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The map label for this gene is yiaE [H]

Identifier: 91213066

GI number: 91213066

Start: 3984554

End: 3985540

Strand: Direct

Name: yiaE [H]

Synonym: UTI89_C4093

Alternate gene names: 91213066

Gene position: 3984554-3985540 (Clockwise)

Preceding gene: 91213065

Following gene: 91213068

Centisome position: 78.66

GC content: 51.67

Gene sequence:

>987_bases
ATGGAGAGAAGCATGAAGCCGTCCGTTATCCTCTACAAAGCCTTACCTGATGATTTACTGCAACGCCTGCAAGAACATTT
CACCGTTCACCAGGTGGCAAACCTCAGCCCACAAACCGTCGAACAAAATGCAGCAATTTTTGCCGAAGCTGAAGGCTTGC
TGGGTTCAAATGAGAATGTTGATGCCGCATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGC
TATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCTGATGCACACACCAACCGTATTAACAGAAACCGT
CGCCGATACGCTGATGGCGCTGGTGTTGTCTACCGCTCGTCGGGTTGTGGAGGTAGCAGAACGGGTAAAAGCAGGCGAAT
GGACCGCGAGCATAGGCCCGGACTGGTACGGCACTGACGTTCACCATAAAACACTGGGCATTGTCGGGATGGGACGGATC
GGTATGGCGCTGGCACAACGTGCGCACTTTGGTTTCAACATGCCCATCCTCTATAACGCGCGCCGCCACCATAAAGAAGC
AGAAGAACGCTTCAACGCCCGCTACTGCGATTTGGATACTCTGTTACAAGAGTCAGATTTCGTTTGCCTGATCCTGCCGT
TAACGGATGAGACGCATCATCTGTTTGGCGCAGAACAATTCGCCAAAATGAAATCCTCCGCCATTTTCATTAATGCCGGA
CGTGGCCCGGTGGTTGATGAAAATGCACTGATTGCTGCATTGCAGAAAGGGGAAATTCACGCCGCCGGGCTGGATGTCTT
CGAACAAGAGCCACTGTCCGTAGATTCGCCGTTGCTCTCAATGGCCAACGTCGTCGCAGTACCGCATATTGGATCTGCCA
CCCATGAGACGCGTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAAAAGAAC
TGTGTGAATCCGCACGTCGCGGACTAA

Upstream 100 bases:

>100_bases
CCACTGTAATCCCTTTCATGTCAGGTGATGCAGAAATCACCTGGCATTCTTCAGTTTTCCTTCATCATATTTCAGGCTAA
GGTGATCGCCTTATCAGTGA

Downstream 100 bases:

>100_bases
GCCGCGACTGCGTGGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACAGCTGTAGTCCAG
GCCTGATTAAACGCCTGATG

Product: putative dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MERSMKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFAEAEGLLGSNENVDAALLEKMPKLRATSTISVG
YDNFDVDALTARKILLMHTPTVLTETVADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRI
GMALAQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLFGAEQFAKMKSSAIFINAG
RGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLSMANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKN
CVNPHVAD

Sequences:

>Translated_328_residues
MERSMKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFAEAEGLLGSNENVDAALLEKMPKLRATSTISVG
YDNFDVDALTARKILLMHTPTVLTETVADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRI
GMALAQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLFGAEQFAKMKSSAIFINAG
RGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLSMANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKN
CVNPHVAD
>Mature_328_residues
MERSMKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFAEAEGLLGSNENVDAALLEKMPKLRATSTISVG
YDNFDVDALTARKILLMHTPTVLTETVADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRI
GMALAQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLFGAEQFAKMKSSAIFINAG
RGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLSMANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKN
CVNPHVAD

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrB subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=273, Percent_Identity=42.1245421245421, Blast_Score=191, Evalue=5e-49,
Organism=Homo sapiens, GI23308577, Length=312, Percent_Identity=30.7692307692308, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI145580578, Length=268, Percent_Identity=32.089552238806, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI4557499, Length=268, Percent_Identity=32.089552238806, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI145580575, Length=274, Percent_Identity=32.4817518248175, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI61743967, Length=270, Percent_Identity=32.2222222222222, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI4557497, Length=270, Percent_Identity=32.2222222222222, Blast_Score=122, Evalue=5e-28,
Organism=Escherichia coli, GI87082289, Length=324, Percent_Identity=99.6913580246914, Blast_Score=669, Evalue=0.0,
Organism=Escherichia coli, GI1787645, Length=269, Percent_Identity=27.5092936802974, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789279, Length=249, Percent_Identity=30.9236947791165, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI87081824, Length=258, Percent_Identity=27.5193798449612, Blast_Score=76, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17532191, Length=250, Percent_Identity=33.6, Blast_Score=142, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI25147481, Length=274, Percent_Identity=30.2919708029197, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324055, Length=243, Percent_Identity=38.6831275720165, Blast_Score=161, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6320925, Length=256, Percent_Identity=32.421875, Blast_Score=121, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6322116, Length=248, Percent_Identity=31.4516129032258, Blast_Score=113, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6325144, Length=198, Percent_Identity=31.3131313131313, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6324964, Length=240, Percent_Identity=27.0833333333333, Blast_Score=86, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6321253, Length=279, Percent_Identity=24.7311827956989, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI45551003, Length=312, Percent_Identity=39.1025641025641, Blast_Score=204, Evalue=9e-53,
Organism=Drosophila melanogaster, GI45552429, Length=312, Percent_Identity=39.1025641025641, Blast_Score=204, Evalue=9e-53,
Organism=Drosophila melanogaster, GI28574284, Length=312, Percent_Identity=39.1025641025641, Blast_Score=203, Evalue=1e-52,
Organism=Drosophila melanogaster, GI24585514, Length=312, Percent_Identity=39.1025641025641, Blast_Score=203, Evalue=1e-52,
Organism=Drosophila melanogaster, GI28574282, Length=312, Percent_Identity=39.1025641025641, Blast_Score=203, Evalue=1e-52,
Organism=Drosophila melanogaster, GI28574286, Length=315, Percent_Identity=37.7777777777778, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI28571528, Length=261, Percent_Identity=41.7624521072797, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24585516, Length=247, Percent_Identity=36.0323886639676, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24646446, Length=270, Percent_Identity=35.5555555555556, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24646448, Length=270, Percent_Identity=35.5555555555556, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24646452, Length=270, Percent_Identity=35.5555555555556, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24646450, Length=270, Percent_Identity=35.5555555555556, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI62472511, Length=270, Percent_Identity=35.5555555555556, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI19921140, Length=251, Percent_Identity=30.6772908366534, Blast_Score=119, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 35900; Mature: 35900

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERSMKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFAEAEGLLGSNENV
CCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCEEEEECCCCCCCCCCC
DAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTETVADTLMALVLSTAR
HHHHHHHCCHHCCCEEEEECCCCCCCHHHHHCEEEEEECCHHHHHHHHHHHHHHHHHHHH
RVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMALAQRAHFGFNMPILYNA
HHHHHHHHHHCCCEEECCCCCCCCCCCCHHHCCEEECCHHHHHHHHHHHCCCCCCEEECC
RRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLFGAEQFAKMKSSAIFINAG
HHHHHHHHHHHCCCCCCHHHHHCCCCCEEEEEECCCCCHHHHCHHHHHHHCCCEEEEECC
RGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLSMANVVAVPHIGSATHETRYG
CCCCCCCCHHEEEHHCCCEEECCCCHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHH
MAACAVDNLIDALQGKVEKNCVNPHVAD
HHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MERSMKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFAEAEGLLGSNENV
CCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCEEEEECCCCCCCCCCC
DAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTETVADTLMALVLSTAR
HHHHHHHCCHHCCCEEEEECCCCCCCHHHHHCEEEEEECCHHHHHHHHHHHHHHHHHHHH
RVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMALAQRAHFGFNMPILYNA
HHHHHHHHHHCCCEEECCCCCCCCCCCCHHHCCEEECCHHHHHHHHHHHCCCCCCEEECC
RRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLFGAEQFAKMKSSAIFINAG
HHHHHHHHHHHCCCCCCHHHHHCCCCCEEEEEECCCCCHHHHCHHHHHHHCCCEEEEECC
RGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLSMANVVAVPHIGSATHETRYG
CCCCCCCCHHEEEHHCCCEEECCCCHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHH
MAACAVDNLIDALQGKVEKNCVNPHVAD
HHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA