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The map label for this gene is pfkB [H]

Identifier: 91210937

GI number: 91210937

Start: 1841081

End: 1842013

Strand: Direct

Name: pfkB [H]

Synonym: UTI89_C1916

Alternate gene names: 91210937

Gene position: 1841081-1842013 (Clockwise)

Preceding gene: 91210935

Following gene: 91210939

Centisome position: 36.34

GC content: 53.48

Gene sequence:

>933_bases
ATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCACTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGG
AAAACTGCGCTGTACCGCACCGGTATTCGAACCCGGAGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAG
GCAGTGCCACAGCGATCTTCCCGGCGGGTGGTGCGACCGGCGAACATCTGGTTTCACTGTTGGCAGATGAAAATGTCCCC
GTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTT
TGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCA
TTCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGCTGGAAAAATTAACCCAACTGATTTCCGCTGCGCAAAAACAA
GGGATCCGCTGCATCATCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCGATTGGTAACATCGAGTTGGTTAAGCC
TAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCAGACGATGTCCGCAAAGCCGCGCAGGAAATTG
TTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGGGCGTTGGGCGTTGATAGTGAAAACTGCATT
CAGGTGGTGCCACCACCGGTAAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACT
GGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACAC
GCCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAA

Upstream 100 bases:

>100_bases
TTATCTGCAAAATTTTAAGTAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTG
AAATCAGCCTATAGGAGGAA

Downstream 100 bases:

>100_bases
CAAAAACATTCCCCCTGCATTGGGGGAATCACCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATG
TGATAACAAAGGGGTGAACT

Product: 6-phosphofructokinase 2

Products: NA

Alternate protein names: Phosphofructokinase-2 [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MMVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINVARAIAHLGGSATAIFPAGGATGEHLVSLLADENVP
VATVEAKDWTRQNLHVHVEASGEQYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQKQ
GIRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKAKRVVVSLGPQGALGVDSENCI
QVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMVRFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR

Sequences:

>Translated_310_residues
MMVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINVARAIAHLGGSATAIFPAGGATGEHLVSLLADENVP
VATVEAKDWTRQNLHVHVEASGEQYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQKQ
GIRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKAKRVVVSLGPQGALGVDSENCI
QVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMVRFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR
>Mature_310_residues
MMVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINVARAIAHLGGSATAIFPAGGATGEHLVSLLADENVP
VATVEAKDWTRQNLHVHVEASGEQYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQKQ
GIRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKAKRVVVSLGPQGALGVDSENCI
QVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMVRFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR

Specific function: Key control step of glycolysis. [C]

COG id: COG1105

COG function: function code G; Fructose-1-phosphate kinase and related fructose-6-phosphate kinase (PfkB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Escherichia coli, GI1788017, Length=309, Percent_Identity=99.6763754045307, Blast_Score=626, Evalue=0.0,
Organism=Escherichia coli, GI1788493, Length=287, Percent_Identity=33.7979094076655, Blast_Score=105, Evalue=3e-24,

Paralogues:

None

Copy number: 496 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR017583 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 32602; Mature: 32602

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00583 PFKB_KINASES_1 ; PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINVARAIAHLGGSATAIF
CEEEEEEEEECCCCCCCEECCEECCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCCEEEE
PAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGEQYRFVMPGAALNEDEFR
ECCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEEECCCEEEEEECCCCCCHHHHH
QLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQKQGIRCIIDSSGEALSAALAIG
HHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEEEC
NIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKAKRVVVSLGPQGALGVDSENCI
CEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCE
QVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMVRFGVAAGSAATLNQGTRLCSHDD
EECCCCCCCCCCCCCCCCHHEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCC
TQKIYAYLSR
HHHHHHHHCC
>Mature Secondary Structure
MMVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINVARAIAHLGGSATAIF
CEEEEEEEEECCCCCCCEECCEECCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCCEEEE
PAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGEQYRFVMPGAALNEDEFR
ECCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEEECCCEEEEEECCCCCCHHHHH
QLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQKQGIRCIIDSSGEALSAALAIG
HHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEEEC
NIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKAKRVVVSLGPQGALGVDSENCI
CEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCE
QVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMVRFGVAAGSAATLNQGTRLCSHDD
EECCCCCCCCCCCCCCCCHHEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCC
TQKIYAYLSR
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6235149; 9097039; 9278503; 6310120 [H]