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The map label for this gene is dnaG [H]

Identifier: 89897993

GI number: 89897993

Start: 217847

End: 219637

Strand: Direct

Name: dnaG [H]

Synonym: CF0186

Alternate gene names: 89897993

Gene position: 217847-219637 (Clockwise)

Preceding gene: 89897990

Following gene: 89897994

Centisome position: 18.68

GC content: 39.87

Gene sequence:

>1791_bases
ATGTATACAGAAGAAAGTTTAGATAATCTTAGGCACAACATTGATATTGTGGAAGTGCTTTCGGAACATCTTCATTTAAA
AAGGAGTGGCGCTACCTATAAAGCGTGTTGCCCCTTCCATGTAGAGAAAACGCCCTCGTTTATTGTTAATCCGACATGTG
CGTATTACCATTGTTTTGGTTGTGGAGCTCACGGTGATGCTATTAGTTTTTTGATGAATCATCTTGGGTATTCTTTTAGT
GAAGCCGTTCTTACTTTATCAAAAAAATTTCACGTAGATCTTGTCGTCGAAGTTAAGGATACGCAGACCTCATTTCCTAT
AGGGGCGAAAGATGAGTTACGACAGATTAACCATGAAGCAGAAAACCTATTTCGTTTTTGTTTATACCAGCTTGCAGAGG
GTAGGGAAGCCTTGCAGTATTTATATCGTCGGGGTTTTTCTCCAGATACAATAGATCGTTTTCATTTAGGCTACGCACCA
GAGCAAACGTTATTTGTTCAGGCTATGCGTGAAAAAAATATTTCAGAAAAACAGCTAGAATCAGCAGGCTTTATCGGAAA
TAAGTGGTTTTTATTTTCTCGTAGAATCATTTTCCCCGTACACGATTCTCTAGGACATACTATAGGGTTTTCATCAAGAA
AGTTTTTAGAGAATACTCGAGGGAGTAAGTATGTGAATACTCCAGAAACAATTATCTTTAAAAAATCTCGTGCTCTTTTC
GGATTACATTTATCGCGAAGAAGAATTGCAAAGGAAAAACGTGTGATTCTAGTCGAGGGGCAGGCAGATTGCCTGCAGAT
GATAGATTCAGGATTTAATTGTACTCTTGCTGCTCAGGGAACTTCGTTTACCGAGGATCACGTAAAAGAATTAAGTAAAT
TAGGGGTTCTTAAGGCTTATGTCCTGTTTGATGGTGATGACGCTGGAATAAAGGCGGCTATTCGTGTTGGAGATATGTGC
CAGACAGTGGGTATTGCTGTTATGGTTTGTCGTCTCCCCCAAGGGGAGGATCCCGATTCCTTCCTGAGACATAAGGGAGC
AGGACCTCTTGGTGAACTTCTGGATCAAAGTGAGGATTACCTTACCTTCCTTATCAGTGAAAAAATTCGCGTCTATCCGA
ATTTTTCTCCGAGAGAAAAGGCTCTGGTAATCGAAGAAACCATTACACAAATTAAAAAATGGGGGAATCCCATTGTTGTG
TACGAACATCTTAAGCAATTAGCTTCTTTGATGATGATTCCAGAAAGTATGGTATTTTCTTTAGCAAAATTAGAATCGGG
GATCACACCAGCTACAGTCCTGGCAACGAGTAAAGCAAAGTTACCGAAAATTCATCCCGATGTGATTATTGAAACTGATG
TTTTACGCTGTATGTTGTTTTGCAAAGCTCATAACACGAGCCTCCCCTACACAGCAAAAAATTATTTTACTCCTAAGGAT
TTCAAACATCCGGAATGCAGAAAGTTGTTTTCTCGTCTCATCGAATATTATGAAAAACATCAAAGTAATCTTCCTTTTGA
CGAAGCCTTATCCTTGTTAGAAGACAAGGTCATTATAGAACTGCTTATAAAACGGCGTATGAATACTGATTGTCTGGAGA
CTGTATTTATACAGTCTTTACAAAAGCTTGCGGATCGTCAATGGAAAGAGCAGCGCCATCCTCTTTCTGCGCAGTCAAAA
AATTCTCAGGGGCACCAGCTCTCTATTCTCGAAGACTATGTTCGCTTGCGTAAAGACCGAGTCATTGTTTCCCTCCTAGA
TCCTCAAGACTCCCAACCCTGTATTATTTAG

Upstream 100 bases:

>100_bases
TATCTTAGAGATGTGTTGGTCACAGATTCATGCAATGAGCCGTCTTTCTTTTAAAGAGGTGAATGTGGCATAACCAAGGA
ACAGCCAAGCTTATAAATCT

Downstream 100 bases:

>100_bases
GGTTTTCCAAAGGGCAATTCGAATTATTAACTTTTTGTAAAGGTTGAAAAATTAATAAGTTTAATATAAAATATTGCCTT
GTTAGTTTATTAATTTGTTG

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 596; Mature: 596

Protein sequence:

>596_residues
MYTEESLDNLRHNIDIVEVLSEHLHLKRSGATYKACCPFHVEKTPSFIVNPTCAYYHCFGCGAHGDAISFLMNHLGYSFS
EAVLTLSKKFHVDLVVEVKDTQTSFPIGAKDELRQINHEAENLFRFCLYQLAEGREALQYLYRRGFSPDTIDRFHLGYAP
EQTLFVQAMREKNISEKQLESAGFIGNKWFLFSRRIIFPVHDSLGHTIGFSSRKFLENTRGSKYVNTPETIIFKKSRALF
GLHLSRRRIAKEKRVILVEGQADCLQMIDSGFNCTLAAQGTSFTEDHVKELSKLGVLKAYVLFDGDDAGIKAAIRVGDMC
QTVGIAVMVCRLPQGEDPDSFLRHKGAGPLGELLDQSEDYLTFLISEKIRVYPNFSPREKALVIEETITQIKKWGNPIVV
YEHLKQLASLMMIPESMVFSLAKLESGITPATVLATSKAKLPKIHPDVIIETDVLRCMLFCKAHNTSLPYTAKNYFTPKD
FKHPECRKLFSRLIEYYEKHQSNLPFDEALSLLEDKVIIELLIKRRMNTDCLETVFIQSLQKLADRQWKEQRHPLSAQSK
NSQGHQLSILEDYVRLRKDRVIVSLLDPQDSQPCII

Sequences:

>Translated_596_residues
MYTEESLDNLRHNIDIVEVLSEHLHLKRSGATYKACCPFHVEKTPSFIVNPTCAYYHCFGCGAHGDAISFLMNHLGYSFS
EAVLTLSKKFHVDLVVEVKDTQTSFPIGAKDELRQINHEAENLFRFCLYQLAEGREALQYLYRRGFSPDTIDRFHLGYAP
EQTLFVQAMREKNISEKQLESAGFIGNKWFLFSRRIIFPVHDSLGHTIGFSSRKFLENTRGSKYVNTPETIIFKKSRALF
GLHLSRRRIAKEKRVILVEGQADCLQMIDSGFNCTLAAQGTSFTEDHVKELSKLGVLKAYVLFDGDDAGIKAAIRVGDMC
QTVGIAVMVCRLPQGEDPDSFLRHKGAGPLGELLDQSEDYLTFLISEKIRVYPNFSPREKALVIEETITQIKKWGNPIVV
YEHLKQLASLMMIPESMVFSLAKLESGITPATVLATSKAKLPKIHPDVIIETDVLRCMLFCKAHNTSLPYTAKNYFTPKD
FKHPECRKLFSRLIEYYEKHQSNLPFDEALSLLEDKVIIELLIKRRMNTDCLETVFIQSLQKLADRQWKEQRHPLSAQSK
NSQGHQLSILEDYVRLRKDRVIVSLLDPQDSQPCII
>Mature_596_residues
MYTEESLDNLRHNIDIVEVLSEHLHLKRSGATYKACCPFHVEKTPSFIVNPTCAYYHCFGCGAHGDAISFLMNHLGYSFS
EAVLTLSKKFHVDLVVEVKDTQTSFPIGAKDELRQINHEAENLFRFCLYQLAEGREALQYLYRRGFSPDTIDRFHLGYAP
EQTLFVQAMREKNISEKQLESAGFIGNKWFLFSRRIIFPVHDSLGHTIGFSSRKFLENTRGSKYVNTPETIIFKKSRALF
GLHLSRRRIAKEKRVILVEGQADCLQMIDSGFNCTLAAQGTSFTEDHVKELSKLGVLKAYVLFDGDDAGIKAAIRVGDMC
QTVGIAVMVCRLPQGEDPDSFLRHKGAGPLGELLDQSEDYLTFLISEKIRVYPNFSPREKALVIEETITQIKKWGNPIVV
YEHLKQLASLMMIPESMVFSLAKLESGITPATVLATSKAKLPKIHPDVIIETDVLRCMLFCKAHNTSLPYTAKNYFTPKD
FKHPECRKLFSRLIEYYEKHQSNLPFDEALSLLEDKVIIELLIKRRMNTDCLETVFIQSLQKLADRQWKEQRHPLSAQSK
NSQGHQLSILEDYVRLRKDRVIVSLLDPQDSQPCII

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=369, Percent_Identity=34.6883468834688, Blast_Score=210, Evalue=2e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 68022; Mature: 68022

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYTEESLDNLRHNIDIVEVLSEHLHLKRSGATYKACCPFHVEKTPSFIVNPTCAYYHCFG
CCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEECCCCCEEEEEC
CGAHGDAISFLMNHLGYSFSEAVLTLSKKFHVDLVVEVKDTQTSFPIGAKDELRQINHEA
CCCCHHHHHHHHHHHCCHHHHHHHHHHHCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHH
ENLFRFCLYQLAEGREALQYLYRRGFSPDTIDRFHLGYAPEQTLFVQAMREKNISEKQLE
HHHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHHCCCCHHHHH
SAGFIGNKWFLFSRRIIFPVHDSLGHTIGFSSRKFLENTRGSKYVNTPETIIFKKSRALF
HCCCCCCEEEEEECEEEEECHHHCCCCCCCCCHHHHHHCCCCCCCCCCCEEEEECCCHHH
GLHLSRRRIAKEKRVILVEGQADCLQMIDSGFNCTLAAQGTSFTEDHVKELSKLGVLKAY
HHHHHHHHHHCCCCEEEEECCHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCEEEE
VLFDGDDAGIKAAIRVGDMCQTVGIAVMVCRLPQGEDPDSFLRHKGAGPLGELLDQSEDY
EEEECCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHCCCCCHHHHHCCCCHH
LTFLISEKIRVYPNFSPREKALVIEETITQIKKWGNPIVVYEHLKQLASLMMIPESMVFS
HHHHHHCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCHHHHHH
LAKLESGITPATVLATSKAKLPKIHPDVIIETDVLRCMLFCKAHNTSLPYTAKNYFTPKD
HHHHHCCCCCHHEEEECCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
FKHPECRKLFSRLIEYYEKHQSNLPFDEALSLLEDKVIIELLIKRRMNTDCLETVFIQSL
CCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
QKLADRQWKEQRHPLSAQSKNSQGHQLSILEDYVRLRKDRVIVSLLDPQDSQPCII
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCC
>Mature Secondary Structure
MYTEESLDNLRHNIDIVEVLSEHLHLKRSGATYKACCPFHVEKTPSFIVNPTCAYYHCFG
CCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEECCCCCEEEEEC
CGAHGDAISFLMNHLGYSFSEAVLTLSKKFHVDLVVEVKDTQTSFPIGAKDELRQINHEA
CCCCHHHHHHHHHHHCCHHHHHHHHHHHCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHH
ENLFRFCLYQLAEGREALQYLYRRGFSPDTIDRFHLGYAPEQTLFVQAMREKNISEKQLE
HHHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHHCCCCHHHHH
SAGFIGNKWFLFSRRIIFPVHDSLGHTIGFSSRKFLENTRGSKYVNTPETIIFKKSRALF
HCCCCCCEEEEEECEEEEECHHHCCCCCCCCCHHHHHHCCCCCCCCCCCEEEEECCCHHH
GLHLSRRRIAKEKRVILVEGQADCLQMIDSGFNCTLAAQGTSFTEDHVKELSKLGVLKAY
HHHHHHHHHHCCCCEEEEECCHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCEEEE
VLFDGDDAGIKAAIRVGDMCQTVGIAVMVCRLPQGEDPDSFLRHKGAGPLGELLDQSEDY
EEEECCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHCCCCCHHHHHCCCCHH
LTFLISEKIRVYPNFSPREKALVIEETITQIKKWGNPIVVYEHLKQLASLMMIPESMVFS
HHHHHHCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCHHHHHH
LAKLESGITPATVLATSKAKLPKIHPDVIIETDVLRCMLFCKAHNTSLPYTAKNYFTPKD
HHHHHCCCCCHHEEEECCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
FKHPECRKLFSRLIEYYEKHQSNLPFDEALSLLEDKVIIELLIKRRMNTDCLETVFIQSL
CCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
QKLADRQWKEQRHPLSAQSKNSQGHQLSILEDYVRLRKDRVIVSLLDPQDSQPCII
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]