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The map label for this gene is dacF [H]

Identifier: 89898744

GI number: 89898744

Start: 1081706

End: 1083007

Strand: Direct

Name: dacF [H]

Synonym: CF0937

Alternate gene names: 89898744

Gene position: 1081706-1083007 (Clockwise)

Preceding gene: 89898742

Following gene: 89898745

Centisome position: 92.75

GC content: 41.94

Gene sequence:

>1302_bases
ATGACAAGAGTTTTTTTAATATCTGGACTGTTTAGTTTGCTTTTAGGATTATTCACGGTTCCTTCATATGGGGACATACC
TTTTCCTGAAACCCGAGGGAATGCTGCAGCTGTTATTCATGCCGACTCTGGCAAGATACTTTATGCTAAGGATATAGACA
AAAAAATCTATCCTGCGAGCATGACTAAGATCGCCACAGCCCTATTTATTTTAAAAAAGCATCCCGAAGTGTTGAATCGT
TTCATAACCGTAAAACAGGATGCGATTGCCTCTATTACTCCTCAGGCTAAAAAACAATCAGGATATCGCAGCCCCCCGCA
TTGGCTAGAAACTGACGGAGTGACAATACAGTTACAAAACAAAGAAGAAGTCTCGGGCTGGGACTTATTTCATGCGTTAT
TGATCTGTTCTGCTAACGATGCGGCAAATACTTTAGCCACAGCTTGCTCAGAATCTGTAGCAGAGTTTATGAAGCAATTA
AACACATTCCTTAGGGAATTAGGGTGCGAGCATACGCATTTTAACAATCCCCATGGTCTACATCATCCTAACCACTACAC
AACAGCGCGTGATTTAACACGCATTATGCGTGAGTGTTTAAAAGAGCCCTTATTCCGTCAGGTTATCCGAACAACCAACT
ATAAAATGGCGCCTACAAACCTTAGCCAAGAAAGAGTTCTAAATCTAACGAATAAGCTAATTCTTCCCGGATCTACTTAT
TACTATCCTCCTGCTTTAGGGGGAAAAACGGGAACGACAAAAGATGCGGGAAAAAACCTTATCTTTGCAGCGAAGAAGCA
TGGCCGGTCTATTATCACGATAGCTGCCGGTTATCCTGCCATGGGGGAACTGTATGAGGATGTGATTGCTTTATGCGAAG
GAGTCTTCAATGAACCTTTATTACGAAGATATTTAATTCCTCCGACAGAAAAATACACGCTGCGGTTGGGTCTTTTAGGG
AAAGTTTCTATTCCTTTACCTCAGGGAGTATATTACGATTTCTATGCTTCTGAGGAAGAAGAACATCAGACAATTTCCTT
TGTTCCTCACGCAACAAAGTTTCCCATTCACCGAGGAGATCTTCTGGGTCATTGGGTATTTCGTGGTCTTTCAGGAATGC
AAATCCGTGCCGAGCCCCTGTATGCCTCCGAGACTATCCAACCTTCCATTACTCAGAAGTTTCGCTTGTATGCCAAGAGA
GCTGTGACCTCTTACAGAACGTATATAGTCCTAACATTAATACTGCTTTATTACAGAAAAACTCGGATGCATAGGCATAA
ATCTTCTCGTTACTACCTATAA

Upstream 100 bases:

>100_bases
CTCCTACTTATGGTAATTCTTTATTTTAAGAGGCGTCAAGAGCTTTCTCTGGCGGTTGCGAAGATTTAACCCTAAGTAAC
AAGCTATCAAAATCCCTACA

Downstream 100 bases:

>100_bases
TACAACTCTTTCTTTTAGCTTATGGAAAACCTGATTTTCGCATAAACTACACGGGTACTATTGAGAATAAGGCTTATCAT
GCGCAAATATCTAGTTTGTA

Product: penicillin-binding protein 5

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MTRVFLISGLFSLLLGLFTVPSYGDIPFPETRGNAAAVIHADSGKILYAKDIDKKIYPASMTKIATALFILKKHPEVLNR
FITVKQDAIASITPQAKKQSGYRSPPHWLETDGVTIQLQNKEEVSGWDLFHALLICSANDAANTLATACSESVAEFMKQL
NTFLRELGCEHTHFNNPHGLHHPNHYTTARDLTRIMRECLKEPLFRQVIRTTNYKMAPTNLSQERVLNLTNKLILPGSTY
YYPPALGGKTGTTKDAGKNLIFAAKKHGRSIITIAAGYPAMGELYEDVIALCEGVFNEPLLRRYLIPPTEKYTLRLGLLG
KVSIPLPQGVYYDFYASEEEEHQTISFVPHATKFPIHRGDLLGHWVFRGLSGMQIRAEPLYASETIQPSITQKFRLYAKR
AVTSYRTYIVLTLILLYYRKTRMHRHKSSRYYL

Sequences:

>Translated_433_residues
MTRVFLISGLFSLLLGLFTVPSYGDIPFPETRGNAAAVIHADSGKILYAKDIDKKIYPASMTKIATALFILKKHPEVLNR
FITVKQDAIASITPQAKKQSGYRSPPHWLETDGVTIQLQNKEEVSGWDLFHALLICSANDAANTLATACSESVAEFMKQL
NTFLRELGCEHTHFNNPHGLHHPNHYTTARDLTRIMRECLKEPLFRQVIRTTNYKMAPTNLSQERVLNLTNKLILPGSTY
YYPPALGGKTGTTKDAGKNLIFAAKKHGRSIITIAAGYPAMGELYEDVIALCEGVFNEPLLRRYLIPPTEKYTLRLGLLG
KVSIPLPQGVYYDFYASEEEEHQTISFVPHATKFPIHRGDLLGHWVFRGLSGMQIRAEPLYASETIQPSITQKFRLYAKR
AVTSYRTYIVLTLILLYYRKTRMHRHKSSRYYL
>Mature_432_residues
TRVFLISGLFSLLLGLFTVPSYGDIPFPETRGNAAAVIHADSGKILYAKDIDKKIYPASMTKIATALFILKKHPEVLNRF
ITVKQDAIASITPQAKKQSGYRSPPHWLETDGVTIQLQNKEEVSGWDLFHALLICSANDAANTLATACSESVAEFMKQLN
TFLRELGCEHTHFNNPHGLHHPNHYTTARDLTRIMRECLKEPLFRQVIRTTNYKMAPTNLSQERVLNLTNKLILPGSTYY
YPPALGGKTGTTKDAGKNLIFAAKKHGRSIITIAAGYPAMGELYEDVIALCEGVFNEPLLRRYLIPPTEKYTLRLGLLGK
VSIPLPQGVYYDFYASEEEEHQTISFVPHATKFPIHRGDLLGHWVFRGLSGMQIRAEPLYASETIQPSITQKFRLYAKRA
VTSYRTYIVLTLILLYYRKTRMHRHKSSRYYL

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=381, Percent_Identity=26.246719160105, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87082022, Length=284, Percent_Identity=26.4084507042254, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1786851, Length=373, Percent_Identity=25.4691689008043, Blast_Score=72, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 49041; Mature: 48910

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRVFLISGLFSLLLGLFTVPSYGDIPFPETRGNAAAVIHADSGKILYAKDIDKKIYPAS
CCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCHH
MTKIATALFILKKHPEVLNRFITVKQDAIASITPQAKKQSGYRSPPHWLETDGVTIQLQN
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCEEEEECC
KEEVSGWDLFHALLICSANDAANTLATACSESVAEFMKQLNTFLRELGCEHTHFNNPHGL
CCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
HHPNHYTTARDLTRIMRECLKEPLFRQVIRTTNYKMAPTNLSQERVLNLTNKLILPGSTY
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHCEEECCCCE
YYPPALGGKTGTTKDAGKNLIFAAKKHGRSIITIAAGYPAMGELYEDVIALCEGVFNEPL
ECCCCCCCCCCCCHHHCCCEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCHH
LRRYLIPPTEKYTLRLGLLGKVSIPLPQGVYYDFYASEEEEHQTISFVPHATKFPIHRGD
HHHHCCCCCCCEEEEEEEEEEEECCCCCCEEEEEECCCCCCCCEEEECCCCCCCCCCCCH
LLGHWVFRGLSGMQIRAEPLYASETIQPSITQKFRLYAKRAVTSYRTYIVLTLILLYYRK
HHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TRMHRHKSSRYYL
HHHHHHCCCCCCC
>Mature Secondary Structure 
TRVFLISGLFSLLLGLFTVPSYGDIPFPETRGNAAAVIHADSGKILYAKDIDKKIYPAS
CEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCHH
MTKIATALFILKKHPEVLNRFITVKQDAIASITPQAKKQSGYRSPPHWLETDGVTIQLQN
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCEEEEECC
KEEVSGWDLFHALLICSANDAANTLATACSESVAEFMKQLNTFLRELGCEHTHFNNPHGL
CCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
HHPNHYTTARDLTRIMRECLKEPLFRQVIRTTNYKMAPTNLSQERVLNLTNKLILPGSTY
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHCEEECCCCE
YYPPALGGKTGTTKDAGKNLIFAAKKHGRSIITIAAGYPAMGELYEDVIALCEGVFNEPL
ECCCCCCCCCCCCHHHCCCEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCHH
LRRYLIPPTEKYTLRLGLLGKVSIPLPQGVYYDFYASEEEEHQTISFVPHATKFPIHRGD
HHHHCCCCCCCEEEEEEEEEEEECCCCCCEEEEEECCCCCCCCEEEECCCCCCCCCCCCH
LLGHWVFRGLSGMQIRAEPLYASETIQPSITQKFRLYAKRAVTSYRTYIVLTLILLYYRK
HHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TRMHRHKSSRYYL
HHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]