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The map label for this gene is purR [H]

Identifier: 91213577

GI number: 91213577

Start: 4517804

End: 4518865

Strand: Direct

Name: purR [H]

Synonym: UTI89_C4624

Alternate gene names: 91213577

Gene position: 4517804-4518865 (Clockwise)

Preceding gene: 91213571

Following gene: 91213578

Centisome position: 89.18

GC content: 50.28

Gene sequence:

>1062_bases
TTGATCTTATGGGGGTTTGGAGCAGGCATGGCGAAGTCGGATACAGGAAGAAAGCGGGTAACATTAACCGATGTTGCGCG
TGCGGCTGGCGTTTCGAAATCGACCGTATCGCTGGTTCTCAACGACAGTTCACTTATCAAAAAAGAGACTCAACAAAAAG
TCCAGCAAGCCATTGAACAATTAGGCTATGTCTATAACCGTTTTGCTGCCAATCTGCGTTCGCAAAAATCGCTAACTATC
GGCGTAGTCATTGACGATTTGATAAATCCTTTCTTCGCTGAATTTACTATGGGTCTTGAGATGACGCTGGCGGAGCACGG
TTTTATCACCGTGATGTCCAATACCTCCCAGCGTAGTGACCGGCAAAAACAGGTGCTTGATACCTTGCTGGAACACCACG
TCGCGGGCATTGTCCTCTGTCCAGTGAACAGTACCTCAGAAGCCGATTTACAGCGTTATGCCAACAGTTCCACACCCTTA
TTAATCACGATGCGCCCTCTGGATTGGCAACAATTGCCCGTCGATTACGTTGGCGTGGACAGCCACGCAGGTGTACGGGA
AGCGACGGAATATCTCATTCAACAAGGGCACAGAGATATCGTGTTTATTGGTGGTCTTACGCACCATATGCGTTATCAGG
GATACCTTGAGGCGATGAATCACCATGGTTTACAGCCGTGGAGTACTGATGCTTTTTCGCTGCGTGCTGAACCGACTCAG
GCCAACGGCTACCAGTTGATGCAACAGCTGTTGGATATGCCTTCGCCACCCACAGCGGTGATTTGTTATAACGATTTAAT
GGCTTTTGGCGCGGAGTCGGCACTAGGCGAAAGAGGATTATTTGCCGGGGAAGATATTTCGTTGATTGGTAATGATGGCG
TGGCGGCTTGTGCTTACAGTAACCCGCCGTTAACCACAATTGCGGTAGAACCATTGGCTCTGGGGAAACAGGCGGCGCAG
CAAATTTTACGGCGTATTGCGCAACCAGACGCACCGCTCAGCCATTACATCTATCGCCCGACCCTGCAAATTCGCGCTTC
CACCGGAAAGCGGGGGCGTTAA

Upstream 100 bases:

>100_bases
ACAAGGGAAGTGCGAATCTGTGATCGAAACGACGATCTCCTGCGATCCCTTGCGTTAACGGATCGCATGAGTAAAAGGAG
ATTATGATAAAGTAATTGCC

Downstream 100 bases:

>100_bases
ATGCTGTAGCGAGAAACTGTTTCTCGCTATACTCTCTTGTCCCCCATTCAACAGCAGGTAACACCATTCGTTATGGCAGG
CAATAGACCTTTCAACAAAC

Product: putative regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MILWGFGAGMAKSDTGRKRVTLTDVARAAGVSKSTVSLVLNDSSLIKKETQQKVQQAIEQLGYVYNRFAANLRSQKSLTI
GVVIDDLINPFFAEFTMGLEMTLAEHGFITVMSNTSQRSDRQKQVLDTLLEHHVAGIVLCPVNSTSEADLQRYANSSTPL
LITMRPLDWQQLPVDYVGVDSHAGVREATEYLIQQGHRDIVFIGGLTHHMRYQGYLEAMNHHGLQPWSTDAFSLRAEPTQ
ANGYQLMQQLLDMPSPPTAVICYNDLMAFGAESALGERGLFAGEDISLIGNDGVAACAYSNPPLTTIAVEPLALGKQAAQ
QILRRIAQPDAPLSHYIYRPTLQIRASTGKRGR

Sequences:

>Translated_353_residues
MILWGFGAGMAKSDTGRKRVTLTDVARAAGVSKSTVSLVLNDSSLIKKETQQKVQQAIEQLGYVYNRFAANLRSQKSLTI
GVVIDDLINPFFAEFTMGLEMTLAEHGFITVMSNTSQRSDRQKQVLDTLLEHHVAGIVLCPVNSTSEADLQRYANSSTPL
LITMRPLDWQQLPVDYVGVDSHAGVREATEYLIQQGHRDIVFIGGLTHHMRYQGYLEAMNHHGLQPWSTDAFSLRAEPTQ
ANGYQLMQQLLDMPSPPTAVICYNDLMAFGAESALGERGLFAGEDISLIGNDGVAACAYSNPPLTTIAVEPLALGKQAAQ
QILRRIAQPDAPLSHYIYRPTLQIRASTGKRGR
>Mature_353_residues
MILWGFGAGMAKSDTGRKRVTLTDVARAAGVSKSTVSLVLNDSSLIKKETQQKVQQAIEQLGYVYNRFAANLRSQKSLTI
GVVIDDLINPFFAEFTMGLEMTLAEHGFITVMSNTSQRSDRQKQVLDTLLEHHVAGIVLCPVNSTSEADLQRYANSSTPL
LITMRPLDWQQLPVDYVGVDSHAGVREATEYLIQQGHRDIVFIGGLTHHMRYQGYLEAMNHHGLQPWSTDAFSLRAEPTQ
ANGYQLMQQLLDMPSPPTAVICYNDLMAFGAESALGERGLFAGEDISLIGNDGVAACAYSNPPLTTIAVEPLALGKQAAQ
QILRRIAQPDAPLSHYIYRPTLQIRASTGKRGR

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=302, Percent_Identity=32.4503311258278, Blast_Score=173, Evalue=1e-44,
Organism=Escherichia coli, GI1790194, Length=333, Percent_Identity=33.9339339339339, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1787948, Length=320, Percent_Identity=31.5625, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1787906, Length=313, Percent_Identity=33.2268370607029, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1789202, Length=333, Percent_Identity=31.5315315315315, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1788474, Length=336, Percent_Identity=28.2738095238095, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1790715, Length=316, Percent_Identity=28.1645569620253, Blast_Score=127, Evalue=9e-31,
Organism=Escherichia coli, GI1786540, Length=314, Percent_Identity=30.2547770700637, Blast_Score=121, Evalue=9e-29,
Organism=Escherichia coli, GI1789068, Length=296, Percent_Identity=29.7297297297297, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI48994940, Length=314, Percent_Identity=27.3885350318471, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1787580, Length=323, Percent_Identity=25.6965944272446, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789456, Length=339, Percent_Identity=25.9587020648968, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1790689, Length=325, Percent_Identity=24.9230769230769, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1786268, Length=206, Percent_Identity=26.6990291262136, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38788; Mature: 38788

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILWGFGAGMAKSDTGRKRVTLTDVARAAGVSKSTVSLVLNDSSLIKKETQQKVQQAIEQ
CEEEEECCCCCCCCCCCEEEEHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHH
LGYVYNRFAANLRSQKSLTIGVVIDDLINPFFAEFTMGLEMTLAEHGFITVMSNTSQRSD
HHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHCCCEEEEECCCEEEEEECCCHHHH
RQKQVLDTLLEHHVAGIVLCPVNSTSEADLQRYANSSTPLLITMRPLDWQQLPVDYVGVD
HHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHCCCCCCEEEEECCCCHHHCCCEEECCC
SHAGVREATEYLIQQGHRDIVFIGGLTHHMRYQGYLEAMNHHGLQPWSTDAFSLRAEPTQ
CCCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCC
ANGYQLMQQLLDMPSPPTAVICYNDLMAFGAESALGERGLFAGEDISLIGNDGVAACAYS
CCHHHHHHHHHCCCCCCCEEEEEHHHHHHCCHHHCCCCCCCCCCCEEEEECCCEEEEEEC
NPPLTTIAVEPLALGKQAAQQILRRIAQPDAPLSHYIYRPTLQIRASTGKRGR
CCCEEEEEECHHHHHHHHHHHHHHHHCCCCCCHHHEEECCEEEEEECCCCCCC
>Mature Secondary Structure
MILWGFGAGMAKSDTGRKRVTLTDVARAAGVSKSTVSLVLNDSSLIKKETQQKVQQAIEQ
CEEEEECCCCCCCCCCCEEEEHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHH
LGYVYNRFAANLRSQKSLTIGVVIDDLINPFFAEFTMGLEMTLAEHGFITVMSNTSQRSD
HHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHCCCEEEEECCCEEEEEECCCHHHH
RQKQVLDTLLEHHVAGIVLCPVNSTSEADLQRYANSSTPLLITMRPLDWQQLPVDYVGVD
HHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHCCCCCCEEEEECCCCHHHCCCEEECCC
SHAGVREATEYLIQQGHRDIVFIGGLTHHMRYQGYLEAMNHHGLQPWSTDAFSLRAEPTQ
CCCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCC
ANGYQLMQQLLDMPSPPTAVICYNDLMAFGAESALGERGLFAGEDISLIGNDGVAACAYS
CCHHHHHHHHHCCCCCCCEEEEEHHHHHHCCHHHCCCCCCCCCCCEEEEECCCEEEEEEC
NPPLTTIAVEPLALGKQAAQQILRRIAQPDAPLSHYIYRPTLQIRASTGKRGR
CCCEEEEEECHHHHHHHHHHHHHHHHCCCCCCHHHEEECCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]