Click here to switch to the map view.

The map label for this gene is ygaF [H]

Identifier: 91212016

GI number: 91212016

Start: 2944775

End: 2946109

Strand: Direct

Name: ygaF [H]

Synonym: UTI89_C3015

Alternate gene names: 91212016

Gene position: 2944775-2946109 (Clockwise)

Preceding gene: 91212015

Following gene: 91212017

Centisome position: 58.13

GC content: 56.55

Gene sequence:

>1335_bases
GTGGCTATTTCGCTTACGCCACTCACCACTACCAGACGCATCAGTAAGCGCAAAGGAATTGAGCGGATGTATGATTTTTT
GATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTAATTGATGTTTATCCGGATGCCCGCATAGCGTTGC
TGGAAAAAGAGCCCGGCCCGGCCTGTCACCAGACGGGCCACAACAGTGGCGTAATCCATGCCGGGGTCTATTACACGCCC
GGCAGCCTGAAGGCGCAGTTTTGCCTGGCAGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGTATTCGCTACGA
CAACTGCGGCAAGATGCTGGTCGCCACGTCTGAACTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACGGCGGCGA
ACGGAATCGATCGCGAGTGGTTAAACGCCGAAGAGCTGCGCGAGCGCGAACCAAATATCACCGGGCTGGGCGGTATTTTT
GTGCCGTCCAGCGGCATTGTCAGCTACCGCGAAGTGACGGCGGCAATGGCAAAAATTTTTCAGGCCAGAGGCGGTGAGAT
TATCTATAACGCCGAAGTCAGCGCCCTCAGTGAGCATAAAAACGGCGTGGTGATATGTACCCGTCAGGGCGGTGAATATG
AAGCATCGACGCTGATTAGCTGTTCCGGGCTGATGGCTGACCGGCTGGTGAAAATGCTCGGCCTCGAACCGGGCTTTATT
ATCTGCCCGTTCCGCGGCGAGTATTTCCGCCTTGCGCCGGAGCATAACCAGATTGTTAACCACCTGATTTACCCCATTCC
CGACCCGGCAATGCCGTTTTTGGGCGTGCATCTCACCCGCATGATCGACGGCAGTGTGACCGTCGGGCCAAACGCGGTGC
TGGCTTTCAAACGCGAAGGCTATCGTAAGCGCGACTTCTCATTTAGCGACACGCTGGAAATTTTGGGCTCGTCGGGGATT
CGCCGGGTGCTGCAAAACCATCTACGCTCAGGACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCT
GGTGCAAAAGTATTGTCCCCGGCTTTCGTTAAGCGATCTCCAGCCCTGGCCCGCCGGTGTGCGGGCGCAGGCGGTATCGC
CGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGTAATGCGCCCTCCCCGGCA
GCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAGGTACAAACGCTGTTGGCAAGCCAGAGTAACCCCGGACG
CACGCTGCGAGCGGCACGTAGTGTGGATGCCTTACACGCCGCATTTAATCAATAA

Upstream 100 bases:

>100_bases
CCCGTTGGCAAATTCCTGTTGATTAACAACCTGTTCTGGCTGCACGGTCGCGACCGCTTTACTCCGCACCCGGATCTGCG
CCGTGAACTGATGCGTCAGC

Downstream 100 bases:

>100_bases
CCTTTGAAAACAGGATGTAGCGATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTTGATTAACGGGGAATGGC
TGGACGCCAACAATGGCGAG

Product: hydroxyglutarate oxidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MAISLTPLTTTRRISKRKGIERMYDFLIIGGGIIGMSTAMQLIDVYPDARIALLEKEPGPACHQTGHNSGVIHAGVYYTP
GSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERMRALWERTAANGIDREWLNAEELREREPNITGLGGIF
VPSSGIVSYREVTAAMAKIFQARGGEIIYNAEVSALSEHKNGVVICTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKREGYRKRDFSFSDTLEILGSSGI
RRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLSLSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPA
ATSAIPIGAHIVSKVQTLLASQSNPGRTLRAARSVDALHAAFNQ

Sequences:

>Translated_444_residues
MAISLTPLTTTRRISKRKGIERMYDFLIIGGGIIGMSTAMQLIDVYPDARIALLEKEPGPACHQTGHNSGVIHAGVYYTP
GSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERMRALWERTAANGIDREWLNAEELREREPNITGLGGIF
VPSSGIVSYREVTAAMAKIFQARGGEIIYNAEVSALSEHKNGVVICTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKREGYRKRDFSFSDTLEILGSSGI
RRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLSLSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPA
ATSAIPIGAHIVSKVQTLLASQSNPGRTLRAARSVDALHAAFNQ
>Mature_443_residues
AISLTPLTTTRRISKRKGIERMYDFLIIGGGIIGMSTAMQLIDVYPDARIALLEKEPGPACHQTGHNSGVIHAGVYYTPG
SLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERMRALWERTAANGIDREWLNAEELREREPNITGLGGIFV
PSSGIVSYREVTAAMAKIFQARGGEIIYNAEVSALSEHKNGVVICTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFII
CPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKREGYRKRDFSFSDTLEILGSSGIR
RVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLSLSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAA
TSAIPIGAHIVSKVQTLLASQSNPGRTLRAARSVDALHAAFNQ

Specific function: Oxidizes L-2-hydroxyglutarate, probably to recover alpha-ketoglutarate mistakenly reduced by other enzymes or formed during growth on propionate [H]

COG id: COG0579

COG function: function code R; Predicted dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the L2HGDH family [H]

Homologues:

Organism=Homo sapiens, GI13376331, Length=398, Percent_Identity=41.4572864321608, Blast_Score=318, Evalue=5e-87,
Organism=Escherichia coli, GI87082148, Length=422, Percent_Identity=97.39336492891, Blast_Score=838, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17565764, Length=401, Percent_Identity=42.643391521197, Blast_Score=328, Evalue=3e-90,
Organism=Drosophila melanogaster, GI24585081, Length=407, Percent_Identity=39.0663390663391, Blast_Score=281, Evalue=5e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015657
- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.1.3.15 [H]

Molecular weight: Translated: 48550; Mature: 48418

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAISLTPLTTTRRISKRKGIERMYDFLIIGGGIIGMSTAMQLIDVYPDARIALLEKEPGP
CEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCC
ACHQTGHNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELE
HHHCCCCCCCEEEEEEEECCCCCEEEEEEECCCHHHHHHCCCCCEECCCCCEEEEEHHHH
MERMRALWERTAANGIDREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIF
HHHHHHHHHHHHCCCCCHHHCCHHHHHHCCCCCEECCEEECCCCCCHHHHHHHHHHHHHH
QARGGEIIYNAEVSALSEHKNGVVICTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
HHCCCCEEEECCHHHHHHCCCCEEEEECCCCCCCHHHHEEHHHHHHHHHHHHHCCCCCEE
ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKREG
EECCCCCEEEECCCHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEECCCEEEEEECCC
YRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLSLSDL
CCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
QPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSKVQTLLA
CCCCCCCEEEEECCCCCEEHHEEEEECCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
SQSNPGRTLRAARSVDALHAAFNQ
CCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AISLTPLTTTRRISKRKGIERMYDFLIIGGGIIGMSTAMQLIDVYPDARIALLEKEPGP
EEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCC
ACHQTGHNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELE
HHHCCCCCCCEEEEEEEECCCCCEEEEEEECCCHHHHHHCCCCCEECCCCCEEEEEHHHH
MERMRALWERTAANGIDREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIF
HHHHHHHHHHHHCCCCCHHHCCHHHHHHCCCCCEECCEEECCCCCCHHHHHHHHHHHHHH
QARGGEIIYNAEVSALSEHKNGVVICTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
HHCCCCEEEECCHHHHHHCCCCEEEEECCCCCCCHHHHEEHHHHHHHHHHHHHCCCCCEE
ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKREG
EECCCCCEEEECCCHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEECCCEEEEEECCC
YRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLSLSDL
CCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
QPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSKVQTLLA
CCCCCCCEEEEECCCCCEEHHEEEEECCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
SQSNPGRTLRAARSVDALHAAFNQ
CCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 8297211; 7984428 [H]