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The map label for this gene is cynS

Identifier: 91786200

GI number: 91786200

Start: 304250

End: 304693

Strand: Reverse

Name: cynS

Synonym: Bpro_0289

Alternate gene names: 91786200

Gene position: 304693-304250 (Counterclockwise)

Preceding gene: 91786201

Following gene: 91786192

Centisome position: 5.86

GC content: 57.43

Gene sequence:

>444_bases
ATGAACCGCAACGACATCACCGAAAAAATCATCACCACCAAGGTTGCCAAGGGCATCACCTGGGAATCGGTCGCCAAAAA
GGTGGGCCTCAGCAAGGAATGGGTCACCGCCGGCTGCCTGGGCCAGATGACCTTTGACGAGAAACAGGCCAAAGTCGTCG
GCAAGATTTTTGGCCTAACAGCCGAGGAGCAAAAGTGGCTGCAGGTCGTGCCGTACAAGGGCTCGCTGCCAACGCCCGTG
CCCACCGACCCGCTGATCTACCGCTGGTACGAGATCGTCAGCGTCTATGGCACCACCATCAAGGAACTGATTCACGAAGA
GTTCGGCGACGGCATCATGAGCGCCATCGACTTCAGCATGGACATCGTGCGCCAGCCCGACCCAAAGGGCGACCGTGTCA
ACGTGGTGCTGTCGGGAAAGTTTTTGCCGTACAAGACCTATTGA

Upstream 100 bases:

>100_bases
TCGATTTTCTCGTCATGCGCGCCAAGGCGCATTGACGCCCGACCCGAACACCACCCTTCACAACCGCCTCAAAACCACTT
CAAACACAGGAGCCATAGAC

Downstream 100 bases:

>100_bases
CCCTGTCAGCGGAGCCGCAGCCGGCGCGTTGCAATTTTCCGACGGGCCGCTCCCAAGAAAAATTAGCCCCCTCGGGGGCA
GCGTTTGCACGCAGTGAAAA

Product: cyanate hydratase

Products: NA

Alternate protein names: Cyanase; Cyanate hydrolase; Cyanate lyase

Number of amino acids: Translated: 147; Mature: 147

Protein sequence:

>147_residues
MNRNDITEKIITTKVAKGITWESVAKKVGLSKEWVTAGCLGQMTFDEKQAKVVGKIFGLTAEEQKWLQVVPYKGSLPTPV
PTDPLIYRWYEIVSVYGTTIKELIHEEFGDGIMSAIDFSMDIVRQPDPKGDRVNVVLSGKFLPYKTY

Sequences:

>Translated_147_residues
MNRNDITEKIITTKVAKGITWESVAKKVGLSKEWVTAGCLGQMTFDEKQAKVVGKIFGLTAEEQKWLQVVPYKGSLPTPV
PTDPLIYRWYEIVSVYGTTIKELIHEEFGDGIMSAIDFSMDIVRQPDPKGDRVNVVLSGKFLPYKTY
>Mature_147_residues
MNRNDITEKIITTKVAKGITWESVAKKVGLSKEWVTAGCLGQMTFDEKQAKVVGKIFGLTAEEQKWLQVVPYKGSLPTPV
PTDPLIYRWYEIVSVYGTTIKELIHEEFGDGIMSAIDFSMDIVRQPDPKGDRVNVVLSGKFLPYKTY

Specific function: Catalyzes the reaction of cyanate with bicarbonate to produce ammonia and carbon dioxide

COG id: COG1513

COG function: function code P; Cyanate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cyanase family

Homologues:

Organism=Escherichia coli, GI1786535, Length=151, Percent_Identity=40.3973509933775, Blast_Score=134, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYNS_POLSJ (Q12GT8)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_547152.1
- ProteinModelPortal:   Q12GT8
- SMR:   Q12GT8
- STRING:   Q12GT8
- GeneID:   4011660
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_0289
- NMPDR:   fig|296591.1.peg.3548
- eggNOG:   COG1513
- HOGENOM:   HBG317643
- OMA:   SAIDFDM
- ProtClustDB:   PRK02866
- BioCyc:   PSP296591:BPRO_0289-MONOMER
- HAMAP:   MF_00535
- InterPro:   IPR008076
- InterPro:   IPR003712
- InterPro:   IPR010982
- Gene3D:   G3DSA:3.30.1160.10
- PIRSF:   PIRSF001263
- PRINTS:   PR01693
- TIGRFAMs:   TIGR00673

Pfam domain/function: PF02560 Cyanate_lyase; SSF55234 Cyanate_lyase_C; SSF47413 Lambda_like_DNA

EC number: =4.2.1.104

Molecular weight: Translated: 16559; Mature: 16559

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: NA

Important sites: ACT_SITE 88-88 ACT_SITE 91-91 ACT_SITE 114-114

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRNDITEKIITTKVAKGITWESVAKKVGLSKEWVTAGCLGQMTFDEKQAKVVGKIFGLT
CCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
AEEQKWLQVVPYKGSLPTPVPTDPLIYRWYEIVSVYGTTIKELIHEEFGDGIMSAIDFSM
CCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
DIVRQPDPKGDRVNVVLSGKFLPYKTY
HHHCCCCCCCCEEEEEEECCCCCCCCC
>Mature Secondary Structure
MNRNDITEKIITTKVAKGITWESVAKKVGLSKEWVTAGCLGQMTFDEKQAKVVGKIFGLT
CCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
AEEQKWLQVVPYKGSLPTPVPTDPLIYRWYEIVSVYGTTIKELIHEEFGDGIMSAIDFSM
CCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
DIVRQPDPKGDRVNVVLSGKFLPYKTY
HHHCCCCCCCCEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA