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The map label for this gene is gyrB [H]

Identifier: 91785916

GI number: 91785916

Start: 2944

End: 5568

Strand: Direct

Name: gyrB [H]

Synonym: Bpro_0003

Alternate gene names: 91785916

Gene position: 2944-5568 (Clockwise)

Preceding gene: 91785915

Following gene: 91785917

Centisome position: 0.06

GC content: 62.59

Gene sequence:

>2625_bases
ATGACTGAAGAAAACAATGTGAACGGGGCCGATCAGGTGCACGTAACTGACGGTACTGCGGGCGAGGAAAGCTCCAATTT
CCAGCCCAAAATCGACACCAACCAGGCCGGCGCTTCAGAAGCCTACGGCGAAGGCTCGATCCAGATCCTGGAAGGCCTGG
AAGCCGTGCGCAAGCGCCCCGGCATGTACATTGGCGACACTTCAGACGGCACCGGCTTGCACCACCTGGTGTTCGAGGTG
GTCGACAACTCGATTGACGAAGCACTGGCCGGCCACTGCGACGACATCGTCGTCACCATCCACACCGACAACTCCATCAG
CGTGACCGACAACGGCCGCGGCATTCCGACCGGCATCAAGATGGACGATAAGCATGAGCCCAAACGCTCGGCCGCCGAGA
TTGCCCTGACCGAGCTGCATGCCGGCGGCAAGTTCAACCAGAACAGCTACAAGGTCTCGGGTGGCCTGCACGGCGTGGGC
GTGAGTTGCGTGAATGCGCTGTCAAAAATGCTGCGCCTCACCATTCGCCGCGACGGCAAGGTGCATGTCATGGAGTTCTC
GCGCGGCTTTGTTCAAAACCGCATCATCGAGCAGATCAACGGCGTGGATGTCTCGCCGATGAAGGTGATGGACCACACCG
ACAAGCGCGGCACCGAAGTCCACTTTTTGCCCGACACTGAAATCTTCAAAGAGAACAACGATTTCCATTACGAGATCTTG
AGCAAGCGCCTGCGCGAGCTGAGTTTTTTGAACAACGGCGTGCGCATTCGCCTGAAAGACGAGCGCAGCGGCAAGGAAGA
CGACTTTGCCGGCGCCGGCGGCGTGAAAGGCTTTGTTGAATTCATCAACAAGGGCAAGACGGTGCTGCACCCCAACGTGT
TTGCCGCCATGGGCGACCGCCAGAGCGACCAGGGCACCAACATCGGCGTGGAAGTCGCCATGCAGTGGAACAGCGGCTAC
AGCGAACAGGTGCTGTGTTTCACCAACAACATTCCGCAGCGCGACGGCGGCACCCACCTGACCGGCCTGCGCGCCGCGAT
GACGCGCGTCATCAACAAATACATCGACGACAGCGAGCTGGCCAAAAAAGCCAAGGTGGAAGTCACGGGCGACGACATGC
GCGAAGGCCTGTGCTGCGTGCTGAGCGTGAAGGTGCCCGAGCCCAAGTTCTCCAGCCAGACCAAGGACAAGCTGGTGAGC
AGCGAGGTGCGCGGCCCGGTCGAAGACATTGTGAGCCGCCTGTTGCACGACTACCTGCAGGAGCGCCCGAACGACGCCAA
GATCATCGTCGGCAAGATCATTGAGGCCGCCCGCGCTCGCGAAGCCGCGCGCAAGGCGCGCGACATGACGCGCCGCAAGG
GCGTGCTCGACGGCATGGGCCTGCCCGGCAAGCTGGCCGACTGCCAGGAAAAAGACCCCGCCATGTGCGAGATCTACCTG
GTCGAGGGTGACTCCGCCGGTGGCTCCGCCAAGCAGGGCCGCGACCGTAAATTCCAGGCCATCCTGCCGCTGCGCGGCAA
GATCCTGAACGTTGAAAAAGCCCGCTACGAAAAGCTGCTGACCAGCAACGAGATTTTGACGCTGATCACCGCGCTGGGCA
CCGGCATCGGCAAGGCCGGCGGCATTGGCGGCACGCCCGGCACTGACGACTTCAACGTTGCCAAGCTGCGCTACCACCGC
ATCATCATCATGACCGACGCCGACGTGGACGGCGCGCACATCCGCACGCTGTTGCTGACCTTCTTTTACCGCCAGATGCC
CGAGCTGGTCGAGCGCGGCCACATCTACATCGCGCAGCCACCGCTGTACAAGGTCAAGGCCGGCAAGGAAGAGCAGTACC
TCAAGGACACCGCCGCACTGGACGGCTTTTTGCTGCGCATTGCGCTCAAGGATGCGAGCGTGCAGACCGGCGCCGACGCA
AGCACCGTGCTTACCGGCGACACGCTGGCCGAGCTGGCCCGCAAGCACCAGCTGGCCGAGTCAGTGATTGCCCGCCTGCG
CGGCTTCATGGACGCGGAAGCCCTGCGCGCCATTGCCGACGGCGTGAGCCTGAACCTGGACACCGTGGCCGATGCGGAAG
CCTCCGCCGTGACGCTGCAGGTCAAGCTGCGCGAACTGAACACCACGGGCGCGCCGGCCGACGTGGCCGGCGAGTTTGAC
ACCCGCACCGACAAACCCATTCTGCGCATCAGCCGCCGCCACCACGGCAACGTGAAAAGCAGCGTGATCACGCAGGACTT
TGTGCACGGCGCCGACTACGCCACCCTGGCCGATGCCGCCGCCACCTTCCGCGGCCTCATCAGCGAAGGCGCCAAGGTCA
TGCGCGGCGAAGGCGAGCGCCAGAAGGAAGAAAAAGTCAGCGACTTCCGCCAGGCCATGGCCTGGCTGATCGGCCAGGCC
GAAAACGCCACCGCCCGTCAGCGCTACAAAGGCCTGGGCGAGATGAACCCGGCCCAGCTATGGGAGACGACGATGGACCC
ACAGGTGCGCCGTTTGCTACGCGTGCAGATTGACGACGCCATTGAAGCCGACCATGTGTTCACCATGCTGATGGGTGATG
AGGTCGAGCCGCGTCGGGAGTTTATTGAGCAGAATGCGTTGCGGGCGGCGAATATTGATGTCTAA

Upstream 100 bases:

>100_bases
CTAAAAAGCGCGCCCAAATTCAAACAGACCCGCTTGCTAGAGCGGGTTTGGTCATTCAAGAGATTGAAGAAATCGAAAAG
ATTTGGAAGTAAAGCTGGTT

Downstream 100 bases:

>100_bases
CGCCTGAACAAGTTGTGACGAGTCGGGCCTACTACTCAGAACTCATTGAAGAGTTCGTCGGTGAGTCGTCAGACCAAATA
TTGGGGGTTCTTTCACGGCG

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 874; Mature: 873

Protein sequence:

>874_residues
MTEENNVNGADQVHVTDGTAGEESSNFQPKIDTNQAGASEAYGEGSIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEV
VDNSIDEALAGHCDDIVVTIHTDNSISVTDNGRGIPTGIKMDDKHEPKRSAAEIALTELHAGGKFNQNSYKVSGGLHGVG
VSCVNALSKMLRLTIRRDGKVHVMEFSRGFVQNRIIEQINGVDVSPMKVMDHTDKRGTEVHFLPDTEIFKENNDFHYEIL
SKRLRELSFLNNGVRIRLKDERSGKEDDFAGAGGVKGFVEFINKGKTVLHPNVFAAMGDRQSDQGTNIGVEVAMQWNSGY
SEQVLCFTNNIPQRDGGTHLTGLRAAMTRVINKYIDDSELAKKAKVEVTGDDMREGLCCVLSVKVPEPKFSSQTKDKLVS
SEVRGPVEDIVSRLLHDYLQERPNDAKIIVGKIIEAARAREAARKARDMTRRKGVLDGMGLPGKLADCQEKDPAMCEIYL
VEGDSAGGSAKQGRDRKFQAILPLRGKILNVEKARYEKLLTSNEILTLITALGTGIGKAGGIGGTPGTDDFNVAKLRYHR
IIIMTDADVDGAHIRTLLLTFFYRQMPELVERGHIYIAQPPLYKVKAGKEEQYLKDTAALDGFLLRIALKDASVQTGADA
STVLTGDTLAELARKHQLAESVIARLRGFMDAEALRAIADGVSLNLDTVADAEASAVTLQVKLRELNTTGAPADVAGEFD
TRTDKPILRISRRHHGNVKSSVITQDFVHGADYATLADAAATFRGLISEGAKVMRGEGERQKEEKVSDFRQAMAWLIGQA
ENATARQRYKGLGEMNPAQLWETTMDPQVRRLLRVQIDDAIEADHVFTMLMGDEVEPRREFIEQNALRAANIDV

Sequences:

>Translated_874_residues
MTEENNVNGADQVHVTDGTAGEESSNFQPKIDTNQAGASEAYGEGSIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEV
VDNSIDEALAGHCDDIVVTIHTDNSISVTDNGRGIPTGIKMDDKHEPKRSAAEIALTELHAGGKFNQNSYKVSGGLHGVG
VSCVNALSKMLRLTIRRDGKVHVMEFSRGFVQNRIIEQINGVDVSPMKVMDHTDKRGTEVHFLPDTEIFKENNDFHYEIL
SKRLRELSFLNNGVRIRLKDERSGKEDDFAGAGGVKGFVEFINKGKTVLHPNVFAAMGDRQSDQGTNIGVEVAMQWNSGY
SEQVLCFTNNIPQRDGGTHLTGLRAAMTRVINKYIDDSELAKKAKVEVTGDDMREGLCCVLSVKVPEPKFSSQTKDKLVS
SEVRGPVEDIVSRLLHDYLQERPNDAKIIVGKIIEAARAREAARKARDMTRRKGVLDGMGLPGKLADCQEKDPAMCEIYL
VEGDSAGGSAKQGRDRKFQAILPLRGKILNVEKARYEKLLTSNEILTLITALGTGIGKAGGIGGTPGTDDFNVAKLRYHR
IIIMTDADVDGAHIRTLLLTFFYRQMPELVERGHIYIAQPPLYKVKAGKEEQYLKDTAALDGFLLRIALKDASVQTGADA
STVLTGDTLAELARKHQLAESVIARLRGFMDAEALRAIADGVSLNLDTVADAEASAVTLQVKLRELNTTGAPADVAGEFD
TRTDKPILRISRRHHGNVKSSVITQDFVHGADYATLADAAATFRGLISEGAKVMRGEGERQKEEKVSDFRQAMAWLIGQA
