Click here to switch to the map view.

The map label for this gene is truA

Identifier: 89898653

GI number: 89898653

Start: 995516

End: 996322

Strand: Direct

Name: truA

Synonym: CF0846

Alternate gene names: 89898653

Gene position: 995516-996322 (Clockwise)

Preceding gene: 89898652

Following gene: 89898655

Centisome position: 85.36

GC content: 41.51

Gene sequence:

>807_bases
ATGAAACGAGTTGTTTTACTCTTAGCTTATCAGGGGACTGCCTATGCCGGTTGGCAAAGACAACCTAACGACCTCTCCAT
TCAAGAAGTTATAGAAAGCGCCTTAGCAAGAGTTTTAGGGAAGCCTATTGTTGTTTCCTCTTCAGGCCGTACCGACTCTG
GAGTTCATGCTTATGGTCAGGTAGCGCATTTTTCACAACCCGACCATCCGTTATTTTCTCAACCTCAGAGCATAAAAAAA
ATGCTCAATGCCATTTTACCTAAGGATATTGTTATCCGCGATGTTGTGCTTAGCGATGTAAACTTCCACGCTCGCTTCTC
AGCTATTGCTAAGGAATACCGTTACTCGCTAACAAGGTCTCCTAAACCTCTTCCTTGGCAACGGTATTTTGCCTATTACC
CACGACACTCTATAAAAATAGACCTTATGCAAAAAGGGGCAAAGTATCTATTAGGCACACATGATTTTGCCTCTTTTGCC
AATCATGGACGTGATTATACCTCGACAGAGAGAACTTTATTCAAATTAGACATTATAGATAATGAGGAAATAGTCACCAT
CATATGCAAAGGCAACGGGTTTTTGTATAAAATGGTAAGAAATATCGTAGGATCTCTTTTAGACATTAGCAAAGAGAAAT
ACCCTCCTGAATATATCCAAGAAATCCTAGCACAGAAAAGTCGCAGGAAGGGACCGCCAGCGGCTCCTAGCCACGCGTTG
TCTTTGCACCACGTATGTTATCCTAAACCCTACAACTGGTTTTGTACTCCAGAGTGCGACATTAACTCTTTAAAAGAAGA
AAAATAG

Upstream 100 bases:

>100_bases
CAGCGGAGCTTCTAGGCATTCCTGTATCTTTAGTATCTAATAAACATCCACAAATAAAAATTACTTATCCTGAAGATTTA
ACCATCGCTCATGCTCTGCT

Downstream 100 bases:

>100_bases
TGAAAGTCTTTTTCATAAAAATCTTCATGGCTATTGTGAGACAATGGCAAAGTCGAATTTACAGCAACTAACGTTGCGGG
AATTCCTGAAAGAGCGCGCA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MKRVVLLLAYQGTAYAGWQRQPNDLSIQEVIESALARVLGKPIVVSSSGRTDSGVHAYGQVAHFSQPDHPLFSQPQSIKK
MLNAILPKDIVIRDVVLSDVNFHARFSAIAKEYRYSLTRSPKPLPWQRYFAYYPRHSIKIDLMQKGAKYLLGTHDFASFA
NHGRDYTSTERTLFKLDIIDNEEIVTIICKGNGFLYKMVRNIVGSLLDISKEKYPPEYIQEILAQKSRRKGPPAAPSHAL
SLHHVCYPKPYNWFCTPECDINSLKEEK

Sequences:

>Translated_268_residues
MKRVVLLLAYQGTAYAGWQRQPNDLSIQEVIESALARVLGKPIVVSSSGRTDSGVHAYGQVAHFSQPDHPLFSQPQSIKK
MLNAILPKDIVIRDVVLSDVNFHARFSAIAKEYRYSLTRSPKPLPWQRYFAYYPRHSIKIDLMQKGAKYLLGTHDFASFA
NHGRDYTSTERTLFKLDIIDNEEIVTIICKGNGFLYKMVRNIVGSLLDISKEKYPPEYIQEILAQKSRRKGPPAAPSHAL
SLHHVCYPKPYNWFCTPECDINSLKEEK
>Mature_268_residues
MKRVVLLLAYQGTAYAGWQRQPNDLSIQEVIESALARVLGKPIVVSSSGRTDSGVHAYGQVAHFSQPDHPLFSQPQSIKK
MLNAILPKDIVIRDVVLSDVNFHARFSAIAKEYRYSLTRSPKPLPWQRYFAYYPRHSIKIDLMQKGAKYLLGTHDFASFA
NHGRDYTSTERTLFKLDIIDNEEIVTIICKGNGFLYKMVRNIVGSLLDISKEKYPPEYIQEILAQKSRRKGPPAAPSHAL
SLHHVCYPKPYNWFCTPECDINSLKEEK

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=286, Percent_Identity=27.6223776223776, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI199559785, Length=280, Percent_Identity=25.7142857142857, Blast_Score=72, Evalue=4e-13,
Organism=Escherichia coli, GI1788657, Length=253, Percent_Identity=35.9683794466403, Blast_Score=149, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI71985046, Length=281, Percent_Identity=24.1992882562278, Blast_Score=77, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_CHLFF (Q253C0)

Other databases:

- EMBL:   AP006861
- RefSeq:   YP_515763.1
- ProteinModelPortal:   Q253C0
- SMR:   Q253C0
- STRING:   Q253C0
- GeneID:   3959314
- GenomeReviews:   AP006861_GR
- KEGG:   cfe:CF0846
- eggNOG:   COG0101
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- ProtClustDB:   PRK00021
- BioCyc:   CFEL264202:CF0846-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 30553; Mature: 30553

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRVVLLLAYQGTAYAGWQRQPNDLSIQEVIESALARVLGKPIVVSSSGRTDSGVHAYGQ
CCEEEEEEEECCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHH
VAHFSQPDHPLFSQPQSIKKMLNAILPKDIVIRDVVLSDVNFHARFSAIAKEYRYSLTRS
HHCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
PKPLPWQRYFAYYPRHSIKIDLMQKGAKYLLGTHDFASFANHGRDYTSTERTLFKLDIID
CCCCCHHHHHHHCCCCCEEEHHHHCCHHHHCCCHHHHHHHHCCCCCCCCCCEEEEEEEEC
NEEIVTIICKGNGFLYKMVRNIVGSLLDISKEKYPPEYIQEILAQKSRRKGPPAAPSHAL
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHE
SLHHVCYPKPYNWFCTPECDINSLKEEK
EEHHEECCCCCCEEECCCCCCCCCCCCC
>Mature Secondary Structure
MKRVVLLLAYQGTAYAGWQRQPNDLSIQEVIESALARVLGKPIVVSSSGRTDSGVHAYGQ
CCEEEEEEEECCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHH
VAHFSQPDHPLFSQPQSIKKMLNAILPKDIVIRDVVLSDVNFHARFSAIAKEYRYSLTRS
HHCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
PKPLPWQRYFAYYPRHSIKIDLMQKGAKYLLGTHDFASFANHGRDYTSTERTLFKLDIID
CCCCCHHHHHHHCCCCCEEEHHHHCCHHHHCCCHHHHHHHHCCCCCCCCCCEEEEEEEEC
NEEIVTIICKGNGFLYKMVRNIVGSLLDISKEKYPPEYIQEILAQKSRRKGPPAAPSHAL
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHE
SLHHVCYPKPYNWFCTPECDINSLKEEK
EEHHEECCCCCCEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA