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The map label for this gene is tufA [H]

Identifier: 89898120

GI number: 89898120

Start: 359800

End: 360984

Strand: Direct

Name: tufA [H]

Synonym: CF0313

Alternate gene names: 89898120

Gene position: 359800-360984 (Clockwise)

Preceding gene: 89898119

Following gene: 89898121

Centisome position: 30.85

GC content: 42.36

Gene sequence:

>1185_bases
ATGTCAAAAGAAACTTTTCAACGTAATAAACCCCATATCAATATAGGGACCATTGGACACGTTGACCACGGTAAAACTAC
GCTAACAGCTGCAATTACACGCGCGCTGTCAGCAGAGGGGTTGGCTAATTTTTGCGATTACAGTTCTATTGACAATACTC
CTGAAGAAAAAGCTAGAGGAATTACTATCAACGCATCTCACGTTGAATACGAAACCCCTAACCGTCACTACGCTCACGTC
GACTGTCCTGGTCACGCTGACTACGTTAAAAATATGATTACAGGTGCAGCTCAAATGGACGGAGCAATTCTCGTTGTGTC
CGCTACTGACGGTGCGATGCCTCAAACAAAAGAACATATTCTTTTAGCAAGACAGGTTGGGGTTCCTTACATTGTTGTTT
TCCTCAACAAGATTGATATGATTTCTCAAGAGGATGCCGAGCTTGTTGACTTGGTTGAAATGGAGTTATCCGAGCTTCTA
GAAGAAAAAGGCTACAAAGGTTGCCCTATCATCCGTGGTTCTGCTTTGAAAGCTTTAGAAGGTGATGCAAGTTATGTTGA
AAAAATTCGCGAGCTAATGCAAGCAGTAGATGATAACATCCCTACGCCAGAGCGTGAAATTGATAAGCCTTTCTTAATGC
CTATCGAAGACGTCTTCTCTATTTCTGGTCGTGGTACAGTAGTAACTGGCCGTATTGAACGTGGAGTTGTTAAAGTTGGT
GATAAAGTACAGATCGTTGGTTTAAGAGATACCAGAGAAAGTATTGTTACCGGCGTAGAAATGTTCAGAAAAGAACTTCC
AGAAGGACAGGCAGGGGAGAACGTTGGATTGCTTCTCCGAGGTATCGGTAAGAATGATGTTGAGCGTGGTATGGTTATTT
GCCAACCTAACAGCGTAAAATCTCATACTCAATTTAAAGGTGCTGTTTATATCTTGCAAAAAGAAGAAGGTGGACGCCAT
AAGCCTTTCTTCACCGGATATAGACCTCAATTCTTCTTCCGTACAACAGACGTAACTGGTATTGTAAACCTTCCAGAAGG
AACAGAGATGGTTATGCCAGGGGATAACGTTGAAATCGATGTTCAATTAATTAGCCCCGTAGCTCTAGAAGAAGGTATGA
GATTTGCTATTCGTGAAGGTGGTCGTACAATCGGCGCTGGAACGATTTCAAAAATTATTGCCTAG

Upstream 100 bases:

>100_bases
TGGTAGAGCACTTGCATGGTAAGCAAGCGGTCGTAGGTTCAATTCCTATTCTGGGCAGAAGCAATGGTTGGAGTAAAATT
AATTTAATGAGGATTCTGAA

Downstream 100 bases:

>100_bases
TTTAGGTAATTTTATAAGCATGATTAGGGCGTCGGTCTTAATCATGCTTTTGGGTGTGTAGCTTAGTTGGTAGAGCAGTG
GCCTCCAAAGCCGCCGGTCG

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MSKETFQRNKPHINIGTIGHVDHGKTTLTAAITRALSAEGLANFCDYSSIDNTPEEKARGITINASHVEYETPNRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSATDGAMPQTKEHILLARQVGVPYIVVFLNKIDMISQEDAELVDLVEMELSELL
EEKGYKGCPIIRGSALKALEGDASYVEKIRELMQAVDDNIPTPEREIDKPFLMPIEDVFSISGRGTVVTGRIERGVVKVG
DKVQIVGLRDTRESIVTGVEMFRKELPEGQAGENVGLLLRGIGKNDVERGMVICQPNSVKSHTQFKGAVYILQKEEGGRH
KPFFTGYRPQFFFRTTDVTGIVNLPEGTEMVMPGDNVEIDVQLISPVALEEGMRFAIREGGRTIGAGTISKIIA

Sequences:

>Translated_394_residues
MSKETFQRNKPHINIGTIGHVDHGKTTLTAAITRALSAEGLANFCDYSSIDNTPEEKARGITINASHVEYETPNRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSATDGAMPQTKEHILLARQVGVPYIVVFLNKIDMISQEDAELVDLVEMELSELL
EEKGYKGCPIIRGSALKALEGDASYVEKIRELMQAVDDNIPTPEREIDKPFLMPIEDVFSISGRGTVVTGRIERGVVKVG
DKVQIVGLRDTRESIVTGVEMFRKELPEGQAGENVGLLLRGIGKNDVERGMVICQPNSVKSHTQFKGAVYILQKEEGGRH
KPFFTGYRPQFFFRTTDVTGIVNLPEGTEMVMPGDNVEIDVQLISPVALEEGMRFAIREGGRTIGAGTISKIIA
>Mature_393_residues
SKETFQRNKPHINIGTIGHVDHGKTTLTAAITRALSAEGLANFCDYSSIDNTPEEKARGITINASHVEYETPNRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSATDGAMPQTKEHILLARQVGVPYIVVFLNKIDMISQEDAELVDLVEMELSELLE
EKGYKGCPIIRGSALKALEGDASYVEKIRELMQAVDDNIPTPEREIDKPFLMPIEDVFSISGRGTVVTGRIERGVVKVGD
KVQIVGLRDTRESIVTGVEMFRKELPEGQAGENVGLLLRGIGKNDVERGMVICQPNSVKSHTQFKGAVYILQKEEGGRHK
PFFTGYRPQFFFRTTDVTGIVNLPEGTEMVMPGDNVEIDVQLISPVALEEGMRFAIREGGRTIGAGTISKIIA

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=397, Percent_Identity=54.1561712846348, Blast_Score=453, Evalue=1e-127,
Organism=Homo sapiens, GI223555963, Length=440, Percent_Identity=28.8636363636364, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI5729864, Length=440, Percent_Identity=28.8636363636364, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI4503475, Length=446, Percent_Identity=28.47533632287, Blast_Score=159, Evalue=6e-39,
Organism=Homo sapiens, GI4503471, Length=449, Percent_Identity=29.1759465478842, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI54607086, Length=320, Percent_Identity=29.375, Blast_Score=139, Evalue=6e-33,
Organism=Homo sapiens, GI194018522, Length=436, Percent_Identity=26.3761467889908, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI194018520, Length=436, Percent_Identity=26.3761467889908, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI194097354, Length=436, Percent_Identity=26.3761467889908, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI46094014, Length=436, Percent_Identity=26.1467889908257, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI4503507, Length=287, Percent_Identity=28.5714285714286, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI94966754, Length=145, Percent_Identity=35.8620689655172, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI157426893, Length=377, Percent_Identity=26.525198938992, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI310132016, Length=120, Percent_Identity=35.8333333333333, Blast_Score=66, Evalue=5e-11,
Organism=Homo sapiens, GI310110807, Length=120, Percent_Identity=35.8333333333333, Blast_Score=66, Evalue=5e-11,
Organism=Homo sapiens, GI310123363, Length=120, Percent_Identity=35.8333333333333, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1790412, Length=396, Percent_Identity=67.1717171717172, Blast_Score=551, Evalue=1e-158,
Organism=Escherichia coli, GI1789737, Length=396, Percent_Identity=67.9292929292929, Blast_Score=550, Evalue=1e-158,
Organism=Escherichia coli, GI2367247, Length=369, Percent_Identity=30.3523035230352, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI48994988, Length=235, Percent_Identity=34.0425531914894, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1789108, Length=440, Percent_Identity=25, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788922, Length=269, Percent_Identity=27.5092936802974, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17556456, Length=401, Percent_Identity=50.8728179551122, Blast_Score=395, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI25141371, Length=388, Percent_Identity=44.5876288659794, Blast_Score=334, Evalue=4e-92,
Organism=Caenorhabditis elegans, GI17552884, Length=452, Percent_Identity=28.5398230088496, Blast_Score=161, Evalue=7e-40,
Organism=Caenorhabditis elegans, GI17569207, Length=452, Percent_Identity=28.5398230088496, Blast_Score=161, Evalue=7e-40,
Organism=Caenorhabditis elegans, GI32566303, Length=422, Percent_Identity=28.9099526066351, Blast_Score=141, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI115532067, Length=445, Percent_Identity=26.7415730337079, Blast_Score=131, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI115532065, Length=445, Percent_Identity=26.7415730337079, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI32566629, Length=436, Percent_Identity=25.4587155963303, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17506081, Length=299, Percent_Identity=28.0936454849498, Blast_Score=114, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI17557151, Length=263, Percent_Identity=30.0380228136882, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI32566301, Length=147, Percent_Identity=36.0544217687075, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17509919, Length=286, Percent_Identity=25.8741258741259, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=397, Percent_Identity=62.72040302267, Blast_Score=508, Evalue=1e-145,
Organism=Saccharomyces cerevisiae, GI6325337, Length=441, Percent_Identity=28.7981859410431, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6319594, Length=441, Percent_Identity=28.7981859410431, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6320377, Length=353, Percent_Identity=27.7620396600567, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6322937, Length=457, Percent_Identity=23.6323851203501, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6320863, Length=294, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=239, Percent_Identity=32.2175732217573, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323098, Length=154, Percent_Identity=31.1688311688312, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI281363316, Length=395, Percent_Identity=57.9746835443038, Blast_Score=473, Evalue=1e-134,
Organism=Drosophila melanogaster, GI17864358, Length=395, Percent_Identity=57.9746835443038, Blast_Score=473, Evalue=1e-134,
Organism=Drosophila melanogaster, GI19921738, Length=388, Percent_Identity=49.4845360824742, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24652838, Length=446, Percent_Identity=27.8026905829596, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI17137572, Length=446, Percent_Identity=27.8026905829596, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI45553807, Length=446, Percent_Identity=27.3542600896861, Blast_Score=148, Evalue=6e-36,
Organism=Drosophila melanogaster, GI45553816, Length=446, Percent_Identity=27.3542600896861, Blast_Score=148, Evalue=6e-36,
Organism=Drosophila melanogaster, GI24651721, Length=446, Percent_Identity=27.3542600896861, Blast_Score=148, Evalue=6e-36,
Organism=Drosophila melanogaster, GI17864154, Length=446, Percent_Identity=27.3542600896861, Blast_Score=148, Evalue=6e-36,
Organism=Drosophila melanogaster, GI45550900, Length=338, Percent_Identity=31.0650887573964, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI19922690, Length=300, Percent_Identity=31, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI17137380, Length=345, Percent_Identity=28.9855072463768, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24647046, Length=279, Percent_Identity=29.0322580645161, Blast_Score=95, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24647048, Length=279, Percent_Identity=29.0322580645161, Blast_Score=95, Evalue=8e-20,
Organism=Drosophila melanogaster, GI19922512, Length=439, Percent_Identity=22.7790432801822, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24654852, Length=439, Percent_Identity=22.7790432801822, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI28572034, Length=138, Percent_Identity=39.8550724637681, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI78706572, Length=233, Percent_Identity=29.1845493562232, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43192; Mature: 43061

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKETFQRNKPHINIGTIGHVDHGKTTLTAAITRALSAEGLANFCDYSSIDNTPEEKARG
CCCCCCCCCCCEEEECEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCC
ITINASHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSATDGAMPQTKEHI
EEEECCEEEECCCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVGVPYIVVFLNKIDMISQEDAELVDLVEMELSELLEEKGYKGCPIIRGSALKALE
EEEHHCCCCEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEECCCCHHHCC
GDASYVEKIRELMQAVDDNIPTPEREIDKPFLMPIEDVFSISGRGTVVTGRIERGVVKVG
CCHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCHHHHHCCCCCCEEEEECCCCCEEECC
DKVQIVGLRDTRESIVTGVEMFRKELPEGQAGENVGLLLRGIGKNDVERGMVICQPNSVK
CEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHCCEEEECCCCCC
SHTQFKGAVYILQKEEGGRHKPFFTGYRPQFFFRTTDVTGIVNLPEGTEMVMPGDNVEID
CCCCCCEEEEEEEECCCCCCCCEECCCCCEEEEEECCCEEEEECCCCCEEEECCCCCEEE
VQLISPVALEEGMRFAIREGGRTIGAGTISKIIA
EEEECCHHHHHCCEEEECCCCCEECCHHHHHHCC
>Mature Secondary Structure 
SKETFQRNKPHINIGTIGHVDHGKTTLTAAITRALSAEGLANFCDYSSIDNTPEEKARG
CCCCCCCCCCEEEECEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCC
ITINASHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSATDGAMPQTKEHI
EEEECCEEEECCCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVGVPYIVVFLNKIDMISQEDAELVDLVEMELSELLEEKGYKGCPIIRGSALKALE
EEEHHCCCCEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEECCCCHHHCC
GDASYVEKIRELMQAVDDNIPTPEREIDKPFLMPIEDVFSISGRGTVVTGRIERGVVKVG
CCHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCHHHHHCCCCCCEEEEECCCCCEEECC
DKVQIVGLRDTRESIVTGVEMFRKELPEGQAGENVGLLLRGIGKNDVERGMVICQPNSVK
CEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHCCEEEECCCCCC
SHTQFKGAVYILQKEEGGRHKPFFTGYRPQFFFRTTDVTGIVNLPEGTEMVMPGDNVEID
CCCCCCEEEEEEEECCCCCCCCEECCCCCEEEEEECCCEEEEECCCCCEEEECCCCCEEE
VQLISPVALEEGMRFAIREGGRTIGAGTISKIIA
EEEECCHHHHHCCEEEECCCCCEECCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA