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The map label for this gene is nusA [H]

Identifier: 89898348

GI number: 89898348

Start: 636085

End: 637389

Strand: Direct

Name: nusA [H]

Synonym: CF0541

Alternate gene names: 89898348

Gene position: 636085-637389 (Clockwise)

Preceding gene: 89898347

Following gene: 89898349

Centisome position: 54.54

GC content: 37.7

Gene sequence:

>1305_bases
ATGAATAAAGATCTTGTAGCTATTTTTGACTACATGGAGAAAGAAAAAGGAATTCAACGTCCTGTTATCATAGGCGCAAT
TGAATCGGCCTTAAAAATTGCAGCAAAAAAAACACTAAGAGATGATGCTAACGTTTCTGTAAATATTAATCCTCGAACTG
GCGATATAGAAGTCTTTTGCGAAAAAGAAATCGTAGAAGTATGTGAAAATCCCAGCAAAGAGATTCCCTTAGACAAAGCT
AGAGAGTACGATCCTGAATGCCAAATTGGGCAATACATGGATGTTCCCTTTGTTTCAGAGCATTTTGGCAGAATTGCAGC
TCATGCGGCTCGGCAAATTATAGGTCAAAAATTACGTCATGCTGAAAGGGATGTTATCTATGAAGAATATCGGCATCGGG
TCAATGAAATCCTTTCTGGTGTTGTCAAGCGCTTTGCCAAAGGATCAAATTTAGTCATAGATTTAGGGAAAGTTGAAGGT
CTGTTACCTGCTCGTTACTATCCTAAAACAGAGAAGCATAAGGTTGGCGATAAAATTTACGCCTTATTATATGAAGTTCA
AGAATCTGAAAATGGTGGTGCTGAAGTTATTCTTAGCCGCAGCCATCCAGAATTCGTTAAACAACTTTTCCTACAAGAAG
TTCCTGAATTAGAAGATGGCTCTGTAGAAATTATTAAAATTGCTAGAGAAGCTGGTTATAGAACTAAATTAGCTGTAAGT
TCAACCGACCCCAAAACAGATCCTGTAGGAGCGTTTGTTGGGATGAGAGGTTCTCGAGTGAAGAATATCATCCGAGAATT
AAATGACGAGAAAATAGATATTGTAAACTATTCTCCCGTAACCACAGAATTATTACAAAATTTGCTTTGCCCCATAGAAA
TTCAAAAGATTGCGATCTTAGAAGATGACAAGGTTATTGCTATAGTCGTTCATGACGCTGATTACGCAACAGTAATTGGC
AAACGTGGAATTAATGCTCGATTGATTAGTCAAATTTTAGACTATGAGCTCGAAGTTCAACGTATGAGCGAGTACAATAA
ACTATTAGAAATTCAACGCCTACAGTTAGCGGAATTTGATAATCCTCAGTTAGATGAACCGCTGGATATGGAAGGCATTA
GTAAGTTGGTTGTCCAAAATCTTGTACACGCAGGATACGACACGATCAGAAAGGTATTGTTAGCCAGTGCTAATGATCTT
GCTTCTGTTCCTGGAATCAGTTTAGAACTTGCTTATAAGATCCTTGAGCAAGTCAGCAAATATGGAGAAGGCAAAGTTGA
CGAAAAACCTAAAATTGAAGATTAA

Upstream 100 bases:

>100_bases
GGAAATAATATTACTCAAGAGGTTGGGCTTATGTTTCTTGCAACATAAGCTCGCCTTGGTTTAGCACTCTGTCTGTTTCC
TTATTTATAAGAGGAGTATA

Downstream 100 bases:

>100_bases
AAACGCTCAATTAACGAAAGCTGCTGGATTAGATAAGTTAAAACAAAAATTAGCTCAGGCAGGATCTTCAGACACAAAAA
ATTCTTCAGAAAAGCCTTCT

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MNKDLVAIFDYMEKEKGIQRPVIIGAIESALKIAAKKTLRDDANVSVNINPRTGDIEVFCEKEIVEVCENPSKEIPLDKA
REYDPECQIGQYMDVPFVSEHFGRIAAHAARQIIGQKLRHAERDVIYEEYRHRVNEILSGVVKRFAKGSNLVIDLGKVEG
LLPARYYPKTEKHKVGDKIYALLYEVQESENGGAEVILSRSHPEFVKQLFLQEVPELEDGSVEIIKIAREAGYRTKLAVS
STDPKTDPVGAFVGMRGSRVKNIIRELNDEKIDIVNYSPVTTELLQNLLCPIEIQKIAILEDDKVIAIVVHDADYATVIG
KRGINARLISQILDYELEVQRMSEYNKLLEIQRLQLAEFDNPQLDEPLDMEGISKLVVQNLVHAGYDTIRKVLLASANDL
ASVPGISLELAYKILEQVSKYGEGKVDEKPKIED

Sequences:

>Translated_434_residues
MNKDLVAIFDYMEKEKGIQRPVIIGAIESALKIAAKKTLRDDANVSVNINPRTGDIEVFCEKEIVEVCENPSKEIPLDKA
REYDPECQIGQYMDVPFVSEHFGRIAAHAARQIIGQKLRHAERDVIYEEYRHRVNEILSGVVKRFAKGSNLVIDLGKVEG
LLPARYYPKTEKHKVGDKIYALLYEVQESENGGAEVILSRSHPEFVKQLFLQEVPELEDGSVEIIKIAREAGYRTKLAVS
STDPKTDPVGAFVGMRGSRVKNIIRELNDEKIDIVNYSPVTTELLQNLLCPIEIQKIAILEDDKVIAIVVHDADYATVIG
KRGINARLISQILDYELEVQRMSEYNKLLEIQRLQLAEFDNPQLDEPLDMEGISKLVVQNLVHAGYDTIRKVLLASANDL
ASVPGISLELAYKILEQVSKYGEGKVDEKPKIED
>Mature_434_residues
MNKDLVAIFDYMEKEKGIQRPVIIGAIESALKIAAKKTLRDDANVSVNINPRTGDIEVFCEKEIVEVCENPSKEIPLDKA
REYDPECQIGQYMDVPFVSEHFGRIAAHAARQIIGQKLRHAERDVIYEEYRHRVNEILSGVVKRFAKGSNLVIDLGKVEG
LLPARYYPKTEKHKVGDKIYALLYEVQESENGGAEVILSRSHPEFVKQLFLQEVPELEDGSVEIIKIAREAGYRTKLAVS
STDPKTDPVGAFVGMRGSRVKNIIRELNDEKIDIVNYSPVTTELLQNLLCPIEIQKIAILEDDKVIAIVVHDADYATVIG
KRGINARLISQILDYELEVQRMSEYNKLLEIQRLQLAEFDNPQLDEPLDMEGISKLVVQNLVHAGYDTIRKVLLASANDL
ASVPGISLELAYKILEQVSKYGEGKVDEKPKIED

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=479, Percent_Identity=31.7327766179541, Blast_Score=223, Evalue=2e-59,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 48878; Mature: 48878

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKDLVAIFDYMEKEKGIQRPVIIGAIESALKIAAKKTLRDDANVSVNINPRTGDIEVFC
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEE
EKEIVEVCENPSKEIPLDKAREYDPECQIGQYMDVPFVSEHFGRIAAHAARQIIGQKLRH
HHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AERDVIYEEYRHRVNEILSGVVKRFAKGSNLVIDLGKVEGLLPARYYPKTEKHKVGDKIY
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHH
ALLYEVQESENGGAEVILSRSHPEFVKQLFLQEVPELEDGSVEIIKIAREAGYRTKLAVS
HHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCEEHHHHHCCCEEEEEEE
STDPKTDPVGAFVGMRGSRVKNIIRELNDEKIDIVNYSPVTTELLQNLLCPIEIQKIAIL
CCCCCCCCCHHHHCCCCHHHHHHHHHCCCCEEEEEECCCHHHHHHHHCCCCCEEEEEEEE
EDDKVIAIVVHDADYATVIGKRGINARLISQILDYELEVQRMSEYNKLLEIQRLQLAEFD
ECCCEEEEEEECCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NPQLDEPLDMEGISKLVVQNLVHAGYDTIRKVLLASANDLASVPGISLELAYKILEQVSK
CCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHH
YGEGKVDEKPKIED
HCCCCCCCCCCCCC
>Mature Secondary Structure
MNKDLVAIFDYMEKEKGIQRPVIIGAIESALKIAAKKTLRDDANVSVNINPRTGDIEVFC
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEE
EKEIVEVCENPSKEIPLDKAREYDPECQIGQYMDVPFVSEHFGRIAAHAARQIIGQKLRH
HHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AERDVIYEEYRHRVNEILSGVVKRFAKGSNLVIDLGKVEGLLPARYYPKTEKHKVGDKIY
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHH
ALLYEVQESENGGAEVILSRSHPEFVKQLFLQEVPELEDGSVEIIKIAREAGYRTKLAVS
HHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCEEHHHHHCCCEEEEEEE
STDPKTDPVGAFVGMRGSRVKNIIRELNDEKIDIVNYSPVTTELLQNLLCPIEIQKIAIL
CCCCCCCCCHHHHCCCCHHHHHHHHHCCCCEEEEEECCCHHHHHHHHCCCCCEEEEEEEE
EDDKVIAIVVHDADYATVIGKRGINARLISQILDYELEVQRMSEYNKLLEIQRLQLAEFD
ECCCEEEEEEECCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NPQLDEPLDMEGISKLVVQNLVHAGYDTIRKVLLASANDLASVPGISLELAYKILEQVSK
CCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHH
YGEGKVDEKPKIED
HCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]