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The map label for this gene is vacB [H]

Identifier: 89898573

GI number: 89898573

Start: 911361

End: 913391

Strand: Direct

Name: vacB [H]

Synonym: CF0766

Alternate gene names: 89898573

Gene position: 911361-913391 (Clockwise)

Preceding gene: 89898572

Following gene: 89898574

Centisome position: 78.15

GC content: 40.82

Gene sequence:

>2031_bases
GTGAGAAAAATAAAAAAAAGAAGACCTAAAGTCTTTAAACGAAGCACTAAGCAAATCCTACTCTCCGGAATCCTATTTGT
GCACGCAAAAAAAGGTTTTGGATTTGTTACTCCTGACCATCCAGAGGAATACCCTTTTGACATTTTCATTCCTGCGAGAG
ACCTTAAAGGTGCTTTAGATGGAGATCATGTTATCGTCTCCCTTTTCCCCCATTCTAAAGAAGGAGAAAAAAGAAAAGGA
GTTATTCATCAAGTTGTATCAAGAGGAAAAACTGTTCTTGTAGGAACTATAACCTCAATTATTGACGAAACTACTGCTCT
TGTTTGTGTGAATGTTTTAGGCCCAGATGTCCCTGTAAAAGCTAAACTTATCCCTAAACGCTCTTATAAAATTGGAGATC
GTTTACTTCTCAGCACGCCCGAATGGAAAGAAAAGCCAGAATCTAAAGAACCTCCACCTCTACAAATGCTTGAATTTATT
GGGAATATCTCCAATGCCAAGTCTGATTTCCCCTGTATAAAAGCGGAATTTGCAATCGAAGAAGACTTCCCAGATGCTGT
TATCGAAGAAACCAACCACTTCTCCCAAAAGCATATCGCCCAAGCACTAAGATCACGCAAGGACCTCCGTGACCTCCTCT
GCTTCACGATTGACTCCATAACAGCAAAAGATTTCGATGATGCTGTGTCATTAACCTATGACCATAATGACAACTACATT
CTTGGCGTCCATATTGCAGACGTTTCCCACTACGTAACCCCTCATTCTGCTCTAGATCAAGAAGCAAGTAAAAGATGTAA
CTCAATTTACTTTCCTGGGAAAGTGATCCCTATGTTACCTCCAGCACTTTCTGACAATCTCTGTAGCTTAAAACCTAATG
TAGATCGACTTGCCGTTTCCGTGTTCATGACATTTACAAAATCAGGTCATCTATCCAATTACGAAATATTCCGCAGTGTG
ATTCGTAGTAAATACCGCATGACCTACGACGAAGTGGATGAAATTGTAGAAAATAAACAACCCCATCCCATTTCAAAAAC
TCTCCTTGCCATGGCAGAGCTAAGCGAAAAATTCGCAGATATCAGAGAAAAGCGCGGCTGCATACGCCTGATTCTTCCCT
CATTTACCATGGCACTAGACAATCTACAAGAACCCACCGCTCTTATAGAAACCCGACAAACCTTGTCGCATAAACTTATC
GAAGAGTTTATGCTCAAAGCTAACGAAGTCATTGCCTACCATATTTCCCATCAAGGGTTCTCACTGCCATTCAGGATTCA
TGAATCTCCAAATGATGAAAGTTTACTCTCCTTCCAAGAAATGGCCAAAGCTATGGGGTTTGATATAATCATGACCCCAA
CTCAAGAACCCGATTATCAATACTTGCTTCAAGAAAGCTCTTCAGGACACCCTTTAGAGCCAATTCTACATTCACAATTT
GTCCGAAGCATGAAAACCGCCTCGTACTCCACAGAAAACAAAGGCCATTACGGACTGAAACTCGACTTCTATACTCATTT
TACAAGCCCTATTCGCCGGTATATCGATCTTGTTGTTCACAGACTGCTTTTCCACCCTATGTCCATAGAAGAAACACGCT
TAGAACACATTGTAAGAGCCTGCTCTTCCCAAGAGCGTATAGCCGCAAAAGCCGAATTTGCTTTTGAAAATCTGAAAAAA
ACACGGTTCTTAAATAAATTCTTACAAGAACAGCCAGAAACAATTTATAAAGCCTACATTATCACTACAACTCCCGAAGG
AATTTCGTTTACTGTTCCTGAGTTTTGCCAAGAAGGATGCATTCCTACAGCTCAACTGCCTAAAGAATACGCTCTGACAA
AGAAAACTTCTCCTGACGATCTCCCAAGCAAACTCCGCCCAGGAGCAACGATAGAAGTCAAACTCTCCGAAGTCAATCTC
CTCAATCAGGCAATTACCTGGTCCTTGGTAACAAAAAAAATAAGGACACCCAAAAAACAACCCACAAAGGAACAAACACC
TAAAACTCGAAGAAAAAGGAAAACAGAATAA

Upstream 100 bases:

>100_bases
CTTTTTAACTCTATCCAGAAAAATATCTTTGCATTAAAAACACCCAACAAAGGCTAGGTAAACCTATGAAAATCATAAGT
CCAGACATGGAGCAAAGTTG

Downstream 100 bases:

>100_bases
TGCTTCCTGAAAGCTTCTTTCTAAACGATGATGTTCTCTATCTAGCCAAAGAATTACTGGGACATAGTCTCGTCACTCAA
ATAGAAGGAAAAACAACCTC

Product: ribonuclease R

Products: NA

Alternate protein names: RNase R; VacB protein homolog [H]

Number of amino acids: Translated: 676; Mature: 676

Protein sequence:

>676_residues
MRKIKKRRPKVFKRSTKQILLSGILFVHAKKGFGFVTPDHPEEYPFDIFIPARDLKGALDGDHVIVSLFPHSKEGEKRKG
VIHQVVSRGKTVLVGTITSIIDETTALVCVNVLGPDVPVKAKLIPKRSYKIGDRLLLSTPEWKEKPESKEPPPLQMLEFI
GNISNAKSDFPCIKAEFAIEEDFPDAVIEETNHFSQKHIAQALRSRKDLRDLLCFTIDSITAKDFDDAVSLTYDHNDNYI
LGVHIADVSHYVTPHSALDQEASKRCNSIYFPGKVIPMLPPALSDNLCSLKPNVDRLAVSVFMTFTKSGHLSNYEIFRSV
IRSKYRMTYDEVDEIVENKQPHPISKTLLAMAELSEKFADIREKRGCIRLILPSFTMALDNLQEPTALIETRQTLSHKLI
EEFMLKANEVIAYHISHQGFSLPFRIHESPNDESLLSFQEMAKAMGFDIIMTPTQEPDYQYLLQESSSGHPLEPILHSQF
VRSMKTASYSTENKGHYGLKLDFYTHFTSPIRRYIDLVVHRLLFHPMSIEETRLEHIVRACSSQERIAAKAEFAFENLKK
TRFLNKFLQEQPETIYKAYIITTTPEGISFTVPEFCQEGCIPTAQLPKEYALTKKTSPDDLPSKLRPGATIEVKLSEVNL
LNQAITWSLVTKKIRTPKKQPTKEQTPKTRRKRKTE

Sequences:

>Translated_676_residues
MRKIKKRRPKVFKRSTKQILLSGILFVHAKKGFGFVTPDHPEEYPFDIFIPARDLKGALDGDHVIVSLFPHSKEGEKRKG
VIHQVVSRGKTVLVGTITSIIDETTALVCVNVLGPDVPVKAKLIPKRSYKIGDRLLLSTPEWKEKPESKEPPPLQMLEFI
GNISNAKSDFPCIKAEFAIEEDFPDAVIEETNHFSQKHIAQALRSRKDLRDLLCFTIDSITAKDFDDAVSLTYDHNDNYI
LGVHIADVSHYVTPHSALDQEASKRCNSIYFPGKVIPMLPPALSDNLCSLKPNVDRLAVSVFMTFTKSGHLSNYEIFRSV
IRSKYRMTYDEVDEIVENKQPHPISKTLLAMAELSEKFADIREKRGCIRLILPSFTMALDNLQEPTALIETRQTLSHKLI
EEFMLKANEVIAYHISHQGFSLPFRIHESPNDESLLSFQEMAKAMGFDIIMTPTQEPDYQYLLQESSSGHPLEPILHSQF
VRSMKTASYSTENKGHYGLKLDFYTHFTSPIRRYIDLVVHRLLFHPMSIEETRLEHIVRACSSQERIAAKAEFAFENLKK
TRFLNKFLQEQPETIYKAYIITTTPEGISFTVPEFCQEGCIPTAQLPKEYALTKKTSPDDLPSKLRPGATIEVKLSEVNL
LNQAITWSLVTKKIRTPKKQPTKEQTPKTRRKRKTE
>Mature_676_residues
MRKIKKRRPKVFKRSTKQILLSGILFVHAKKGFGFVTPDHPEEYPFDIFIPARDLKGALDGDHVIVSLFPHSKEGEKRKG
VIHQVVSRGKTVLVGTITSIIDETTALVCVNVLGPDVPVKAKLIPKRSYKIGDRLLLSTPEWKEKPESKEPPPLQMLEFI
GNISNAKSDFPCIKAEFAIEEDFPDAVIEETNHFSQKHIAQALRSRKDLRDLLCFTIDSITAKDFDDAVSLTYDHNDNYI
LGVHIADVSHYVTPHSALDQEASKRCNSIYFPGKVIPMLPPALSDNLCSLKPNVDRLAVSVFMTFTKSGHLSNYEIFRSV
IRSKYRMTYDEVDEIVENKQPHPISKTLLAMAELSEKFADIREKRGCIRLILPSFTMALDNLQEPTALIETRQTLSHKLI
EEFMLKANEVIAYHISHQGFSLPFRIHESPNDESLLSFQEMAKAMGFDIIMTPTQEPDYQYLLQESSSGHPLEPILHSQF
VRSMKTASYSTENKGHYGLKLDFYTHFTSPIRRYIDLVVHRLLFHPMSIEETRLEHIVRACSSQERIAAKAEFAFENLKK
TRFLNKFLQEQPETIYKAYIITTTPEGISFTVPEFCQEGCIPTAQLPKEYALTKKTSPDDLPSKLRPGATIEVKLSEVNL
LNQAITWSLVTKKIRTPKKQPTKEQTPKTRRKRKTE

Specific function: 3'-5'exoribonuclease that participates in an essential cell function. Acts nonspecifically on poly(A), poly(U) and ribosomal RNAs [H]

COG id: COG0557

COG function: function code K; Exoribonuclease R

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI190014623, Length=559, Percent_Identity=27.7280858676207, Blast_Score=176, Evalue=9e-44,
Organism=Homo sapiens, GI190014625, Length=559, Percent_Identity=27.7280858676207, Blast_Score=175, Evalue=1e-43,
Organism=Homo sapiens, GI219521928, Length=475, Percent_Identity=30.7368421052632, Blast_Score=173, Evalue=6e-43,
Organism=Homo sapiens, GI19115966, Length=479, Percent_Identity=31.1064718162839, Blast_Score=172, Evalue=6e-43,
Organism=Homo sapiens, GI134288890, Length=409, Percent_Identity=31.0513447432763, Blast_Score=167, Evalue=4e-41,
Organism=Homo sapiens, GI156105695, Length=369, Percent_Identity=26.2872628726287, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI156105693, Length=369, Percent_Identity=26.2872628726287, Blast_Score=103, Evalue=5e-22,
Organism=Escherichia coli, GI87082383, Length=668, Percent_Identity=28.7425149700599, Blast_Score=260, Evalue=2e-70,
Organism=Escherichia coli, GI1787542, Length=569, Percent_Identity=26.713532513181, Blast_Score=153, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI212645896, Length=410, Percent_Identity=29.7560975609756, Blast_Score=172, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI17553506, Length=363, Percent_Identity=32.2314049586777, Blast_Score=166, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6324552, Length=570, Percent_Identity=29.1228070175439, Blast_Score=192, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6320499, Length=426, Percent_Identity=24.4131455399061, Blast_Score=98, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6323943, Length=433, Percent_Identity=25.1732101616628, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24649634, Length=412, Percent_Identity=30.3398058252427, Blast_Score=169, Evalue=7e-42,
Organism=Drosophila melanogaster, GI24654592, Length=351, Percent_Identity=33.6182336182336, Blast_Score=167, Evalue=3e-41,
Organism=Drosophila melanogaster, GI19922976, Length=351, Percent_Identity=33.6182336182336, Blast_Score=167, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24654597, Length=351, Percent_Identity=33.6182336182336, Blast_Score=167, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011129
- InterPro:   IPR022967
- InterPro:   IPR013223
- InterPro:   IPR001900
- InterPro:   IPR022966
- InterPro:   IPR004476
- InterPro:   IPR011805 [H]

Pfam domain/function: PF08206 OB_RNB; PF00773 RNB [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 77095; Mature: 77095

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: PS01175 RIBONUCLEASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKIKKRRPKVFKRSTKQILLSGILFVHAKKGFGFVTPDHPEEYPFDIFIPARDLKGALD
CCCHHHHCCHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCEEEEEECHHHCCCCC
GDHVIVSLFPHSKEGEKRKGVIHQVVSRGKTVLVGTITSIIDETTALVCVNVLGPDVPVK
CCEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHEEHHHCCCCCCCE
AKLIPKRSYKIGDRLLLSTPEWKEKPESKEPPPLQMLEFIGNISNAKSDFPCIKAEFAIE
EEECCCCCCCCCCEEEECCCCHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEEEC
EDFPDAVIEETNHFSQKHIAQALRSRKDLRDLLCFTIDSITAKDFDDAVSLTYDHNDNYI
CCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCEE
LGVHIADVSHYVTPHSALDQEASKRCNSIYFPGKVIPMLPPALSDNLCSLKPNVDRLAVS
EEEEEECHHHHCCCHHHHHHHHHHHHCEECCCCEECCCCCCCCCCCCCCCCCCHHHHHHH
VFMTFTKSGHLSNYEIFRSVIRSKYRMTYDEVDEIVENKQPHPISKTLLAMAELSEKFAD
HHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
IREKRGCIRLILPSFTMALDNLQEPTALIETRQTLSHKLIEEFMLKANEVIAYHISHQGF
HHHHCCEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCC
SLPFRIHESPNDESLLSFQEMAKAMGFDIIMTPTQEPDYQYLLQESSSGHPLEPILHSQF
CCCEEECCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHCCCCCCCCHHHHHHHH
VRSMKTASYSTENKGHYGLKLDFYTHFTSPIRRYIDLVVHRLLFHPMSIEETRLEHIVRA
HHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
CSSQERIAAKAEFAFENLKKTRFLNKFLQEQPETIYKAYIITTTPEGISFTVPEFCQEGC
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCEECCHHHHHCCC
IPTAQLPKEYALTKKTSPDDLPSKLRPGATIEVKLSEVNLLNQAITWSLVTKKIRTPKKQ
CCCHHCCHHHHHCCCCCCCCCHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCC
PTKEQTPKTRRKRKTE
CCCCCCCHHHHHCCCC
>Mature Secondary Structure
MRKIKKRRPKVFKRSTKQILLSGILFVHAKKGFGFVTPDHPEEYPFDIFIPARDLKGALD
CCCHHHHCCHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCEEEEEECHHHCCCCC
GDHVIVSLFPHSKEGEKRKGVIHQVVSRGKTVLVGTITSIIDETTALVCVNVLGPDVPVK
CCEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHEEHHHCCCCCCCE
AKLIPKRSYKIGDRLLLSTPEWKEKPESKEPPPLQMLEFIGNISNAKSDFPCIKAEFAIE
EEECCCCCCCCCCEEEECCCCHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEEEC
EDFPDAVIEETNHFSQKHIAQALRSRKDLRDLLCFTIDSITAKDFDDAVSLTYDHNDNYI
CCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCEE
LGVHIADVSHYVTPHSALDQEASKRCNSIYFPGKVIPMLPPALSDNLCSLKPNVDRLAVS
EEEEEECHHHHCCCHHHHHHHHHHHHCEECCCCEECCCCCCCCCCCCCCCCCCHHHHHHH
VFMTFTKSGHLSNYEIFRSVIRSKYRMTYDEVDEIVENKQPHPISKTLLAMAELSEKFAD
HHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
IREKRGCIRLILPSFTMALDNLQEPTALIETRQTLSHKLIEEFMLKANEVIAYHISHQGF
HHHHCCEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCC
SLPFRIHESPNDESLLSFQEMAKAMGFDIIMTPTQEPDYQYLLQESSSGHPLEPILHSQF
CCCEEECCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHCCCCCCCCHHHHHHHH
VRSMKTASYSTENKGHYGLKLDFYTHFTSPIRRYIDLVVHRLLFHPMSIEETRLEHIVRA
HHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
CSSQERIAAKAEFAFENLKKTRFLNKFLQEQPETIYKAYIITTTPEGISFTVPEFCQEGC
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCEECCHHHHHCCC
IPTAQLPKEYALTKKTSPDDLPSKLRPGATIEVKLSEVNLLNQAITWSLVTKKIRTPKKQ
CCCHHCCHHHHHCCCCCCCCCHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCC
PTKEQTPKTRRKRKTE
CCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9784136 [H]