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The map label for this gene is yfkN [H]

Identifier: 91213487

GI number: 91213487

Start: 4414990

End: 4416549

Strand: Direct

Name: yfkN [H]

Synonym: UTI89_C4530

Alternate gene names: 91213487

Gene position: 4414990-4416549 (Clockwise)

Preceding gene: 91213482

Following gene: 91213488

Centisome position: 87.15

GC content: 47.88

Gene sequence:

>1560_bases
TTGTTTATGAAAATTAAAATACTTGCTGCGGGTATCGCATTAACACTGCCGTTCTGGGCTTGCGCTAAAGATGTCACTAT
TATTTATACCAACGACCTCCATGCGCATGTAGAACCTTATAAAGTGCCGTGGATCGCTGACGGTAAACGGGATATTGGCG
GCTGGGCAAATATCACTACGCTGGTTAAGCAAGAAAAAGCAAAAAATAAAGCGACATGGTTTTTTGATGCGGGCGACTAT
TTTACCGGGCCATATATCAGCAGCCTGACCAAAGGTAAGGCTATTATCGATATTATGAATACTATGCCGTTTGATGCCGT
CACAATAGGCAACCATGAATTTGACCACGGCTGGGACAACACATTATTACAGTTGAGCCAGGCAAAATTCCCTATTGTGC
AGGGCAATATTTTTTATCAGAACAGCAGTAAATCATTCTGGGATAAGCCCTATACCATCATCGAAAAAGACGGCGTGAAA
ATTGGCGTTATAGGTTTGCACGGAGTATTTGCCTTCAATGATACGGTATCTGCTGCCACGCGCGTGGGTATTGAAGCGCG
CGATGAAATTAAATGGCTACAACGTTATATCGATGAACTCAAAGGCAAGGTTGATTTGACCGTCGCCCTGATCCACGAAG
GTGTTCCAGCCCGCCAGTCCAGTATGGGAGGCACAGATGTGCGTCGCGCGTTGGATAAAGATATTCAGACGGCAAGTCAG
GTGAAAGGGCTGGATATTTTGATCACCGGACATGCACATGTGGGTACGCCGGAACCGATTAAAGTCGGCAATACGCTCAT
CCTCTCCACTGACAGCGGCGGGATTGATGTGGGCAAACTGGTTCTCGACTACAAAGAGAAGCCGCATAATTTTACGGTGA
AAAACTTCGAGCTTAAAACCATTTACGCTGACGAGTGGAAGCCCGATCCGCAAACGAAACAGGTGATCGACGGCTGGAAT
AAAAAGCTTGATGAAGTGGTGCAACAAACGGTGGCGCAATCGCCGGTTGAATTAAAACGTGCCTATGGTGAATCGGCTTC
CCTCGGGAACCTGGCGGCTGACGCTTTACTGGCAGCAGCGGGTAAAAATACCCAACTGGCGTTAACAAACTCTGGTGGTA
TCCGCAATGAGATCCCCGCAGGGGCGATTACGATGGGCGGCGTCATCAGTACGTTCCCGTTCCCCAACGAACTGGTGACG
ATGGAACTCACGGGTAAACAATTACGCAGTCTGATGGAACACGGCGCAAGTTTAAGCAATGGCGTTTTACAGGTATCGAA
AGGCCTGGAAATGAAGTACGACAGCAGCAAGCCGGTTGGTCAGCGGGTAATCACACTGACTCTGAATGGCAAACCCATTG
AAGATGCGACGGTTTATCACATTGCTACTCAGAGTTTCCTTGCTGATGGTGGAGATGGTTTTACCGCCTTTACCGAAGGG
AAAGCGCGTAACATAACGGGCGGTTATTACGTTTATCACGCGGTGGTTGATTACTTCAAAGCGGGCAACACCATTACGGA
TGAACAGCTCAACGGTATGCGCGTGAAGGATATCAAATAA

Upstream 100 bases:

>100_bases
TTGATTTTGTCCGGCAACTATCCCCTGCGGATGTGATTACTCTCACATAAATAAGTCTTTCAGAACGTAATGTAGCAATC
CTTTATCTCACTGAAAGGGA

Downstream 100 bases:

>100_bases
AAGTGCGCACTGAATTGACTCAACTGTTTGTTACCCGTTGATTGCGCAGATCCTGTATTCGGCCTACGCGAGGGCGCAGG
CCAAATCAGATGCCTTAACA

Product: hypothetical protein

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MFMKIKILAAGIALTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIADGKRDIGGWANITTLVKQEKAKNKATWFFDAGDY
FTGPYISSLTKGKAIIDIMNTMPFDAVTIGNHEFDHGWDNTLLQLSQAKFPIVQGNIFYQNSSKSFWDKPYTIIEKDGVK
IGVIGLHGVFAFNDTVSAATRVGIEARDEIKWLQRYIDELKGKVDLTVALIHEGVPARQSSMGGTDVRRALDKDIQTASQ
VKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDVGKLVLDYKEKPHNFTVKNFELKTIYADEWKPDPQTKQVIDGWN
KKLDEVVQQTVAQSPVELKRAYGESASLGNLAADALLAAAGKNTQLALTNSGGIRNEIPAGAITMGGVISTFPFPNELVT
MELTGKQLRSLMEHGASLSNGVLQVSKGLEMKYDSSKPVGQRVITLTLNGKPIEDATVYHIATQSFLADGGDGFTAFTEG
KARNITGGYYVYHAVVDYFKAGNTITDEQLNGMRVKDIK

Sequences:

>Translated_519_residues
MFMKIKILAAGIALTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIADGKRDIGGWANITTLVKQEKAKNKATWFFDAGDY
FTGPYISSLTKGKAIIDIMNTMPFDAVTIGNHEFDHGWDNTLLQLSQAKFPIVQGNIFYQNSSKSFWDKPYTIIEKDGVK
IGVIGLHGVFAFNDTVSAATRVGIEARDEIKWLQRYIDELKGKVDLTVALIHEGVPARQSSMGGTDVRRALDKDIQTASQ
VKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDVGKLVLDYKEKPHNFTVKNFELKTIYADEWKPDPQTKQVIDGWN
KKLDEVVQQTVAQSPVELKRAYGESASLGNLAADALLAAAGKNTQLALTNSGGIRNEIPAGAITMGGVISTFPFPNELVT
MELTGKQLRSLMEHGASLSNGVLQVSKGLEMKYDSSKPVGQRVITLTLNGKPIEDATVYHIATQSFLADGGDGFTAFTEG
KARNITGGYYVYHAVVDYFKAGNTITDEQLNGMRVKDIK
>Mature_519_residues
MFMKIKILAAGIALTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIADGKRDIGGWANITTLVKQEKAKNKATWFFDAGDY
FTGPYISSLTKGKAIIDIMNTMPFDAVTIGNHEFDHGWDNTLLQLSQAKFPIVQGNIFYQNSSKSFWDKPYTIIEKDGVK
IGVIGLHGVFAFNDTVSAATRVGIEARDEIKWLQRYIDELKGKVDLTVALIHEGVPARQSSMGGTDVRRALDKDIQTASQ
VKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDVGKLVLDYKEKPHNFTVKNFELKTIYADEWKPDPQTKQVIDGWN
KKLDEVVQQTVAQSPVELKRAYGESASLGNLAADALLAAAGKNTQLALTNSGGIRNEIPAGAITMGGVISTFPFPNELVT
MELTGKQLRSLMEHGASLSNGVLQVSKGLEMKYDSSKPVGQRVITLTLNGKPIEDATVYHIATQSFLADGGDGFTAFTEG
KARNITGGYYVYHAVVDYFKAGNTITDEQLNGMRVKDIK

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=529, Percent_Identity=26.2759924385633, Blast_Score=129, Evalue=1e-29,
Organism=Escherichia coli, GI1786687, Length=538, Percent_Identity=24.3494423791822, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI1790658, Length=560, Percent_Identity=22.5, Blast_Score=89, Evalue=8e-19,
Organism=Drosophila melanogaster, GI221329836, Length=540, Percent_Identity=26.8518518518519, Blast_Score=145, Evalue=7e-35,
Organism=Drosophila melanogaster, GI24641187, Length=514, Percent_Identity=27.431906614786, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI19921980, Length=487, Percent_Identity=25.6673511293634, Blast_Score=132, Evalue=8e-31,
Organism=Drosophila melanogaster, GI24652512, Length=487, Percent_Identity=25.6673511293634, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI161076508, Length=486, Percent_Identity=25.9259259259259, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI19922446, Length=528, Percent_Identity=25, Blast_Score=128, Evalue=9e-30,
Organism=Drosophila melanogaster, GI24654424, Length=528, Percent_Identity=25, Blast_Score=128, Evalue=9e-30,
Organism=Drosophila melanogaster, GI19922444, Length=542, Percent_Identity=24.7232472324723, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI28573524, Length=553, Percent_Identity=23.3273056057866, Blast_Score=123, Evalue=4e-28,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 56757; Mature: 56757

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS00786 5_NUCLEOTIDASE_2 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFMKIKILAAGIALTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIADGKRDIGGWANITT
CEEEEEEEECCHHHHCCHHHHCCCEEEEEECCCEECCCCEECCEEECCCCCCCCHHHHHH
LVKQEKAKNKATWFFDAGDYFTGPYISSLTKGKAIIDIMNTMPFDAVTIGNHEFDHGWDN
HHHHHHCCCCEEEEEECCCCCCCHHHHHHCCCCEEEEHHHCCCCCEEEECCCCCCCCCCH
TLLQLSQAKFPIVQGNIFYQNSSKSFWDKPYTIIEKDGVKIGVIGLHGVFAFNDTVSAAT
HHHHHHCCCCCEEECEEEEECCCCCCCCCCCEEEECCCCEEEEEEEEEEEEECCHHHHHH
RVGIEARDEIKWLQRYIDELKGKVDLTVALIHEGVPARQSSMGGTDVRRALDKDIQTASQ
HCCCCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHH
VKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDVGKLVLDYKEKPHNFTVKNFELKT
CCCEEEEEECCCCCCCCCCEECCCEEEEEECCCCCCHHHEEEECCCCCCCEEEEEEEEEE
IYADEWKPDPQTKQVIDGWNKKLDEVVQQTVAQSPVELKRAYGESASLGNLAADALLAAA
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHC
GKNTQLALTNSGGIRNEIPAGAITMGGVISTFPFPNELVTMELTGKQLRSLMEHGASLSN
CCCCEEEEECCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEEECHHHHHHHHHCCCCCCC
GVLQVSKGLEMKYDSSKPVGQRVITLTLNGKPIEDATVYHIATQSFLADGGDGFTAFTEG
CEEEECCCCEEEECCCCCCCCEEEEEEECCCCCCCCEEEEEEHHHHHCCCCCCCEEEECC
KARNITGGYYVYHAVVDYFKAGNTITDEQLNGMRVKDIK
CCCCCCCCCHHHHHHHHHHHCCCEECHHCCCCEEEECCC
>Mature Secondary Structure
MFMKIKILAAGIALTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIADGKRDIGGWANITT
CEEEEEEEECCHHHHCCHHHHCCCEEEEEECCCEECCCCEECCEEECCCCCCCCHHHHHH
LVKQEKAKNKATWFFDAGDYFTGPYISSLTKGKAIIDIMNTMPFDAVTIGNHEFDHGWDN
HHHHHHCCCCEEEEEECCCCCCCHHHHHHCCCCEEEEHHHCCCCCEEEECCCCCCCCCCH
TLLQLSQAKFPIVQGNIFYQNSSKSFWDKPYTIIEKDGVKIGVIGLHGVFAFNDTVSAAT
HHHHHHCCCCCEEECEEEEECCCCCCCCCCCEEEECCCCEEEEEEEEEEEEECCHHHHHH
RVGIEARDEIKWLQRYIDELKGKVDLTVALIHEGVPARQSSMGGTDVRRALDKDIQTASQ
HCCCCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHH
VKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDVGKLVLDYKEKPHNFTVKNFELKT
CCCEEEEEECCCCCCCCCCEECCCEEEEEECCCCCCHHHEEEECCCCCCCEEEEEEEEEE
IYADEWKPDPQTKQVIDGWNKKLDEVVQQTVAQSPVELKRAYGESASLGNLAADALLAAA
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHC
GKNTQLALTNSGGIRNEIPAGAITMGGVISTFPFPNELVTMELTGKQLRSLMEHGASLSN
CCCCEEEEECCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEEECHHHHHHHHHCCCCCCC
GVLQVSKGLEMKYDSSKPVGQRVITLTLNGKPIEDATVYHIATQSFLADGGDGFTAFTEG
CEEEECCCCEEEECCCCCCCCEEEEEEECCCCCCCCEEEEEEHHHHHCCCCCCCEEEECC
KARNITGGYYVYHAVVDYFKAGNTITDEQLNGMRVKDIK
CCCCCCCCCHHHHHHHHHHHCCCEECHHCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]