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The map label for this gene is rimO

Identifier: 89898289

GI number: 89898289

Start: 567964

End: 569346

Strand: Direct

Name: rimO

Synonym: CF0482

Alternate gene names: 89898289

Gene position: 567964-569346 (Clockwise)

Preceding gene: 89898286

Following gene: 89898294

Centisome position: 48.7

GC content: 37.45

Gene sequence:

>1383_bases
ATGAAAATTAAGGAACAGTTTTTTTTTAAACAGGAAGCTTCTAAAAATAAAATTCATTTTATTAGTTTGGGCTGCTCTAG
AAATCTTGTGGACACGGAGGTAATGCTCGGGATTTTATTAAAGTCTGGGTATGAAGCCACCGAATCTCTTGAAGAAGCCG
ACTATTTAATTTTAAATACTTGTGCATTTTTAAAAGCTGCTCGCGATGAATCTACGGACTATCTTCAACGTATAATTAAG
GCAAAAAAAGAAACAGCTAAGATTATATTAACGGGCTGCATGGTTTCTAAACATAAGGAGGAGTTGAAACCCTTGCTTCC
CCATATACACTATGTCTTAGGTTCTGGGGATGTTGAACATATTCTATCTGCTATTGAATCTAAAGAATATGGGGAAAAAA
TCTCTTCTAAAAGTTATTTAGAAATGGGCGAGATTCCGAGAAAGTTGTCGACACCCAAACACTATGCTTATTTAAAAATA
GCTGAGGGATGTCGCAAGCGCTGTGCTTTTTGCATAATTCCTACTATTAAAGGTGCTTTAAGAAGCAAGCCTCTGGATCA
AATTATTAAAGAGTTTCGCCTATTGTTAAAAATGGGGGTTAAGGAAATCATTTTAATCGCTCAAGACTTAGGTGACTATG
GCAAAGACTTCTCTGCGGATCGGAAATCTTGTCTAGATAAAGTACTAAAAGAGATGCTCAAAGAGCCGGGGGATTATTGG
ATTAGGATGCTCTATTTATATCCTGACGAAGTGGATGATACTATAATTGATCTTATGGAAAGCGATCACCGCCTTCTCCC
CTATGTTGATATTCCTCTTCAACATATCAATAATCGGGTCTTAAAAAAGATGTTGAGAACAACGTCTAAAGAGCAAATTC
TAGATTTATTAACCAAACTTCGCACACGGATTCCACATATTTATATCCGTTCATCATTTATTGTAGGATTCCCTGGAGAA
ACCGATGACGAATTTCAAGATCTTGTAGACTTCGTTAGAGAGGGATGGATCGATAATCTAGGTATCTTTTCCTATTCTCA
AGAAGAAGGTTCTGTAGCAGCTGACATGCCCGATCAAGTATCTCAGAGTGTAAAATCCAAGAGATTAAAAATTCTTTCTC
AAGCTCAAAAGCAAAATGTTGAGAAACATAATCAGAAGCTTGTGGGGCAAGTTGTTGAAGCTGTCATTGATGGGTATCAT
CCGGATAGTGAGCTTTTATTGACAGCACGTTTTTATGGACAGGCTCCAGAAGTTGATCCTTGCATTATTGTAAACGAAGC
TCGTTTAGTTTCCGGATTTGGTGAGCGTTACTTAATCGAAATTACAGGTTATGTAGGTTATGATCTTATAGGTCGAGTTG
TGAAAAAGGCGCCCGGAGAATAG

Upstream 100 bases:

>100_bases
ATTGATGAATCCTTTTAAGAAAATGATAAATCGCTTATGATACTATATTTATTTTCAAAAAGAATCAAGAAAACTTCCCC
ATCATTACAAGAGAATCTTC

Downstream 100 bases:

>100_bases
TTTCCGGGCAAAATAGGATTCGGTATTTTATTCAAAATAAGGTAATTGAACTACATTCCAAGAAAATGATGCAGCACGCC
ATGAATAGGTTATATTTTTT

Product: hypothetical protein

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MKIKEQFFFKQEASKNKIHFISLGCSRNLVDTEVMLGILLKSGYEATESLEEADYLILNTCAFLKAARDESTDYLQRIIK
AKKETAKIILTGCMVSKHKEELKPLLPHIHYVLGSGDVEHILSAIESKEYGEKISSKSYLEMGEIPRKLSTPKHYAYLKI
AEGCRKRCAFCIIPTIKGALRSKPLDQIIKEFRLLLKMGVKEIILIAQDLGDYGKDFSADRKSCLDKVLKEMLKEPGDYW
IRMLYLYPDEVDDTIIDLMESDHRLLPYVDIPLQHINNRVLKKMLRTTSKEQILDLLTKLRTRIPHIYIRSSFIVGFPGE
TDDEFQDLVDFVREGWIDNLGIFSYSQEEGSVAADMPDQVSQSVKSKRLKILSQAQKQNVEKHNQKLVGQVVEAVIDGYH
PDSELLLTARFYGQAPEVDPCIIVNEARLVSGFGERYLIEITGYVGYDLIGRVVKKAPGE

Sequences:

>Translated_460_residues
MKIKEQFFFKQEASKNKIHFISLGCSRNLVDTEVMLGILLKSGYEATESLEEADYLILNTCAFLKAARDESTDYLQRIIK
AKKETAKIILTGCMVSKHKEELKPLLPHIHYVLGSGDVEHILSAIESKEYGEKISSKSYLEMGEIPRKLSTPKHYAYLKI
AEGCRKRCAFCIIPTIKGALRSKPLDQIIKEFRLLLKMGVKEIILIAQDLGDYGKDFSADRKSCLDKVLKEMLKEPGDYW
IRMLYLYPDEVDDTIIDLMESDHRLLPYVDIPLQHINNRVLKKMLRTTSKEQILDLLTKLRTRIPHIYIRSSFIVGFPGE
TDDEFQDLVDFVREGWIDNLGIFSYSQEEGSVAADMPDQVSQSVKSKRLKILSQAQKQNVEKHNQKLVGQVVEAVIDGYH
PDSELLLTARFYGQAPEVDPCIIVNEARLVSGFGERYLIEITGYVGYDLIGRVVKKAPGE
>Mature_460_residues
MKIKEQFFFKQEASKNKIHFISLGCSRNLVDTEVMLGILLKSGYEATESLEEADYLILNTCAFLKAARDESTDYLQRIIK
AKKETAKIILTGCMVSKHKEELKPLLPHIHYVLGSGDVEHILSAIESKEYGEKISSKSYLEMGEIPRKLSTPKHYAYLKI
AEGCRKRCAFCIIPTIKGALRSKPLDQIIKEFRLLLKMGVKEIILIAQDLGDYGKDFSADRKSCLDKVLKEMLKEPGDYW
IRMLYLYPDEVDDTIIDLMESDHRLLPYVDIPLQHINNRVLKKMLRTTSKEQILDLLTKLRTRIPHIYIRSSFIVGFPGE
TDDEFQDLVDFVREGWIDNLGIFSYSQEEGSVAADMPDQVSQSVKSKRLKILSQAQKQNVEKHNQKLVGQVVEAVIDGYH
PDSELLLTARFYGQAPEVDPCIIVNEARLVSGFGERYLIEITGYVGYDLIGRVVKKAPGE

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872782, Length=479, Percent_Identity=25.4697286012526, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI28872784, Length=479, Percent_Identity=25.4697286012526, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI93277076, Length=371, Percent_Identity=28.0323450134771, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI1787057, Length=447, Percent_Identity=37.3601789709172, Blast_Score=273, Evalue=2e-74,
Organism=Escherichia coli, GI1786882, Length=448, Percent_Identity=28.5714285714286, Blast_Score=172, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17553146, Length=419, Percent_Identity=23.1503579952267, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI71996771, Length=276, Percent_Identity=25.7246376811594, Blast_Score=88, Evalue=9e-18,
Organism=Drosophila melanogaster, GI21356207, Length=466, Percent_Identity=27.4678111587983, Blast_Score=174, Evalue=8e-44,
Organism=Drosophila melanogaster, GI19922432, Length=372, Percent_Identity=24.4623655913978, Blast_Score=106, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_CHLFF (Q254N4)

Other databases:

- EMBL:   AP006861
- RefSeq:   YP_515399.1
- ProteinModelPortal:   Q254N4
- SMR:   Q254N4
- STRING:   Q254N4
- GeneID:   3959130
- GenomeReviews:   AP006861_GR
- KEGG:   cfe:CF0482
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   LYLYPDE
- ProtClustDB:   CLSK401459
- BioCyc:   CFEL264202:CF0482-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 52447; Mature: 52447

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM; PS00086 CYTOCHROME_P450

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKEQFFFKQEASKNKIHFISLGCSRNLVDTEVMLGILLKSGYEATESLEEADYLILNT
CCCHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHH
CAFLKAARDESTDYLQRIIKAKKETAKIILTGCMVSKHKEELKPLLPHIHYVLGSGDVEH
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
ILSAIESKEYGEKISSKSYLEMGEIPRKLSTPKHYAYLKIAEGCRKRCAFCIIPTIKGAL
HHHHHHHHHHHHHHCCCHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHCCEEEEHHHHHHH
RSKPLDQIIKEFRLLLKMGVKEIILIAQDLGDYGKDFSADRKSCLDKVLKEMLKEPGDYW
HCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHH
IRMLYLYPDEVDDTIIDLMESDHRLLPYVDIPLQHINNRVLKKMLRTTSKEQILDLLTKL
EEEEEECCCCHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHH
RTRIPHIYIRSSFIVGFPGETDDEFQDLVDFVREGWIDNLGIFSYSQEEGSVAADMPDQV
HHHCCEEEEECEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCEECCCHHHH
SQSVKSKRLKILSQAQKQNVEKHNQKLVGQVVEAVIDGYHPDSELLLTARFYGQAPEVDP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCC
CIIVNEARLVSGFGERYLIEITGYVGYDLIGRVVKKAPGE
EEEECCHHHHHCCCCEEEEEEECHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKIKEQFFFKQEASKNKIHFISLGCSRNLVDTEVMLGILLKSGYEATESLEEADYLILNT
CCCHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHH
CAFLKAARDESTDYLQRIIKAKKETAKIILTGCMVSKHKEELKPLLPHIHYVLGSGDVEH
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
ILSAIESKEYGEKISSKSYLEMGEIPRKLSTPKHYAYLKIAEGCRKRCAFCIIPTIKGAL
HHHHHHHHHHHHHHCCCHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHCCEEEEHHHHHHH
RSKPLDQIIKEFRLLLKMGVKEIILIAQDLGDYGKDFSADRKSCLDKVLKEMLKEPGDYW
HCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHH
IRMLYLYPDEVDDTIIDLMESDHRLLPYVDIPLQHINNRVLKKMLRTTSKEQILDLLTKL
EEEEEECCCCHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHH
RTRIPHIYIRSSFIVGFPGETDDEFQDLVDFVREGWIDNLGIFSYSQEEGSVAADMPDQV
HHHCCEEEEECEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCEECCCHHHH
SQSVKSKRLKILSQAQKQNVEKHNQKLVGQVVEAVIDGYHPDSELLLTARFYGQAPEVDP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCC
CIIVNEARLVSGFGERYLIEITGYVGYDLIGRVVKKAPGE
EEEECCHHHHHCCCCEEEEEEECHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA