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The map label for this gene is gsiB [H]

Identifier: 91786050

GI number: 91786050

Start: 145010

End: 146572

Strand: Reverse

Name: gsiB [H]

Synonym: Bpro_0139

Alternate gene names: 91786050

Gene position: 146572-145010 (Counterclockwise)

Preceding gene: 91786051

Following gene: 91786049

Centisome position: 2.82

GC content: 61.55

Gene sequence:

>1563_bases
ATGACAGAACGTATGACACCGCTTCGACCCAAGGCCCTGGCTGCCAGCCTGCTGGCGCTGCTGGCCCTGGCCTCCACTGG
CTCGGCCCTTGCCGCCAAGGACGTGGTACTGGCCATCGGCGGCCAGCCCGAAACGCTGGACCCCTACAACACCAACACCA
CGCTGACCACGGCAGTCACCAAGTCCTTCTACCAGGGCCTGTTCGAGTTCGACAAGGACCTCAAAATCCAGAACGTGCTG
GCCGAAAGCTACACCGCCTCCAAAGACGGCCTTGTCTACACCCTCAAGCTGCGCCAGGGCGTCAAGTTCCATGACGGCAC
CGACTTCAATGCCGAAGCCGTCAAGGTCACGCTGGACCGGGTGCTCAACCCCGACAACAAACTGGCCCGCTTCAACCAGT
TCAACCGCGTGGACAAGGTCGAGGCGATTGCACCGTACAGCGTGCGCATCACGCTCAAGGAGCCTTTTGGCCCCTTCATC
AACTCGCTGGCGCACGCCTCGGCCGCGATGATCTCCCCGACGGCACTCAAAAAGTACGGCAACAAGGACATCGCCTTCAA
CCCGGTGGGCACCGGCCCCTTCACTTTTGTGGAGTGGAAGCAGACCGATTTCGTCAAGGTCAGGAAATTCGACGGCTACT
GGAAAAAGGGCTACCCCAAGGTCGACAGCGTGACCTGGAAACCCGTGCTGGAGAACAACACACGCGCCGCCATGCTGCAG
ACCGGCGAAACCGACTTTGCCTTCCCGCTGCCCTACGAGCAGGCCACCGAACTGGAGAAAAACGCCAAGCTCAAGCTGGT
GAGCGGCCCGTCCATCATCACGCGCTACCTCAGCTTCAACATGCAGCAAAAGCCTTTCGACAACCTCAAGGTGCGCGAAG
CCATCAACTACGCCATCAACAAGGAAGCGCTCGCCAAAGTGGCCTTCGGTGGCTATGCCGTCCCGGCCGAGGGCATCGTG
CCGCAAGGGGTGAAGTACGCCCACAAGATGGCGCCCTGGCCTTATGACCCGAAGAAGGCGCGCGAGCTGCTCAAGGAAGC
CGGTTACGCCAACGGCTTCGAGTCGGTGCTCTGGAGCGCCTACACCACCACCACCGCGCAGAAAACCATCCAGTTCGTGC
AGCAGCAGCTGGCCCAGGTGGGCATCAAGATCTCGGTGCAGTCACTCGAACCCGGCCAGCGTACCGAGTGGGTGCAAACC
GCGCCGGACCCCAAAACCGCGAAAGTGCGCATGTACTACGCCGGCTGGTCTTCTTCGACCGGTGAAGCCGACTGGGCGCT
GCGCCCGCTGCTGGCCACCGAAGCCTGGCCGCCCAAGCTGAACAACACCGCCTACTACAGCAACGAGAAGGTGGACGGCG
CTATCGCCAAGGCCCTGAAGTCGGTCGATGACAAGGAAAAAGCCGACCTGTATCGCCAGGCACAGGAGCAGATCATGAAA
GACGCGCCCTGGGCGCCGCTGGTGACGGAGAAAAACCTGTACGCCACAAGCAAGCGCCTGTCGGGCGTCTACGTGATGCC
TGACGGCAACATCAATGCGGACGAGATCGCGGTCGCCGAGTAA

Upstream 100 bases:

>100_bases
CATTTTGTGGCGCAGCACCGCATTGCCGGTGCGTATTGACTCGCCCATCGATCCGCTTTCCCTCCATCATTTCCCTCAAC
CTGAACCAGGAGTTTTCCCC

Downstream 100 bases:

>100_bases
GCATTTCCTTTGCGCCTTCTTTGCACCGATCCCGCGCGCGCCAGCATCCGCTGGCGCGCGCCCTGACCAGTCTCTACCAT
GCTGAACTATTTTCTGAAAC

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 520; Mature: 519

Protein sequence:

>520_residues
MTERMTPLRPKALAASLLALLALASTGSALAAKDVVLAIGGQPETLDPYNTNTTLTTAVTKSFYQGLFEFDKDLKIQNVL
AESYTASKDGLVYTLKLRQGVKFHDGTDFNAEAVKVTLDRVLNPDNKLARFNQFNRVDKVEAIAPYSVRITLKEPFGPFI
NSLAHASAAMISPTALKKYGNKDIAFNPVGTGPFTFVEWKQTDFVKVRKFDGYWKKGYPKVDSVTWKPVLENNTRAAMLQ
TGETDFAFPLPYEQATELEKNAKLKLVSGPSIITRYLSFNMQQKPFDNLKVREAINYAINKEALAKVAFGGYAVPAEGIV
PQGVKYAHKMAPWPYDPKKARELLKEAGYANGFESVLWSAYTTTTAQKTIQFVQQQLAQVGIKISVQSLEPGQRTEWVQT
APDPKTAKVRMYYAGWSSSTGEADWALRPLLATEAWPPKLNNTAYYSNEKVDGAIAKALKSVDDKEKADLYRQAQEQIMK
DAPWAPLVTEKNLYATSKRLSGVYVMPDGNINADEIAVAE

Sequences:

>Translated_520_residues
MTERMTPLRPKALAASLLALLALASTGSALAAKDVVLAIGGQPETLDPYNTNTTLTTAVTKSFYQGLFEFDKDLKIQNVL
AESYTASKDGLVYTLKLRQGVKFHDGTDFNAEAVKVTLDRVLNPDNKLARFNQFNRVDKVEAIAPYSVRITLKEPFGPFI
NSLAHASAAMISPTALKKYGNKDIAFNPVGTGPFTFVEWKQTDFVKVRKFDGYWKKGYPKVDSVTWKPVLENNTRAAMLQ
TGETDFAFPLPYEQATELEKNAKLKLVSGPSIITRYLSFNMQQKPFDNLKVREAINYAINKEALAKVAFGGYAVPAEGIV
PQGVKYAHKMAPWPYDPKKARELLKEAGYANGFESVLWSAYTTTTAQKTIQFVQQQLAQVGIKISVQSLEPGQRTEWVQT
APDPKTAKVRMYYAGWSSSTGEADWALRPLLATEAWPPKLNNTAYYSNEKVDGAIAKALKSVDDKEKADLYRQAQEQIMK
DAPWAPLVTEKNLYATSKRLSGVYVMPDGNINADEIAVAE
>Mature_519_residues
TERMTPLRPKALAASLLALLALASTGSALAAKDVVLAIGGQPETLDPYNTNTTLTTAVTKSFYQGLFEFDKDLKIQNVLA
ESYTASKDGLVYTLKLRQGVKFHDGTDFNAEAVKVTLDRVLNPDNKLARFNQFNRVDKVEAIAPYSVRITLKEPFGPFIN
SLAHASAAMISPTALKKYGNKDIAFNPVGTGPFTFVEWKQTDFVKVRKFDGYWKKGYPKVDSVTWKPVLENNTRAAMLQT
GETDFAFPLPYEQATELEKNAKLKLVSGPSIITRYLSFNMQQKPFDNLKVREAINYAINKEALAKVAFGGYAVPAEGIVP
QGVKYAHKMAPWPYDPKKARELLKEAGYANGFESVLWSAYTTTTAQKTIQFVQQQLAQVGIKISVQSLEPGQRTEWVQTA
PDPKTAKVRMYYAGWSSSTGEADWALRPLLATEAWPPKLNNTAYYSNEKVDGAIAKALKSVDDKEKADLYRQAQEQIMKD
APWAPLVTEKNLYATSKRLSGVYVMPDGNINADEIAVAE

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=501, Percent_Identity=59.8802395209581, Blast_Score=619, Evalue=1e-178,
Organism=Escherichia coli, GI1789966, Length=535, Percent_Identity=28.5981308411215, Blast_Score=202, Evalue=5e-53,
Organism=Escherichia coli, GI1787551, Length=541, Percent_Identity=27.1719038817006, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1787762, Length=523, Percent_Identity=25.4302103250478, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI1787495, Length=502, Percent_Identity=24.5019920318725, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1789887, Length=511, Percent_Identity=26.027397260274, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1789397, Length=471, Percent_Identity=21.2314225053079, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87081878, Length=495, Percent_Identity=21.8181818181818, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI87082063, Length=273, Percent_Identity=25.6410256410256, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 57660; Mature: 57529

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTERMTPLRPKALAASLLALLALASTGSALAAKDVVLAIGGQPETLDPYNTNTTLTTAVT
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEECCEEEEECCCCCCCCCCCCCCEEHHHHH
KSFYQGLFEFDKDLKIQNVLAESYTASKDGLVYTLKLRQGVKFHDGTDFNAEAVKVTLDR
HHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCEECCCCCCCCHHHEEEHHH
VLNPDNKLARFNQFNRVDKVEAIAPYSVRITLKEPFGPFINSLAHASAAMISPTALKKYG
HCCCCHHHHHHHHHCCHHHHHEECCEEEEEEEECCCCHHHHHHHHHHHHHCCHHHHHHCC
NKDIAFNPVGTGPFTFVEWKQTDFVKVRKFDGYWKKGYPKVDSVTWKPVLENNTRAAMLQ
CCCEEECCCCCCCEEEEEECCCCEEEEEECCCHHHCCCCCCCCEEECHHHCCCCCEEEEE
TGETDFAFPLPYEQATELEKNAKLKLVSGPSIITRYLSFNMQQKPFDNLKVREAINYAIN
CCCCCCCCCCCCHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHC
KEALAKVAFGGYAVPAEGIVPQGVKYAHKMAPWPYDPKKARELLKEAGYANGFESVLWSA
HHHHHHHHCCCEECCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHH
YTTTTAQKTIQFVQQQLAQVGIKISVQSLEPGQRTEWVQTAPDPKTAKVRMYYAGWSSST
HHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCC
GEADWALRPLLATEAWPPKLNNTAYYSNEKVDGAIAKALKSVDDKEKADLYRQAQEQIMK
CCCCHHHHHHHHCCCCCCCCCCCEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
DAPWAPLVTEKNLYATSKRLSGVYVMPDGNINADEIAVAE
CCCCCCEEECCCHHHHHHHCCEEEEEECCCCCCCCEEECC
>Mature Secondary Structure 
TERMTPLRPKALAASLLALLALASTGSALAAKDVVLAIGGQPETLDPYNTNTTLTTAVT
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCEECCEEEEECCCCCCCCCCCCCCEEHHHHH
KSFYQGLFEFDKDLKIQNVLAESYTASKDGLVYTLKLRQGVKFHDGTDFNAEAVKVTLDR
HHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCEECCCCCCCCHHHEEEHHH
VLNPDNKLARFNQFNRVDKVEAIAPYSVRITLKEPFGPFINSLAHASAAMISPTALKKYG
HCCCCHHHHHHHHHCCHHHHHEECCEEEEEEEECCCCHHHHHHHHHHHHHCCHHHHHHCC
NKDIAFNPVGTGPFTFVEWKQTDFVKVRKFDGYWKKGYPKVDSVTWKPVLENNTRAAMLQ
CCCEEECCCCCCCEEEEEECCCCEEEEEECCCHHHCCCCCCCCEEECHHHCCCCCEEEEE
TGETDFAFPLPYEQATELEKNAKLKLVSGPSIITRYLSFNMQQKPFDNLKVREAINYAIN
CCCCCCCCCCCCHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHC
KEALAKVAFGGYAVPAEGIVPQGVKYAHKMAPWPYDPKKARELLKEAGYANGFESVLWSA
HHHHHHHHCCCEECCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHH
YTTTTAQKTIQFVQQQLAQVGIKISVQSLEPGQRTEWVQTAPDPKTAKVRMYYAGWSSST
HHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCC
GEADWALRPLLATEAWPPKLNNTAYYSNEKVDGAIAKALKSVDDKEKADLYRQAQEQIMK
CCCCHHHHHHHHCCCCCCCCCCCEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
DAPWAPLVTEKNLYATSKRLSGVYVMPDGNINADEIAVAE
CCCCCCEEECCCHHHHHHHCCEEEEEECCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA