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The map label for this gene is ada [H]

Identifier: 89898669

GI number: 89898669

Start: 1012447

End: 1012974

Strand: Direct

Name: ada [H]

Synonym: CF0862

Alternate gene names: 89898669

Gene position: 1012447-1012974 (Clockwise)

Preceding gene: 89898668

Following gene: 89898673

Centisome position: 86.81

GC content: 41.48

Gene sequence:

>528_bases
ATGTCCGAGAATCTTTACCTAGTTTCCGACGATTCTAAATTTTCCCTATCGCAAGCCTGTTCTCAAGGCTTGCAGATAGC
TAAGTACCCGCCTCTCCAAGTCATAGTTCACTTCCAAAACAATACCGTCATAAAAACTCAGCTTTCTATAGCTCCTGTAT
TTTCCTGTTTATTTCTTGGTCCCGGCTCACATAAAGCTATGGAAGAAATTGTCCTACTATGTGCTAAGTATTCACAGAAA
ATTGCCATTCCGCAATCCTCTTATATCAATACATCCGTGTTGTCTGGACAGCAGGAAACAATTCTGAATAGCGTTGCGCA
AATTCCTTTCGGGCAGACGCGCACATATAAAGATATCGCCAGAGAAACCGATACGCATCCACGTACAGTAGGATCCGCGT
GTAAGAATAATCCCTTTCTTCTATTCTTCCCCTGTCATAGGGTAATAGGAAGTAACGGCGAACGTCATTACTGTGCTGGA
AAACAAATCCAAAATATTCTTCTGAACTTTGAGGAATCTTTAAGTTAG

Upstream 100 bases:

>100_bases
CCGCCGGAATTTATAAATGTGAGTGGTATCATCGCGGACGCCTGGTCTCAAAAACAAAGTTCGAGAGACTCAATAATGCT
GGATAATATAGTTTGTTTTC

Downstream 100 bases:

>100_bases
TCCTGCAGCTTAGGATCCAAAGCATCCCGAACACCGTCACCTATCAATGCTATAGCAATAAGCAATGCGGTAAGCATGAT
AGCTGGAGGCCAAAGAATCG

Product: methylated-DNA--protein-cysteine S-methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 175; Mature: 174

Protein sequence:

>175_residues
MSENLYLVSDDSKFSLSQACSQGLQIAKYPPLQVIVHFQNNTVIKTQLSIAPVFSCLFLGPGSHKAMEEIVLLCAKYSQK
IAIPQSSYINTSVLSGQQETILNSVAQIPFGQTRTYKDIARETDTHPRTVGSACKNNPFLLFFPCHRVIGSNGERHYCAG
KQIQNILLNFEESLS

Sequences:

>Translated_175_residues
MSENLYLVSDDSKFSLSQACSQGLQIAKYPPLQVIVHFQNNTVIKTQLSIAPVFSCLFLGPGSHKAMEEIVLLCAKYSQK
IAIPQSSYINTSVLSGQQETILNSVAQIPFGQTRTYKDIARETDTHPRTVGSACKNNPFLLFFPCHRVIGSNGERHYCAG
KQIQNILLNFEESLS
>Mature_174_residues
SENLYLVSDDSKFSLSQACSQGLQIAKYPPLQVIVHFQNNTVIKTQLSIAPVFSCLFLGPGSHKAMEEIVLLCAKYSQKI
AIPQSSYINTSVLSGQQETILNSVAQIPFGQTRTYKDIARETDTHPRTVGSACKNNPFLLFFPCHRVIGSNGERHYCAGK
QIQNILLNFEESLS

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1 [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 19413; Mature: 19282

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENLYLVSDDSKFSLSQACSQGLQIAKYPPLQVIVHFQNNTVIKTQLSIAPVFSCLFLG
CCCCEEEEECCCCCCHHHHHHHCCEECCCCCEEEEEEECCCEEEEEEHHHHHHHHHHHCC
PGSHKAMEEIVLLCAKYSQKIAIPQSSYINTSVLSGQQETILNSVAQIPFGQTRTYKDIA
CCCHHHHHHHHHHHHHHHCEECCCCCCCCCHHHHCCCHHHHHHHHHHCCCCCCCHHHHHH
RETDTHPRTVGSACKNNPFLLFFPCHRVIGSNGERHYCAGKQIQNILLNFEESLS
HHCCCCCHHHHHHHCCCCEEEEEECHHHHCCCCCEEEEHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SENLYLVSDDSKFSLSQACSQGLQIAKYPPLQVIVHFQNNTVIKTQLSIAPVFSCLFLG
CCCEEEEECCCCCCHHHHHHHCCEECCCCCEEEEEEECCCEEEEEEHHHHHHHHHHHCC
PGSHKAMEEIVLLCAKYSQKIAIPQSSYINTSVLSGQQETILNSVAQIPFGQTRTYKDIA
CCCHHHHHHHHHHHHHHHCEECCCCCCCCCHHHHCCCHHHHHHHHHHCCCCCCCHHHHHH
RETDTHPRTVGSACKNNPFLLFFPCHRVIGSNGERHYCAGKQIQNILLNFEESLS
HHCCCCCHHHHHHHCCCCEEEEEECHHHHCCCCCEEEEHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA