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The map label for this gene is hyfR [C]

Identifier: 91786441

GI number: 91786441

Start: 532375

End: 533799

Strand: Direct

Name: hyfR [C]

Synonym: Bpro_0533

Alternate gene names: 91786441

Gene position: 532375-533799 (Clockwise)

Preceding gene: 91786436

Following gene: 91786443

Centisome position: 10.24

GC content: 55.65

Gene sequence:

>1425_bases
ATGCAGACTCACGAGAACATATCCACGTCCGATTCCTTGTCGAGCAAGACCGAAATCAGCGAGTTGCTGCATTTGCGACA
GCTTTGCAAAGACTTCGAGGAAATTTATCGCAACTCGTTTGACGGCATTTTTGTGGCCGATGGCAAGGGCATCACGCTGA
TGGTCAACGAGGGCTGTGAGCGCAACTACGACCTCAAGGCTGAGGAATTGGTAGGTCACCACGTTTCAGAATTTGAAAAA
AAGGGGTGGATACGTCCGGTGATTGCAACCCGTGTGGCGGCCGAGCGAAAACGCATTTCTGCCATGCAGCACACCCACAC
TGGGAAAACCATCATGGTCACGGGCATTCCCTTGTTCGACGACCAGGGCGCGGTGCGCAAAGTCATCATCAACTCGCGCG
ACACCACCGAGCTGGTGCAGTTGCAGGAGGCATTGCTGCGCACGCGCGAGGATTTGCAGCGTGTTGAAAACGAGATGAAA
GAGCTACGTCTGCAAAACCTCAAACCCGATGGTGTGGCGCTCAAAAGCCAGAGCATGCAGCGCTTGTCCAGCTTGGCATT
GCGAGTAGCCAAAGTGGATACGGCGGTGCTGATCAGCGGCGAATCGGGGGTGGGCAAGGAGGTGCTGGCGCGACAGATTC
ACAAGGAAAGTCAGCGTAGCCAGGGACCTTTGATCAAAATCAATTGCGGCGCCATTCCACGCGAGTTGCTGGAGTCGGAA
TTGTTTGGCTACGAGGGAGGGGCGTTCACTGGCGCACTCAAGCAGGGCAAGGTAGGCCTGATAGAAACCGCCCACCACGG
CACCCTGTTTCTGGATGAGATCGGCGAACTGCCGATGAACCTGCAGGTCAAGCTGCTCCAGACGCTGCAAGACCATACTG
TGGTGCGTGTGGGCGGCACTCGGGTCGTCCACGTCGATCTGCGGGTGATTGCGGCAACCAATCGCGACTTGCAAGTCATG
GTGGACAACAACCAGTTTCGCAGCGACCTGTACTACCGCCTGAACGTGGTTCCATTGAGCGTGCCGCCCCTGCGCTTGCG
CAAGGAAGACATTTTTCCGCTGACAGAGCATGCGCTCGACGAGTTAAATGCCTCATACAGTTTCAACAAGCGATTTTCCG
AACGCGCCTTGAAGTCTTTGCTGACGTACGAGTGGCCTGGCAATGTGCGCGAGCTGCGCAATATCGTCGAGCGCCTGGTG
GTGACCACGCCTACGGACCTGATTGACGTGATCGACTTGCCGGAATATCTAATAGCACCAGTGGACCAGGCCTTCGCAGA
CCCGCTTGAGTACCGCGCCAAAGTGGCTCAATTTGAAAAGCAACTTGTCGCAGAGGCGCTCAAGAAATATGGCAATACAC
GCGCCGTGGCCAAGCAGTTAAATCTCAGCCAATCCACTGTGGTTCGCAAGCTGAACGCAGCATGA

Upstream 100 bases:

>100_bases
GTTTTGGCCTATGGCGATTTGCTTCGCGTGGCTCTTGCCCTATGCTTTGCGGGGCTTGATTCAATTTTGAATCAAGCCGC
ACCGAGCAAGGCAACCCACC

Downstream 100 bases:

>100_bases
CAAGTTATTTGCGGTCGCCCAAGACTATTCTCCAGAATATCGGGGGGGGTGATGCTACGGCTGATTCAGGCCGTGTGTAT
TGGTTGCGCAAAACCACCCA

Product: sigma-54 dependent trancsriptional regulator

Products: NA

Alternate protein names: ORF1 [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MQTHENISTSDSLSSKTEISELLHLRQLCKDFEEIYRNSFDGIFVADGKGITLMVNEGCERNYDLKAEELVGHHVSEFEK
KGWIRPVIATRVAAERKRISAMQHTHTGKTIMVTGIPLFDDQGAVRKVIINSRDTTELVQLQEALLRTREDLQRVENEMK
ELRLQNLKPDGVALKSQSMQRLSSLALRVAKVDTAVLISGESGVGKEVLARQIHKESQRSQGPLIKINCGAIPRELLESE
LFGYEGGAFTGALKQGKVGLIETAHHGTLFLDEIGELPMNLQVKLLQTLQDHTVVRVGGTRVVHVDLRVIAATNRDLQVM
VDNNQFRSDLYYRLNVVPLSVPPLRLRKEDIFPLTEHALDELNASYSFNKRFSERALKSLLTYEWPGNVRELRNIVERLV
VTTPTDLIDVIDLPEYLIAPVDQAFADPLEYRAKVAQFEKQLVAEALKKYGNTRAVAKQLNLSQSTVVRKLNAA

Sequences:

>Translated_474_residues
MQTHENISTSDSLSSKTEISELLHLRQLCKDFEEIYRNSFDGIFVADGKGITLMVNEGCERNYDLKAEELVGHHVSEFEK
KGWIRPVIATRVAAERKRISAMQHTHTGKTIMVTGIPLFDDQGAVRKVIINSRDTTELVQLQEALLRTREDLQRVENEMK
ELRLQNLKPDGVALKSQSMQRLSSLALRVAKVDTAVLISGESGVGKEVLARQIHKESQRSQGPLIKINCGAIPRELLESE
LFGYEGGAFTGALKQGKVGLIETAHHGTLFLDEIGELPMNLQVKLLQTLQDHTVVRVGGTRVVHVDLRVIAATNRDLQVM
VDNNQFRSDLYYRLNVVPLSVPPLRLRKEDIFPLTEHALDELNASYSFNKRFSERALKSLLTYEWPGNVRELRNIVERLV
VTTPTDLIDVIDLPEYLIAPVDQAFADPLEYRAKVAQFEKQLVAEALKKYGNTRAVAKQLNLSQSTVVRKLNAA
>Mature_474_residues
MQTHENISTSDSLSSKTEISELLHLRQLCKDFEEIYRNSFDGIFVADGKGITLMVNEGCERNYDLKAEELVGHHVSEFEK
KGWIRPVIATRVAAERKRISAMQHTHTGKTIMVTGIPLFDDQGAVRKVIINSRDTTELVQLQEALLRTREDLQRVENEMK
ELRLQNLKPDGVALKSQSMQRLSSLALRVAKVDTAVLISGESGVGKEVLARQIHKESQRSQGPLIKINCGAIPRELLESE
LFGYEGGAFTGALKQGKVGLIETAHHGTLFLDEIGELPMNLQVKLLQTLQDHTVVRVGGTRVVHVDLRVIAATNRDLQVM
VDNNQFRSDLYYRLNVVPLSVPPLRLRKEDIFPLTEHALDELNASYSFNKRFSERALKSLLTYEWPGNVRELRNIVERLV
VTTPTDLIDVIDLPEYLIAPVDQAFADPLEYRAKVAQFEKQLVAEALKKYGNTRAVAKQLNLSQSTVVRKLNAA

Specific function: Required For Induction Of Expression Of The Hydrogenase- 4 Structural Genes. [C]

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082117, Length=258, Percent_Identity=51.1627906976744, Blast_Score=250, Evalue=2e-67,
Organism=Escherichia coli, GI1788550, Length=309, Percent_Identity=45.6310679611651, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI1789233, Length=309, Percent_Identity=41.4239482200647, Blast_Score=235, Evalue=4e-63,
Organism=Escherichia coli, GI1790437, Length=305, Percent_Identity=43.9344262295082, Blast_Score=230, Evalue=2e-61,
Organism=Escherichia coli, GI1788905, Length=240, Percent_Identity=47.9166666666667, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI1786524, Length=315, Percent_Identity=41.2698412698413, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1789087, Length=312, Percent_Identity=41.025641025641, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI1790299, Length=327, Percent_Identity=37.9204892966361, Blast_Score=198, Evalue=5e-52,
Organism=Escherichia coli, GI87082152, Length=214, Percent_Identity=48.5981308411215, Blast_Score=196, Evalue=4e-51,
Organism=Escherichia coli, GI87081872, Length=284, Percent_Identity=41.9014084507042, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1787583, Length=325, Percent_Identity=36, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI87081858, Length=295, Percent_Identity=33.5593220338983, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI1789828, Length=279, Percent_Identity=34.4086021505376, Blast_Score=141, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR002078 [H]

Pfam domain/function: PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 53348; Mature: 53348

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTHENISTSDSLSSKTEISELLHLRQLCKDFEEIYRNSFDGIFVADGKGITLMVNEGCE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCC
RNYDLKAEELVGHHVSEFEKKGWIRPVIATRVAAERKRISAMQHTHTGKTIMVTGIPLFD
CCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECEEEC
DQGAVRKVIINSRDTTELVQLQEALLRTREDLQRVENEMKELRLQNLKPDGVALKSQSMQ
CCCCHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
RLSSLALRVAKVDTAVLISGESGVGKEVLARQIHKESQRSQGPLIKINCGAIPRELLESE
HHHHHHHHHHEECEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHH
LFGYEGGAFTGALKQGKVGLIETAHHGTLFLDEIGELPMNLQVKLLQTLQDHTVVRVGGT
HCCCCCCCEECCCCCCCEEEEEECCCCEEEHHHHCCCCCCHHHHHHHHHHHCCEEEECCE
RVVHVDLRVIAATNRDLQVMVDNNQFRSDLYYRLNVVPLSVPPLRLRKEDIFPLTEHALD
EEEEEEEEEEEECCCCEEEEECCCCHHCCEEEEEEEEEECCCCCHHCCCCCCCHHHHHHH
ELNASYSFNKRFSERALKSLLTYEWPGNVRELRNIVERLVVTTPTDLIDVIDLPEYLIAP
HHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHC
VDQAFADPLEYRAKVAQFEKQLVAEALKKYGNTRAVAKQLNLSQSTVVRKLNAA
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHCCC
>Mature Secondary Structure
MQTHENISTSDSLSSKTEISELLHLRQLCKDFEEIYRNSFDGIFVADGKGITLMVNEGCE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCC
RNYDLKAEELVGHHVSEFEKKGWIRPVIATRVAAERKRISAMQHTHTGKTIMVTGIPLFD
CCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECEEEC
DQGAVRKVIINSRDTTELVQLQEALLRTREDLQRVENEMKELRLQNLKPDGVALKSQSMQ
CCCCHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
RLSSLALRVAKVDTAVLISGESGVGKEVLARQIHKESQRSQGPLIKINCGAIPRELLESE
HHHHHHHHHHEECEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHH
LFGYEGGAFTGALKQGKVGLIETAHHGTLFLDEIGELPMNLQVKLLQTLQDHTVVRVGGT
HCCCCCCCEECCCCCCCEEEEEECCCCEEEHHHHCCCCCCHHHHHHHHHHHCCEEEECCE
RVVHVDLRVIAATNRDLQVMVDNNQFRSDLYYRLNVVPLSVPPLRLRKEDIFPLTEHALD
EEEEEEEEEEEECCCCEEEEECCCCHHCCEEEEEEEEEECCCCCHHCCCCCCCHHHHHHH
ELNASYSFNKRFSERALKSLLTYEWPGNVRELRNIVERLVVTTPTDLIDVIDLPEYLIAP
HHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHC
VDQAFADPLEYRAKVAQFEKQLVAEALKKYGNTRAVAKQLNLSQSTVVRKLNAA
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1732229 [H]