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The map label for this gene is nrdE [H]

Identifier: 91212036

GI number: 91212036

Start: 2958077

End: 2960221

Strand: Direct

Name: nrdE [H]

Synonym: UTI89_C3035

Alternate gene names: 91212036

Gene position: 2958077-2960221 (Clockwise)

Preceding gene: 91212035

Following gene: 91212037

Centisome position: 58.39

GC content: 52.07

Gene sequence:

>2145_bases
TTGGCAACGACAACCGCAGAACGCCTGACGCAGGAAACGATGGATTATCACGCTCTGAATGCGATGCTTAACCTTTACGA
TAGCGCAGGTCGCATTCAGTTCGATAAAGACAGCCAGGCCGTAGACGCCTTTATGACGACGCATGTGCGTCCGAACAGTG
TGGCCTTCAGTAGCCAGCAGCAGCGCCTGAACTGGCTGGTTAACGAAGGTTACTATGATGAAAGCGTCCTTAATCGCTAC
TCTCGCGATTTTGTCATTACGCTGTTTGCCCACGCGCACGCCAGCGGTTTTCGTTTCCAGACATTCCTCGGAGCATGGAA
GTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAGCGTTATCTGGAAGATTTTGCCGATCGCGTCACGATGGTGG
CGCTGACGCTGGCACAAGGCGATGAAACACTGGCGACGCAGCTCACTGATGAGATGCTGTCAGGACGCTTTCAGCCAGCC
ACGCCAACATTCCTCAACTGCGGTAAGCAGCAGCGCGGCGAACTGGTTTCCTGTTTTCTGCTGCGTATTGAAGACAATAT
GGAGTCGATTGGCCGGGCAGTAAATTCCGCACTACAACTGTCGAAACGCGGCGGCGGCGTAGCATTTTTGCTGTCGAATC
TGCGGGAAGCGGGCGCGCCAATTAAACGTATTGAAAATCAATCTTCTGGCGTGATTCCGGTGATGAAAATGCTGGAAGAC
GCATTTTCCTATGCCAACCAACTCGGCGCGCGTCAGGGGGCTGGTGCAGTCTATTTACATGCTCATCATCCCGATATTCT
GCGTTTTCTCGATACAAAACGGGAAAATGCCGACGAAAAAATCCGCATTAAAACACTGTCGCTGGGCGTGGTGATCCCGG
ATATCACTTTCCATCTGGCAAAAGAGAATGCGCAGATGGCGCTGTTTTCGCCCTATGACGTAGAGCGAGTTTATGGCAAG
CCGTTTGCCGATGTCGCCATCAGTGAACACTATGACGAACTGGTTGCCGATGAACGCATCCGCAAAAAATACCTCAACGC
CCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGAGTCTGGCTATCCGTACATCATGTATGAAGACACGGTTAACC
GTGCTAACCCTATCGCCGGGCGCATAAATATGAGCAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAATAT
GACGAGAATCTCGACTATGCTCGTACAGGACATGATATTTCCTGCAATCTAGGGTCGTTGAATATTGCCCACACCATGGA
TTCCCCCGACTTTGCCCGCACGGTAGAGACTGCCGTACGCGGTTTAACGGCGGTGTCAGATATGAGTCATATCCGCAGCG
TGCCGTCCATCGAAGCCGGAAATGCCGCCTCACACGCCATCGGTCTGGGGCAGATGAATTTACACGGTTATCTGGCACGA
GAAGGCATCGCTTATGGGTCGCCGGAAGCACTGGATTTCACCAATCTCTATTTCTATACCATCACCTGGCACGCACTGCG
TACCTCGATGTTGCTGGCGCGCGAACGCGGTGAAACCTTCGCCGGGTTCAAACAGTCACGCTATGCCAGTGGTGAATATT
TCAGCCAATATCTGCAAGGTAACTGGCAGCCGAAAACGGCGAAAGTTGGCGAACTGTTTGCCCGTAGCGGTATTACGTTA
CCTACCCGTGAGATGTGGGCACAGCTGCGCGACGACGTGATGCGCTATGGTATCTACAACCAGAATCTCCAGGCGGTGCC
GCCAACCGGTTCTATCTCTTATATCAACCATGCCACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACGCAAAG
AGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAA
ATCGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGCTGTTTTT
CCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACGCTCTATTACA
TTCGCCTGCGTCAGATGGCGCTGGAAGGCACTGAAATTGAAGGCTGCGTCTCCTGTGCACTTTAA

Upstream 100 bases:

>100_bases
ATGTGATTGCCCGGAAATGCAGCGTGCCGTGGCTGTACCGCTTTGAACTCATGGGTACGCAAAGCGATATCGAAAACGTT
CGTAAAGGAGTAACCGAATT

Downstream 100 bases:

>100_bases
GGAATATCTATGAAACTCTCACGTATCAGCGCCATCAACTGGAACAAGATATCTGACGATAAAGATCTGGAGGTGTGGAA
TCGCCTGACCAGCAATTTCT

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: R1E protein; Ribonucleotide reductase 2 [H]

Number of amino acids: Translated: 714; Mature: 713

Protein sequence:

>714_residues
MATTTAERLTQETMDYHALNAMLNLYDSAGRIQFDKDSQAVDAFMTTHVRPNSVAFSSQQQRLNWLVNEGYYDESVLNRY
SRDFVITLFAHAHASGFRFQTFLGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLATQLTDEMLSGRFQPA
TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAGAPIKRIENQSSGVIPVMKMLED
AFSYANQLGARQGAGAVYLHAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGK
PFADVAISEHYDELVADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEILQVNSASEY
DENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSHIRSVPSIEAGNAASHAIGLGQMNLHGYLAR
EGIAYGSPEALDFTNLYFYTITWHALRTSMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITL
PTREMWAQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPAPFMTNENLALYQDAYE
IGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIYAWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL

Sequences:

>Translated_714_residues
MATTTAERLTQETMDYHALNAMLNLYDSAGRIQFDKDSQAVDAFMTTHVRPNSVAFSSQQQRLNWLVNEGYYDESVLNRY
SRDFVITLFAHAHASGFRFQTFLGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLATQLTDEMLSGRFQPA
TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAGAPIKRIENQSSGVIPVMKMLED
AFSYANQLGARQGAGAVYLHAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGK
PFADVAISEHYDELVADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEILQVNSASEY
DENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSHIRSVPSIEAGNAASHAIGLGQMNLHGYLAR
EGIAYGSPEALDFTNLYFYTITWHALRTSMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITL
PTREMWAQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPAPFMTNENLALYQDAYE
IGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIYAWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL
>Mature_713_residues
ATTTAERLTQETMDYHALNAMLNLYDSAGRIQFDKDSQAVDAFMTTHVRPNSVAFSSQQQRLNWLVNEGYYDESVLNRYS
RDFVITLFAHAHASGFRFQTFLGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLATQLTDEMLSGRFQPAT
PTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAGAPIKRIENQSSGVIPVMKMLEDA
FSYANQLGARQGAGAVYLHAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKP
FADVAISEHYDELVADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEILQVNSASEYD
ENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSHIRSVPSIEAGNAASHAIGLGQMNLHGYLARE
GIAYGSPEALDFTNLYFYTITWHALRTSMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLP
TREMWAQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPAPFMTNENLALYQDAYEI
GAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIYAWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R1E contains the binding sites for both substrates and allosteric effectors and carries out the actual reductio

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=601, Percent_Identity=27.9534109816972, Blast_Score=185, Evalue=1e-46,
Organism=Escherichia coli, GI1789030, Length=714, Percent_Identity=98.3193277310924, Blast_Score=1467, Evalue=0.0,
Organism=Escherichia coli, GI2367133, Length=582, Percent_Identity=25.7731958762887, Blast_Score=148, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17555242, Length=749, Percent_Identity=25.2336448598131, Blast_Score=180, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6320914, Length=619, Percent_Identity=26.0096930533118, Blast_Score=172, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6322123, Length=643, Percent_Identity=25.8164852255054, Blast_Score=168, Evalue=2e-42,
Organism=Drosophila melanogaster, GI17352481, Length=646, Percent_Identity=23.8390092879257, Blast_Score=150, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 80381; Mature: 80250

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATTTAERLTQETMDYHALNAMLNLYDSAGRIQFDKDSQAVDAFMTTHVRPNSVAFSSQQ
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHCCCCCCCCCCCCHH
QRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHASGFRFQTFLGAWKFYTSYTLKTFDGK
HHHHHHHCCCCCCHHHHHHHCCCEEEEEEEHHCCCCCCHHHHHHHHHHHHEEEEEECCCH
RYLEDFADRVTMVALTLAQGDETLATQLTDEMLSGRFQPATPTFLNCGKQQRGELVSCFL
HHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHCCHHHHHH
LRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAGAPIKRIENQSSGVIPVMKMLED
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHH
AFSYANQLGARQGAGAVYLHAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFHLA
HHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCEEEEE
KENAQMALFSPYDVERVYGKPFADVAISEHYDELVADERIRKKYLNARDFFQRLAEIQFE
CCCCCEEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCEEC
SGYPYIMYEDTVNRANPIAGRINMSNLCSEILQVNSASEYDENLDYARTGHDISCNLGSL
CCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHCCCCHHHCCCCEEECCCCE
NIAHTMDSPDFARTVETAVRGLTAVSDMSHIRSVPSIEAGNAASHAIGLGQMNLHGYLAR
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHEECCCCCCCHHHHH
EGIAYGSPEALDFTNLYFYTITWHALRTSMLLARERGETFAGFKQSRYASGEYFSQYLQG
CCCCCCCCCCEEECEEEEEEEEHHHHHHHHHHHHHCCCHHHCHHHHCCCCHHHHHHHHCC
NWQPKTAKVGELFARSGITLPTREMWAQLRDDVMRYGIYNQNLQAVPPTGSISYINHATS
CCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHCCC
SIHPIVAKVEIRKEGKTGRVYYPAPFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQG
CCCCEEEEEEEEECCCCCEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCC
LSLTLFFPDTATTRDINKAQIYAWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCC
>Mature Secondary Structure 
ATTTAERLTQETMDYHALNAMLNLYDSAGRIQFDKDSQAVDAFMTTHVRPNSVAFSSQQ
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHCCCCCCCCCCCCHH
QRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHASGFRFQTFLGAWKFYTSYTLKTFDGK
HHHHHHHCCCCCCHHHHHHHCCCEEEEEEEHHCCCCCCHHHHHHHHHHHHEEEEEECCCH
RYLEDFADRVTMVALTLAQGDETLATQLTDEMLSGRFQPATPTFLNCGKQQRGELVSCFL
HHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHCCHHHHHH
LRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAGAPIKRIENQSSGVIPVMKMLED
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHH
AFSYANQLGARQGAGAVYLHAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFHLA
HHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCEEEEE
KENAQMALFSPYDVERVYGKPFADVAISEHYDELVADERIRKKYLNARDFFQRLAEIQFE
CCCCCEEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCEEC
SGYPYIMYEDTVNRANPIAGRINMSNLCSEILQVNSASEYDENLDYARTGHDISCNLGSL
CCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHCCCCHHHCCCCEEECCCCE
NIAHTMDSPDFARTVETAVRGLTAVSDMSHIRSVPSIEAGNAASHAIGLGQMNLHGYLAR
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHEECCCCCCCHHHHH
EGIAYGSPEALDFTNLYFYTITWHALRTSMLLARERGETFAGFKQSRYASGEYFSQYLQG
CCCCCCCCCCEEECEEEEEEEEHHHHHHHHHHHHHCCCHHHCHHHHCCCCHHHHHHHHCC
NWQPKTAKVGELFARSGITLPTREMWAQLRDDVMRYGIYNQNLQAVPPTGSISYINHATS
CCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHCCC
SIHPIVAKVEIRKEGKTGRVYYPAPFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQG
CCCCEEEEEEEEECCCCCEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCC
LSLTLFFPDTATTRDINKAQIYAWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 8820648; 2158980 [H]