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The map label for this gene is cdlD [C]

Identifier: 91213975

GI number: 91213975

Start: 4926598

End: 4927962

Strand: Direct

Name: cdlD [C]

Synonym: UTI89_C5027

Alternate gene names: 91213975

Gene position: 4926598-4927962 (Clockwise)

Preceding gene: 91213974

Following gene: 91213976

Centisome position: 97.25

GC content: 52.01

Gene sequence:

>1365_bases
ATGGTGGACATGATTAATGAAAGTGCACGGCAAACGCCAGTCATTGCACAAACGGACGTTCTGGTTATCGGGGGCGGTCC
GGCAGGATTATCCGCTGCCATTGCGGCAGGGCGGTTAGGTGCCAGAACCATGATTGTTGAGCGCTACGGGTCGCTAGGCG
GCGTATTGACGCAGGTCGGGGTAGAAAGTTTTGCCTGGTATCGTCATCCGGGGACGGAAGATTGTGAAGGGATCTGTCGT
GAGTATGAAGGCCGCGCACGAGCGCTGGGTTTCACACGACCAGAACCTCAGTCAATTAGCGAAGTTATAGATACTGAAGG
ATTTAAAGTTGTCGCCGATCAGATGATTACGGAATCTGGCGTTGAGCCGTTATATCACTCCTGGGTTGTGGACGTGATCA
AGGACGGGGATACGTTATGCGGTGTTATCGTCGAGAATAAATCAGGTCGAGGGGCAATTCTGGCGAAAAGAATCGTCGAT
TGCACGGGGGATGCTGATATTGCCGCTCGTGCAGGCGCGCCCTGGACGAAACGGAGCAAGGACCAACTGATGGGCGTCAC
CGTGATGTTCAGTTGCGCAGGTGTTGATGTGGCACGCTTTAACCGTTTTGTTGCGGAAGAACTTAAGCCGACCTACGCGG
ATTGGGGCAAAAACTGGACGATTCAAACCACGGGTAAAGAAGACCCGATGTTTAGCCCGTATATGGAGGATATTTTTACC
CGCGCGCAACAGGATGGTGTGATTCCAGGTGACGCCCAGGCGATTGCCGGAACCTGGTCGACCTTTTCTGAAAGCGGTGA
GGCTTTCCAGATGAATATGGTGTACGCCTTTGGTTTTGACTGTACCGATGTCTTCGATTTAACCAAAGCTGAGATTGCCG
GAAGGCAGCAAGCATTATGGGCAATTGACGCACTACGCCACTATGTTCCGGGCTTTGAAAATGTACGGTTACGCAATTTT
GGTGCCACGCTGGGGACGCGTGAATCACGGCTTATTGAGGGGGAAATACGTATTGCTGATGATTACGTCCTTAATCAGGG
GCGTTGTTCGGACAGTGTAGGGATTTTCCCGGAATTTATTGATGGTTCCGGTTATCTCATTTTGCCAACGACCGGGCGTT
TCTTTCAGATCCCTTATGGTTGTCTGGTGCCGCAAAAAGTGGAGAACCTTTTGGTCGCCGGTCGCTGTATTTCCGCAGGC
GTAGTTGCACATACTTCTATGCGTAACATGATGTGTTGTGCCGTTACCGGTGAGGCCGCAGGTACTGCCGCCGTGGTTTC
GCTACAGCAAAATTGCACCGTGCGTCAGGTTGCTATCCCTGATTTGCAAAACACGCTGCAACAGCAGGGCGTTCGTCTGG
CATAA

Upstream 100 bases:

>100_bases
CACTGGTCCTCCAGCGTGTGAATCGTAAAAGTAAAAATCAAGTCAATGAAATCACTCAAAACAATGCGGATTGCGCATCC
TGAATCTGACGGGGGAACCA

Downstream 100 bases:

>100_bases
CAACAAGGCGCAGAGAGCTGAAAGCCTGTCTGCGCCTTGCAGGAGAAAGACCATGAGGGATGATGACCTGGAGCACTTAA
GTGCCAATTACTGGATAACC

Product: putative dihydrolipoamide dehydrogenase CdlD

Products: lipoamide; NADH; acetyl-CoA; CO2; Proton; NH3; 5,10-methylene-THF; succinyl-CoA [C]

Alternate protein names: Invasion Protein IbeA; Glucose-Inhibited Division Protein A; Fumarate Reductase/Succinate Dehydrogenase Flavoprotein; Dihydrolipoamide Dehydrogenase; FAD-Dependent Pyridine Nucleotide-Disulfide Oxidoreductase; Invasion Protein IbeA Family Protein; Protein Involved In Invasion; FAD Dependent Oxidoreductase Domain Protein; Invasion Protein; Pyridine Nucleotide-Disulfide Oxidoreductase Family; tRNA Uridine 5-Carboxymethylaminomethyl GidA

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MVDMINESARQTPVIAQTDVLVIGGGPAGLSAAIAAGRLGARTMIVERYGSLGGVLTQVGVESFAWYRHPGTEDCEGICR
EYEGRARALGFTRPEPQSISEVIDTEGFKVVADQMITESGVEPLYHSWVVDVIKDGDTLCGVIVENKSGRGAILAKRIVD
CTGDADIAARAGAPWTKRSKDQLMGVTVMFSCAGVDVARFNRFVAEELKPTYADWGKNWTIQTTGKEDPMFSPYMEDIFT
RAQQDGVIPGDAQAIAGTWSTFSESGEAFQMNMVYAFGFDCTDVFDLTKAEIAGRQQALWAIDALRHYVPGFENVRLRNF
GATLGTRESRLIEGEIRIADDYVLNQGRCSDSVGIFPEFIDGSGYLILPTTGRFFQIPYGCLVPQKVENLLVAGRCISAG
VVAHTSMRNMMCCAVTGEAAGTAAVVSLQQNCTVRQVAIPDLQNTLQQQGVRLA

Sequences:

>Translated_454_residues
MVDMINESARQTPVIAQTDVLVIGGGPAGLSAAIAAGRLGARTMIVERYGSLGGVLTQVGVESFAWYRHPGTEDCEGICR
EYEGRARALGFTRPEPQSISEVIDTEGFKVVADQMITESGVEPLYHSWVVDVIKDGDTLCGVIVENKSGRGAILAKRIVD
CTGDADIAARAGAPWTKRSKDQLMGVTVMFSCAGVDVARFNRFVAEELKPTYADWGKNWTIQTTGKEDPMFSPYMEDIFT
RAQQDGVIPGDAQAIAGTWSTFSESGEAFQMNMVYAFGFDCTDVFDLTKAEIAGRQQALWAIDALRHYVPGFENVRLRNF
GATLGTRESRLIEGEIRIADDYVLNQGRCSDSVGIFPEFIDGSGYLILPTTGRFFQIPYGCLVPQKVENLLVAGRCISAG
VVAHTSMRNMMCCAVTGEAAGTAAVVSLQQNCTVRQVAIPDLQNTLQQQGVRLA
>Mature_454_residues
MVDMINESARQTPVIAQTDVLVIGGGPAGLSAAIAAGRLGARTMIVERYGSLGGVLTQVGVESFAWYRHPGTEDCEGICR
EYEGRARALGFTRPEPQSISEVIDTEGFKVVADQMITESGVEPLYHSWVVDVIKDGDTLCGVIVENKSGRGAILAKRIVD
CTGDADIAARAGAPWTKRSKDQLMGVTVMFSCAGVDVARFNRFVAEELKPTYADWGKNWTIQTTGKEDPMFSPYMEDIFT
RAQQDGVIPGDAQAIAGTWSTFSESGEAFQMNMVYAFGFDCTDVFDLTKAEIAGRQQALWAIDALRHYVPGFENVRLRNF
GATLGTRESRLIEGEIRIADDYVLNQGRCSDSVGIFPEFIDGSGYLILPTTGRFFQIPYGCLVPQKVENLLVAGRCISAG
VVAHTSMRNMMCCAVTGEAAGTAAVVSLQQNCTVRQVAIPDLQNTLQQQGVRLA

Specific function: Lipoamide Dehydrogenase Is A Component Of The Glycine Cleavage System As Well As Of The Alpha-Ketoacid Dehydrogenase Complexes. [C]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.8.1.4 [C]

Molecular weight: Translated: 49206; Mature: 49206

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDMINESARQTPVIAQTDVLVIGGGPAGLSAAIAAGRLGARTMIVERYGSLGGVLTQVG
CCCCCCCHHHCCCEEEECCEEEECCCCCCHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHH
VESFAWYRHPGTEDCEGICREYEGRARALGFTRPEPQSISEVIDTEGFKVVADQMITESG
HHHHHEECCCCCHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHC
VEPLYHSWVVDVIKDGDTLCGVIVENKSGRGAILAKRIVDCTGDADIAARAGAPWTKRSK
CCHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCH
DQLMGVTVMFSCAGVDVARFNRFVAEELKPTYADWGKNWTIQTTGKEDPMFSPYMEDIFT
HHHEEEEEEEEECCCCHHHHHHHHHHHCCCCHHHCCCCEEEEECCCCCCCCCHHHHHHHH
RAQQDGVIPGDAQAIAGTWSTFSESGEAFQMNMVYAFGFDCTDVFDLTKAEIAGRQQALW
HHHHCCCCCCCCHHHCCCHHHHHCCCCEEEEEEEEEECCCCHHHHHHHHHHHCCCHHHHH
AIDALRHYVPGFENVRLRNFGATLGTRESRLIEGEIRIADDYVLNQGRCSDSVGIFPEFI
HHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEECEEEEECHHEECCCCCCCCCCCCHHEE
DGSGYLILPTTGRFFQIPYGCLVPQKVENLLVAGRCISAGVVAHTSMRNMMCCAVTGEAA
CCCCEEEECCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCEEEEEEECCCC
GTAAVVSLQQNCTVRQVAIPDLQNTLQQQGVRLA
CCEEEEEECCCCEEEEEECCHHHHHHHHCCCCCC
>Mature Secondary Structure
MVDMINESARQTPVIAQTDVLVIGGGPAGLSAAIAAGRLGARTMIVERYGSLGGVLTQVG
CCCCCCCHHHCCCEEEECCEEEECCCCCCHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHH
VESFAWYRHPGTEDCEGICREYEGRARALGFTRPEPQSISEVIDTEGFKVVADQMITESG
HHHHHEECCCCCHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHC
VEPLYHSWVVDVIKDGDTLCGVIVENKSGRGAILAKRIVDCTGDADIAARAGAPWTKRSK
CCHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCH
DQLMGVTVMFSCAGVDVARFNRFVAEELKPTYADWGKNWTIQTTGKEDPMFSPYMEDIFT
HHHEEEEEEEEECCCCHHHHHHHHHHHCCCCHHHCCCCEEEEECCCCCCCCCHHHHHHHH
RAQQDGVIPGDAQAIAGTWSTFSESGEAFQMNMVYAFGFDCTDVFDLTKAEIAGRQQALW
HHHHCCCCCCCCHHHCCCHHHHHCCCCEEEEEEEEEECCCCHHHHHHHHHHHCCCHHHHH
AIDALRHYVPGFENVRLRNFGATLGTRESRLIEGEIRIADDYVLNQGRCSDSVGIFPEFI
HHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEECEEEEECHHEECCCCCCCCCCCCHHEE
DGSGYLILPTTGRFFQIPYGCLVPQKVENLLVAGRCISAGVVAHTSMRNMMCCAVTGEAA
CCCCEEEECCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCEEEEEEECCCC
GTAAVVSLQQNCTVRQVAIPDLQNTLQQQGVRLA
CCEEEEEECCCCEEEEEECCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADH [C]

Metal ions: NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {lipoamide}} 1.1 {NAD+}} [C]

Substrates: Dihydrolipoamide; NAD+; pyruvate; Coenzyme A; L-glycine; THF; 2-oxoglutarate [C]

Specific reaction: Dihydrolipoamide + NAD+ = lipoamide + NADH pyruvate + Coenzyme A + NAD = acetyl-CoA + CO2 + NADH + Proton NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF 2-oxoglutarate + Coenzyme A + NAD = succinyl-CoA + CO2 + NADH + Proton [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; p-Aminophenyl dichloroarsine [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA