| Definition | Escherichia coli UTI89 chromosome, complete genome. |
|---|---|
| Accession | NC_007946 |
| Length | 5,065,741 |
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The map label for this gene is topA [H]
Identifier: 91210566
GI number: 91210566
Start: 1451953
End: 1454592
Strand: Direct
Name: topA [H]
Synonym: UTI89_C1543
Alternate gene names: 91210566
Gene position: 1451953-1454592 (Clockwise)
Preceding gene: 91210565
Following gene: 91210567
Centisome position: 28.66
GC content: 51.74
Gene sequence:
>2640_bases GTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCTCTTGTCATCGTTGAGTCCCCGGCAAA AGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTTGGTCACATCCGCGATTTGCCGACCA GTGGCTCAACTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCT CTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGTTGCCTGGTAAAGAGAAGGTCGT CTCTGAACTGAAACAACTGGCTGAAAAAGCCGACCACATCTATCTCGCAACCGACCTTGACCGCGAAGGGGAAGCCATTG CATGGCACCTGCGGGAAGTGATTGGTGGTGATGATGCGCGCTATAGCCGAGTGGTGTTTAACGAAATTACTAAAAACGCG ATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCG CGTGGTGGGGTATATGGTTTCGCCGCTTCTATGGAAAAAGATCGCTCGTGGTCTGTCTGCCGGTCGTGTGCAGTCGGTGG CGGTCCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTACCGGAAGAGTTCTGGGAAGTCGATGCCAGCACG ACCACGCCATCTGGTGAAGCGTTGGCGTTGCAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACA AACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAAC CTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGAAAAAAACCATGATG ATGGCTCAGCGTTTGTATGAAGCAGGCTATATCACTTACATGCGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAA TATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATATCTGCCGGAAAGTCCGAATCAGTACGCCAGCAAAGAAA ACTCACAGGAAGCGCACGAAGCGATTCGTCCTTCTGACGTCAATGTGATGGCGGAATCGCTGAAGGATATGGAAGCAGAT GCGCAGAAACTGTACCAGTTAATCTGGCGTCAGTTCGTTGCCTGCCAGATGACCCCAGCGAAATATGACTCCACGACGCT GACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGCTTTGATGGCTGGACGAAAGTGATGCCTG CACTGCGTAAAGGCGATGAAGATCGTATCTTACCGGCAGTCGATAAAGGCGATGCTCTGACGCTCGTTGAACTGACACCA GCCCAGCACTTTACCAAGCCTCCAGCCCGTTTCAGTGAAGCATCGCTGGTCAAAGAGCTGGAAAAACGCGGTATCGGTCG TCCTTCTACCTATGCGTCGATCATTTCGACTATTCAGGATCGTGGCTATGTGCGAGTAGAAAATCGTCGTTTCTATGCGG AAAAAATGGGCGAAATCGTCACCGATCGCCTGGAAGAGAATTTCCGCGAGTTAATGAACTACGACTTCACCGCGCAGATG GAAAACAGCCTCGACCAGGTGGCAAATCACGAAGCAGAGTGGAAAGCTGTACTGGATCACTTCTTCTCGGATTTCACCCA GCAGTTAGATAAAGCTGAAAAAGATCCGGAAGAGGGTGGTATGCGCCCGAACCAGATGGTTCTGACCAGCATTGACTGCC CGACTTGTGGTCGCAAAATGGGGATTCGCACAGCGAGCACCGGGGTATTCCTTGGCTGTTCTGGCTATGCGCTGCCGCCG AAAGAGCGTTGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGACGCTGAAAC CAATGCGTTGCGCGCAAAACGTCGTTGCCCGAAATGCGGTACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGT TGCATGTCTGTGGTAATAACCCTACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGC CCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAA CGAAGAGTGTAAAAACACACGTAAGATTTTACGTAACGGCGAAGTGGCACCACCGAAAGAAGATCCGGTGCCATTACCGG AGCTGCCGTGCGAAAAATCAGATGCCTATTTCGTGTTGCGTGACGGTGCTGCCGGTGTGTTCCTGGCGGCCAATACCTTC CCGAAATCGCGTGAAACGCGTGCGCCGCTGGTGGAGGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTA TCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATG TCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGGAAAAAATAA
Upstream 100 bases:
>100_bases AGTCACCTGAATTTTCGTGAGCAGAGTCACGACAAGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTG CCAGTGGAAGGTTTATCAAC
Downstream 100 bases:
>100_bases CCTTTAATTCTGTCTGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACAC CTATACACTAAGGCTATAAA
Product: DNA topoisomerase I
Products: NA
Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]
Number of amino acids: Translated: 879; Mature: 879
Protein sequence:
>879_residues MRRIPGRIKLGKVNMGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSTAKKSADSTSTKTAKKPKKDERGA LVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEKADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNA IRQAFNKPGELNIDRVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEEFWEVDAST TTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPTTSKPGAPFITSTLQQAASTRLGFGVKKTMM MAQRLYEAGYITYMRTDSTNLSQDAVNMVRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEAD AQKLYQLIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRILPAVDKGDALTLVELTP AQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQM ENSLDQVANHEAEWKAVLDHFFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYALPP KERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHVCGNNPTCDGYEIEEGEFRIKGYDG PIVECEKCGSEMHLKMGRFGKYMACTNEECKNTRKILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTF PKSRETRAPLVEELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYVDGKWVEGKK
Sequences:
>Translated_879_residues MRRIPGRIKLGKVNMGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSTAKKSADSTSTKTAKKPKKDERGA LVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEKADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNA IRQAFNKPGELNIDRVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEEFWEVDAST TTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPTTSKPGAPFITSTLQQAASTRLGFGVKKTMM MAQRLYEAGYITYMRTDSTNLSQDAVNMVRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEAD AQKLYQLIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRILPAVDKGDALTLVELTP AQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQM ENSLDQVANHEAEWKAVLDHFFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYALPP KERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHVCGNNPTCDGYEIEEGEFRIKGYDG PIVECEKCGSEMHLKMGRFGKYMACTNEECKNTRKILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTF PKSRETRAPLVEELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYVDGKWVEGKK >Mature_879_residues MRRIPGRIKLGKVNMGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSTAKKSADSTSTKTAKKPKKDERGA LVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEKADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNA IRQAFNKPGELNIDRVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEEFWEVDAST TTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPTTSKPGAPFITSTLQQAASTRLGFGVKKTMM MAQRLYEAGYITYMRTDSTNLSQDAVNMVRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEAD AQKLYQLIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRILPAVDKGDALTLVELTP AQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQM ENSLDQVANHEAEWKAVLDHFFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYALPP KERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHVCGNNPTCDGYEIEEGEFRIKGYDG PIVECEKCGSEMHLKMGRFGKYMACTNEECKNTRKILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTF PKSRETRAPLVEELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYVDGKWVEGKK
Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]
COG id: COG0550
COG function: function code L; Topoisomerase IA
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 Toprim domain [H]
Homologues:
Organism=Homo sapiens, GI10835218, Length=609, Percent_Identity=25.2873563218391, Blast_Score=148, Evalue=2e-35, Organism=Homo sapiens, GI4507635, Length=514, Percent_Identity=27.431906614786, Blast_Score=148, Evalue=3e-35, Organism=Escherichia coli, GI1787529, Length=865, Percent_Identity=99.7687861271676, Blast_Score=1801, Evalue=0.0, Organism=Escherichia coli, GI1788061, Length=549, Percent_Identity=23.6794171220401, Blast_Score=122, Evalue=9e-29, Organism=Caenorhabditis elegans, GI17555378, Length=505, Percent_Identity=25.5445544554455, Blast_Score=130, Evalue=4e-30, Organism=Caenorhabditis elegans, GI32563869, Length=565, Percent_Identity=24.4247787610619, Blast_Score=127, Evalue=2e-29, Organism=Drosophila melanogaster, GI24585251, Length=618, Percent_Identity=24.2718446601942, Blast_Score=137, Evalue=4e-32, Organism=Drosophila melanogaster, GI24640096, Length=508, Percent_Identity=25.9842519685039, Blast_Score=122, Evalue=1e-27,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR003601 - InterPro: IPR013497 - InterPro: IPR013824 - InterPro: IPR013825 - InterPro: IPR000380 - InterPro: IPR003602 - InterPro: IPR013498 - InterPro: IPR005733 - InterPro: IPR013263 - InterPro: IPR006171 [H]
Pfam domain/function: PF08272 Topo_Zn_Ribbon; PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]
EC number: =5.99.1.2 [H]
Molecular weight: Translated: 99002; Mature: 99002
Theoretical pI: Translated: 8.96; Mature: 8.96
Prosite motif: PS00396 TOPOISOMERASE_I_PROK
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.6 %Cys (Translated Protein) 2.8 %Met (Translated Protein) 4.4 %Cys+Met (Translated Protein) 1.6 %Cys (Mature Protein) 2.8 %Met (Mature Protein) 4.4 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MRRIPGRIKLGKVNMGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSTAKK CCCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHHCCCEEEECCCCHHHCCCCCCCCHHC SADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEKADHI CCCCCCCHHCCCCCCCHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCEE YLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNIDRVNAQQ EEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHH ARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEEFWEVDAST HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCC TTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPTTSKPGAPFIT CCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH STLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNMVRGYISDNFGKK HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCC YLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQLIWRQFVACQMTPA CCCCCCCHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC KYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRILPAVDKGDALTLVELTP CCCCCEEEECCCCEEEEECCCEEEECCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECC AQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIV HHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHH TDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDHFFSDFTQQLDKAEKDPEEGG HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCC MRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYALPPKERCKTTINLVPENEVLNVL CCCCCEEEEECCCCCCCHHHCCEEECCCEEEECCCCCCCCHHHCCCEEEECCCHHHHHHH EGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHVCGNNPTCDGYEIEEGEFRIKGYDG CCCCCHHHHHHHHHCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCEEECCCCEEEECCCC PIVECEKCGSEMHLKMGRFGKYMACTNEECKNTRKILRNGEVAPPKEDPVPLPELPCEKS CCHHHHHCCCHHHHHHHCCCCEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC DAYFVLRDGAAGVFLAANTFPKSRETRAPLVEELYRFRDRLPEKLRYLADAPQQDPEGNK CCEEEEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH TMVRFSRKTKQQYVSSEKDGKATGWSAFYVDGKWVEGKK HHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCEECCCCC >Mature Secondary Structure MRRIPGRIKLGKVNMGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSTAKK CCCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHHCCCEEEECCCCHHHCCCCCCCCHHC SADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEKADHI CCCCCCCHHCCCCCCCHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCEE YLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNIDRVNAQQ EEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHH ARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEEFWEVDAST HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCC TTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPTTSKPGAPFIT CCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH STLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNMVRGYISDNFGKK HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCC YLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQLIWRQFVACQMTPA CCCCCCCHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC KYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRILPAVDKGDALTLVELTP CCCCCEEEECCCCEEEEECCCEEEECCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECC AQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIV HHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHH TDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDHFFSDFTQQLDKAEKDPEEGG HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCC MRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYALPPKERCKTTINLVPENEVLNVL CCCCCEEEEECCCCCCCHHHCCEEECCCEEEECCCCCCCCHHHCCCEEEECCCHHHHHHH EGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHVCGNNPTCDGYEIEEGEFRIKGYDG CCCCCHHHHHHHHHCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCEEECCCCEEEECCCC PIVECEKCGSEMHLKMGRFGKYMACTNEECKNTRKILRNGEVAPPKEDPVPLPELPCEKS CCHHHHHCCCHHHHHHHCCCCEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC DAYFVLRDGAAGVFLAANTFPKSRETRAPLVEELYRFRDRLPEKLRYLADAPQQDPEGNK CCEEEEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH TMVRFSRKTKQQYVSSEKDGKATGWSAFYVDGKWVEGKK HHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCEECCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 3029379; 9097039; 9278503; 3032952; 8114910; 7779808 [H]