Identifier: 91786468

GI number: 91786468

Start: 561807

End: 562700

Strand: Reverse

Name: pleD [H]

Synonym: Bpro_0562

Alternate gene names: 91786468

Gene position: 562700-561807 (Counterclockwise)

Preceding gene: 91786469

Following gene: 91786467

Centisome position: 10.82

GC content: 65.1

Gene sequence:

>894_bases
ATGCCTGCGAACCCTGACCCGCAGCGCCCCGTCGTCCTGGTGGTCGACGATGCCCCCAGCAGCCTGGGCATGCTCTGCGA
TTCGCTGGAAGGCGAGGGCTACACCGTGCTGGTGGCCAGGGACGGCGACGCTGCGCTGCAGCGTCTTGAATTGGTGGCGC
CCGACGCCATCCTGCTCGATGCCGTCATGCCCGGCCTGTCCGGCTTTGACACCTGCCGGCGCATCAAGGCAAACCCGGCA
CTGGCACACATTCCCGTGATCTTCATGACAGGCCTGTCTGAAACGCCTCATGTGCTGGAAGGCTTTGCCTGCGGCGGTGT
GGACTACGTGGTCAAGCCCCTGCGCGCGCAGGAAGTCGTGGCGCGCCTGCACACCCATACCCGCAACGCCCGCATGACCC
GGCTGGCCCGCGAGGCTGTGGACGTGGCCGGATTCGGTGTGGTGCTGGTGGATGCCAAGGGACGTGTCGCATGGCGCTCA
CCGCAGGCCGCCCTGTGGCTTCAAACGCCCGAAGGTTTGGCGGCAGCTGGTGAGTTGCCCGATCCGGTGCATGCCGCGCT
GGGCGGCAAAACTGATCAGGTACTGATGACCCTGGCCGGCAGGCAACTGTCCGTGCGCAACATGGGCAGGGTCGGCATCG
GGGAGACCATGCTGCTTCTGGAACAGCGTGGCGCCAACGGCATGGCCCCCTCACGCCTGGCCAATGCCGCCTTGACACCG
CGTGAAACCGAAGTCCTGTCATGGCTGGCCAAGGGAAAAACCAACAAAGATATTGGAGACATTCTTGGCATGAGCCACCG
CACGGTGAACAAGCATCTGGAGCACATCTTCGAGAAGCTGGGGGTTGAGACGCGAGCCGCAGCGGCGGCACTGGCCACCG
CGCATGTCGCCTGA

Upstream 100 bases:

>100_bases
GCCGAAGACCCCACACTCGCCGCCAGTTGCGCCAGGCTGCGCGGCTTCGTCAGCCGCTGCGAGCTTGAAGCCCTGCTGGA
TCATTTGACGGAGGAGGAAG

Downstream 100 bases:

>100_bases
ACCGGGGCCTTTTCGCTGAAAGTCTTTTGCTGCAGTGTCTGCGGCATGCCTCCAGCCCTCCTGGGGAATACCGTGTGATC
CGTACGCCAGTCGTCCCATG

Product: two component LuxR family transcriptional regulator

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MPANPDPQRPVVLVVDDAPSSLGMLCDSLEGEGYTVLVARDGDAALQRLELVAPDAILLDAVMPGLSGFDTCRRIKANPA
LAHIPVIFMTGLSETPHVLEGFACGGVDYVVKPLRAQEVVARLHTHTRNARMTRLAREAVDVAGFGVVLVDAKGRVAWRS
PQAALWLQTPEGLAAAGELPDPVHAALGGKTDQVLMTLAGRQLSVRNMGRVGIGETMLLLEQRGANGMAPSRLANAALTP
RETEVLSWLAKGKTNKDIGDILGMSHRTVNKHLEHIFEKLGVETRAAAAALATAHVA

Sequences:

>Translated_297_residues
MPANPDPQRPVVLVVDDAPSSLGMLCDSLEGEGYTVLVARDGDAALQRLELVAPDAILLDAVMPGLSGFDTCRRIKANPA
LAHIPVIFMTGLSETPHVLEGFACGGVDYVVKPLRAQEVVARLHTHTRNARMTRLAREAVDVAGFGVVLVDAKGRVAWRS
PQAALWLQTPEGLAAAGELPDPVHAALGGKTDQVLMTLAGRQLSVRNMGRVGIGETMLLLEQRGANGMAPSRLANAALTP
RETEVLSWLAKGKTNKDIGDILGMSHRTVNKHLEHIFEKLGVETRAAAAALATAHVA
>Mature_296_residues
PANPDPQRPVVLVVDDAPSSLGMLCDSLEGEGYTVLVARDGDAALQRLELVAPDAILLDAVMPGLSGFDTCRRIKANPAL
AHIPVIFMTGLSETPHVLEGFACGGVDYVVKPLRAQEVVARLHTHTRNARMTRLAREAVDVAGFGVVLVDAKGRVAWRSP
QAALWLQTPEGLAAAGELPDPVHAALGGKTDQVLMTLAGRQLSVRNMGRVGIGETMLLLEQRGANGMAPSRLANAALTPR
ETEVLSWLAKGKTNKDIGDILGMSHRTVNKHLEHIFEKLGVETRAAAAALATAHVA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1788394, Length=154, Percent_Identity=30.5194805194805, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790860, Length=159, Percent_Identity=32.7044025157233, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1786599, Length=129, Percent_Identity=34.8837209302326, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1790437, Length=143, Percent_Identity=36.3636363636364, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1788550, Length=108, Percent_Identity=30.5555555555556, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1790299, Length=114, Percent_Identity=35.0877192982456, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 31507; Mature: 31376

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPANPDPQRPVVLVVDDAPSSLGMLCDSLEGEGYTVLVARDGDAALQRLELVAPDAILLD
CCCCCCCCCCEEEEECCCCCHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHCCCCHHHHH
AVMPGLSGFDTCRRIKANPALAHIPVIFMTGLSETPHVLEGFACGGVDYVVKPLRAQEVV
HHHCCCCCHHHHHHHCCCCCHHHHHEEEEECCCCCCHHHHHHCCCCHHHHHCCHHHHHHH
ARLHTHTRNARMTRLAREAVDVAGFGVVLVDAKGRVAWRSPQAALWLQTPEGLAAAGELP
HHHHHHCCHHHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCEEEEECCCCCCCCCCCC
DPVHAALGGKTDQVLMTLAGRQLSVRNMGRVGIGETMLLLEQRGANGMAPSRLANAALTP
CHHHHHHCCCHHHHHHHHCCCEEEHHCCCCCCCCHHEEEEECCCCCCCCHHHHHHCCCCC
RETEVLSWLAKGKTNKDIGDILGMSHRTVNKHLEHIFEKLGVETRAAAAALATAHVA
CHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PANPDPQRPVVLVVDDAPSSLGMLCDSLEGEGYTVLVARDGDAALQRLELVAPDAILLD
CCCCCCCCCEEEEECCCCCHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHCCCCHHHHH
AVMPGLSGFDTCRRIKANPALAHIPVIFMTGLSETPHVLEGFACGGVDYVVKPLRAQEVV
HHHCCCCCHHHHHHHCCCCCHHHHHEEEEECCCCCCHHHHHHCCCCHHHHHCCHHHHHHH
ARLHTHTRNARMTRLAREAVDVAGFGVVLVDAKGRVAWRSPQAALWLQTPEGLAAAGELP
HHHHHHCCHHHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCEEEEECCCCCCCCCCCC
DPVHAALGGKTDQVLMTLAGRQLSVRNMGRVGIGETMLLLEQRGANGMAPSRLANAALTP
CHHHHHHCCCHHHHHHHHCCCEEEHHCCCCCCCCHHEEEEECCCCCCCCHHHHHHCCCCC
RETEVLSWLAKGKTNKDIGDILGMSHRTVNKHLEHIFEKLGVETRAAAAALATAHVA
CHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA