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The map label for this gene is dnaB [H]

Identifier: 91213578

GI number: 91213578

Start: 4518902

End: 4520353

Strand: Direct

Name: dnaB [H]

Synonym: UTI89_C4625

Alternate gene names: 91213578

Gene position: 4518902-4520353 (Clockwise)

Preceding gene: 91213577

Following gene: 91213579

Centisome position: 89.21

GC content: 52.75

Gene sequence:

>1452_bases
TTGTCCCCCATTCAACAGCAGGTAACACCATTCGTTATGGCAGGCAATAGACCTTTCAACAAACAACAGACTGATAACCG
CGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCCTCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAA
TGCTGGATAACGAACGCTGGGATGATGTAGCTGAGCGTGTGGTGGCAGATGATTTTTACACCCGCCCACACCGTCATATC
TTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGTCCTATCGATCTGATTACCCTTGCGGAATCGCTGGAACGCCAGGG
GCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCGAACATCAGTGCAT
ATGCGGATATCGTGCGCGAACGTGCCGTTGTTCGTGAGATGATCTCCGTTGCTAACGAGATTGCCGAAGCTGGTTTTGAT
CCACAAGGGCGTACCAGTGAAGATCTGCTCGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAA
AGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACG
ATGGCGTTACCGGGGTAAATACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATC
GTTGCCGCGCGTCCGTCGATGGGTAAAACGACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACC
GGTGCTTATCTTTTCGCTGGAGATGCCATCAGAACAGATTATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGA
CTAAAATCCGTACCGGGCAGCTCGATGATGAAGACTGGGCACGCATTTCCGGCACCATGGGTATTTTGCTCGAAAAACGC
AATATCTATATCGATGATTCCTCCGGCTTGACGCCAACGGAAGTGCGTTCCCGCGCACGCCGTATTGCCCGTGAACACGG
CGGCATCGGGCTTATCATGATCGACTACCTGCAACTGATGCGCGTACCGGCGCTTTCCGATAACCGTACGCTGGAAATTG
CAGAAATCTCCCGCTCGCTGAAAGCACTGGCGAAAGAACTGAACGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCT
CTGGAACAACGTGCCGACAAACGCCCGGTCAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGAT
CATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAAC
GTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTAC
GACGACGAATAA

Upstream 100 bases:

>100_bases
CAGCCATTACATCTATCGCCCGACCCTGCAAATTCGCGCTTCCACCGGAAAGCGGGGGCGTTAAATGCTGTAGCGAGAAA
CTGTTTCTCGCTATACTCTC

Downstream 100 bases:

>100_bases
TAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACTAATGCAAGCGGCAACTGTTGTGATTAACC
GCCGCGCTCTGCGACACAAC

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MSPIQQQVTPFVMAGNRPFNKQQTDNRERDPQVAGLKVPPHSIEAEQSVLGGLMLDNERWDDVAERVVADDFYTRPHRHI
FTEMARLQESGSPIDLITLAESLERQGQLDSVGGFAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFD
PQGRTSEDLLDLAESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKTAGLQPSDLII
VAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASLSRVDQTKIRTGQLDDEDWARISGTMGILLEKR
NIYIDDSSGLTPTEVRSRARRIAREHGGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRS
LEQRADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVRLTFNGQWSRFDNYAGPQY
DDE

Sequences:

>Translated_483_residues
MSPIQQQVTPFVMAGNRPFNKQQTDNRERDPQVAGLKVPPHSIEAEQSVLGGLMLDNERWDDVAERVVADDFYTRPHRHI
FTEMARLQESGSPIDLITLAESLERQGQLDSVGGFAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFD
PQGRTSEDLLDLAESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKTAGLQPSDLII
VAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASLSRVDQTKIRTGQLDDEDWARISGTMGILLEKR
NIYIDDSSGLTPTEVRSRARRIAREHGGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRS
LEQRADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVRLTFNGQWSRFDNYAGPQY
DDE
>Mature_482_residues
SPIQQQVTPFVMAGNRPFNKQQTDNRERDPQVAGLKVPPHSIEAEQSVLGGLMLDNERWDDVAERVVADDFYTRPHRHIF
TEMARLQESGSPIDLITLAESLERQGQLDSVGGFAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDP
QGRTSEDLLDLAESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKTAGLQPSDLIIV
AARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASLSRVDQTKIRTGQLDDEDWARISGTMGILLEKRN
IYIDDSSGLTPTEVRSRARRIAREHGGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSL
EQRADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVRLTFNGQWSRFDNYAGPQYD
DE

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=471, Percent_Identity=99.1507430997877, Blast_Score=952, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 53808; Mature: 53677

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPIQQQVTPFVMAGNRPFNKQQTDNRERDPQVAGLKVPPHSIEAEQSVLGGLMLDNERW
CCCHHHHCCCEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHCCCEECCCHH
DDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGGFAYLAE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHH
LSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDLAESRVFKI
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
AESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKTAGLQPSDLII
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHCCCCCCCEEE
VAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASLSRVDQTKIRTGQ
EEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCC
LDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREHGGIGLIMIDYLQLM
CCCHHHHHHCCCEEEEEEECEEEEECCCCCCHHHHHHHHHHHHHHHCCEEEHHHHHHHHH
RVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQRADKRPVNSDLRESGSI
HCCCCCCCCEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCC
EQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVRLTFNGQWSRFDNYAGPQY
CCCCCEEEEEEEHHHHCCCCCHHHHHHHHHCCCCCCCEEEEEEEECCCHHHCCCCCCCCC
DDE
CCC
>Mature Secondary Structure 
SPIQQQVTPFVMAGNRPFNKQQTDNRERDPQVAGLKVPPHSIEAEQSVLGGLMLDNERW
CCHHHHCCCEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHCCCEECCCHH
DDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGGFAYLAE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHH
LSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDLAESRVFKI
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
AESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKTAGLQPSDLII
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHCCCCCCCEEE
VAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASLSRVDQTKIRTGQ
EEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCC
LDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREHGGIGLIMIDYLQLM
CCCHHHHHHCCCEEEEEEECEEEEECCCCCCHHHHHHHHHHHHHHHCCEEEHHHHHHHHH
RVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQRADKRPVNSDLRESGSI
HCCCCCCCCEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCC
EQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVRLTFNGQWSRFDNYAGPQY
CCCCCEEEEEEEHHHHCCCCCHHHHHHHHHCCCCCCCEEEEEEEECCCHHHCCCCCCCCC
DDE
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]