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The map label for this gene is ybcS2 [H]

Identifier: 91214057

GI number: 91214057

Start: 5005389

End: 5005886

Strand: Direct

Name: ybcS2 [H]

Synonym: UTI89_C5112

Alternate gene names: 91214057

Gene position: 5005389-5005886 (Clockwise)

Preceding gene: 91214056

Following gene: 91214058

Centisome position: 98.81

GC content: 49.2

Gene sequence:

>498_bases
ATGCCTCCATCATTACGAAAAGCAGTTGCTGCTGCTATTGGTGGCGGAGCAATTGCTATAGCATCAGTGTTAATCACTGG
CCCAAGTGGTGACGATGGCCTGGAAGGTGTCAGCTACATACCATACAAAGATATCGTTGGCGTATGGACTGTATGTCACG
GACACACCGGAAAAGACATCATGCTCGGTAAAACGTATACCGAAGCAGAATGCAAAGCCCTCCTGAATAAAGACCTTGCC
ACGGTTGCCAGACAAATTAACCCGTACATCAAAGTCGATATACCGGAAACAACGCGCGGCGCTCTTTACTCGTTCGTCTA
CAACGTGGGTGCTGGCAATTTCAGAACATCGACGCTTCTTCGCAAAATAAACCAGGGCGATATCAAAGGCGCATGTGACC
AGCTACGTCGCTGGATATACGCTGGCGGTAAGCAATGGAAAGGCCTGATGACTCGTCGTGAGATTGAGCGTGAAGTCTGT
TTGTGGGGGCAACAATGA

Upstream 100 bases:

>100_bases
TAGGTGTGCTGGGTAGCCTGGTATTTGGCCTGCTGACGTACCTGACAAACCTTTATTTCAAGATTAAAGAAGATAAGCGC
AAGGCTGCGAGAGGTGAATA

Downstream 100 bases:

>100_bases
GCAGAGTAACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCGTGGGCGGTCAATCATTACCGTGAT
AACGCCATCGCCTACAAAGA

Product: bacteriophage lambda lysozyme-like protein

Products: NA

Alternate protein names: Endolysin; Lysis protein; Muramidase [H]

Number of amino acids: Translated: 165; Mature: 164

Protein sequence:

>165_residues
MPPSLRKAVAAAIGGGAIAIASVLITGPSGDDGLEGVSYIPYKDIVGVWTVCHGHTGKDIMLGKTYTEAECKALLNKDLA
TVARQINPYIKVDIPETTRGALYSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWIYAGGKQWKGLMTRREIEREVC
LWGQQ

Sequences:

>Translated_165_residues
MPPSLRKAVAAAIGGGAIAIASVLITGPSGDDGLEGVSYIPYKDIVGVWTVCHGHTGKDIMLGKTYTEAECKALLNKDLA
TVARQINPYIKVDIPETTRGALYSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWIYAGGKQWKGLMTRREIEREVC
LWGQQ
>Mature_164_residues
PPSLRKAVAAAIGGGAIAIASVLITGPSGDDGLEGVSYIPYKDIVGVWTVCHGHTGKDIMLGKTYTEAECKALLNKDLAT
VARQINPYIKVDIPETTRGALYSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWIYAGGKQWKGLMTRREIEREVCL
WGQQ

Specific function: Essential for lysis of bacterial cell wall, by showing cell wall hydrolyzing activity [H]

COG id: COG3772

COG function: function code R; Phage-related lysozyme (muraminidase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 24 family [H]

Homologues:

Organism=Escherichia coli, GI1786768, Length=165, Percent_Identity=98.1818181818182, Blast_Score=334, Evalue=1e-93,
Organism=Escherichia coli, GI1787836, Length=176, Percent_Identity=33.5227272727273, Blast_Score=76, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002196 [H]

Pfam domain/function: PF00959 Phage_lysozyme [H]

EC number: =3.2.1.17 [H]

Molecular weight: Translated: 17986; Mature: 17855

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPSLRKAVAAAIGGGAIAIASVLITGPSGDDGLEGVSYIPYKDIVGVWTVCHGHTGKDI
CCCHHHHHHHHHHCCCHHHHHHHHEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEE
MLGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGALYSFVYNVGAGNFRTSTLL
EECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCCHHHHHHH
RKINQGDIKGACDQLRRWIYAGGKQWKGLMTRREIEREVCLWGQQ
HHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PPSLRKAVAAAIGGGAIAIASVLITGPSGDDGLEGVSYIPYKDIVGVWTVCHGHTGKDI
CCHHHHHHHHHHCCCHHHHHHHHEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEE
MLGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGALYSFVYNVGAGNFRTSTLL
EECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCCHHHHHHH
RKINQGDIKGACDQLRRWIYAGGKQWKGLMTRREIEREVCLWGQQ
HHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]