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The map label for this gene is livK [C]

Identifier: 91786061

GI number: 91786061

Start: 156670

End: 157923

Strand: Direct

Name: livK [C]

Synonym: Bpro_0150

Alternate gene names: 91786061

Gene position: 156670-157923 (Clockwise)

Preceding gene: 91786060

Following gene: 91786062

Centisome position: 3.01

GC content: 61.8

Gene sequence:

>1254_bases
ATGTTCATCAACTCACGTCTTGCCGGCTTCGGCGTTGCCGGTGCCGTTGCCGCCATCGTGGCGCTGGGCACCGCGCTGCC
GGCGGCAGCCCAGGGGGTTTCCGCTGCGGCGGCACCTCCGTCCCCGGCCGCCAACACGCTCAAGATTGGCGTGATAGGTC
CCTTCACCGGTGCGTCATCCGACTTCGGCGTGCCCATGCTCAACGGCATCCAGCAGGCTGTGGACGAGATCAATGCGGTG
GGCGGCTACCTGGGCCGCAAGCTGGAGATTGTGCGCAAGGACGACAAGGCCAACCCCGATGTGGGCCTGAAAATGTCGCA
GGAACTGGTCGCCGAAAAAGTGTCTGTAACCCTTGGCTTTTGCAATACCGGCGTGGCCGCCAAGTCGCTGGAGGTCTACC
AGGCCAACAAGCTGCCACTGATCATTCCGTGCGCCACCGGCTCGCCGCTCACGGCGAAATACCCCGCAGCGGAGAGCTAT
ATCTTTCGCACGTCGGCCAGCGACAGCATCCAGGCCCCGTTCGTCGTGGAGGACATCCTCAAGCGCGGCTGGAACAGGGT
GGCGGTGTTTGCCGACACCACCGGGTATGGCGAAGCCGGCCTGCAGGATGTGGAAAAGGCGTTGGCCGCCAAAAACCTCA
AGCCCGTTTATGTCGCCCGTTTTGCGCTGGGCGTGAAGGACCTCGGCGAAGAATTGAAGGCCGCCAAAGCGGCGGGCGCC
AACGTGGTGTTCAGCTATACCGTGGGACCGGAGAACGCCGTGATCGCGCATGGCCGCCAGGCCCTGAACTGGAAGGTGCC
GCAAGTGGGCGCATGGACCTTGTCATTTCCGTTTTTCATCGATGGCGGAAAGGATGCGGCCGATGGCGCACTGATGGCGC
AGACATTCATTGCCGAGCCCAGCAACGAGCGACGCGCTTCTTTTCTGACCAGTTACGCCCGCAAATTCAATGTCAGGAAA
ATTCCGGTGCCCATGGCGGCTGCGCAGGGCTATGACGCCGTCTACCTCCTGATCTACTCGCTGTTCGGCATACGCGACGG
CAACCTCAGCGGCCCTGCCATCAAGGCCGCCCTTGAAAGCAAGAGCCGCACCTATTACGGCGTGGTGGCTACCTATGACC
AGCCTTTCAGCAAGCAGGACAAAGACGCCACCACGCAGAACATGCTGGTGATGGGCATGGTGAAAAATGGCGCAGTCACG
TTTGCCTACCCCGAGGATGCCAAGCGCAACCTGTTTGTGCAGCGCAAGCAGTAG

Upstream 100 bases:

>100_bases
AGGCTGACGGCTCGCTGACGCCTGTCCCTTTTTTAGGGGAAAGTCTGGTGCGTCCCCGCGCCCGGCGCCACCTATCCTGC
ATGGCTACAGGAGACACAAC

Downstream 100 bases:

>100_bases
CCGCCCGCGCGCACCTGCAAGCCCGCGAGCCGCAGCCCGCGGCCGAGCCTATTTACAAACATCATCGCTTTCCGCACACT
CGGGCAAGATGCGTGCATGG

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MFINSRLAGFGVAGAVAAIVALGTALPAAAQGVSAAAAPPSPAANTLKIGVIGPFTGASSDFGVPMLNGIQQAVDEINAV
GGYLGRKLEIVRKDDKANPDVGLKMSQELVAEKVSVTLGFCNTGVAAKSLEVYQANKLPLIIPCATGSPLTAKYPAAESY
IFRTSASDSIQAPFVVEDILKRGWNRVAVFADTTGYGEAGLQDVEKALAAKNLKPVYVARFALGVKDLGEELKAAKAAGA
NVVFSYTVGPENAVIAHGRQALNWKVPQVGAWTLSFPFFIDGGKDAADGALMAQTFIAEPSNERRASFLTSYARKFNVRK
IPVPMAAAQGYDAVYLLIYSLFGIRDGNLSGPAIKAALESKSRTYYGVVATYDQPFSKQDKDATTQNMLVMGMVKNGAVT
FAYPEDAKRNLFVQRKQ

Sequences:

>Translated_417_residues
MFINSRLAGFGVAGAVAAIVALGTALPAAAQGVSAAAAPPSPAANTLKIGVIGPFTGASSDFGVPMLNGIQQAVDEINAV
GGYLGRKLEIVRKDDKANPDVGLKMSQELVAEKVSVTLGFCNTGVAAKSLEVYQANKLPLIIPCATGSPLTAKYPAAESY
IFRTSASDSIQAPFVVEDILKRGWNRVAVFADTTGYGEAGLQDVEKALAAKNLKPVYVARFALGVKDLGEELKAAKAAGA
NVVFSYTVGPENAVIAHGRQALNWKVPQVGAWTLSFPFFIDGGKDAADGALMAQTFIAEPSNERRASFLTSYARKFNVRK
IPVPMAAAQGYDAVYLLIYSLFGIRDGNLSGPAIKAALESKSRTYYGVVATYDQPFSKQDKDATTQNMLVMGMVKNGAVT
FAYPEDAKRNLFVQRKQ
>Mature_417_residues
MFINSRLAGFGVAGAVAAIVALGTALPAAAQGVSAAAAPPSPAANTLKIGVIGPFTGASSDFGVPMLNGIQQAVDEINAV
GGYLGRKLEIVRKDDKANPDVGLKMSQELVAEKVSVTLGFCNTGVAAKSLEVYQANKLPLIIPCATGSPLTAKYPAAESY
IFRTSASDSIQAPFVVEDILKRGWNRVAVFADTTGYGEAGLQDVEKALAAKNLKPVYVARFALGVKDLGEELKAAKAAGA
NVVFSYTVGPENAVIAHGRQALNWKVPQVGAWTLSFPFFIDGGKDAADGALMAQTFIAEPSNERRASFLTSYARKFNVRK
IPVPMAAAQGYDAVYLLIYSLFGIRDGNLSGPAIKAALESKSRTYYGVVATYDQPFSKQDKDATTQNMLVMGMVKNGAVT
FAYPEDAKRNLFVQRKQ

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=277, Percent_Identity=26.7148014440433, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI48994941, Length=228, Percent_Identity=27.6315789473684, Blast_Score=89, Evalue=8e-19,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 44078; Mature: 44078

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFINSRLAGFGVAGAVAAIVALGTALPAAAQGVSAAAAPPSPAANTLKIGVIGPFTGASS
CCCCCCCCCCCHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCC
DFGVPMLNGIQQAVDEINAVGGYLGRKLEIVRKDDKANPDVGLKMSQELVAEKVSVTLGF
CCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCHHHHHHHHHHEEEEEE
CNTGVAAKSLEVYQANKLPLIIPCATGSPLTAKYPAAESYIFRTSASDSIQAPFVVEDIL
CCCCCCCCEEEEEECCCCCEEEEECCCCCCEECCCCCCCEEEEECCCCCCCCCHHHHHHH
KRGWNRVAVFADTTGYGEAGLQDVEKALAAKNLKPVYVARFALGVKDLGEELKAAKAAGA
HCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHCCCC
NVVFSYTVGPENAVIAHGRQALNWKVPQVGAWTLSFPFFIDGGKDAADGALMAQTFIAEP
EEEEEEEECCCCEEEECCCHHCEEECCCCCEEEEECCEEEECCCCCCCCHHHHHHHHCCC
SNERRASFLTSYARKFNVRKIPVPMAAAQGYDAVYLLIYSLFGIRDGNLSGPAIKAALES
CCHHHHHHHHHHHHHCCCEECCCCHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHC
KSRTYYGVVATYDQPFSKQDKDATTQNMLVMGMVKNGAVTFAYPEDAKRNLFVQRKQ
CCCEEEEEEEECCCCCCCCCCCCCHHCEEEEEEECCCEEEEECCCCCCCCEEEEECC
>Mature Secondary Structure
MFINSRLAGFGVAGAVAAIVALGTALPAAAQGVSAAAAPPSPAANTLKIGVIGPFTGASS
CCCCCCCCCCCHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCC
DFGVPMLNGIQQAVDEINAVGGYLGRKLEIVRKDDKANPDVGLKMSQELVAEKVSVTLGF
CCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCHHHHHHHHHHEEEEEE
CNTGVAAKSLEVYQANKLPLIIPCATGSPLTAKYPAAESYIFRTSASDSIQAPFVVEDIL
CCCCCCCCEEEEEECCCCCEEEEECCCCCCEECCCCCCCEEEEECCCCCCCCCHHHHHHH
KRGWNRVAVFADTTGYGEAGLQDVEKALAAKNLKPVYVARFALGVKDLGEELKAAKAAGA
HCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHCCCC
NVVFSYTVGPENAVIAHGRQALNWKVPQVGAWTLSFPFFIDGGKDAADGALMAQTFIAEP
EEEEEEEECCCCEEEECCCHHCEEECCCCCEEEEECCEEEECCCCCCCCHHHHHHHHCCC
SNERRASFLTSYARKFNVRKIPVPMAAAQGYDAVYLLIYSLFGIRDGNLSGPAIKAALES
CCHHHHHHHHHHHHHCCCEECCCCHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHC
KSRTYYGVVATYDQPFSKQDKDATTQNMLVMGMVKNGAVTFAYPEDAKRNLFVQRKQ
CCCEEEEEEEECCCCCCCCCCCCCHHCEEEEEEECCCEEEEECCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]