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The map label for this gene is gatA [H]

Identifier: 91786295

GI number: 91786295

Start: 398447

End: 399631

Strand: Direct

Name: gatA [H]

Synonym: Bpro_0385

Alternate gene names: 91786295

Gene position: 398447-399631 (Clockwise)

Preceding gene: 91786294

Following gene: 91786298

Centisome position: 7.66

GC content: 65.91

Gene sequence:

>1185_bases
ATGCCATTACACGACCCTGCCCATGCTTTTGTTCCCTACCCGGACGTTCCGGTACCCAGCGCTTTCACCGGCCCGTTGGC
CGATCTGCGTTTTGGCGTGAAAGACCTGTTCGATGTGGCGGGTTACCCGACGGGCGGGGGCAGTCCCACCGTGCTTGCGC
TGTCTGGCATCAAGACACGCACCGCACCGACCGTGCAAAAGCTGCTGGACGCCGGCGCACTTTTTGCAGGAAAAACCGTG
ACCGACGAGCTTGCTTTCTCGATGAACGGCAACAATGCTCATTTTGGCGCGCCCCTCAACGGCGCAGCCCATGACCGCAT
CACTGGCGGGTCATCGTCGGGCTCGGCCTCGGCCGTCTCTTCAGGTTTGTGCGACTTTGCGCTCGGCACCGACACCGGTG
GTTCGGTGCGCGCGCCGGCCAATCACTGCGGCCTGTACGGCCTGCGGCCAACCCACGGGCGCGTCAGCCTCGAAAGCGTG
CTCGACCTGGCGCCCAGCCTGGACACCTGCGGCTGGTTTGCGCGAGACGTGGGCACCTTTGTGCGAGTGGGCGACGTCTT
GCTGGGCGAGGACGTCACGCCGCTGCCGGCCGATGTGCGTCTGCTTTGGCCGGAAGACGTGTGGGCGATGATGGATGCGC
CTGCCCGCCGGGTGCTTGAAAGCGGCGCTGCGCAGAGCGTGCAGTCGCTTTTAGGGCCGACCACACCGGTGCAGGTGGTG
CGGGAGTCGTGGGACGCCATGTACTGGAACTTTCGCTACGTGCAGTCGCGCGAGGCCTGGCTGACCGACGGTCCCTTGAT
CGAGCGCTATGCACCCTCACTTGGCCCCGGCGTGGCCGAGCGCTTTGCCTGGTCCTGCAATGTCACTGACGAGCAGGTCA
ACGCCGCGCGCGCTTTTCGCGCCGCGTTCAGGGCGCAGCTTGCAGGGCTGCTGGGCGATGACGGCGTGCTGTTGATGCCC
ACCATGCCTGACATCGCGCCCCTGCGCAGTGCAAGCGAGGCAAGCCTGGAGGACTATCGCAACCGCGCGATCCGCATGCT
TTGCATCGCAGGCCTGGCGGGCTTCCCACAACTGAGCATGCCCATGGCCAGGCATGAAGGCGCGCCCCTGGGCCTGTCGC
TGTTGGGCCCGGCTGGCCGTGATCGCTCATTGCTGGCACTGGCGGTGCGCATCGCCTCTTCCTGA

Upstream 100 bases:

>100_bases
CCGGCGCCGATGCGCCCTGTGATGCTTTCCTGAGTCACGCATTGCTCGTTGTGCTCTTTGCGCATTGATTTTTATCCCAA
CCAGATTGACGGCTCGGCCC

Downstream 100 bases:

>100_bases
GGGTGTCAATCTTTAATTGTTCCGTCTTGGGCGCCTGGCAGCTCAAGCCATACAGACGAGGGCAGCGCAGCGACTTCGCA
GTCAACAGCGCTTTCGTGTG

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MPLHDPAHAFVPYPDVPVPSAFTGPLADLRFGVKDLFDVAGYPTGGGSPTVLALSGIKTRTAPTVQKLLDAGALFAGKTV
TDELAFSMNGNNAHFGAPLNGAAHDRITGGSSSGSASAVSSGLCDFALGTDTGGSVRAPANHCGLYGLRPTHGRVSLESV
LDLAPSLDTCGWFARDVGTFVRVGDVLLGEDVTPLPADVRLLWPEDVWAMMDAPARRVLESGAAQSVQSLLGPTTPVQVV
RESWDAMYWNFRYVQSREAWLTDGPLIERYAPSLGPGVAERFAWSCNVTDEQVNAARAFRAAFRAQLAGLLGDDGVLLMP
TMPDIAPLRSASEASLEDYRNRAIRMLCIAGLAGFPQLSMPMARHEGAPLGLSLLGPAGRDRSLLALAVRIASS

Sequences:

>Translated_394_residues
MPLHDPAHAFVPYPDVPVPSAFTGPLADLRFGVKDLFDVAGYPTGGGSPTVLALSGIKTRTAPTVQKLLDAGALFAGKTV
TDELAFSMNGNNAHFGAPLNGAAHDRITGGSSSGSASAVSSGLCDFALGTDTGGSVRAPANHCGLYGLRPTHGRVSLESV
LDLAPSLDTCGWFARDVGTFVRVGDVLLGEDVTPLPADVRLLWPEDVWAMMDAPARRVLESGAAQSVQSLLGPTTPVQVV
RESWDAMYWNFRYVQSREAWLTDGPLIERYAPSLGPGVAERFAWSCNVTDEQVNAARAFRAAFRAQLAGLLGDDGVLLMP
TMPDIAPLRSASEASLEDYRNRAIRMLCIAGLAGFPQLSMPMARHEGAPLGLSLLGPAGRDRSLLALAVRIASS
>Mature_393_residues
PLHDPAHAFVPYPDVPVPSAFTGPLADLRFGVKDLFDVAGYPTGGGSPTVLALSGIKTRTAPTVQKLLDAGALFAGKTVT
DELAFSMNGNNAHFGAPLNGAAHDRITGGSSSGSASAVSSGLCDFALGTDTGGSVRAPANHCGLYGLRPTHGRVSLESVL
DLAPSLDTCGWFARDVGTFVRVGDVLLGEDVTPLPADVRLLWPEDVWAMMDAPARRVLESGAAQSVQSLLGPTTPVQVVR
ESWDAMYWNFRYVQSREAWLTDGPLIERYAPSLGPGVAERFAWSCNVTDEQVNAARAFRAAFRAQLAGLLGDDGVLLMPT
MPDIAPLRSASEASLEDYRNRAIRMLCIAGLAGFPQLSMPMARHEGAPLGLSLLGPAGRDRSLLALAVRIASS

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=440, Percent_Identity=28.1818181818182, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17543272, Length=407, Percent_Identity=26.7813267813268, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17556264, Length=172, Percent_Identity=33.1395348837209, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=428, Percent_Identity=27.5700934579439, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6319685, Length=394, Percent_Identity=27.1573604060914, Blast_Score=79, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24648113, Length=176, Percent_Identity=38.0681818181818, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21356731, Length=409, Percent_Identity=24.9388753056235, Blast_Score=75, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 41523; Mature: 41391

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLHDPAHAFVPYPDVPVPSAFTGPLADLRFGVKDLFDVAGYPTGGGSPTVLALSGIKTR
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCC
TAPTVQKLLDAGALFAGKTVTDELAFSMNGNNAHFGAPLNGAAHDRITGGSSSGSASAVS
CHHHHHHHHHCCCHHCCCCHHHHHEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
SGLCDFALGTDTGGSVRAPANHCGLYGLRPTHGRVSLESVLDLAPSLDTCGWFARDVGTF
CCCHHEEECCCCCCCEECCHHHCCCCCCCCCCCCEEHHHHHHHCCCCCHHHHHHHHHHHH
VRVGDVLLGEDVTPLPADVRLLWPEDVWAMMDAPARRVLESGAAQSVQSLLGPTTPVQVV
HHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHH
RESWDAMYWNFRYVQSREAWLTDGPLIERYAPSLGPGVAERFAWSCNVTDEQVNAARAFR
HHHHHHHEEEEEEEECCCCCCCCCCHHHHHCCCCCCCHHHHHEEECCCCHHHHHHHHHHH
AAFRAQLAGLLGDDGVLLMPTMPDIAPLRSASEASLEDYRNRAIRMLCIAGLAGFPQLSM
HHHHHHHHHHCCCCCEEEECCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PMARHEGAPLGLSLLGPAGRDRSLLALAVRIASS
CHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
PLHDPAHAFVPYPDVPVPSAFTGPLADLRFGVKDLFDVAGYPTGGGSPTVLALSGIKTR
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCC
TAPTVQKLLDAGALFAGKTVTDELAFSMNGNNAHFGAPLNGAAHDRITGGSSSGSASAVS
CHHHHHHHHHCCCHHCCCCHHHHHEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
SGLCDFALGTDTGGSVRAPANHCGLYGLRPTHGRVSLESVLDLAPSLDTCGWFARDVGTF
CCCHHEEECCCCCCCEECCHHHCCCCCCCCCCCCEEHHHHHHHCCCCCHHHHHHHHHHHH
VRVGDVLLGEDVTPLPADVRLLWPEDVWAMMDAPARRVLESGAAQSVQSLLGPTTPVQVV
HHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHH
RESWDAMYWNFRYVQSREAWLTDGPLIERYAPSLGPGVAERFAWSCNVTDEQVNAARAFR
HHHHHHHEEEEEEEECCCCCCCCCCHHHHHCCCCCCCHHHHHEEECCCCHHHHHHHHHHH
AAFRAQLAGLLGDDGVLLMPTMPDIAPLRSASEASLEDYRNRAIRMLCIAGLAGFPQLSM
HHHHHHHHHHCCCCCEEEECCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PMARHEGAPLGLSLLGPAGRDRSLLALAVRIASS
CHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]