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The map label for this gene is ispD

Identifier: 89898652

GI number: 89898652

Start: 994881

End: 995519

Strand: Direct

Name: ispD

Synonym: CF0845

Alternate gene names: 89898652

Gene position: 994881-995519 (Clockwise)

Preceding gene: 89898651

Following gene: 89898653

Centisome position: 85.31

GC content: 39.75

Gene sequence:

>639_bases
ATGAATCCTAAATGTTCTTTGATCTTGCTTAGTGGGGGAAAAGGAGAGCGCTTCGGAGCAAATCAACCTAAACAATACTT
ACCTTTTCAAGGTCAGCCTCTTATCTTACATGCTTTAAACTCAGCTCTGCTCATTCCTGAAATCAATGAAATCATTGTAG
TGTGTGATGCTAGCTATGAGCATATTTTTGAAGGTTATTCTGTAAAATTTGCTCCAGCTGGAATGCGTCGTCAGGATTCT
GTTTTTTCGGGATTACACCACGTTACCAATCCTTGGGTCTTAGTACACGACGGTGTACGACCTTTTATCTATCCTGATGA
AGTTTCTGAACTTATTGTTATTGCACAACAAACAGGGGCAGCAACACTCGTATCTAATGTCCCCTACACTATAAAACAAC
GCAATCCCGTAAAAACACTAGATCGCGATTCTTTATCTATTGTTCATACACCTCAATGCATCAAGACAGAGATCCTTTTG
GAAGGTCTTGAGCTAGCCGATAGAGAACAGATCACTCTTGTTGATGATACCCAAGCAGCGGAGCTTCTAGGCATTCCTGT
ATCTTTAGTATCTAATAAACATCCACAAATAAAAATTACTTATCCTGAAGATTTAACCATCGCTCATGCTCTGCTATGA

Upstream 100 bases:

>100_bases
TCTGGTGGGAAAAGACAATTGTATGTGAATCGTGGGCTGGGGGGATTGAAAAGATTGCGTTTTTGTTCTCCCCCAGAAAT
TTGTTGTGTTCGGTGTGTAT

Downstream 100 bases:

>100_bases
AACGAGTTGTTTTACTCTTAGCTTATCAGGGGACTGCCTATGCCGGTTGGCAAAGACAACCTAACGACCTCTCCATTCAA
GAAGTTATAGAAAGCGCCTT

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MNPKCSLILLSGGKGERFGANQPKQYLPFQGQPLILHALNSALLIPEINEIIVVCDASYEHIFEGYSVKFAPAGMRRQDS
VFSGLHHVTNPWVLVHDGVRPFIYPDEVSELIVIAQQTGAATLVSNVPYTIKQRNPVKTLDRDSLSIVHTPQCIKTEILL
EGLELADREQITLVDDTQAAELLGIPVSLVSNKHPQIKITYPEDLTIAHALL

Sequences:

>Translated_212_residues
MNPKCSLILLSGGKGERFGANQPKQYLPFQGQPLILHALNSALLIPEINEIIVVCDASYEHIFEGYSVKFAPAGMRRQDS
VFSGLHHVTNPWVLVHDGVRPFIYPDEVSELIVIAQQTGAATLVSNVPYTIKQRNPVKTLDRDSLSIVHTPQCIKTEILL
EGLELADREQITLVDDTQAAELLGIPVSLVSNKHPQIKITYPEDLTIAHALL
>Mature_212_residues
MNPKCSLILLSGGKGERFGANQPKQYLPFQGQPLILHALNSALLIPEINEIIVVCDASYEHIFEGYSVKFAPAGMRRQDS
VFSGLHHVTNPWVLVHDGVRPFIYPDEVSELIVIAQQTGAATLVSNVPYTIKQRNPVKTLDRDSLSIVHTPQCIKTEILL
EGLELADREQITLVDDTQAAELLGIPVSLVSNKHPQIKITYPEDLTIAHALL

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Escherichia coli, GI1789104, Length=211, Percent_Identity=30.8056872037915, Blast_Score=96, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_CHLFF (Q253C1)

Other databases:

- EMBL:   AP006861
- RefSeq:   YP_515762.1
- ProteinModelPortal:   Q253C1
- SMR:   Q253C1
- STRING:   Q253C1
- GeneID:   3959227
- GenomeReviews:   AP006861_GR
- KEGG:   cfe:CF0845
- eggNOG:   COG1211
- HOGENOM:   HBG672839
- OMA:   NTIPREN
- ProtClustDB:   PRK00155
- BioCyc:   CFEL264202:CF0845-MONOMER
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 23420; Mature: 23420

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPKCSLILLSGGKGERFGANQPKQYLPFQGQPLILHALNSALLIPEINEIIVVCDASYE
CCCCEEEEEEECCCCCCCCCCCCHHCCCCCCCEEEEEECCCEEECCCCCEEEEEECCCHH
HIFEGYSVKFAPAGMRRQDSVFSGLHHVTNPWVLVHDGVRPFIYPDEVSELIVIAQQTGA
HHHCCCEEEEECCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCEEEEEEECCCC
ATLVSNVPYTIKQRNPVKTLDRDSLSIVHTPQCIKTEILLEGLELADREQITLVDDTQAA
EEEECCCCEEEECCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCCCCEEEEEECCCCH
ELLGIPVSLVSNKHPQIKITYPEDLTIAHALL
HHHCCCHHHHCCCCCEEEEECCCCCCCHHHCC
>Mature Secondary Structure
MNPKCSLILLSGGKGERFGANQPKQYLPFQGQPLILHALNSALLIPEINEIIVVCDASYE
CCCCEEEEEEECCCCCCCCCCCCHHCCCCCCCEEEEEECCCEEECCCCCEEEEEECCCHH
HIFEGYSVKFAPAGMRRQDSVFSGLHHVTNPWVLVHDGVRPFIYPDEVSELIVIAQQTGA
HHHCCCEEEEECCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCEEEEEEECCCC
ATLVSNVPYTIKQRNPVKTLDRDSLSIVHTPQCIKTEILLEGLELADREQITLVDDTQAA
EEEECCCCEEEECCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCCCCEEEEEECCCCH
ELLGIPVSLVSNKHPQIKITYPEDLTIAHALL
HHHCCCHHHHCCCCCEEEEECCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA