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The map label for this gene is recF

Identifier: 89898370

GI number: 89898370

Start: 660513

End: 661616

Strand: Direct

Name: recF

Synonym: CF0563

Alternate gene names: 89898370

Gene position: 660513-661616 (Clockwise)

Preceding gene: 89898369

Following gene: 89898371

Centisome position: 56.64

GC content: 35.51

Gene sequence:

>1104_bases
ATGAATATTCTTTCCCTGCGTCTTAAGAATTTCAGAAATTATAAAGAAGCCAAGATTTCTTTATCTCCAAATATGAATTA
TATTTTTGGAGAAAATGCTCAGGGGAAAACTAATCTTCTTGAAGCTCTTTATGTTTTGTCTTTAGGCAGGTCATTTCGGA
CTTCCCATCTAACGGAAGCGATTTCCTTTGGCTCTGCATACTTCTTTTTAGAAATGACCTGTGAAAAAGACGGGTTTTCC
CATAAATTATCCATCTATGTAGATAAGCACGGGAAAAAAATCCTCAGCGATCAATCCCCCATAAAAACCCTGTCACAATT
AATAGGCATGGTGCCTATAGTTCTATTTTCCTCCAAAGATCGCTTGTTAATTTCTGGATCTCCTTCTGATCGAAGGCTTT
TTCTCAATTTGCTTTTATCGCAATGCGATCCCCAATATAAGCATACCCTTTCTTATTATCATCGAGCTTTACTACAAAGA
AACTCCCTACTTAAAACTAAACAAATCTCTACATTATCCGTTTGGGATGAGCAACTTGCCACTCTAGGTGCTTATCTAAC
ACTAAGTCGGTTTACTTGTTGCGAACAACTGAATCAGTTAGTTCAAAAATTATGGAATAATTCTTTATCCGAACATATAC
GTATAAAATTTAAAAGCTCGCTAATTAAACAGGACAAGCTTTCTAAAGAATCTATTATTGAAGAACTTCGCAAACAGCTA
ACATCTTCTCTTCATCGCGATTTAGAATTAGGAAGCACATCTGTTGGCCCACATCGAGAAGACTTTACTTTAATGATTAA
TGACCTTCCCGTATCACAATTTTCCAGTGAGGGTCAAAAACATAGTCTGCTTGCAATTCTTAGACTCGCAGAATGTCTCT
ATATAAAAAACATTCACAACGTATGTCCCTTATTCTGTATGGATGATATTCATTCAGGATTGGACAATCACAGAATCTCT
CAATTATTAGATCTAGCTCCTACTCTAGGACAAACTCTAATGACGTCTACACACCTTCCTCATCAAACATTATCCAAAAA
TAGTCGGATATTTTTAGTTAATCAAGCTCAAGTATCTATTCATTCGCATTCCATTATTAAATAG

Upstream 100 bases:

>100_bases
TAGTTCGATTAGGCATTTCAGACTCTTATAATCCTGGGATTATTACTGATTCCACACGCAGCCTATTCGTTATTATGCCA
ATGAGATTGCATGATGATTA

Downstream 100 bases:

>100_bases
TCGTGTTTTTATTAATTCATAATTAGTCGGTTAATTATTTTTTAAAATAAGTTAATTAACGCGCTGGTTTTTAATAAAAA
ACTTATTAAAATAATTATAT

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MNILSLRLKNFRNYKEAKISLSPNMNYIFGENAQGKTNLLEALYVLSLGRSFRTSHLTEAISFGSAYFFLEMTCEKDGFS
HKLSIYVDKHGKKILSDQSPIKTLSQLIGMVPIVLFSSKDRLLISGSPSDRRLFLNLLLSQCDPQYKHTLSYYHRALLQR
NSLLKTKQISTLSVWDEQLATLGAYLTLSRFTCCEQLNQLVQKLWNNSLSEHIRIKFKSSLIKQDKLSKESIIEELRKQL
TSSLHRDLELGSTSVGPHREDFTLMINDLPVSQFSSEGQKHSLLAILRLAECLYIKNIHNVCPLFCMDDIHSGLDNHRIS
QLLDLAPTLGQTLMTSTHLPHQTLSKNSRIFLVNQAQVSIHSHSIIK

Sequences:

>Translated_367_residues
MNILSLRLKNFRNYKEAKISLSPNMNYIFGENAQGKTNLLEALYVLSLGRSFRTSHLTEAISFGSAYFFLEMTCEKDGFS
HKLSIYVDKHGKKILSDQSPIKTLSQLIGMVPIVLFSSKDRLLISGSPSDRRLFLNLLLSQCDPQYKHTLSYYHRALLQR
NSLLKTKQISTLSVWDEQLATLGAYLTLSRFTCCEQLNQLVQKLWNNSLSEHIRIKFKSSLIKQDKLSKESIIEELRKQL
TSSLHRDLELGSTSVGPHREDFTLMINDLPVSQFSSEGQKHSLLAILRLAECLYIKNIHNVCPLFCMDDIHSGLDNHRIS
QLLDLAPTLGQTLMTSTHLPHQTLSKNSRIFLVNQAQVSIHSHSIIK
>Mature_367_residues
MNILSLRLKNFRNYKEAKISLSPNMNYIFGENAQGKTNLLEALYVLSLGRSFRTSHLTEAISFGSAYFFLEMTCEKDGFS
HKLSIYVDKHGKKILSDQSPIKTLSQLIGMVPIVLFSSKDRLLISGSPSDRRLFLNLLLSQCDPQYKHTLSYYHRALLQR
NSLLKTKQISTLSVWDEQLATLGAYLTLSRFTCCEQLNQLVQKLWNNSLSEHIRIKFKSSLIKQDKLSKESIIEELRKQL
TSSLHRDLELGSTSVGPHREDFTLMINDLPVSQFSSEGQKHSLLAILRLAECLYIKNIHNVCPLFCMDDIHSGLDNHRIS
QLLDLAPTLGQTLMTSTHLPHQTLSKNSRIFLVNQAQVSIHSHSIIK

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=364, Percent_Identity=26.9230769230769, Blast_Score=131, Evalue=6e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_CHLFF (Q254F3)

Other databases:

- EMBL:   AP006861
- RefSeq:   YP_515480.1
- STRING:   Q254F3
- GeneID:   3958900
- GenomeReviews:   AP006861_GR
- KEGG:   cfe:CF0563
- eggNOG:   COG1195
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- ProtClustDB:   PRK00064
- BioCyc:   CFEL264202:CF0563-MONOMER
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 41818; Mature: 41818

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILSLRLKNFRNYKEAKISLSPNMNYIFGENAQGKTNLLEALYVLSLGRSFRTSHLTEA
CCHHHHHHHHHCCCCCEEEEECCCCCEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHH
ISFGSAYFFLEMTCEKDGFSHKLSIYVDKHGKKILSDQSPIKTLSQLIGMVPIVLFSSKD
HHCCCEEEEEEEEECCCCCCEEEEEEEECCCCHHHCCCCHHHHHHHHHHHHHEEEECCCC
RLLISGSPSDRRLFLNLLLSQCDPQYKHTLSYYHRALLQRNSLLKTKQISTLSVWDEQLA
CEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHH
TLGAYLTLSRFTCCEQLNQLVQKLWNNSLSEHIRIKFKSSLIKQDKLSKESIIEELRKQL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEEHHHHHHHHHHCCCHHHHHHHHHHHH
TSSLHRDLELGSTSVGPHREDFTLMINDLPVSQFSSEGQKHSLLAILRLAECLYIKNIHN
HHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
VCPLFCMDDIHSGLDNHRISQLLDLAPTLGQTLMTSTHLPHQTLSKNSRIFLVNQAQVSI
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEEECCEEEE
HSHSIIK
CCCCCCC
>Mature Secondary Structure
MNILSLRLKNFRNYKEAKISLSPNMNYIFGENAQGKTNLLEALYVLSLGRSFRTSHLTEA
CCHHHHHHHHHCCCCCEEEEECCCCCEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHH
ISFGSAYFFLEMTCEKDGFSHKLSIYVDKHGKKILSDQSPIKTLSQLIGMVPIVLFSSKD
HHCCCEEEEEEEEECCCCCCEEEEEEEECCCCHHHCCCCHHHHHHHHHHHHHEEEECCCC
RLLISGSPSDRRLFLNLLLSQCDPQYKHTLSYYHRALLQRNSLLKTKQISTLSVWDEQLA
CEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHH
TLGAYLTLSRFTCCEQLNQLVQKLWNNSLSEHIRIKFKSSLIKQDKLSKESIIEELRKQL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEEHHHHHHHHHHCCCHHHHHHHHHHHH
TSSLHRDLELGSTSVGPHREDFTLMINDLPVSQFSSEGQKHSLLAILRLAECLYIKNIHN
HHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
VCPLFCMDDIHSGLDNHRISQLLDLAPTLGQTLMTSTHLPHQTLSKNSRIFLVNQAQVSI
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEEECCEEEE
HSHSIIK
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA