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The map label for this gene is proP [H]

Identifier: 91213658

GI number: 91213658

Start: 4604211

End: 4605746

Strand: Direct

Name: proP [H]

Synonym: UTI89_C4705

Alternate gene names: 91213658

Gene position: 4604211-4605746 (Clockwise)

Preceding gene: 91213657

Following gene: 91213666

Centisome position: 90.89

GC content: 50.26

Gene sequence:

>1536_bases
TTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGAAAAAAGTAAAACCGATTACCCTTCGTGATGTCAC
CATTATTGATGACGGTAAACTGCGTAAAGCCATTACCGCAGCATCACTGGGTAATGCAATGGAATGGTTCGATTTTGGTG
TTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGGGGCTGACCCCAGCGTGCAGATGGTTGCTGCACTC
GCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGGCTCTTCTTTGGTATGTTGGGCGATAAATATGGTCGCCA
GAAGATCCTCGCTATCACTATTGTGATTATGTCGATCAGTACGTTCTGTATTGGCTTAATACCGTCCTACGACACGATTG
GTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGTCA
ATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTTGGTTCTATTGCCGGGTT
TGTGCTGGGAGCTGGCGTAGTGGTGTTAATTTCGACCATTGTCGGCGAAGAGAACTTCCTCGATTGGGGCTGGCGTATTC
CGTTCTTTATCGCTCTGCCGTTAGGGATTATCGGGCTTTACCTGCGCCATGCGCTGGAAGAGACTCCGGCGTTCCAGCAG
CATGTCGATAAACTGGAACAGGGCGACCGCGAAGGTTTGCAGGATGGCCCGAAAGTCTCGTTTAAAGAGATTGCCACCAA
ACACTGGCGCAGCCTGTTGACATGTATTGGTCTGGTAATTGCCACCAACGTGACTTACTACATGTTGCTGACCTATATGC
CGAGTTATTTGTCGCATAACCTGCATTACTCCGAAGACCACGGGGTGCTGATTATTATCGCCATTATGATCGGTATGCTG
TTTGTTCAACCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTTCTTGGTAGTGTTGCACTGTT
TGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGCTTATTGATGCTGGCGG
TGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCTGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTG
GCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAA
TCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGCGA
ATCGACCGTTGAAAGGTGCGACACCAGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTCGTCGAGCATTACGATAAT
ATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCC
GCGAATTGATGAATAA

Upstream 100 bases:

>100_bases
TAACCGGAGGGTGTAAGCAAACCCGCTACGCTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGT
CGCGCTGATTTTTCTGGCGT

Downstream 100 bases:

>100_bases
GCTGAAACGGATGGCCTGATGTGACGCTGTCTAATCAGGTCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCAT
ACTCTCCTCCAGGTTAACGG

Product: proline/glycine betaine transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MRKWANSARKAMLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVYGFVAYALGKVFFPGADPSVQMVAAL
ATFSVPFLIRPLGGLFFGMLGDKYGRQKILAITIVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGAS
IFVAEYSPDRKRGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEENFLDWGWRIPFFIALPLGIIGLYLRHALEETPAFQQ
HVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYYMLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGML
FVQPVMGLLSDRFGRRPFVLLGSVALFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSAL
AAAFNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGATPAASDIQEAKEILVEHYDN
IEQKIDDIDHEIADLQAKRTRLVQQHPRIDE

Sequences:

>Translated_511_residues
MRKWANSARKAMLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVYGFVAYALGKVFFPGADPSVQMVAAL
ATFSVPFLIRPLGGLFFGMLGDKYGRQKILAITIVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGAS
IFVAEYSPDRKRGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEENFLDWGWRIPFFIALPLGIIGLYLRHALEETPAFQQ
HVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYYMLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGML
FVQPVMGLLSDRFGRRPFVLLGSVALFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSAL
AAAFNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGATPAASDIQEAKEILVEHYDN
IEQKIDDIDHEIADLQAKRTRLVQQHPRIDE
>Mature_511_residues
MRKWANSARKAMLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVYGFVAYALGKVFFPGADPSVQMVAAL
ATFSVPFLIRPLGGLFFGMLGDKYGRQKILAITIVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGAS
IFVAEYSPDRKRGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEENFLDWGWRIPFFIALPLGIIGLYLRHALEETPAFQQ
HVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYYMLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGML
FVQPVMGLLSDRFGRRPFVLLGSVALFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSAL
AAAFNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGATPAASDIQEAKEILVEHYDN
IEQKIDDIDHEIADLQAKRTRLVQQHPRIDE

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=500, Percent_Identity=99.6, Blast_Score=1012, Evalue=0.0,
Organism=Escherichia coli, GI1788292, Length=443, Percent_Identity=31.8284424379233, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1789941, Length=452, Percent_Identity=33.1858407079646, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1788942, Length=440, Percent_Identity=29.7727272727273, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI87082231, Length=483, Percent_Identity=23.8095238095238, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI87082404, Length=389, Percent_Identity=24.6786632390745, Blast_Score=90, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6323512, Length=444, Percent_Identity=24.3243243243243, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 56179; Mature: 56179

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKWANSARKAMLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVYGFVAY
CCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHH
ALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAITIVIMSIS
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
TFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRKRGFMGSWLD
HHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHCCCCCCHHH
FGSIAGFVLGAGVVVLISTIVGEENFLDWGWRIPFFIALPLGIIGLYLRHALEETPAFQQ
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHH
HVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYYMLLTYMPSYLSHN
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVALFVLAIPAFILINSN
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC
VIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAAFNISVLVAGLTPTLAAW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHH
LVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGATPAASDIQEAKEILVEHYDN
HHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
IEQKIDDIDHEIADLQAKRTRLVQQHPRIDE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRKWANSARKAMLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVYGFVAY
CCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHH
ALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAITIVIMSIS
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
TFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRKRGFMGSWLD
HHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHCCCCCCHHH
FGSIAGFVLGAGVVVLISTIVGEENFLDWGWRIPFFIALPLGIIGLYLRHALEETPAFQQ
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHH
HVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYYMLLTYMPSYLSHN
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVALFVLAIPAFILINSN
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC
VIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAAFNISVLVAGLTPTLAAW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHH
LVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGATPAASDIQEAKEILVEHYDN
HHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
IEQKIDDIDHEIADLQAKRTRLVQQHPRIDE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]