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The map label for this gene is gcpE

Identifier: 89898391

GI number: 89898391

Start: 685815

End: 687635

Strand: Direct

Name: gcpE

Synonym: CF0584

Alternate gene names: 89898391

Gene position: 685815-687635 (Clockwise)

Preceding gene: 89898390

Following gene: 89898392

Centisome position: 58.81

GC content: 40.31

Gene sequence:

>1821_bases
ATGATTATTTCTCCCTTTAAAAAACAAACTGCTAGACGTTATACGCACTCAGTTAAAATTGGGAGTCTTTTTGTAGGCAG
TGAACATTCAATAAAAATGCAATCTATGACAACAACACCGACCTCGGATGTTGATGCCACAGTTGCTCAGATTTGCTCCT
TAGTAGAAGCTAAATGTGATATTGCTCGTGTTACTGTACAGGGAGTTAAGGAAGCTCAGGCATGTGAGCATATAAAAGAA
CGCCTGCTAACTATGGGTCTTGATATTCCTCTTGTTGCGGATATCCATTTCTTTCCTCAAGCAGCGATGCACGTTGCAGA
TTTCGTAGATAAAGTACGTATCAATCCTGGGAACTTTGTTGACAAGCGCAATATGTTCTCAGGAAAAACTTATACGGATA
AAAACTATGCTGATAGTCTTCTTCGATTAGAAGAAAAATTCTCTCCTCTTGTAGAAAAGTGCAAACGTCTAGGAAAAGCG
ATGAGAATCGGCGTGAATCATGGTTCTCTTTCTGAACGCATTATGCAACGTTATGGAGACACCATTGAGGGCATGGTAGT
TTCTGCTCTTGAGTATATCAAAGTATGTGAAAATCTTGGCTATCGCGATGTTGTTTTTTCGATGAAATCGAGTAATCCCA
AGGTTATGGTTGCAGCTTATCGCCAACTTGCAAAAGATCTTGATGCTCGGGGCTGGCATTATCCCCTACACCTAGGTGTT
ACAGAAGCTGGAATGGGAATGGATGGGATTATCAAATCTGCTGTGGGTATTGGCACGCTGCTTACAGAAGGTTTAGGAGA
CACTATTCGTTGTTCGTTAACAGGGTGTCCTACTGAAGAAATCCCTGTTTGTGAAAGTTTGTTAAAGCACACAACAATAT
ATCTTAATCTTCCTAAACAGGAAAATCCCTTTGCTTTAGAAAACTCGGAAAGTTTTGTCAATGCTTCTAAGAAAATTACA
AAAACAACCCCATGGGGATCTGTTTATGGAGTATTTATCAAGCTACACGAGCATCATATTTTAAACACGACCGCGGAAAG
GCTTCTTGAACAACTTGGTATAAACCCTACTAACGGGAAAAAAGATGCCACTGCACCCGAAGGCGTAGTTATTCCCAAAA
GTTTTTTGGGAACCTCTATTATTGAGAAGTTACAAAAGCACATGAGCGTTTTCCATCACCACGAAGTTCCCTGCCTGTAT
GACTATAATGAAGAAATCTGGAATAACGAGCAGGTTTTAAGCGCTCCCTTTGTACACTGTCACGCAACACCTCCGTTTAT
TCACAGCGTAAGATCTTTCTTTTCTAAAAGACAGTGTGAAGATCAGCCTGTGAAATTGGTATTTTCAAAAGATCTCGATG
ACGAATATGAAGCGACAGTATCTATAGCTACAGAATTTGGAGCTCTACTTCTTGATGGTTTAGGAGAGGGTGTAATTTTA
GACCTTCCCAATATTCCTCTACCCACTGTTCGAGAAATAGCCTTTGGCACTTTACAAAGTGCTGGCGTGCGTTTAGTGAA
AACAGAGTACATCTCATGTCCGGGATGTGGAAGAACTCTCTTCGATCTTCCCGAAGTAACAACACGAATTCGCAACAAAA
CAAAACATCTTGTAGGATTAAAAATCGCTGTTATGGGATGTATAGTTAATGGTCCTGGAGAAATGGCAGATGCTGATTTT
GGATTCGTTGGATCTAAAACAGGAATGATTGATCTCTATGTAAAGCATACGTGTGTAAAAGCACATATTCCCATGGAAGA
TGCCGAAGAAGAACTCTTTCGTCTTTTGCAAGAACATGGTGTATGGAAAGATCCCGAATAA

Upstream 100 bases:

>100_bases
AATAGGTTAAACTTTACCGTCGACTTTATTCTATGGATATTTTTCTCCCACTTCCAGGTAATGATTCTTCATTCCCGCAA
TAAAATTATTTTAGGTTTTT

Downstream 100 bases:

>100_bases
TACGTTTTAGCTATGACACAACCTATTTCTGACTATAGTTTAAATAAACTGACTTTCCCCGAACTCTCTGGCTTACCCTT
ACGGCATGGATTGTTTCCTA

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 606; Mature: 606

Protein sequence:

>606_residues
MIISPFKKQTARRYTHSVKIGSLFVGSEHSIKMQSMTTTPTSDVDATVAQICSLVEAKCDIARVTVQGVKEAQACEHIKE
RLLTMGLDIPLVADIHFFPQAAMHVADFVDKVRINPGNFVDKRNMFSGKTYTDKNYADSLLRLEEKFSPLVEKCKRLGKA
MRIGVNHGSLSERIMQRYGDTIEGMVVSALEYIKVCENLGYRDVVFSMKSSNPKVMVAAYRQLAKDLDARGWHYPLHLGV
TEAGMGMDGIIKSAVGIGTLLTEGLGDTIRCSLTGCPTEEIPVCESLLKHTTIYLNLPKQENPFALENSESFVNASKKIT
KTTPWGSVYGVFIKLHEHHILNTTAERLLEQLGINPTNGKKDATAPEGVVIPKSFLGTSIIEKLQKHMSVFHHHEVPCLY
DYNEEIWNNEQVLSAPFVHCHATPPFIHSVRSFFSKRQCEDQPVKLVFSKDLDDEYEATVSIATEFGALLLDGLGEGVIL
DLPNIPLPTVREIAFGTLQSAGVRLVKTEYISCPGCGRTLFDLPEVTTRIRNKTKHLVGLKIAVMGCIVNGPGEMADADF
GFVGSKTGMIDLYVKHTCVKAHIPMEDAEEELFRLLQEHGVWKDPE

Sequences:

>Translated_606_residues
MIISPFKKQTARRYTHSVKIGSLFVGSEHSIKMQSMTTTPTSDVDATVAQICSLVEAKCDIARVTVQGVKEAQACEHIKE
RLLTMGLDIPLVADIHFFPQAAMHVADFVDKVRINPGNFVDKRNMFSGKTYTDKNYADSLLRLEEKFSPLVEKCKRLGKA
MRIGVNHGSLSERIMQRYGDTIEGMVVSALEYIKVCENLGYRDVVFSMKSSNPKVMVAAYRQLAKDLDARGWHYPLHLGV
TEAGMGMDGIIKSAVGIGTLLTEGLGDTIRCSLTGCPTEEIPVCESLLKHTTIYLNLPKQENPFALENSESFVNASKKIT
KTTPWGSVYGVFIKLHEHHILNTTAERLLEQLGINPTNGKKDATAPEGVVIPKSFLGTSIIEKLQKHMSVFHHHEVPCLY
DYNEEIWNNEQVLSAPFVHCHATPPFIHSVRSFFSKRQCEDQPVKLVFSKDLDDEYEATVSIATEFGALLLDGLGEGVIL
DLPNIPLPTVREIAFGTLQSAGVRLVKTEYISCPGCGRTLFDLPEVTTRIRNKTKHLVGLKIAVMGCIVNGPGEMADADF
GFVGSKTGMIDLYVKHTCVKAHIPMEDAEEELFRLLQEHGVWKDPE
>Mature_606_residues
MIISPFKKQTARRYTHSVKIGSLFVGSEHSIKMQSMTTTPTSDVDATVAQICSLVEAKCDIARVTVQGVKEAQACEHIKE
RLLTMGLDIPLVADIHFFPQAAMHVADFVDKVRINPGNFVDKRNMFSGKTYTDKNYADSLLRLEEKFSPLVEKCKRLGKA
MRIGVNHGSLSERIMQRYGDTIEGMVVSALEYIKVCENLGYRDVVFSMKSSNPKVMVAAYRQLAKDLDARGWHYPLHLGV
TEAGMGMDGIIKSAVGIGTLLTEGLGDTIRCSLTGCPTEEIPVCESLLKHTTIYLNLPKQENPFALENSESFVNASKKIT
KTTPWGSVYGVFIKLHEHHILNTTAERLLEQLGINPTNGKKDATAPEGVVIPKSFLGTSIIEKLQKHMSVFHHHEVPCLY
DYNEEIWNNEQVLSAPFVHCHATPPFIHSVRSFFSKRQCEDQPVKLVFSKDLDDEYEATVSIATEFGALLLDGLGEGVIL
DLPNIPLPTVREIAFGTLQSAGVRLVKTEYISCPGCGRTLFDLPEVTTRIRNKTKHLVGLKIAVMGCIVNGPGEMADADF
GFVGSKTGMIDLYVKHTCVKAHIPMEDAEEELFRLLQEHGVWKDPE

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=284, Percent_Identity=37.6760563380282, Blast_Score=170, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_CHLFF (Q254D2)

Other databases:

- EMBL:   AP006861
- RefSeq:   YP_515501.1
- STRING:   Q254D2
- GeneID:   3959198
- GenomeReviews:   AP006861_GR
- KEGG:   cfe:CF0584
- eggNOG:   COG0821
- HOGENOM:   HBG335271
- OMA:   SMRIGTN
- ProtClustDB:   PRK00694
- BioCyc:   CFEL264202:CF0584-MONOMER
- HAMAP:   MF_00159
- InterPro:   IPR017178
- InterPro:   IPR004588
- PIRSF:   PIRSF037336
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE

EC number: =1.17.7.1

Molecular weight: Translated: 67280; Mature: 67280

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIISPFKKQTARRYTHSVKIGSLFVGSEHSIKMQSMTTTPTSDVDATVAQICSLVEAKCD
CCCCCHHHHHHHHHHHCEEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
IARVTVQGVKEAQACEHIKERLLTMGLDIPLVADIHFFPQAAMHVADFVDKVRINPGNFV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHEECCCCCCC
DKRNMFSGKTYTDKNYADSLLRLEEKFSPLVEKCKRLGKAMRIGVNHGSLSERIMQRYGD
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHCC
TIEGMVVSALEYIKVCENLGYRDVVFSMKSSNPKVMVAAYRQLAKDLDARGWHYPLHLGV
HHHHHHHHHHHHHHHHHCCCCHHEEEEECCCCCCEEHHHHHHHHHHHCCCCCEEEEEECC
TEAGMGMDGIIKSAVGIGTLLTEGLGDTIRCSLTGCPTEEIPVCESLLKHTTIYLNLPKQ
CCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHCCEEEEECCCC
ENPFALENSESFVNASKKITKTTPWGSVYGVFIKLHEHHILNTTAERLLEQLGINPTNGK
CCCCEECCCHHHHHHCHHHCCCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHCCCCCCCC
KDATAPEGVVIPKSFLGTSIIEKLQKHMSVFHHHEVPCLYDYNEEIWNNEQVLSAPFVHC
CCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHCCCCCEEECCEEEE
HATPPFIHSVRSFFSKRQCEDQPVKLVFSKDLDDEYEATVSIATEFGALLLDGLGEGVIL
CCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEE
DLPNIPLPTVREIAFGTLQSAGVRLVKTEYISCPGCGRTLFDLPEVTTRIRNKTKHLVGL
ECCCCCCCHHHHHHHHHHHHCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
KIAVMGCIVNGPGEMADADFGFVGSKTGMIDLYVKHTCVKAHIPMEDAEEELFRLLQEHG
EEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEEEEEEECCCCCCHHHHHHHHHHHCC
VWKDPE
CCCCCC
>Mature Secondary Structure
MIISPFKKQTARRYTHSVKIGSLFVGSEHSIKMQSMTTTPTSDVDATVAQICSLVEAKCD
CCCCCHHHHHHHHHHHCEEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
IARVTVQGVKEAQACEHIKERLLTMGLDIPLVADIHFFPQAAMHVADFVDKVRINPGNFV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHEECCCCCCC
DKRNMFSGKTYTDKNYADSLLRLEEKFSPLVEKCKRLGKAMRIGVNHGSLSERIMQRYGD
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHCC
TIEGMVVSALEYIKVCENLGYRDVVFSMKSSNPKVMVAAYRQLAKDLDARGWHYPLHLGV
HHHHHHHHHHHHHHHHHCCCCHHEEEEECCCCCCEEHHHHHHHHHHHCCCCCEEEEEECC
TEAGMGMDGIIKSAVGIGTLLTEGLGDTIRCSLTGCPTEEIPVCESLLKHTTIYLNLPKQ
CCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHCCEEEEECCCC
ENPFALENSESFVNASKKITKTTPWGSVYGVFIKLHEHHILNTTAERLLEQLGINPTNGK
CCCCEECCCHHHHHHCHHHCCCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHCCCCCCCC
KDATAPEGVVIPKSFLGTSIIEKLQKHMSVFHHHEVPCLYDYNEEIWNNEQVLSAPFVHC
CCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHCCCCCEEECCEEEE
HATPPFIHSVRSFFSKRQCEDQPVKLVFSKDLDDEYEATVSIATEFGALLLDGLGEGVIL
CCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEE
DLPNIPLPTVREIAFGTLQSAGVRLVKTEYISCPGCGRTLFDLPEVTTRIRNKTKHLVGL
ECCCCCCCHHHHHHHHHHHHCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
KIAVMGCIVNGPGEMADADFGFVGSKTGMIDLYVKHTCVKAHIPMEDAEEELFRLLQEHG
EEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEEEEEEECCCCCCHHHHHHHHHHHCC
VWKDPE
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA