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The map label for this gene is lasT [H]

Identifier: 91214121

GI number: 91214121

Start: 5065010

End: 5065717

Strand: Direct

Name: lasT [H]

Synonym: UTI89_C5176

Alternate gene names: 91214121

Gene position: 5065010-5065717 (Clockwise)

Preceding gene: 91214120

Following gene: NA

Centisome position: 99.99

GC content: 48.16

Gene sequence:

>708_bases
TTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTTGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGC
GCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTTGATAGTCAGGCACACTTGGAGCCAGTCGCCCGTTGGG
TCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCAAGACACTAGCTGAATCGTTACACGATGTCGATTTC
ACTGTTGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTGCCGCTGTTAGAGGA
AAAATCTTCATGGATGAGCCATGCCGCTCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGG
CTGACGTTCTTACTGGTGTGCCGATGGTGGCGGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTAT
CAATTAGCAACATTAATACAACAACCGGCGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACG
AGCCATGACATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAACAACGCCTGGGGCTTT
TAGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAA

Upstream 100 bases:

>100_bases
TTTTTAAGCTAAGCAAAGGGCTTTTCCTGCGACTTACGTTAAGAATTTGTAAATTCGCAACGCGTAATAAGTTGACAATG
ATCACCCTGTTCGCGGTTAT

Downstream 100 bases:

>100_bases
AAAACGCCTTAGTAAGTGATTTTCAGCTTTTCATTCTGACTGCAACGGGCAATATGTCTCTGTGTGGATTAAAAAAAGAG
TGTCTGATAGCAGCTTCTGA

Product: putative RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MVKKSGNMRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPVARWVAHGSGDIIDNIKVFKTLAESLHDVDF
TVATTARSRAKYHYYATPVELVPLLEEKSSWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCY
QLATLIQQPAKSDTTADQHQLQALRERAMTLLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRLLHDIEKNITK

Sequences:

>Translated_235_residues
MVKKSGNMRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPVARWVAHGSGDIIDNIKVFKTLAESLHDVDF
TVATTARSRAKYHYYATPVELVPLLEEKSSWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCY
QLATLIQQPAKSDTTADQHQLQALRERAMTLLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRLLHDIEKNITK
>Mature_235_residues
MVKKSGNMRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPVARWVAHGSGDIIDNIKVFKTLAESLHDVDF
TVATTARSRAKYHYYATPVELVPLLEEKSSWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCY
QLATLIQQPAKSDTTADQHQLQALRERAMTLLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRLLHDIEKNITK

Specific function: Unknown

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790865, Length=228, Percent_Identity=97.8070175438597, Blast_Score=458, Evalue=1e-130,
Organism=Escherichia coli, GI1788881, Length=153, Percent_Identity=37.9084967320261, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26077; Mature: 26077

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKKSGNMRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPVARWVAHGSGD
CCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHCCHHHHEEECCHHHHHHHHHHHHCCCCC
IIDNIKVFKTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKSSWMSHAALVFG
HHHHHHHHHHHHHHHHHCCHHEEHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHEEE
REDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQQPAKSDTTADQHQ
CCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
LQALRERAMTLLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRLLHDIEKNITK
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVKKSGNMRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPVARWVAHGSGD
CCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHCCHHHHEEECCHHHHHHHHHHHHCCCCC
IIDNIKVFKTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKSSWMSHAALVFG
HHHHHHHHHHHHHHHHHCCHHEEHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHEEE
REDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQQPAKSDTTADQHQ
CCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
LQALRERAMTLLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRLLHDIEKNITK
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 8022271 [H]