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The map label for this gene is luxQ [H]

Identifier: 89885900

GI number: 89885900

Start: 181175

End: 182695

Strand: Reverse

Name: luxQ [H]

Synonym: Rfer_4414

Alternate gene names: 89885900

Gene position: 182695-181175 (Counterclockwise)

Preceding gene: 89885902

Following gene: 89885899

Centisome position: 70.96

GC content: 47.6

Gene sequence:

>1521_bases
ATGGCACACCAATCAACCAGGATCAACATGAGTATTTTCACAGTCTCATTGCAGCTGTATTTGAAGGTTTTCCCGAGGCC
AAAATTCGAGGATCAGGACATGAAGCGGCTGTCGGGTGCTTATTCGGACATTAATGCTTTAACTATTATTTCTCTGCTCC
TGTATATGCTGGAGAACTTGTGGGTTAAGGCCAGTCCACCTGCCGCAGTGATGATGATCGTTTTCCTTGTTCTATTTCTG
ATCGCAGAAAGATGGGCAATATACAAGGGGTTTGCAAAGTTTTCAGTTTTGTCCTATGCATTCCTCATCGGGGCATTCTG
GTTAGGCTTGACTCACCTTGCAACCACCATCGTGCATACACAGACCATGATTTTGTGTTTAGGACTGGCCGCCGTGACCA
TAGCACTGGGGACGGGCTATGGTCTCGGGGTGCTATTTGCCTATGTCGGGTTGTTGTTGTGGGGGACTCTCGCTGAGCAA
GTAGCAGGAATGTCTCAAATCGCATACGCACAAACGATAAAGCACTTTGCCGTTGCTACTACCATCTTGACTGTGCTGTT
TGTCGTCTGCGCCATCATGCGGCGGCACTTGATAGAAGAAAGACAGTTAGCCTTGCGCGAACGTGACAGCGCCCGCGAAC
ATCTCGAACAAACCAACCAACTCCTGGCAAAACTTCTCTCAGAAAAGTCTGAACAACTGGATTTGACGGTAAACGAAACA
AATAAGGTCTTTGTTAAGCATGAACAGTTTTCCAATCTTGGAACCCTGGTCTCAGGCCTGTCGCATGAATTGGGTACGCC
GATAGGAAATGCAGTTTTGACCTCATCCAATATGATTGCTTGGAGCCATGAGATGCAAGAAAAATTGGGTCAAGATCCGC
AAAGAGAAAAACGTCTCAGTCAATACATGATGGAGGGGGCGCAAATCATCAGCAGAAACCTTGACCGGGCCAACACTCTT
GTGACCAATTTCAAACAAGTTAGTCTGGATCAATTGGGTAGCAATCGACTTACGGTCAACCTTGCAGACTGCATCAAACA
GTCTATTTTCGCGTTGAAGCCAACGCTTTCAAAATACGGTGTCAGAGTAAAATTGCGACTGGATGAGAGCATTAAGATGG
AGACATTTCCCTTCGCGATTGAGCAAATCGTTACCAATCTGGTGACAAACGCCGTGATACACGGACTTGAAAAGAAACCA
GATGGAATTGTGACGATAAGAACCTGGATCAAGTCAGGAACATCGGATGTCTGCATTGAAGTCGCAGACAACGGATGGGG
CATACACCATGAAGTAGTTACAAGAATCTTCGAGCCATTTTTCACCACTAAAAGAGGGCGCGGGGGGACGGGAATGGGCC
TTAGCATCGTCGCGCACCTTGCAACAGCGATCCTGGCCGGAGACATTGACGTGAACACGGGACCCGACGGATCCAAATTC
ATCGTCACGATTCCATTGAGTTCGCCCACTGTCGAAAACGATAGTTCCCCGCCAGACACAGTTCCGGGTTTTCTTCAATG
A

Upstream 100 bases:

>100_bases
GTTTCGCGTTCATTCAGCAGGACTTTGACGCAAATTGCTATTATCCCAAAAACCACAAAGCCTTGGCCGTCCATTCTGAA
GTGGACGCAGGACGGTGAAG

Downstream 100 bases:

>100_bases
ATAAGCAAGATAAAAAATGGAGAGTCCTTGTCGTTGACGACGATGCGGATGTTCTGACAAACACCGTCTACACCCTGATG
GATCTCAAGGTTTTAGGGCG

Product: periplasmic sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 506; Mature: 505

Protein sequence:

>506_residues
MAHQSTRINMSIFTVSLQLYLKVFPRPKFEDQDMKRLSGAYSDINALTIISLLLYMLENLWVKASPPAAVMMIVFLVLFL
IAERWAIYKGFAKFSVLSYAFLIGAFWLGLTHLATTIVHTQTMILCLGLAAVTIALGTGYGLGVLFAYVGLLLWGTLAEQ
VAGMSQIAYAQTIKHFAVATTILTVLFVVCAIMRRHLIEERQLALRERDSAREHLEQTNQLLAKLLSEKSEQLDLTVNET
NKVFVKHEQFSNLGTLVSGLSHELGTPIGNAVLTSSNMIAWSHEMQEKLGQDPQREKRLSQYMMEGAQIISRNLDRANTL
VTNFKQVSLDQLGSNRLTVNLADCIKQSIFALKPTLSKYGVRVKLRLDESIKMETFPFAIEQIVTNLVTNAVIHGLEKKP
DGIVTIRTWIKSGTSDVCIEVADNGWGIHHEVVTRIFEPFFTTKRGRGGTGMGLSIVAHLATAILAGDIDVNTGPDGSKF
IVTIPLSSPTVENDSSPPDTVPGFLQ

Sequences:

>Translated_506_residues
MAHQSTRINMSIFTVSLQLYLKVFPRPKFEDQDMKRLSGAYSDINALTIISLLLYMLENLWVKASPPAAVMMIVFLVLFL
IAERWAIYKGFAKFSVLSYAFLIGAFWLGLTHLATTIVHTQTMILCLGLAAVTIALGTGYGLGVLFAYVGLLLWGTLAEQ
VAGMSQIAYAQTIKHFAVATTILTVLFVVCAIMRRHLIEERQLALRERDSAREHLEQTNQLLAKLLSEKSEQLDLTVNET
NKVFVKHEQFSNLGTLVSGLSHELGTPIGNAVLTSSNMIAWSHEMQEKLGQDPQREKRLSQYMMEGAQIISRNLDRANTL
VTNFKQVSLDQLGSNRLTVNLADCIKQSIFALKPTLSKYGVRVKLRLDESIKMETFPFAIEQIVTNLVTNAVIHGLEKKP
DGIVTIRTWIKSGTSDVCIEVADNGWGIHHEVVTRIFEPFFTTKRGRGGTGMGLSIVAHLATAILAGDIDVNTGPDGSKF
IVTIPLSSPTVENDSSPPDTVPGFLQ
>Mature_505_residues
AHQSTRINMSIFTVSLQLYLKVFPRPKFEDQDMKRLSGAYSDINALTIISLLLYMLENLWVKASPPAAVMMIVFLVLFLI
AERWAIYKGFAKFSVLSYAFLIGAFWLGLTHLATTIVHTQTMILCLGLAAVTIALGTGYGLGVLFAYVGLLLWGTLAEQV
AGMSQIAYAQTIKHFAVATTILTVLFVVCAIMRRHLIEERQLALRERDSAREHLEQTNQLLAKLLSEKSEQLDLTVNETN
KVFVKHEQFSNLGTLVSGLSHELGTPIGNAVLTSSNMIAWSHEMQEKLGQDPQREKRLSQYMMEGAQIISRNLDRANTLV
TNFKQVSLDQLGSNRLTVNLADCIKQSIFALKPTLSKYGVRVKLRLDESIKMETFPFAIEQIVTNLVTNAVIHGLEKKPD
GIVTIRTWIKSGTSDVCIEVADNGWGIHHEVVTRIFEPFFTTKRGRGGTGMGLSIVAHLATAILAGDIDVNTGPDGSKFI
VTIPLSSPTVENDSSPPDTVPGFLQ

Specific function: At low cell density, in absence of autoinducer has a kinase activity, and autophosphorylates on a histidine residue. The phosphoryl group is then transferred to an aspartate residue in the response regulator domain. The phosphoryl group is transferred to

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=264, Percent_Identity=26.1363636363636, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI87081816, Length=116, Percent_Identity=34.4827586206897, Blast_Score=69, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR015387
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789
- ProDom:   PD142495 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF09308 LuxQ-periplasm; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 55953; Mature: 55822

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHQSTRINMSIFTVSLQLYLKVFPRPKFEDQDMKRLSGAYSDINALTIISLLLYMLENL
CCCCCCEEEEEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
WVKASPPAAVMMIVFLVLFLIAERWAIYKGFAKFSVLSYAFLIGAFWLGLTHLATTIVHT
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QTMILCLGLAAVTIALGTGYGLGVLFAYVGLLLWGTLAEQVAGMSQIAYAQTIKHFAVAT
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TILTVLFVVCAIMRRHLIEERQLALRERDSAREHLEQTNQLLAKLLSEKSEQLDLTVNET
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEECC
NKVFVKHEQFSNLGTLVSGLSHELGTPIGNAVLTSSNMIAWSHEMQEKLGQDPQREKRLS
CEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCEEHHHHHHHHHCCCHHHHHHHH
QYMMEGAQIISRNLDRANTLVTNFKQVSLDQLGSNRLTVNLADCIKQSIFALKPTLSKYG
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHCCCHHHCC
VRVKLRLDESIKMETFPFAIEQIVTNLVTNAVIHGLEKKPDGIVTIRTWIKSGTSDVCIE
EEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHCCCCCEEEE
VADNGWGIHHEVVTRIFEPFFTTKRGRGGTGMGLSIVAHLATAILAGDIDVNTGPDGSKF
ECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
IVTIPLSSPTVENDSSPPDTVPGFLQ
EEEEECCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AHQSTRINMSIFTVSLQLYLKVFPRPKFEDQDMKRLSGAYSDINALTIISLLLYMLENL
CCCCCEEEEEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
WVKASPPAAVMMIVFLVLFLIAERWAIYKGFAKFSVLSYAFLIGAFWLGLTHLATTIVHT
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QTMILCLGLAAVTIALGTGYGLGVLFAYVGLLLWGTLAEQVAGMSQIAYAQTIKHFAVAT
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TILTVLFVVCAIMRRHLIEERQLALRERDSAREHLEQTNQLLAKLLSEKSEQLDLTVNET
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEECC
NKVFVKHEQFSNLGTLVSGLSHELGTPIGNAVLTSSNMIAWSHEMQEKLGQDPQREKRLS
CEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCEEHHHHHHHHHCCCHHHHHHHH
QYMMEGAQIISRNLDRANTLVTNFKQVSLDQLGSNRLTVNLADCIKQSIFALKPTLSKYG
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHCCCHHHCC
VRVKLRLDESIKMETFPFAIEQIVTNLVTNAVIHGLEKKPDGIVTIRTWIKSGTSDVCIE
EEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHCCCCCEEEE
VADNGWGIHHEVVTRIFEPFFTTKRGRGGTGMGLSIVAHLATAILAGDIDVNTGPDGSKF
ECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
IVTIPLSSPTVENDSSPPDTVPGFLQ
EEEEECCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]