ENATARQRYKGLGEMNPAQLWETTMDPQVRRLLRVQIDDAIEADHVFTMLMGDEVEPRREFIEQNALRAANIDV
>Mature_873_residues
TEENNVNGADQVHVTDGTAGEESSNFQPKIDTNQAGASEAYGEGSIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVV
DNSIDEALAGHCDDIVVTIHTDNSISVTDNGRGIPTGIKMDDKHEPKRSAAEIALTELHAGGKFNQNSYKVSGGLHGVGV
SCVNALSKMLRLTIRRDGKVHVMEFSRGFVQNRIIEQINGVDVSPMKVMDHTDKRGTEVHFLPDTEIFKENNDFHYEILS
KRLRELSFLNNGVRIRLKDERSGKEDDFAGAGGVKGFVEFINKGKTVLHPNVFAAMGDRQSDQGTNIGVEVAMQWNSGYS
EQVLCFTNNIPQRDGGTHLTGLRAAMTRVINKYIDDSELAKKAKVEVTGDDMREGLCCVLSVKVPEPKFSSQTKDKLVSS
EVRGPVEDIVSRLLHDYLQERPNDAKIIVGKIIEAARAREAARKARDMTRRKGVLDGMGLPGKLADCQEKDPAMCEIYLV
EGDSAGGSAKQGRDRKFQAILPLRGKILNVEKARYEKLLTSNEILTLITALGTGIGKAGGIGGTPGTDDFNVAKLRYHRI
IIMTDADVDGAHIRTLLLTFFYRQMPELVERGHIYIAQPPLYKVKAGKEEQYLKDTAALDGFLLRIALKDASVQTGADAS
TVLTGDTLAELARKHQLAESVIARLRGFMDAEALRAIADGVSLNLDTVADAEASAVTLQVKLRELNTTGAPADVAGEFDT
RTDKPILRISRRHHGNVKSSVITQDFVHGADYATLADAAATFRGLISEGAKVMRGEGERQKEEKVSDFRQAMAWLIGQAE
NATARQRYKGLGEMNPAQLWETTMDPQVRRLLRVQIDDAIEADHVFTMLMGDEVEPRREFIEQNALRAANIDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=595, Percent_Identity=24.7058823529412, Blast_Score=116, Evalue=8e-26,
Organism=Homo sapiens, GI19913406, Length=642, Percent_Identity=23.208722741433, Blast_Score=111, Evalue=3e-24,
Organism=Escherichia coli, GI48994957, Length=842, Percent_Identity=57.3634204275535, Blast_Score=925, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=580, Percent_Identity=37.9310344827586, Blast_Score=359, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17535065, Length=631, Percent_Identity=25.0396196513471, Blast_Score=139, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI212645845, Length=539, Percent_Identity=25.2319109461967, Blast_Score=109, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI212645657, Length=160, Percent_Identity=34.375, Blast_Score=76, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=636, Percent_Identity=24.3710691823899, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI17136538, Length=562, Percent_Identity=24.5551601423488, Blast_Score=117, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 95884; Mature: 95753

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEENNVNGADQVHVTDGTAGEESSNFQPKIDTNQAGASEAYGEGSIQILEGLEAVRKRP
CCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
GMYIGDTSDGTGLHHLVFEVVDNSIDEALAGHCDDIVVTIHTDNSISVTDNGRGIPTGIK
CEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEECCCCCCCCCCCC
MDDKHEPKRSAAEIALTELHAGGKFNQNSYKVSGGLHGVGVSCVNALSKMLRLTIRRDGK
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCC
VHVMEFSRGFVQNRIIEQINGVDVSPMKVMDHTDKRGTEVHFLPDTEIFKENNDFHYEIL
EEEEEHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCEEEECCCHHHHCCCCCHHHHHH
SKRLRELSFLNNGVRIRLKDERSGKEDDFAGAGGVKGFVEFINKGKTVLHPNVFAAMGDR
HHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCEEECCCHHHHCCCC
QSDQGTNIGVEVAMQWNSGYSEQVLCFTNNIPQRDGGTHLTGLRAAMTRVINKYIDDSEL
CCCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHH
AKKAKVEVTGDDMREGLCCVLSVKVPEPKFSSQTKDKLVSSEVRGPVEDIVSRLLHDYLQ
HHHCEEEECCCHHHCCEEEEEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ERPNDAKIIVGKIIEAARAREAARKARDMTRRKGVLDGMGLPGKLADCQEKDPAMCEIYL
CCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEE
VEGDSAGGSAKQGRDRKFQAILPLRGKILNVEKARYEKLLTSNEILTLITALGTGIGKAG
EECCCCCCCCCCCCCCCEEEECCCCCEEECHHHHHHHHHHCCCHHHHHHHHHHCCCCCCC
GIGGTPGTDDFNVAKLRYHRIIIMTDADVDGAHIRTLLLTFFYRQMPELVERGHIYIAQP
CCCCCCCCCCCCEEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
PLYKVKAGKEEQYLKDTAALDGFLLRIALKDASVQTGADASTVLTGDTLAELARKHQLAE
CCEEECCCCHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCEEECCHHHHHHHHHHHHHH
SVIARLRGFMDAEALRAIADGVSLNLDTVADAEASAVTLQVKLRELNTTGAPADVAGEFD
HHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCC
TRTDKPILRISRRHHGNVKSSVITQDFVHGADYATLADAAATFRGLISEGAKVMRGEGER
CCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCHHHHCCCCCH
QKEEKVSDFRQAMAWLIGQAENATARQRYKGLGEMNPAQLWETTMDPQVRRLLRVQIDDA
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHH
IEADHVFTMLMGDEVEPRREFIEQNALRAANIDV
CCHHHEEEEECCCCCCHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TEENNVNGADQVHVTDGTAGEESSNFQPKIDTNQAGASEAYGEGSIQILEGLEAVRKRP
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
GMYIGDTSDGTGLHHLVFEVVDNSIDEALAGHCDDIVVTIHTDNSISVTDNGRGIPTGIK
CEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEECCCCCCCCCCCC
MDDKHEPKRSAAEIALTELHAGGKFNQNSYKVSGGLHGVGVSCVNALSKMLRLTIRRDGK
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCC
VHVMEFSRGFVQNRIIEQINGVDVSPMKVMDHTDKRGTEVHFLPDTEIFKENNDFHYEIL
EEEEEHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCEEEECCCHHHHCCCCCHHHHHH
SKRLRELSFLNNGVRIRLKDERSGKEDDFAGAGGVKGFVEFINKGKTVLHPNVFAAMGDR
HHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCEEECCCHHHHCCCC
QSDQGTNIGVEVAMQWNSGYSEQVLCFTNNIPQRDGGTHLTGLRAAMTRVINKYIDDSEL
CCCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHH
AKKAKVEVTGDDMREGLCCVLSVKVPEPKFSSQTKDKLVSSEVRGPVEDIVSRLLHDYLQ
HHHCEEEECCCHHHCCEEEEEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ERPNDAKIIVGKIIEAARAREAARKARDMTRRKGVLDGMGLPGKLADCQEKDPAMCEIYL
CCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEE
VEGDSAGGSAKQGRDRKFQAILPLRGKILNVEKARYEKLLTSNEILTLITALGTGIGKAG
EECCCCCCCCCCCCCCCEEEECCCCCEEECHHHHHHHHHHCCCHHHHHHHHHHCCCCCCC
GIGGTPGTDDFNVAKLRYHRIIIMTDADVDGAHIRTLLLTFFYRQMPELVERGHIYIAQP
CCCCCCCCCCCCEEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
PLYKVKAGKEEQYLKDTAALDGFLLRIALKDASVQTGADASTVLTGDTLAELARKHQLAE
CCEEECCCCHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCEEECCHHHHHHHHHHHHHH
SVIARLRGFMDAEALRAIADGVSLNLDTVADAEASAVTLQVKLRELNTTGAPADVAGEFD
HHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCC
TRTDKPILRISRRHHGNVKSSVITQDFVHGADYATLADAAATFRGLISEGAKVMRGEGER
CCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCHHHHCCCCCH
QKEEKVSDFRQAMAWLIGQAENATARQRYKGLGEMNPAQLWETTMDPQVRRLLRVQIDDA
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHH
IEADHVFTMLMGDEVEPRREFIEQNALRAANIDV
CCHHHEEEEECCCCCCHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]