Click here to switch to the map view.

The map label for this gene is clpB1 [H]

Identifier: 89898185

GI number: 89898185

Start: 441427

End: 444021

Strand: Direct

Name: clpB1 [H]

Synonym: CF0378

Alternate gene names: 89898185

Gene position: 441427-444021 (Clockwise)

Preceding gene: 89898184

Following gene: 89898188

Centisome position: 37.85

GC content: 40.15

Gene sequence:

>2595_bases
ATGGATAAGATTTCGGATGCGGTAAGCGAAGCTCTAGAAAAAGCTTTTGAGCTCGCTAAATCAGCAAAAAATCCTTATGT
AAGTGAAAACCATTTTCTTAAATGTCTTTTAGAAAATACTGAGTCATTATTTTATTTGATAATAAAAGACATACAAAGCA
ACCCCAAATTACTTATTACTGCAGTGGACGAGGCCCTTTCTCTAGAGCCCTCTGTTGTCAGTGGAGATGCTATGCCTAAG
CCTTCTCCAGGGCTACAAAGTCTACTTTTAGATGCTAGGCAAACGGCTAAAGATCTCGGAGATGAGTACCTTTCTGGAGA
TCATGTACTCCTAGCTTTTTGGAAATCCAATAAGGAGCCTTTCGCTTCTTGGAAAAAAACGGTAAAAGTATCCTTAGATG
ATCTCAAGAAACTCATTATTAATATAAGGCGTGGAAATCGTATGGACTCTCCTAGCGCAGAAAATAATCTTCGGGGCCTA
GAAAAGTATTGTAAGAACTTAACCTCGTTAGCAAAAGATGGAAAATTAGATCCTGTGATTGGTAGGGACGAAGAAATTCG
TCGTACAGTTCAAGTTTTATCACGAAGAACTAAAAATAACCCCATGCTTATAGGTGAGCCTGGAGTTGGGAAAACAGCAA
TTGCTGAGGGGCTGGCCCTGCGTATTATTCAGGGGGATGTGCCAGAAAGCTTAAAAGGTAAACAGCTCTACGTTTTGGAT
ATGGGAGCATTAATTGCCGGAGCAAAATATCGCGGAGAATTCGAAGAGCGCTTAAAAAGTGTGCTTAAAGATGTAGAGTC
TGTGGATGGTGAGAGTATTTTATTCATTGATGAAGTGCATACTCTTGTAGGTGCTGGAGCTACTGATGGTGCTATGGATG
CTGCAAATTTATTAAAGCCCGCTTTAGCAAGAGGTACTCTGCATTGTATCGGAGCTACAACTCTTAATGAATACCAAAAA
TATATAGAAAAAGATGCCGCTTTAGAACGGCGTTTCCAGCCTATTTTTGTAACAGAACCTTCTTTAGAAGACGCCGTGTT
TATTCTTCGCGGTCTTCGTGAGAAGTATGAAATTTTCCATGGCGTGCGTATTACTGAGGGAGCATTAAATGCTGCCGTTC
TTCTTTCTTATAGATATATTCCCGATCGTTTTCTTCCAGATAAGGCGATTGACTTGATAGACGAGGCTGCAAGTTTAATT
CGCATGCAAATCGGAAGTTTGCCCCTGCCTATCGATGAGAAAGAGCGGGAACTCGCTGCTTTGATTGTTAAGCAAGAAGC
CATAAAACGTGAAAAAGCTCCTTCATATCAAGAAGAAGCGGAGGCTATGCAGCAGTCTATAGATCAGCTTAAGGAAGAGT
TGGCAGTTTTGCGTCTGCGTTGGGATGAGGAGAAAAAACTTATCTCAGGCCTTAAGGAGAAAAAGAATTCTTTAGAAAAT
ATGAAGTTTTCTGAAGAAGAAGCTGAACGCGTTGCTGATTACAATCGTGTAGCAGAGCTACGCTATAGTTTAATTCCTGC
TCTTGAAGAAGAAATTCGTCAAGATGAAGAAGCTTTAAATCAAAGAGACAATCGCCTTCTTCAAGAAGAAGTGGATGAGC
GTCTTATTGCTCAGGTAGTGGCAAATTGGACAGGAATTCCTGTTCAGAAAATGTTAGAAGGCGAAGCCGAGAAGCTTCTC
GTTTTAGAAGAGTCTTTAGAAGAGCGCGTTGTTGGTCAGCCATTTGCTATTGCAGCTGTTAGTGATTCTATACGTGCTGC
CCGTGTAGGACTTAGTGATCCTCAACGACCTTTAGGAGTTTTCTTATTTTTGGGGCCTACGGGAGTGGGGAAAACAGAAC
TTGCTAAAGCTCTCGCAGATCTTTTGTTTAATAAAGAAGAGGCGATGGTACGCTTTGACATGACCGAATATATGGAAAAA
CATTCCGTATCCAAATTGATAGGATCGCCTCCAGGTTATGTAGGTTACGAAGAAGGGGGAAGTCTTTCTGAAGCTTTACG
TCGACGTCCTTATTCCGTCGTGCTGTTTGATGAAATTGAAAAAGCAGATAAAGAAGTATTTAATATTCTCTTGCAAATCT
TTGATGAAGGCATTCTTACGGATAGTAAAAAGCGTAAGGTAAATTGTAAAAATGCCTTATTCATTATGACTTCAAATATA
GGTTCTCAAGAACTTGCCGATTACTGCGCTAAGAAGGGAAGAGAGGTAAGTAAGGAGACTGTATTATCGGTAGTTTCCCC
AGAATTAAAGAAATACTTCAGTCCAGAGTTTATCAATCGTATTGATGATATTCTTCCTTTTGTTCCTTTGAATACCGAAG
ATATTGTAAAAATTGTCGGTATTCAGATGCGTAGAGTGGCTCAACGCTTGTTAGAAAGACGTGTAACATTAACTTGGGAT
GATTCTGTGATACTCTATTTAAGTGAGCACGGTTATGACAGTTCCTTTGGAGCACGGCCCTTAAAACGGTTAATTCAACA
GAAGGTAGTCACCTTGTTATCAAAAGCTTTGCTTAAAGGCGATATTAAATCAGATACGTCTATAGAGCTTACAATGTCTC
GGGATGTCTTGCTTTTCAAAAAAGTTAGCGGTTAG

Upstream 100 bases:

>100_bases
GAGTGAGGAACAACAACAAGTTGCTCTGGAAAATAGGTAGATTTTTTGTTTTCTCTATGCAATGAATAAAATGTTTTCAG
CAGAGTGTTAAGGCTGTTTT

Downstream 100 bases:

>100_bases
TCATTAGTATAGCCGTTTGTTACTGTGATTCTAGATCCTCTTGCTCAAGTATTTTTTTTAGAGCAGAGGGTCTTTTTTTA
TGCTTCAAATACATAATGTT

Product: ATP-dependent Clp endopeptidase ATP-binding chain B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 864; Mature: 864

Protein sequence:

>864_residues
MDKISDAVSEALEKAFELAKSAKNPYVSENHFLKCLLENTESLFYLIIKDIQSNPKLLITAVDEALSLEPSVVSGDAMPK
PSPGLQSLLLDARQTAKDLGDEYLSGDHVLLAFWKSNKEPFASWKKTVKVSLDDLKKLIINIRRGNRMDSPSAENNLRGL
EKYCKNLTSLAKDGKLDPVIGRDEEIRRTVQVLSRRTKNNPMLIGEPGVGKTAIAEGLALRIIQGDVPESLKGKQLYVLD
MGALIAGAKYRGEFEERLKSVLKDVESVDGESILFIDEVHTLVGAGATDGAMDAANLLKPALARGTLHCIGATTLNEYQK
YIEKDAALERRFQPIFVTEPSLEDAVFILRGLREKYEIFHGVRITEGALNAAVLLSYRYIPDRFLPDKAIDLIDEAASLI
RMQIGSLPLPIDEKERELAALIVKQEAIKREKAPSYQEEAEAMQQSIDQLKEELAVLRLRWDEEKKLISGLKEKKNSLEN
MKFSEEEAERVADYNRVAELRYSLIPALEEEIRQDEEALNQRDNRLLQEEVDERLIAQVVANWTGIPVQKMLEGEAEKLL
VLEESLEERVVGQPFAIAAVSDSIRAARVGLSDPQRPLGVFLFLGPTGVGKTELAKALADLLFNKEEAMVRFDMTEYMEK
HSVSKLIGSPPGYVGYEEGGSLSEALRRRPYSVVLFDEIEKADKEVFNILLQIFDEGILTDSKKRKVNCKNALFIMTSNI
GSQELADYCAKKGREVSKETVLSVVSPELKKYFSPEFINRIDDILPFVPLNTEDIVKIVGIQMRRVAQRLLERRVTLTWD
DSVILYLSEHGYDSSFGARPLKRLIQQKVVTLLSKALLKGDIKSDTSIELTMSRDVLLFKKVSG

Sequences:

>Translated_864_residues
MDKISDAVSEALEKAFELAKSAKNPYVSENHFLKCLLENTESLFYLIIKDIQSNPKLLITAVDEALSLEPSVVSGDAMPK
PSPGLQSLLLDARQTAKDLGDEYLSGDHVLLAFWKSNKEPFASWKKTVKVSLDDLKKLIINIRRGNRMDSPSAENNLRGL
EKYCKNLTSLAKDGKLDPVIGRDEEIRRTVQVLSRRTKNNPMLIGEPGVGKTAIAEGLALRIIQGDVPESLKGKQLYVLD
MGALIAGAKYRGEFEERLKSVLKDVESVDGESILFIDEVHTLVGAGATDGAMDAANLLKPALARGTLHCIGATTLNEYQK
YIEKDAALERRFQPIFVTEPSLEDAVFILRGLREKYEIFHGVRITEGALNAAVLLSYRYIPDRFLPDKAIDLIDEAASLI
RMQIGSLPLPIDEKERELAALIVKQEAIKREKAPSYQEEAEAMQQSIDQLKEELAVLRLRWDEEKKLISGLKEKKNSLEN
MKFSEEEAERVADYNRVAELRYSLIPALEEEIRQDEEALNQRDNRLLQEEVDERLIAQVVANWTGIPVQKMLEGEAEKLL
VLEESLEERVVGQPFAIAAVSDSIRAARVGLSDPQRPLGVFLFLGPTGVGKTELAKALADLLFNKEEAMVRFDMTEYMEK
HSVSKLIGSPPGYVGYEEGGSLSEALRRRPYSVVLFDEIEKADKEVFNILLQIFDEGILTDSKKRKVNCKNALFIMTSNI
GSQELADYCAKKGREVSKETVLSVVSPELKKYFSPEFINRIDDILPFVPLNTEDIVKIVGIQMRRVAQRLLERRVTLTWD
DSVILYLSEHGYDSSFGARPLKRLIQQKVVTLLSKALLKGDIKSDTSIELTMSRDVLLFKKVSG
>Mature_864_residues
MDKISDAVSEALEKAFELAKSAKNPYVSENHFLKCLLENTESLFYLIIKDIQSNPKLLITAVDEALSLEPSVVSGDAMPK
PSPGLQSLLLDARQTAKDLGDEYLSGDHVLLAFWKSNKEPFASWKKTVKVSLDDLKKLIINIRRGNRMDSPSAENNLRGL
EKYCKNLTSLAKDGKLDPVIGRDEEIRRTVQVLSRRTKNNPMLIGEPGVGKTAIAEGLALRIIQGDVPESLKGKQLYVLD
MGALIAGAKYRGEFEERLKSVLKDVESVDGESILFIDEVHTLVGAGATDGAMDAANLLKPALARGTLHCIGATTLNEYQK
YIEKDAALERRFQPIFVTEPSLEDAVFILRGLREKYEIFHGVRITEGALNAAVLLSYRYIPDRFLPDKAIDLIDEAASLI
RMQIGSLPLPIDEKERELAALIVKQEAIKREKAPSYQEEAEAMQQSIDQLKEELAVLRLRWDEEKKLISGLKEKKNSLEN
MKFSEEEAERVADYNRVAELRYSLIPALEEEIRQDEEALNQRDNRLLQEEVDERLIAQVVANWTGIPVQKMLEGEAEKLL
VLEESLEERVVGQPFAIAAVSDSIRAARVGLSDPQRPLGVFLFLGPTGVGKTELAKALADLLFNKEEAMVRFDMTEYMEK
HSVSKLIGSPPGYVGYEEGGSLSEALRRRPYSVVLFDEIEKADKEVFNILLQIFDEGILTDSKKRKVNCKNALFIMTSNI
GSQELADYCAKKGREVSKETVLSVVSPELKKYFSPEFINRIDDILPFVPLNTEDIVKIVGIQMRRVAQRLLERRVTLTWD
DSVILYLSEHGYDSSFGARPLKRLIQQKVVTLLSKALLKGDIKSDTSIELTMSRDVLLFKKVSG

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=317, Percent_Identity=38.801261829653, Blast_Score=226, Evalue=6e-59,
Organism=Escherichia coli, GI1788943, Length=855, Percent_Identity=49.5906432748538, Blast_Score=808, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=424, Percent_Identity=41.2735849056604, Blast_Score=295, Evalue=1e-80,
Organism=Saccharomyces cerevisiae, GI6320464, Length=707, Percent_Identity=50.4950495049505, Blast_Score=695, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=855, Percent_Identity=41.4035087719298, Blast_Score=619, Evalue=1e-178,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 96872; Mature: 96872

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKISDAVSEALEKAFELAKSAKNPYVSENHFLKCLLENTESLFYLIIKDIQSNPKLLIT
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEE
AVDEALSLEPSVVSGDAMPKPSPGLQSLLLDARQTAKDLGDEYLSGDHVLLAFWKSNKEP
EEHHHHCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCH
FASWKKTVKVSLDDLKKLIINIRRGNRMDSPSAENNLRGLEKYCKNLTSLAKDGKLDPVI
HHHHHHHHEECHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
GRDEEIRRTVQVLSRRTKNNPMLIGEPGVGKTAIAEGLALRIIQGDVPESLKGKQLYVLD
CCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHCCCEEEEECCCCCCCCCCCEEEEEE
MGALIAGAKYRGEFEERLKSVLKDVESVDGESILFIDEVHTLVGAGATDGAMDAANLLKP
HHHHHHCCHHCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCHHHHHHHHHH
ALARGTLHCIGATTLNEYQKYIEKDAALERRFQPIFVTEPSLEDAVFILRGLREKYEIFH
HHHCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHH
GVRITEGALNAAVLLSYRYIPDRFLPDKAIDLIDEAASLIRMQIGSLPLPIDEKERELAA
CCEEECHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
LIVKQEAIKREKAPSYQEEAEAMQQSIDQLKEELAVLRLRWDEEKKLISGLKEKKNSLEN
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
MKFSEEEAERVADYNRVAELRYSLIPALEEEIRQDEEALNQRDNRLLQEEVDERLIAQVV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ANWTGIPVQKMLEGEAEKLLVLEESLEERVVGQPFAIAAVSDSIRAARVGLSDPQRPLGV
HCCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCCCCCCEE
FLFLGPTGVGKTELAKALADLLFNKEEAMVRFDMTEYMEKHSVSKLIGSPPGYVGYEEGG
EEEECCCCCCHHHHHHHHHHHHHCCCHHHEEECHHHHHHHHHHHHHHCCCCCCCCCCCCC
SLSEALRRRPYSVVLFDEIEKADKEVFNILLQIFDEGILTDSKKRKVNCKNALFIMTSNI
CHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCC
GSQELADYCAKKGREVSKETVLSVVSPELKKYFSPEFINRIDDILPFVPLNTEDIVKIVG
CHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHH
IQMRRVAQRLLERRVTLTWDDSVILYLSEHGYDSSFGARPLKRLIQQKVVTLLSKALLKG
HHHHHHHHHHHHCCCEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
DIKSDTSIELTMSRDVLLFKKVSG
CCCCCCEEEEEECCCEEEEEECCC
>Mature Secondary Structure
MDKISDAVSEALEKAFELAKSAKNPYVSENHFLKCLLENTESLFYLIIKDIQSNPKLLIT
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEE
AVDEALSLEPSVVSGDAMPKPSPGLQSLLLDARQTAKDLGDEYLSGDHVLLAFWKSNKEP
EEHHHHCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCH
FASWKKTVKVSLDDLKKLIINIRRGNRMDSPSAENNLRGLEKYCKNLTSLAKDGKLDPVI
HHHHHHHHEECHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
GRDEEIRRTVQVLSRRTKNNPMLIGEPGVGKTAIAEGLALRIIQGDVPESLKGKQLYVLD
CCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHCCCEEEEECCCCCCCCCCCEEEEEE
MGALIAGAKYRGEFEERLKSVLKDVESVDGESILFIDEVHTLVGAGATDGAMDAANLLKP
HHHHHHCCHHCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCHHHHHHHHHH
ALARGTLHCIGATTLNEYQKYIEKDAALERRFQPIFVTEPSLEDAVFILRGLREKYEIFH
HHHCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHH
GVRITEGALNAAVLLSYRYIPDRFLPDKAIDLIDEAASLIRMQIGSLPLPIDEKERELAA
CCEEECHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
LIVKQEAIKREKAPSYQEEAEAMQQSIDQLKEELAVLRLRWDEEKKLISGLKEKKNSLEN
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
MKFSEEEAERVADYNRVAELRYSLIPALEEEIRQDEEALNQRDNRLLQEEVDERLIAQVV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ANWTGIPVQKMLEGEAEKLLVLEESLEERVVGQPFAIAAVSDSIRAARVGLSDPQRPLGV
HCCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCCCCCCEE
FLFLGPTGVGKTELAKALADLLFNKEEAMVRFDMTEYMEKHSVSKLIGSPPGYVGYEEGG
EEEECCCCCCHHHHHHHHHHHHHCCCHHHEEECHHHHHHHHHHHHHHCCCCCCCCCCCCC
SLSEALRRRPYSVVLFDEIEKADKEVFNILLQIFDEGILTDSKKRKVNCKNALFIMTSNI
CHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCC
GSQELADYCAKKGREVSKETVLSVVSPELKKYFSPEFINRIDDILPFVPLNTEDIVKIVG
CHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHH
IQMRRVAQRLLERRVTLTWDDSVILYLSEHGYDSSFGARPLKRLIQQKVVTLLSKALLKG
HHHHHHHHHHHHCCCEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
DIKSDTSIELTMSRDVLLFKKVSG
CCCCCCEEEEEECCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12682364 [H]