| Definition | Escherichia coli UTI89 chromosome, complete genome. |
|---|---|
| Accession | NC_007946 |
| Length | 5,065,741 |
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The map label for this gene is amiB [H]
Identifier: 91213718
GI number: 91213718
Start: 4667862
End: 4669214
Strand: Direct
Name: amiB [H]
Synonym: UTI89_C4769
Alternate gene names: 91213718
Gene position: 4667862-4669214 (Clockwise)
Preceding gene: 91213717
Following gene: 91213719
Centisome position: 92.15
GC content: 54.55
Gene sequence:
>1353_bases TTGAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGTTGCTGCTGCTGTGCGCGCAGGTGGGTGC CGCGACGCTCTCTGATATTCAGGTTTCTAACGGCAACCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATT ATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTG TTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCT TACCGAAAATGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCCGATG TGCCGCCACCGCCTCCTCCGCCGCCTATGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAA CCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGC GCGCGCGACGGCTAACACAGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGATCCTGGCGCTATCG GCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATAACGATCCGATG TTTAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGC CAATTTCCTTGTATCGATTCATGCCGATGCCGCACCAAACCGTAGTGCCACCGGTGCCTCTGTATGGGTGCTCTCAAACC GTCGCGCCAACAGCGAAATGGCAAGCTGGCTGGAACAGCATGAGAAGCAGTCGGAGCTGCTGGGCGGGGCGGGTGATGTG CTGGCGAACAGTCAGTCTGACCCCTATTTAAGCCAGGCGGTGCTGGATTTACAGTTCGGTCATTCCCAGCGGGTAGGGTA TGATGTAGCGACCAGTATGATCAGTCAGTTGCAACGCATTGGCGAAATACATAAACGCCGACCAGAACACGCGAGTCTCG GCGTTCTGCGTTCGCCGGATATTCCGTCAGTACTGGTCGAAACCGGTTTTATCAGTAACAACAGCGAAGAACGTTTGCTG GCGAGCGACGATTACCAACAACAGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCA ATCTGCGCCGCAGGGTGCAACGGCACAAACGGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAA
Upstream 100 bases:
>100_bases CGAAATACACATTGATTATCAGGCACAAGGCCGTGAGGCGCGCGTGAGTGCGGTTTCCTCTGCGGGTGAATTGTTGCTGG CGCGTTTAGCCGGTTAACCT
Downstream 100 bases:
>100_bases GGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGC GTCGGTAGTCAAAGAACTGG
Product: N-acetylmuramoyl-l-alanine amidase II
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 450; Mature: 450
Protein sequence:
>450_residues MKGGGMMYRIRNWLVATLLLLCAQVGAATLSDIQVSNGNQQARITLSFIGDPDYAFSHQSKRTVALDIKQTGVIQGLPLL FSGNNLVKAIRSGTPKDAQTLRLVVDLTENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPMVAKRVETPAVVAPRVSE PARNPFKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNVTIAIARKLRTLLNNDPM FKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNRSATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDV LANSQSDPYLSQAVLDLQFGHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEERLL ASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN
Sequences:
>Translated_450_residues MKGGGMMYRIRNWLVATLLLLCAQVGAATLSDIQVSNGNQQARITLSFIGDPDYAFSHQSKRTVALDIKQTGVIQGLPLL FSGNNLVKAIRSGTPKDAQTLRLVVDLTENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPMVAKRVETPAVVAPRVSE PARNPFKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNVTIAIARKLRTLLNNDPM FKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNRSATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDV LANSQSDPYLSQAVLDLQFGHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEERLL ASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN >Mature_450_residues MKGGGMMYRIRNWLVATLLLLCAQVGAATLSDIQVSNGNQQARITLSFIGDPDYAFSHQSKRTVALDIKQTGVIQGLPLL FSGNNLVKAIRSGTPKDAQTLRLVVDLTENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPMVAKRVETPAVVAPRVSE PARNPFKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNVTIAIARKLRTLLNNDPM FKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNRSATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDV LANSQSDPYLSQAVLDLQFGHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEERLL ASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN
Specific function: Cell-wall hydrolase involved in septum cleavage during cell division. Can also act as powerful autolysin in the presence of murein synthesis inhibitors [H]
COG id: COG0860
COG function: function code M; N-acetylmuramoyl-L-alanine amidase
Gene ontology:
Cell location: Periplasm [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]
Homologues:
Organism=Escherichia coli, GI1790611, Length=445, Percent_Identity=99.1011235955056, Blast_Score=902, Evalue=0.0, Organism=Escherichia coli, GI87082163, Length=225, Percent_Identity=40.4444444444444, Blast_Score=177, Evalue=1e-45, Organism=Escherichia coli, GI1788776, Length=244, Percent_Identity=38.5245901639344, Blast_Score=174, Evalue=1e-44,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002508 [H]
Pfam domain/function: PF01520 Amidase_3 [H]
EC number: =3.5.1.28 [H]
Molecular weight: Translated: 48448; Mature: 48448
Theoretical pI: Translated: 9.97; Mature: 9.97
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.2 %Cys (Translated Protein) 2.0 %Met (Translated Protein) 2.2 %Cys+Met (Translated Protein) 0.2 %Cys (Mature Protein) 2.0 %Met (Mature Protein) 2.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKGGGMMYRIRNWLVATLLLLCAQVGAATLSDIQVSNGNQQARITLSFIGDPDYAFSHQS CCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHEEEECCCCCEEEEEEEEECCCCCCCCCCC KRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDLTENGKTEAVKRQN CCEEEEEEHHCCCCCCCCEEEECCHHHHHHHCCCCCCCEEEEEEEEECCCCCCHHHHHCC GSNYTVVFTINADVPPPPPPPPMVAKRVETPAVVAPRVSEPARNPFKTESNRTTGVISSN CCCEEEEEEEECCCCCCCCCCCHHHHHCCCCCEECCCCCCCCCCCCCCCCCCEEEEEECC TVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNVTIAIARKLRTLLNNDPM CCCCCHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCC FKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNRSATGASVWVLSNRRANSEM EEEEEECCCCEEEEEECCCHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCCHHH ASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQFGHSQRVGYDVATSMISQLQRI HHHHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH GEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEERLLASDDYQQQLAEAIYKGLRNY HHHHHCCCCCCCCCEEECCCCCCHHHEECCCCCCCCCCEECCCHHHHHHHHHHHHHHHHH FLAHPMQSAPQGATAQTASTVTTPDRTLPN HHHCCCCCCCCCCCCCCCCEECCCCCCCCC >Mature Secondary Structure MKGGGMMYRIRNWLVATLLLLCAQVGAATLSDIQVSNGNQQARITLSFIGDPDYAFSHQS CCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHEEEECCCCCEEEEEEEEECCCCCCCCCCC KRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDLTENGKTEAVKRQN CCEEEEEEHHCCCCCCCCEEEECCHHHHHHHCCCCCCCEEEEEEEEECCCCCCHHHHHCC GSNYTVVFTINADVPPPPPPPPMVAKRVETPAVVAPRVSEPARNPFKTESNRTTGVISSN CCCEEEEEEEECCCCCCCCCCCHHHHHCCCCCEECCCCCCCCCCCCCCCCCCEEEEEECC TVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNVTIAIARKLRTLLNNDPM CCCCCHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCC FKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNRSATGASVWVLSNRRANSEM EEEEEECCCCEEEEEECCCHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCCHHH ASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQFGHSQRVGYDVATSMISQLQRI HHHHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH GEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEERLLASDDYQQQLAEAIYKGLRNY HHHHHCCCCCCCCCEEECCCCCCHHHEECCCCCCCCCCEECCCHHHHHHHHHHHHHHHHH FLAHPMQSAPQGATAQTASTVTTPDRTLPN HHHCCCCCCCCCCCCCCCCEECCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 7511774; 7610040; 9278503; 1594459 [H]