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The map label for this gene is exoA [H]

Identifier: 91786127

GI number: 91786127

Start: 236221

End: 237270

Strand: Reverse

Name: exoA [H]

Synonym: Bpro_0216

Alternate gene names: 91786127

Gene position: 237270-236221 (Counterclockwise)

Preceding gene: 91786130

Following gene: 91786126

Centisome position: 4.56

GC content: 63.05

Gene sequence:

>1050_bases
ATGTCCTTGTTCAAATTAACCAGCCTCAACCTCAACGGTATCCGTTCCGCTACCAGCAAAGGCCTGGAGGACTGGATTGC
CCAGTCCCTACCGGATTGTATTTGCGTACAGGAGCTGAAGGCGCAAGCGACTGATGTGAGTGGTCGCTTCGAAGCATTGG
CCGGTCTCAAGGGCTACTTTCACTTTGCCGAGAAGAAAGGCTATTCGGGCGTGGCGGTGTACACCCGCCATGAGCCCAGC
GACGTCATTGTGGGCTATGGCTCGTCCGAATTTGACGCCGAAGGCCGCTACGTGGAACTGCGCTTTGACCGGCCGGGCCG
CAAGCACGCGCCCAAGCTGTCCATCATCAGCTGCTACTTCCCCAGCGGCTCATCGGGCGAAGAGCGGCAGGCCGCCAAGT
TCCGCTTTCTGGCCGAGTTCTACCCGCGCCTGCTGGCGCTGAAGGAAACGCGCGACTTTGTGCTGTGCGGCGACGTCAAC
ATCGCGCACCAGGAAATCGATCTGAAAAACTTCAAGGGCAACCGCAAGAACAGCGGCTTCCTGCCCGAAGAGCGCGCCTG
GATGACGGAGCTGCTGAAGGACGCCGAGCTGGCCAATGAAGCGGCGGACGAGCTTAACGCCCCCACGCTCGGCACTGGCG
TGTCCTCGCTGCCCCCCAAGGGGGCTGAACTTGCTCGGGGCGGCCCTTCGCTGCATTCGGCCCCCACGCTCGGCACTGGC
GTGTCCTCGCTGCCCCCCGAGGGGGCTGAACTTGCTCGGGGCGGCCCTTCGCTGCGTTCGTCAGTACCCACAGCGGGAAC
TGGCGCGGCGCCACCGCTCGGTGGTGGCATGGTCGATGTCTACCGCAAGCTGCAACCCACCACCACCGACGCGGCCTACA
CCTGGTGGAGCAATCGCGGCCAGGCCTATGCCAAGAACGTGGGCTGGCGGCTCGACTACCACCTGGCCACGCCCGCCTTT
GCGGCACTGGCGCGGTCTGAGCATATTTACAGGGCCGAGCGTTTTTCGGACCATGCGCCGATCACGGTGGATTACGACTG
GTCTGTTTGA

Upstream 100 bases:

>100_bases
TCCAGCGCAAACTGCACGAATTCCTGTGCCAGCGTGGTGTCTGCGGGAGTTGCCTGCGCCTTGTCCGCTTTGTCTGTCTG
CCCTGCCGTGCTCATTGGAG

Downstream 100 bases:

>100_bases
GGGGCAGGCTAAACTGCCGGAGATACGGCTTGCCCGCCCAGGTCGAGCGCAGAGTTGTGCCCGATTCAACCGTTCTTTAA
ACACCTGTCGCCCCTGTGAA

Product: exodeoxyribonuclease III (xth)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MSLFKLTSLNLNGIRSATSKGLEDWIAQSLPDCICVQELKAQATDVSGRFEALAGLKGYFHFAEKKGYSGVAVYTRHEPS
DVIVGYGSSEFDAEGRYVELRFDRPGRKHAPKLSIISCYFPSGSSGEERQAAKFRFLAEFYPRLLALKETRDFVLCGDVN
IAHQEIDLKNFKGNRKNSGFLPEERAWMTELLKDAELANEAADELNAPTLGTGVSSLPPKGAELARGGPSLHSAPTLGTG
VSSLPPEGAELARGGPSLRSSVPTAGTGAAPPLGGGMVDVYRKLQPTTTDAAYTWWSNRGQAYAKNVGWRLDYHLATPAF
AALARSEHIYRAERFSDHAPITVDYDWSV

Sequences:

>Translated_349_residues
MSLFKLTSLNLNGIRSATSKGLEDWIAQSLPDCICVQELKAQATDVSGRFEALAGLKGYFHFAEKKGYSGVAVYTRHEPS
DVIVGYGSSEFDAEGRYVELRFDRPGRKHAPKLSIISCYFPSGSSGEERQAAKFRFLAEFYPRLLALKETRDFVLCGDVN
IAHQEIDLKNFKGNRKNSGFLPEERAWMTELLKDAELANEAADELNAPTLGTGVSSLPPKGAELARGGPSLHSAPTLGTG
VSSLPPEGAELARGGPSLRSSVPTAGTGAAPPLGGGMVDVYRKLQPTTTDAAYTWWSNRGQAYAKNVGWRLDYHLATPAF
AALARSEHIYRAERFSDHAPITVDYDWSV
>Mature_348_residues
SLFKLTSLNLNGIRSATSKGLEDWIAQSLPDCICVQELKAQATDVSGRFEALAGLKGYFHFAEKKGYSGVAVYTRHEPSD
VIVGYGSSEFDAEGRYVELRFDRPGRKHAPKLSIISCYFPSGSSGEERQAAKFRFLAEFYPRLLALKETRDFVLCGDVNI
AHQEIDLKNFKGNRKNSGFLPEERAWMTELLKDAELANEAADELNAPTLGTGVSSLPPKGAELARGGPSLHSAPTLGTGV
SSLPPEGAELARGGPSLRSSVPTAGTGAAPPLGGGMVDVYRKLQPTTTDAAYTWWSNRGQAYAKNVGWRLDYHLATPAFA
ALARSEHIYRAERFSDHAPITVDYDWSV

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=198, Percent_Identity=36.3636363636364, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI18375503, Length=198, Percent_Identity=36.3636363636364, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI18375501, Length=198, Percent_Identity=36.3636363636364, Blast_Score=132, Evalue=4e-31,
Organism=Escherichia coli, GI1788046, Length=204, Percent_Identity=31.8627450980392, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71989536, Length=194, Percent_Identity=27.8350515463918, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI221330655, Length=189, Percent_Identity=32.8042328042328, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI17136678, Length=189, Percent_Identity=32.8042328042328, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 37964; Mature: 37833

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLFKLTSLNLNGIRSATSKGLEDWIAQSLPDCICVQELKAQATDVSGRFEALAGLKGYF
CCCEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
HFAEKKGYSGVAVYTRHEPSDVIVGYGSSEFDAEGRYVELRFDRPGRKHAPKLSIISCYF
HHHHHCCCCCEEEEEECCCCCEEEECCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEC
PSGSSGEERQAAKFRFLAEFYPRLLALKETRDFVLCGDVNIAHQEIDLKNFKGNRKNSGF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHEECHHHCCCCCCCCCC
LPEERAWMTELLKDAELANEAADELNAPTLGTGVSSLPPKGAELARGGPSLHSAPTLGTG
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCC
VSSLPPEGAELARGGPSLRSSVPTAGTGAAPPLGGGMVDVYRKLQPTTTDAAYTWWSNRG
CCCCCCCCHHHHCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCEEEEECCCC
QAYAKNVGWRLDYHLATPAFAALARSEHIYRAERFSDHAPITVDYDWSV
CEEHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCC
>Mature Secondary Structure 
SLFKLTSLNLNGIRSATSKGLEDWIAQSLPDCICVQELKAQATDVSGRFEALAGLKGYF
CCEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
HFAEKKGYSGVAVYTRHEPSDVIVGYGSSEFDAEGRYVELRFDRPGRKHAPKLSIISCYF
HHHHHCCCCCEEEEEECCCCCEEEECCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEC
PSGSSGEERQAAKFRFLAEFYPRLLALKETRDFVLCGDVNIAHQEIDLKNFKGNRKNSGF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHEECHHHCCCCCCCCCC
LPEERAWMTELLKDAELANEAADELNAPTLGTGVSSLPPKGAELARGGPSLHSAPTLGTG
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCC
VSSLPPEGAELARGGPSLRSSVPTAGTGAAPPLGGGMVDVYRKLQPTTTDAAYTWWSNRG
CCCCCCCCHHHHCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCEEEEECCCC
QAYAKNVGWRLDYHLATPAFAALARSEHIYRAERFSDHAPITVDYDWSV
CEEHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]