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The map label for this gene is ispE

Identifier: 89898006

GI number: 89898006

Start: 230257

End: 231129

Strand: Direct

Name: ispE

Synonym: CF0199

Alternate gene names: 89898006

Gene position: 230257-231129 (Clockwise)

Preceding gene: 89898005

Following gene: 89898008

Centisome position: 19.74

GC content: 39.63

Gene sequence:

>873_bases
ATGGATCTTTTTTCTCCAGCAAAACTGAATCTTTTTTTAAAGCTTCACGGAAAAACTTCTCATGGTTTTCATGAGATGAC
CACTCAGTATCAAGTCATTGACTTCGGAGACAATCTTTATTTAGAGAGTAGTAATGAAGACTCTCTTGTATGTAATCTCC
CAGAATTAAATACCCCACAGAATCTCATATGGAAAAGTCTTCAGGTTTTCAGAGACTATACACAGATACGTAGTCCTGTA
GCTTGGCGGTTACATAAGCGAATCCCTATCGGAGCTGGTGTGGGAGGCGGTAGCAGCAACGCTGCAACAGCCCTTTATGC
TTTAAACGAGCATTTTCAAACACAACTACCCAATAGTGTTCTTCAGGAATTAGGAAAAAAAATTGGTATGGACGTGCCTC
TGTTTTTTTCTTCAGGGTCCGCTATAGGAGTAGGATGTGGTGAGAAAATCCTTCCTTATAAAGACTGCAAAAGAGAAGAG
AGATTCGTACTATATTTTTCTGATCAAGGAGTCCTTACTAAAGATGCCTTTTCTTACGTACGTTCCGAAGACTTCTCTCA
AAGGGAAGAAAATATCTCTTTATACAAAAAAGAAAATGATTTAGAGAAGTCTGTATTTCGTTTCCGTCGGGATCTGGAAG
AGAAAAAACAAATGCTAAAAAAGATGTGGAGTCCTTTTCATGCTCATGTCTGTATGTCCGGAGCTGGGGCAACTTTATTT
GTAAGCTATCCCAGGAAACTGGAAATAGATCCTTCCATTGCCAAAGCTATTCACGCAACTATCCAAAATAGTCAAGGTGT
GCTTGTAAATAGCCTACGCAAACACAGCGGGTGGTTCCCGTGTCCCGATAATTTATTTGCAACAACAAGATAA

Upstream 100 bases:

>100_bases
ATCAACAGTCGTCTCAAGAACAGACGGTTGCTGCTGAAGAGTTGGAGTAGACATCCTTGGGTGAGGAGCTTTGCTCCTTG
CCTATACAACAATTGGTTTT

Downstream 100 bases:

>100_bases
CAAAGCTCCTTCAGCAATAGAAGCGATCTTACTGGCTTCTACAGAAAGAGTGCTCTCGGCTTGGCAAACTTCGTAAGATT
GATCGTCTTTTCCCTGCTCG

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MDLFSPAKLNLFLKLHGKTSHGFHEMTTQYQVIDFGDNLYLESSNEDSLVCNLPELNTPQNLIWKSLQVFRDYTQIRSPV
AWRLHKRIPIGAGVGGGSSNAATALYALNEHFQTQLPNSVLQELGKKIGMDVPLFFSSGSAIGVGCGEKILPYKDCKREE
RFVLYFSDQGVLTKDAFSYVRSEDFSQREENISLYKKENDLEKSVFRFRRDLEEKKQMLKKMWSPFHAHVCMSGAGATLF
VSYPRKLEIDPSIAKAIHATIQNSQGVLVNSLRKHSGWFPCPDNLFATTR

Sequences:

>Translated_290_residues
MDLFSPAKLNLFLKLHGKTSHGFHEMTTQYQVIDFGDNLYLESSNEDSLVCNLPELNTPQNLIWKSLQVFRDYTQIRSPV
AWRLHKRIPIGAGVGGGSSNAATALYALNEHFQTQLPNSVLQELGKKIGMDVPLFFSSGSAIGVGCGEKILPYKDCKREE
RFVLYFSDQGVLTKDAFSYVRSEDFSQREENISLYKKENDLEKSVFRFRRDLEEKKQMLKKMWSPFHAHVCMSGAGATLF
VSYPRKLEIDPSIAKAIHATIQNSQGVLVNSLRKHSGWFPCPDNLFATTR
>Mature_290_residues
MDLFSPAKLNLFLKLHGKTSHGFHEMTTQYQVIDFGDNLYLESSNEDSLVCNLPELNTPQNLIWKSLQVFRDYTQIRSPV
AWRLHKRIPIGAGVGGGSSNAATALYALNEHFQTQLPNSVLQELGKKIGMDVPLFFSSGSAIGVGCGEKILPYKDCKREE
RFVLYFSDQGVLTKDAFSYVRSEDFSQREENISLYKKENDLEKSVFRFRRDLEEKKQMLKKMWSPFHAHVCMSGAGATLF
VSYPRKLEIDPSIAKAIHATIQNSQGVLVNSLRKHSGWFPCPDNLFATTR

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=249, Percent_Identity=30.1204819277108, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_CHLFF (Q255R7)

Other databases:

- EMBL:   AP006861
- RefSeq:   YP_515116.1
- ProteinModelPortal:   Q255R7
- SMR:   Q255R7
- STRING:   Q255R7
- GeneID:   3958701
- GenomeReviews:   AP006861_GR
- KEGG:   cfe:CF0199
- eggNOG:   COG1947
- HOGENOM:   HBG413867
- OMA:   GFHEMTT
- ProtClustDB:   PRK00650
- BioCyc:   CFEL264202:CF0199-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 32892; Mature: 32892

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: NA

Important sites: ACT_SITE 8-8 ACT_SITE 131-131

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLFSPAKLNLFLKLHGKTSHGFHEMTTQYQVIDFGDNLYLESSNEDSLVCNLPELNTPQ
CCCCCCCEEEEEEEEECCCCCCHHHHCCEEEEEECCCEEEEECCCCCCEEEECCCCCCCH
NLIWKSLQVFRDYTQIRSPVAWRLHKRIPIGAGVGGGSSNAATALYALNEHFQTQLPNSV
HHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHH
LQELGKKIGMDVPLFFSSGSAIGVGCGEKILPYKDCKREERFVLYFSDQGVLTKDAFSYV
HHHHHHHHCCCCEEEECCCCEEEECCCCCCCCHHHCCCCCEEEEEECCCCCCHHHHHHHH
RSEDFSQREENISLYKKENDLEKSVFRFRRDLEEKKQMLKKMWSPFHAHVCMSGAGATLF
HCCCHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEECCCCCEEE
VSYPRKLEIDPSIAKAIHATIQNSQGVLVNSLRKHSGWFPCPDNLFATTR
EECCCEECCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDLFSPAKLNLFLKLHGKTSHGFHEMTTQYQVIDFGDNLYLESSNEDSLVCNLPELNTPQ
CCCCCCCEEEEEEEEECCCCCCHHHHCCEEEEEECCCEEEEECCCCCCEEEECCCCCCCH
NLIWKSLQVFRDYTQIRSPVAWRLHKRIPIGAGVGGGSSNAATALYALNEHFQTQLPNSV
HHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHH
LQELGKKIGMDVPLFFSSGSAIGVGCGEKILPYKDCKREERFVLYFSDQGVLTKDAFSYV
HHHHHHHHCCCCEEEECCCCEEEECCCCCCCCHHHCCCCCEEEEEECCCCCCHHHHHHHH
RSEDFSQREENISLYKKENDLEKSVFRFRRDLEEKKQMLKKMWSPFHAHVCMSGAGATLF
HCCCHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEECCCCCEEE
VSYPRKLEIDPSIAKAIHATIQNSQGVLVNSLRKHSGWFPCPDNLFATTR
EECCCEECCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA