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The map label for this gene is manX [C]

Identifier: 91212944

GI number: 91212944

Start: 3861904

End: 3862236

Strand: Direct

Name: manX [C]

Synonym: UTI89_C3970

Alternate gene names: 91212944

Gene position: 3861904-3862236 (Clockwise)

Preceding gene: 91212942

Following gene: 91212945

Centisome position: 76.24

GC content: 38.14

Gene sequence:

>333_bases
ATGATTGTAGGTAAAACACCGCATACTACAGCTATTTCTCTGATGCCGGGAATGTCACCTGAAGCACTTATGCAACAAGT
AAAAACCATACTCAATCCTGATGTAGAAACAATCATATTTACGGATATTTATGGCGGCACACCTTCAAATATCGCCTACC
TTCTTTCACGAGAATTTCCCATTCGCTGCATCAGCGGTGTCAATCTGGCAATGTTAATTGAAGCCAACATGTTACAGGAT
TCTGACGAAGAACAGTCCTTTGATGAATATATTCAGCATATACATGATGCTGGTAAAGAGATGATTCAAACTTATTGTTT
TAATAAAGGATGA

Upstream 100 bases:

>100_bases
TCCTCTGTACATATTAAAAGGAATTTATCATTTGAACCAGTTCAATATTATAATTCTGACACACGGTGAGGCAGGGAATG
CTTTACTCGCCAGTAGCGAA

Downstream 100 bases:

>100_bases
TAAACTATGAGTATAGTACATGCCCGTATTGATTACCGTTTAATTCATGGACAAGTCATAACAAAATGGCTAAAAAGGAG
TGATGCAAATAAAATAATTG

Product: putative phosphotransferase system protein

Products: Protein Histidine; Sugar Phosphate.; D-glucosamine-6-phosphate [Cytoplasm]; pyruvate; glucose-6-phosphate [Cytoplasm]; N-acetyl-D-glucosamine-6-phosphate [Cytoplasm]; mannose-6-phosphate [Cytoplasm]; fructose-6-phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 110; Mature: 110

Protein sequence:

>110_residues
MIVGKTPHTTAISLMPGMSPEALMQQVKTILNPDVETIIFTDIYGGTPSNIAYLLSREFPIRCISGVNLAMLIEANMLQD
SDEEQSFDEYIQHIHDAGKEMIQTYCFNKG

Sequences:

>Translated_110_residues
MIVGKTPHTTAISLMPGMSPEALMQQVKTILNPDVETIIFTDIYGGTPSNIAYLLSREFPIRCISGVNLAMLIEANMLQD
SDEEQSFDEYIQHIHDAGKEMIQTYCFNKG
>Mature_110_residues
MIVGKTPHTTAISLMPGMSPEALMQQVKTILNPDVETIIFTDIYGGTPSNIAYLLSREFPIRCISGVNLAMLIEANMLQD
SDEEQSFDEYIQHIHDAGKEMIQTYCFNKG

Specific function: This Is A Component Of The Phosphoenolpyruvate-Dependent Sugar Phosphotransferase System (Pts), A Major Carbohydrate Active -Transport System. The IIcd Domains Contain The Sugar Binding Site And The Transmembrane Channel; The IIa Domain Contains The Prim

COG id: COG2893

COG function: function code G; Phosphotransferase system, mannose/fructose-specific component IIA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.1.69 [C]

Molecular weight: Translated: 12283; Mature: 12283

Theoretical pI: Translated: 4.29; Mature: 4.29

Prosite motif: PS51096 PTS_EIIA_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
6.4 %Met     (Translated Protein)
8.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
6.4 %Met     (Mature Protein)
8.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVGKTPHTTAISLMPGMSPEALMQQVKTILNPDVETIIFTDIYGGTPSNIAYLLSREFP
CCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHCCCCC
IRCISGVNLAMLIEANMLQDSDEEQSFDEYIQHIHDAGKEMIQTYCFNKG
CEEECCCCEEEEEECHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIVGKTPHTTAISLMPGMSPEALMQQVKTILNPDVETIIFTDIYGGTPSNIAYLLSREFP
CCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHCCCCC
IRCISGVNLAMLIEANMLQDSDEEQSFDEYIQHIHDAGKEMIQTYCFNKG
CEEECCCCEEEEEECHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein N-Phosphohistidine; Sugar; phosphoenolpyruvate; glucosamine [Periplasm]; phosphoenolpyruvate; beta-D-glucose [Periplasm]; N-acetyl-D-glucosamine [Periplasm]; mannose [Periplasm]; fructose [Periplasm] [C]

Specific reaction: Protein N-Phosphohistidine + Sugar = Protein Histidine + Sugar Phosphate. phosphoenolpyruvate + glucosamine [Periplasm] = D-glucosamine-6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + beta-D-glucose [Periplasm] = glucose-6-phosphate [Cytoplasm]

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA